10. Genetic engineering and bacteria
... – Inserting gene for beta-carotene production into rice so that the molecule is present in the edible part of the rice plant. Beta-carotene can be converted into vitamin A in people who eat it. ...
... – Inserting gene for beta-carotene production into rice so that the molecule is present in the edible part of the rice plant. Beta-carotene can be converted into vitamin A in people who eat it. ...
Review Questions yeast lecture 18
... PCR of drug resistance cassette with primers that introduce 40-45bp of homology to the target gene on each end of the cassette. High efficiency transformation of yeast cells with the PCR product, selection for drug resistance. Confirmation of the knockout by PCR, using sets of primers where one olig ...
... PCR of drug resistance cassette with primers that introduce 40-45bp of homology to the target gene on each end of the cassette. High efficiency transformation of yeast cells with the PCR product, selection for drug resistance. Confirmation of the knockout by PCR, using sets of primers where one olig ...
Microbial Genetics
... – Generation of free radicals that can react with DNA – SS or DS breaks in the DNA molecule ...
... – Generation of free radicals that can react with DNA – SS or DS breaks in the DNA molecule ...
Cell Transformation Chapter 13-3
... B. Genetic Marker- can be a gene for antibiotic resistance that is spliced onto the gene that researchers want expressed. 1. Use a restriction enzyme to cut plasmid and use same enzyme to cut out segment of DNA 2. This creates ends with nucleotides that match “sticky ends” 3. Allows bacteria with th ...
... B. Genetic Marker- can be a gene for antibiotic resistance that is spliced onto the gene that researchers want expressed. 1. Use a restriction enzyme to cut plasmid and use same enzyme to cut out segment of DNA 2. This creates ends with nucleotides that match “sticky ends” 3. Allows bacteria with th ...
DNA plasmids/cloning
... Plasmids •Plasmid - an extrachromosomal circular DNA molecule that autonomously replicates (has an Ori ) inside the bacterial cell; cloning limit: 100 to 10,000 base pairs or 0.1-10 kilobases (kb) •10-100 copies per cell called high copy number •1-4 copies, low copy number •Many exist naturally in • ...
... Plasmids •Plasmid - an extrachromosomal circular DNA molecule that autonomously replicates (has an Ori ) inside the bacterial cell; cloning limit: 100 to 10,000 base pairs or 0.1-10 kilobases (kb) •10-100 copies per cell called high copy number •1-4 copies, low copy number •Many exist naturally in • ...
Vectors for Even Larger Genomic DNA Inserts
... Plasmid avoid being lost from dividing cells by carrying partitioning systems The functions involved in these systems are called par functions cis-acting site: parS trans-acting site: parA and parB ...
... Plasmid avoid being lost from dividing cells by carrying partitioning systems The functions involved in these systems are called par functions cis-acting site: parS trans-acting site: parA and parB ...
Horizontal Gene Transfer in Prokaryotes
... roles, including cycling elements in the soil, atmosphere and water. They present disease challenges to humans, animals and plants. Prokaryotes also play a central role as tools for biotechnology. In this lecture we will mainly focus on the group of the Bacteria. Slide 4 Prokaryotes usually reproduc ...
... roles, including cycling elements in the soil, atmosphere and water. They present disease challenges to humans, animals and plants. Prokaryotes also play a central role as tools for biotechnology. In this lecture we will mainly focus on the group of the Bacteria. Slide 4 Prokaryotes usually reproduc ...
S1 Methods.
... Plasmids. pEGFP-hASC and pmCherry-hASC plasmids were cloned by subcloning human ASC cDNA derived from pcDNA3-hASC plasmid into pEGFP-C3 (Clontech, USA) and pmCherry-C3.1 (in-house produced) vectors between HindIII and EcoRI sites. pcOVA-EYFP plasmid was cloned by subcloning of cytoplasmic ovalbumin ...
... Plasmids. pEGFP-hASC and pmCherry-hASC plasmids were cloned by subcloning human ASC cDNA derived from pcDNA3-hASC plasmid into pEGFP-C3 (Clontech, USA) and pmCherry-C3.1 (in-house produced) vectors between HindIII and EcoRI sites. pcOVA-EYFP plasmid was cloned by subcloning of cytoplasmic ovalbumin ...
first of four for Chapter 9
... R plasmids • Some conjugative plasmids in nature have accumulated different transposons containing multiple antibiotic-resistance genes (or transposons with integrons with the resistance genes). • These plasmids with multiple resistance genes are called R plasmids, and cause clinical trouble. ...
... R plasmids • Some conjugative plasmids in nature have accumulated different transposons containing multiple antibiotic-resistance genes (or transposons with integrons with the resistance genes). • These plasmids with multiple resistance genes are called R plasmids, and cause clinical trouble. ...
Cloning and selection
... When do the cutting and sticking of plasmid and foreign DNA there are several possible outcomes 1. Successful sticking of the plasmid and foreign DNA 2. Recircularization of plasmid without the foreign DNA 3. Circulization of plasmid with other plasmids or several inserts to make huge circular mol ...
... When do the cutting and sticking of plasmid and foreign DNA there are several possible outcomes 1. Successful sticking of the plasmid and foreign DNA 2. Recircularization of plasmid without the foreign DNA 3. Circulization of plasmid with other plasmids or several inserts to make huge circular mol ...
ppt
... The basic experimental techniques involved in gene cloning have now been described. A DNA molecule needs to display several features to be able to act as a vehicle for gene cloning. Most important, it must be able to replicate within the host cell, so that numerous copies of the recombinant DNA mole ...
... The basic experimental techniques involved in gene cloning have now been described. A DNA molecule needs to display several features to be able to act as a vehicle for gene cloning. Most important, it must be able to replicate within the host cell, so that numerous copies of the recombinant DNA mole ...
Cloning
... Why are plasmids suitable cloning vectors? Generally do not kill host cell Relatively easy to purify Can be made small ...
... Why are plasmids suitable cloning vectors? Generally do not kill host cell Relatively easy to purify Can be made small ...
chapter 19_updates
... DNA at specific nucleotide sequences • Type II restriction enzyme: most useful enzyme • By adding methyl groups to the recognition sequence to protect itself from being digested by its own enzyme in bacteria ...
... DNA at specific nucleotide sequences • Type II restriction enzyme: most useful enzyme • By adding methyl groups to the recognition sequence to protect itself from being digested by its own enzyme in bacteria ...
Recombinant DNA and Plasmids
... They help sickness with antibiotics (they carry antibiotics) Help make medicines to harmful bacteria Create antibiotics to combat bacteria Plasmids provide resistance to infections ...
... They help sickness with antibiotics (they carry antibiotics) Help make medicines to harmful bacteria Create antibiotics to combat bacteria Plasmids provide resistance to infections ...
GE Nova Video Questions
... Note: This video is 15 minutes in total. The answers to the worksheet are found between 6.55 minutes and 10 minutes approx. ...
... Note: This video is 15 minutes in total. The answers to the worksheet are found between 6.55 minutes and 10 minutes approx. ...
Plasmid
A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.