Slide 1
... Development of Plasmid Vectors Plasmids serve as important tools in genetics and biochemistry labs, where they are commonly used to multiply or express particular genes. Plasmids used in genetic engineering are called vectors. Vectors are vehicles to transfer genes from one organism to another and ...
... Development of Plasmid Vectors Plasmids serve as important tools in genetics and biochemistry labs, where they are commonly used to multiply or express particular genes. Plasmids used in genetic engineering are called vectors. Vectors are vehicles to transfer genes from one organism to another and ...
Operons
... The cell is now considered a recombinant because its genome contains DNA from 2 different sources ...
... The cell is now considered a recombinant because its genome contains DNA from 2 different sources ...
lecture 2
... It is thought that approximately 50 global regulatory systems exist in a typical prokaryotic cell. C. Plasmids 1. Types of plasmids Sizes of plasmids can range from a few kilobase pairs to hundreds of kbp and can be linear or circular. Broad host range plasmids can replicate in diverse bacteria (enc ...
... It is thought that approximately 50 global regulatory systems exist in a typical prokaryotic cell. C. Plasmids 1. Types of plasmids Sizes of plasmids can range from a few kilobase pairs to hundreds of kbp and can be linear or circular. Broad host range plasmids can replicate in diverse bacteria (enc ...
Biotechnology II Recombinant DNA File
... fragments together (by utilizing “sticky ends”) This allows combinations of DNA segments from different organisms (due to universal base pairing rules) ...
... fragments together (by utilizing “sticky ends”) This allows combinations of DNA segments from different organisms (due to universal base pairing rules) ...
Transfection - Biomanufacturing.org
... for transfection in other cell types. Therefore they posses two different origins of replication suitable for both cell types. • Some origins of replications allow more efficient replications and yield high copy number of plasmids. • High copy number origins are preferred since more plasmids are rep ...
... for transfection in other cell types. Therefore they posses two different origins of replication suitable for both cell types. • Some origins of replications allow more efficient replications and yield high copy number of plasmids. • High copy number origins are preferred since more plasmids are rep ...
Cloning vectors share four common properties
... • The vector is chosen according to the size and type of DNA to be cloned ...
... • The vector is chosen according to the size and type of DNA to be cloned ...
pGLO Lab Write up – Jauss Biology 3 - Parkway C-2
... pGLO Lab Write up Part 1– Jauss Honors Biology 3 Do in lab notebook! Title: pGlo Lab Purpose: To transform E. coli bacteria by adding plasmids that allow the bacteria to glow green under UV light in the presence of arabinose sugar and grow in the presence of the antibiotic, ampicillin. Background: 1 ...
... pGLO Lab Write up Part 1– Jauss Honors Biology 3 Do in lab notebook! Title: pGlo Lab Purpose: To transform E. coli bacteria by adding plasmids that allow the bacteria to glow green under UV light in the presence of arabinose sugar and grow in the presence of the antibiotic, ampicillin. Background: 1 ...
Different Mechanisms for Turning On Viral Protein Production in
... in the laboratory. Different forms of the L1 gene (encoding the L1 protein) were inserted into circular DNA constructs called ´plasmids`. Human cells were infected with the plasmids to see how much L1 protein each different L1 gene produced, by using the cellular machinery in much the same way as th ...
... in the laboratory. Different forms of the L1 gene (encoding the L1 protein) were inserted into circular DNA constructs called ´plasmids`. Human cells were infected with the plasmids to see how much L1 protein each different L1 gene produced, by using the cellular machinery in much the same way as th ...
Bacterial Transformation
... Vehicles for foreign DNA Plasmid DNA usually contains genes for one or more traits that may be beneficial to bacterial survival Bacteria can transfer plasmids back and forth, allowing them to share beneficial genes Allows for adaptation to new environments Recent occurrence of bacterial antibiotic r ...
... Vehicles for foreign DNA Plasmid DNA usually contains genes for one or more traits that may be beneficial to bacterial survival Bacteria can transfer plasmids back and forth, allowing them to share beneficial genes Allows for adaptation to new environments Recent occurrence of bacterial antibiotic r ...
pTcGW platform guideline Gateway® cloning system: general
... reaction (PCR) amplification, then in the presence of BP Clonase mix, these sites recombine with attP1 and attP2 sites (BP reaction), found in the donor vector (e.g., pDONR 221). This will generate the attL1 and attL2 sites flanking the insert that at this stage is cloned in the donor vector (entry ...
... reaction (PCR) amplification, then in the presence of BP Clonase mix, these sites recombine with attP1 and attP2 sites (BP reaction), found in the donor vector (e.g., pDONR 221). This will generate the attL1 and attL2 sites flanking the insert that at this stage is cloned in the donor vector (entry ...
E. coli
... with the plasma cell membrane where they release DNA into the cell • Shuttle plasmids are plasmids engineered to infect eukaryotic cells. • A selectable marker (antibiotic resistance gene) such as neomycin and a promotor from a mammalian virus to aid in DNA insertion • CMV (cytomegalovirus) is a hum ...
... with the plasma cell membrane where they release DNA into the cell • Shuttle plasmids are plasmids engineered to infect eukaryotic cells. • A selectable marker (antibiotic resistance gene) such as neomycin and a promotor from a mammalian virus to aid in DNA insertion • CMV (cytomegalovirus) is a hum ...
13-3 Cell Transformation
... Bacterial plasmids can be used to transform plant cells. Agrobacterium tumefaciens Type of bacteria that inserts a plasmid into plant cells and grows tumors. The tumor-producing gene can be removed and replaced with recombinant DNA. If transformation is successful the recombinant DNA is in ...
... Bacterial plasmids can be used to transform plant cells. Agrobacterium tumefaciens Type of bacteria that inserts a plasmid into plant cells and grows tumors. The tumor-producing gene can be removed and replaced with recombinant DNA. If transformation is successful the recombinant DNA is in ...
Genetic Engineering Short Notes
... 1. Genetic engineering- remaking genes for practical purposes 2. Recombinant DNA- DNA made from two or more different organisms 3. Restriction enzyme- enzymes that recognize short specific DNA sequences and that cut the DNA there 4. Plasmid- small, circular DNA molecules that can replicate independa ...
... 1. Genetic engineering- remaking genes for practical purposes 2. Recombinant DNA- DNA made from two or more different organisms 3. Restriction enzyme- enzymes that recognize short specific DNA sequences and that cut the DNA there 4. Plasmid- small, circular DNA molecules that can replicate independa ...
The Wild World of Biotechnology!! Applications Genetic
... We use chemicals (CaCl2) and heat shock to get recombinant plasmids into the cell. We include antibiotic resistance genes in the recombinant plasmid so that only the successfully transformed bacteria live. We make sure the gene of interest is near a known operon and we intentionally turn that operon ...
... We use chemicals (CaCl2) and heat shock to get recombinant plasmids into the cell. We include antibiotic resistance genes in the recombinant plasmid so that only the successfully transformed bacteria live. We make sure the gene of interest is near a known operon and we intentionally turn that operon ...
Bacterial Mutation Analysis
... What we want to do… • Determine the rate of mutations in plasmids pBR325 CCTG 36 and pBR325 CCTG 58 in strains of E. coli MC4100 and Hfq-. • The mutations are the shrinking in length of a CCTG tract of 36 or 58 repeats. ...
... What we want to do… • Determine the rate of mutations in plasmids pBR325 CCTG 36 and pBR325 CCTG 58 in strains of E. coli MC4100 and Hfq-. • The mutations are the shrinking in length of a CCTG tract of 36 or 58 repeats. ...
Biotechnology Free Response Questions part II
... waste products. Bacteriophage (virus) were added at the time shown and the following population changes were observed. (a) Describe and explain the observed results. (b) Discuss the infection cycle of a DNA virus from attachment to lysis. (c) Describe how the genome of a retrovirus like HIV (Human I ...
... waste products. Bacteriophage (virus) were added at the time shown and the following population changes were observed. (a) Describe and explain the observed results. (b) Discuss the infection cycle of a DNA virus from attachment to lysis. (c) Describe how the genome of a retrovirus like HIV (Human I ...
BIO 220 Chapter 8 lecture outline Vocabulary Central dogma of
... 1. Be able to define all of the vocabulary used in lecture. 2. What is the central dogma of biology? Who proposed this theory? 3. What is the difference between the terms genotype and phenotype? Are bacteria typically diploid or haploid? What do diploid and haploid mean? 4. How many chromosomes does ...
... 1. Be able to define all of the vocabulary used in lecture. 2. What is the central dogma of biology? Who proposed this theory? 3. What is the difference between the terms genotype and phenotype? Are bacteria typically diploid or haploid? What do diploid and haploid mean? 4. How many chromosomes does ...
Biology 218 Microbial Metabolism and Genetics Chapter Six
... This experiment = part of the evidence that showed DNA was the genetic material. ...
... This experiment = part of the evidence that showed DNA was the genetic material. ...
A Bacterial Plasmid: What can you tell me about the plamid?
... organism’s DNA. Create sticky ends that are complementary to the plasmid’s sticky ends. • Insert the gene using ligase. How does one determine which RE’s to use? ...
... organism’s DNA. Create sticky ends that are complementary to the plasmid’s sticky ends. • Insert the gene using ligase. How does one determine which RE’s to use? ...
Assessment Questions Answer Key
... First, a restriction enzyme cuts both a bacterial plasmid and the human insulin gene. Then, an enzyme called ligase joins the nitrogen bases of the cut plasmid and human insulin gene together. This recreates a recombinant plasmid. Then this recombinant plasmid can be inserted into a bacterial cell. ...
... First, a restriction enzyme cuts both a bacterial plasmid and the human insulin gene. Then, an enzyme called ligase joins the nitrogen bases of the cut plasmid and human insulin gene together. This recreates a recombinant plasmid. Then this recombinant plasmid can be inserted into a bacterial cell. ...
Assessment Questions Answer Key
... First, a restriction enzyme cuts both a bacterial plasmid and the human insulin gene. Then, an enzyme called ligase joins the nitrogen bases of the cut plasmid and human insulin gene together. This recreates a recombinant plasmid. Then this recombinant plasmid can be inserted into a bacterial cell. ...
... First, a restriction enzyme cuts both a bacterial plasmid and the human insulin gene. Then, an enzyme called ligase joins the nitrogen bases of the cut plasmid and human insulin gene together. This recreates a recombinant plasmid. Then this recombinant plasmid can be inserted into a bacterial cell. ...
Slide 1 - Montville.net
... Take out the copied genes in plasmid from the bacteria. Take out the copied genes from the plasmids. Put the gene in another organism’s genomic DNA Reason #2 – Use to make a protein like a hormone. Gene in the plasmid can be turned on by the bacteria or yeast cell to make a protein. Extract the prot ...
... Take out the copied genes in plasmid from the bacteria. Take out the copied genes from the plasmids. Put the gene in another organism’s genomic DNA Reason #2 – Use to make a protein like a hormone. Gene in the plasmid can be turned on by the bacteria or yeast cell to make a protein. Extract the prot ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.