The Nature of Bacteria
... 5. Specialized transduction is limited to certain sites 6. Transduction is a source of genes for bacterial toxins ...
... 5. Specialized transduction is limited to certain sites 6. Transduction is a source of genes for bacterial toxins ...
Viruses as Pathogens in Bacterial Gene Regulation
... – replication via binary fission (splitting) – asexual process » offspring are clones ...
... – replication via binary fission (splitting) – asexual process » offspring are clones ...
USA Science and Engineering Festival Expo 2012
... Powerful molecular tools are now applied in wide-ranging aspects of biology - from curing diseases and significantly extending the average life span of human populations, to constructing and organizing the evolutionary ”Tree of Life”. You will use some molecular biological knowledge of DNA and use s ...
... Powerful molecular tools are now applied in wide-ranging aspects of biology - from curing diseases and significantly extending the average life span of human populations, to constructing and organizing the evolutionary ”Tree of Life”. You will use some molecular biological knowledge of DNA and use s ...
Slide 1 - Montville.net
... The objective of the Paper Plasmid lab is to have you create a paper recombinant plasmid, a plasmid with a new gene inserted. The plasmid will contain DNA from two different organisms. You will use colored paper, scissors and tape to do this. If you are successful, you will have a two colored paper ...
... The objective of the Paper Plasmid lab is to have you create a paper recombinant plasmid, a plasmid with a new gene inserted. The plasmid will contain DNA from two different organisms. You will use colored paper, scissors and tape to do this. If you are successful, you will have a two colored paper ...
Discussion Leader Activity: Recombinant DNA Technology
... restriction enzymes and do gel electrophoresis. Those with gels need to draw their plasmid based on the gel you gave them. Make your way around the room checking to see if any student is having a difficult time. After around 5+ minutes have the students start talking to neighbors trying to find thei ...
... restriction enzymes and do gel electrophoresis. Those with gels need to draw their plasmid based on the gel you gave them. Make your way around the room checking to see if any student is having a difficult time. After around 5+ minutes have the students start talking to neighbors trying to find thei ...
GENETIC TRANSFER AND RECOMBINATION (Chapter 8):
... Vertical gene transfer: between parent and offspring Horizontal gene transfer: between other organisms in the same generation Three types: 1. Transformation 2. Conjugation 3. Transduction All types: Involve unidirectional transfer of information (donor to recipient—recipient called recombinant cell) ...
... Vertical gene transfer: between parent and offspring Horizontal gene transfer: between other organisms in the same generation Three types: 1. Transformation 2. Conjugation 3. Transduction All types: Involve unidirectional transfer of information (donor to recipient—recipient called recombinant cell) ...
Bacteria and Recombinant DNA
... Fertility-(F-)plasmids, which contain only tra-genes. Their only function is to initiate conjugation. Resistance-(R-)plasmids, which contain genes that can build a resistance against antibiotics or poisons. Col-plasmids, which contain genes that code for (determine the production of) colicines, prot ...
... Fertility-(F-)plasmids, which contain only tra-genes. Their only function is to initiate conjugation. Resistance-(R-)plasmids, which contain genes that can build a resistance against antibiotics or poisons. Col-plasmids, which contain genes that code for (determine the production of) colicines, prot ...
Activity #2 Teacher - Center for Occupational Research and
... Now that you have examined the plasmids, choose which plasmid you would use considering the following parameters: Restriction enzymes can be expensive; therefore, it is often a good idea to use an enzyme that you or colleagues already have in the lab. The same is true for antibiotics. In your lab y ...
... Now that you have examined the plasmids, choose which plasmid you would use considering the following parameters: Restriction enzymes can be expensive; therefore, it is often a good idea to use an enzyme that you or colleagues already have in the lab. The same is true for antibiotics. In your lab y ...
Chapter 12 - gontarekapbio
... Note: the plasmid vector usually also contains an antibiotic resistance gene that will allow scientists to isolate colonies that have the GOI. (Will grow bacteria on pates w/antibiotic – those with out the plasmid will be killed, those with plasmid can survive). ...
... Note: the plasmid vector usually also contains an antibiotic resistance gene that will allow scientists to isolate colonies that have the GOI. (Will grow bacteria on pates w/antibiotic – those with out the plasmid will be killed, those with plasmid can survive). ...
Antibiotic resistance genes are carried on plasmids
... transfer a copy of the R-plasmids to other bacteria, making them also multiple antibiotic resistant and able to produce a conjugation pilus. In addition, some exotoxins , such as the tetanus exotoxin and Escherichia coli enterotoxin discussed later in this unit under Bacterial Pathogenicity, are als ...
... transfer a copy of the R-plasmids to other bacteria, making them also multiple antibiotic resistant and able to produce a conjugation pilus. In addition, some exotoxins , such as the tetanus exotoxin and Escherichia coli enterotoxin discussed later in this unit under Bacterial Pathogenicity, are als ...
ConjugationSlides
... Bacterial Conjugation • Conjugation will be used as a means to transfer genetic information from one bacteria to another • Cells with a F+ plasmid or a R plasmid can transfer their DNA to other bacteria • On the F and R plasmids, DNA transfer begins at a specific sequence, OriT, the Origin of Transf ...
... Bacterial Conjugation • Conjugation will be used as a means to transfer genetic information from one bacteria to another • Cells with a F+ plasmid or a R plasmid can transfer their DNA to other bacteria • On the F and R plasmids, DNA transfer begins at a specific sequence, OriT, the Origin of Transf ...
Transformation Pre-Lab
... 5. Give several examples of bacteria that transform naturally, AND explain how they go through this process. 6. How would these bacteria select for DNA that is likely to be beneficial for them? 7. Give an example of how it would be beneficial for a bacterium to bring in and use DNA from other member ...
... 5. Give several examples of bacteria that transform naturally, AND explain how they go through this process. 6. How would these bacteria select for DNA that is likely to be beneficial for them? 7. Give an example of how it would be beneficial for a bacterium to bring in and use DNA from other member ...
recombinant dna and polymerase chain reactions
... strands of DNA can be separated and re-associated by heating and cooling One strand of DNA specifies the sequence of the other strand ...
... strands of DNA can be separated and re-associated by heating and cooling One strand of DNA specifies the sequence of the other strand ...
Chapter 9 Biotechnology
... • Extract the restriction enzyme from bacteria and use it genetic engineering • EcoRI, BamHI – recognize specific sequence • Staggered cuts on DNA • Ends of the fragment are single stranded ...
... • Extract the restriction enzyme from bacteria and use it genetic engineering • EcoRI, BamHI – recognize specific sequence • Staggered cuts on DNA • Ends of the fragment are single stranded ...
Genetic Engineering ppt
... This colony will probably have the correct plasmid to produce the product from the human gene. Cells from this colony will be grown on a large scale and the medium analysed for the presence of the product from the human gene, eg growth hormone ...
... This colony will probably have the correct plasmid to produce the product from the human gene. Cells from this colony will be grown on a large scale and the medium analysed for the presence of the product from the human gene, eg growth hormone ...
Ligation and Transformation
... Overall Transformation Process 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection ...
... Overall Transformation Process 1. The plasmid vector must be cut with a restriction endonuclease (aka: restriction enzyme) 2. DNA ligase joins the DNA fragment & vector DNA 3. Host cell is made competent so can plasmid can enter 4. Transformed cells are grown on selection ...
Recombinant DNA and Gene Cloning
... Plasmids are replicated by the same machinery that replicates the bacterial chromosome. Some plasmids are copied at about the same rate as the chromosome, so a single cell is apt to have only a single copy of the plasmid. Other plasmids are copied at a high rate and a single cell may have 50 or more ...
... Plasmids are replicated by the same machinery that replicates the bacterial chromosome. Some plasmids are copied at about the same rate as the chromosome, so a single cell is apt to have only a single copy of the plasmid. Other plasmids are copied at a high rate and a single cell may have 50 or more ...
Bacteria Worksheet #3
... 1. Compare and contrast between sexual and asexual reproduction in bacteria. ...
... 1. Compare and contrast between sexual and asexual reproduction in bacteria. ...
DNA-Mediated Transformation
... • bacteriophages – have the natural ability to inject their DNA into bacterial hosts through transduction ...
... • bacteriophages – have the natural ability to inject their DNA into bacterial hosts through transduction ...
doc BIOL200 quiz 4 afternoon
... Question options: The only way to detect the presence of a plasmid in bacteria is to screen by ...
... Question options: The only way to detect the presence of a plasmid in bacteria is to screen by ...
What is Willy Wonka famous for?
... he’s working with is genetic engineering. • Goal: Learn how genetic engineering works and apply the technology to other situations. ...
... he’s working with is genetic engineering. • Goal: Learn how genetic engineering works and apply the technology to other situations. ...
Recombinant DNA and Gene Cloning
... ruptured to release its DNA. The tangle is a about the same rate as the chromosome, portion of a single DNA molecule containing so a single cell is apt to have only a single over 4.6 million base pairs encoding approximately 4,300 genes. The small copy of the plasmid. Other plasmids are circlets are ...
... ruptured to release its DNA. The tangle is a about the same rate as the chromosome, portion of a single DNA molecule containing so a single cell is apt to have only a single over 4.6 million base pairs encoding approximately 4,300 genes. The small copy of the plasmid. Other plasmids are circlets are ...
DNA-Mediated Transformation
... Explored by F. Griffith (1928) using pneumococci The DNA released from a degenerated bacterium enters into another bacterium. There is no vector or mediation in this type of gene exchange. ...
... Explored by F. Griffith (1928) using pneumococci The DNA released from a degenerated bacterium enters into another bacterium. There is no vector or mediation in this type of gene exchange. ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.