Wednesday, September 5
... blood cells. To obtain sufficient amounts of the protein, you decide to clone the β-globin gene. Would you construct a genomic library or a cDNA library? What material would you use as a source of DNA or RNA? ...
... blood cells. To obtain sufficient amounts of the protein, you decide to clone the β-globin gene. Would you construct a genomic library or a cDNA library? What material would you use as a source of DNA or RNA? ...
Practical molecular biology
... Type III enzymes recognize two separate non-palindromic sequences that are inversely oriented. They cut DNA about 20-30 base pairs after the recognition site. ...
... Type III enzymes recognize two separate non-palindromic sequences that are inversely oriented. They cut DNA about 20-30 base pairs after the recognition site. ...
Bio07_TR__U04_CH13.QXD
... 2. Crossing dissimilar individuals to bring together the best of both Organisms is called ________________________ . 3. The continued breeding of individuals with similar characteristics is called _______________________ . 4. Biologists change the DNA code of a living organism through ______________ ...
... 2. Crossing dissimilar individuals to bring together the best of both Organisms is called ________________________ . 3. The continued breeding of individuals with similar characteristics is called _______________________ . 4. Biologists change the DNA code of a living organism through ______________ ...
Extra Chromosomal Elements
... Natural plasmids are present naturally in bacterial and some yeast cells carrying genes for its own replication and genes for some functions of the cells like F- plasmid (F-pili during conjugation and some R-plasmids for drug resistance). Artificial plasmids are naturally present plasmid but designe ...
... Natural plasmids are present naturally in bacterial and some yeast cells carrying genes for its own replication and genes for some functions of the cells like F- plasmid (F-pili during conjugation and some R-plasmids for drug resistance). Artificial plasmids are naturally present plasmid but designe ...
Lab Quiz 4 Key
... 2. What is the function of PCR (polymerase chain reaction)? (0.5 pt) [To produce many copies of DNA from only a small amount] ...
... 2. What is the function of PCR (polymerase chain reaction)? (0.5 pt) [To produce many copies of DNA from only a small amount] ...
Exp 4 Lecture - Seattle Central College
... • Genetic transformation involves the insertion of some new DNA into the E. coli cells. In addition to one large chromosome, bacteria often contain one or more small circular pieces of DNA called plasmids. • Plasmid DNA usually contains genes for more than one trait. Genetic engineering involves ins ...
... • Genetic transformation involves the insertion of some new DNA into the E. coli cells. In addition to one large chromosome, bacteria often contain one or more small circular pieces of DNA called plasmids. • Plasmid DNA usually contains genes for more than one trait. Genetic engineering involves ins ...
Producing the Bovine Growth Hormone
... including bovine growth hormone, or BGH. This DNA is found in the chromosomes in each cell's nucleus. To clone the BGH gene, DNA is taken from the cow cell's nucleus and cut with a restriction enzyme that leaves “sticky ends” on either side of the BGH gene. “Sticky ends” are so-named because they ar ...
... including bovine growth hormone, or BGH. This DNA is found in the chromosomes in each cell's nucleus. To clone the BGH gene, DNA is taken from the cow cell's nucleus and cut with a restriction enzyme that leaves “sticky ends” on either side of the BGH gene. “Sticky ends” are so-named because they ar ...
Molecular Cloning
... In order to have enough DNA to work with for a single gene or sequence, you must have a way to “clone”, or reproduce many exact copies of that gene. This is called “molecular cloning” ...
... In order to have enough DNA to work with for a single gene or sequence, you must have a way to “clone”, or reproduce many exact copies of that gene. This is called “molecular cloning” ...
Prodigiosin Production in E. Coli
... We will choose a plasmid with specific antibiotic resistances Some strains of S. marcescens are known to be resistant to a number of antibiotics naturally The restriction site will be cut by TBD restriction enzyme ...
... We will choose a plasmid with specific antibiotic resistances Some strains of S. marcescens are known to be resistant to a number of antibiotics naturally The restriction site will be cut by TBD restriction enzyme ...
BIOCHEMISTRY 4.1 HOMEWORK
... b. Draw the structures resulting from the reaction of this end sequence DNA polymerase I and four deoxynucloside triphosphases (see Fig 8-36 in the book!) ...
... b. Draw the structures resulting from the reaction of this end sequence DNA polymerase I and four deoxynucloside triphosphases (see Fig 8-36 in the book!) ...
E. Coli
... (DNA). i.e. the act of putting foreign DNA into a bacterial cell Occurs in nature, but rarely If the foreign DNA has an origin of replication recognized by the host cell DNA polymerases, the bacteria will replicate the foreign DNA along with their own DNA. ...
... (DNA). i.e. the act of putting foreign DNA into a bacterial cell Occurs in nature, but rarely If the foreign DNA has an origin of replication recognized by the host cell DNA polymerases, the bacteria will replicate the foreign DNA along with their own DNA. ...
Restriction Enzymes, Vectors, and Genetic Libraries
... Creates either sticky ends or blunt ends ...
... Creates either sticky ends or blunt ends ...
Frontiers of Genetics
... species, into a single DNA molecule • Bacteria have small circular pieces of DNA called plasmids separate from their larger single chromosome • Plasmids can replicate and pass between bacterial cells allowing gene sharing – associated with antibacterial resistance ...
... species, into a single DNA molecule • Bacteria have small circular pieces of DNA called plasmids separate from their larger single chromosome • Plasmids can replicate and pass between bacterial cells allowing gene sharing – associated with antibacterial resistance ...
genetic modification
... inserting them into another but this is not necessary to create a GMO. GMO can mean that an organism’s own genes are modified. ...
... inserting them into another but this is not necessary to create a GMO. GMO can mean that an organism’s own genes are modified. ...
lect 5- Cloning Vectors
... • Plasmids are self-replicating, double stranded, circular DNA molecules that are maintained in bacteria as independent extra chromosomal entities. • Virtually all bacterial genera have plasmids. ...
... • Plasmids are self-replicating, double stranded, circular DNA molecules that are maintained in bacteria as independent extra chromosomal entities. • Virtually all bacterial genera have plasmids. ...
F plasmid
... Plasmids: autonomously replicating extrachromosomal DNA molecules present mostly in the bacterial cells. Bacteriophages (phages): bacterial viruses. ...
... Plasmids: autonomously replicating extrachromosomal DNA molecules present mostly in the bacterial cells. Bacteriophages (phages): bacterial viruses. ...
INBREEDING Definition
... “An extra-chromosomal DNA that can replicate independently of the chromosomal DNA is called Plasmid”. History: J. Lederberg in 1952. Properties of Plasmid Show Resistance against the antibiotics, produce toxins. Examples 1. pSC 101 Tetracycline 2. pBR 322 Penicillin and Tetracycline Categories of Pl ...
... “An extra-chromosomal DNA that can replicate independently of the chromosomal DNA is called Plasmid”. History: J. Lederberg in 1952. Properties of Plasmid Show Resistance against the antibiotics, produce toxins. Examples 1. pSC 101 Tetracycline 2. pBR 322 Penicillin and Tetracycline Categories of Pl ...
Microbial Genetics - Montgomery College
... Vertical gene transfer: Occurs during reproduction between generations of cells. Horizontal gene transfer: The transfer of genes between cells of the same generation. Transformation Purpose of transformation? mechanism of transfer of genetic info among bacteria occurs naturally with linear ...
... Vertical gene transfer: Occurs during reproduction between generations of cells. Horizontal gene transfer: The transfer of genes between cells of the same generation. Transformation Purpose of transformation? mechanism of transfer of genetic info among bacteria occurs naturally with linear ...
Biotechnology Need To Know List
... How to recognize a diagram of DNA cut by a restriction enzyme What DNA analysis by gel electrophoresis allows researchers to do The technique used to make many copies of a gene What genetic engineering involves The technique of DNA sequencing How a recombinant plasmid gets inside a bacterial cell Wh ...
... How to recognize a diagram of DNA cut by a restriction enzyme What DNA analysis by gel electrophoresis allows researchers to do The technique used to make many copies of a gene What genetic engineering involves The technique of DNA sequencing How a recombinant plasmid gets inside a bacterial cell Wh ...
Concept Check Questions with answers
... blood cells. To obtain sufficient amounts of the protein, you decide to clone the β-globin gene. Would you construct a genomic library or a cDNA library? What material would you use as a source of DNA or RNA? ...
... blood cells. To obtain sufficient amounts of the protein, you decide to clone the β-globin gene. Would you construct a genomic library or a cDNA library? What material would you use as a source of DNA or RNA? ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.