Genetic Engineering
... Genomic Libraries – (total DNA of a cell is referred to as a genome) • genomic library – a collection of DNA fragments that are more or less representative of all the DNA in the genome • each fragment is spliced into a plasmid, which is usually contained inside a bacterial cell • restriction enzym ...
... Genomic Libraries – (total DNA of a cell is referred to as a genome) • genomic library – a collection of DNA fragments that are more or less representative of all the DNA in the genome • each fragment is spliced into a plasmid, which is usually contained inside a bacterial cell • restriction enzym ...
Drug resistance of bacteria commensal with Drosophila
... glycosidic bonds (50 µg/ml in food). Antibiotics were added as liquid food cooled below 50°C, from stock solutions in ethanol (o-phenyl phenol), methanol (tetracycline), or water (ceftriaxone, spectinomycin). The principal was to follow sharp reduction in the bacterial load with simultaneous exposu ...
... glycosidic bonds (50 µg/ml in food). Antibiotics were added as liquid food cooled below 50°C, from stock solutions in ethanol (o-phenyl phenol), methanol (tetracycline), or water (ceftriaxone, spectinomycin). The principal was to follow sharp reduction in the bacterial load with simultaneous exposu ...
Genetics of Viruses and Bacteria
... Conjugation • Direct transfer of genetic material (usually plasmid DNA) from two bacterial cells that are temporarily joined by a sex pili. • Plasmid genes are not required for survival, but they tend to code for genes that increase fitness (ex. antibiotic resistance) video ...
... Conjugation • Direct transfer of genetic material (usually plasmid DNA) from two bacterial cells that are temporarily joined by a sex pili. • Plasmid genes are not required for survival, but they tend to code for genes that increase fitness (ex. antibiotic resistance) video ...
013368718X_CH15_229-246.indd
... 18. An organism that contains one or more genes from another species is inbred. 19. Transgenic organisms can be made by inserting recombinant DNA into the genome of the host organism. 20. Examining the properties of a transgenic organism allows scientists to discover the function of the transferred ...
... 18. An organism that contains one or more genes from another species is inbred. 19. Transgenic organisms can be made by inserting recombinant DNA into the genome of the host organism. 20. Examining the properties of a transgenic organism allows scientists to discover the function of the transferred ...
Big, strong, fast, and aggressive
... A strain of bacteria has been developed that can digest oil to help clean oil spills Scientists are trying to produce bacteria to clean up radioactive waste ...
... A strain of bacteria has been developed that can digest oil to help clean oil spills Scientists are trying to produce bacteria to clean up radioactive waste ...
Name Date Period BioTechnology: Web Quest Part 1
... 4. What charge does the DNA fragments have? ____________________. 5. If you place the DNA on the negative side of the gel box it will be attracted to the ….? __________________________________________________________________ _______ 6. What do you think happens if the DNA fragments are all different ...
... 4. What charge does the DNA fragments have? ____________________. 5. If you place the DNA on the negative side of the gel box it will be attracted to the ….? __________________________________________________________________ _______ 6. What do you think happens if the DNA fragments are all different ...
No Slide Title
... is thought to be caused by horizontal gene transfer and also inversions around the origin of replication ...
... is thought to be caused by horizontal gene transfer and also inversions around the origin of replication ...
Today`s Plan: 4/25/03
... • DNA can be manipulated to contain certain genes. Manipulations are performed by cutting DNA, inserting what we want, and inserting the DNA back into a cell. • A manipulated piece of DNA, that contains “spliced” pieces is called recombinant DNA • The recombinant DNA comes from cloning vectors-carri ...
... • DNA can be manipulated to contain certain genes. Manipulations are performed by cutting DNA, inserting what we want, and inserting the DNA back into a cell. • A manipulated piece of DNA, that contains “spliced” pieces is called recombinant DNA • The recombinant DNA comes from cloning vectors-carri ...
Slide 1
... Answer: D a. Bacterial proteins transferred from the donor bacterium by the phage to the recipient bacterium recombine with genes on the recipient’s chromosome. b. The recipient bacterium incorporates the transduced genetic material coding for phage proteins into its chromosome and synthesizes the c ...
... Answer: D a. Bacterial proteins transferred from the donor bacterium by the phage to the recipient bacterium recombine with genes on the recipient’s chromosome. b. The recipient bacterium incorporates the transduced genetic material coding for phage proteins into its chromosome and synthesizes the c ...
Biology: Unit 13 Directed Reading Guide
... ______ Why must a genetically engineered plasmid contain a genetic marker? e. to prevent the construction of an artificial chromosome f. to separate cells that contain recombinant DNA from those that do not g. to produce multiple copies of the recombined plasmid after heat treatment h. to break apar ...
... ______ Why must a genetically engineered plasmid contain a genetic marker? e. to prevent the construction of an artificial chromosome f. to separate cells that contain recombinant DNA from those that do not g. to produce multiple copies of the recombined plasmid after heat treatment h. to break apar ...
7.014 Quiz III Handout
... a) Autosomal dominant, because affected individuals can be of either sex, occur in all generations, and always have an affected parent. If d = normal allele and D = affected rare ...
... a) Autosomal dominant, because affected individuals can be of either sex, occur in all generations, and always have an affected parent. If d = normal allele and D = affected rare ...
Supporting Information
... (human lung adenocarcinoma cell line), T24 (human urinary bladder carcinoma cell line), NHLF-1 (human normal lung fibroblasts cell line), MRC-5 (human embryonic fibroblasts cell line) and BEAS-2B (human normal bronchial epithelial cell line) cells were purchased from ATCC (Rockville, MD); BEL-7404 ( ...
... (human lung adenocarcinoma cell line), T24 (human urinary bladder carcinoma cell line), NHLF-1 (human normal lung fibroblasts cell line), MRC-5 (human embryonic fibroblasts cell line) and BEAS-2B (human normal bronchial epithelial cell line) cells were purchased from ATCC (Rockville, MD); BEL-7404 ( ...
Genetic Technology
... Do this with 2 sheets of paper. Label the sides of one paper: inbreeding, Clone, genetic engineering, plasmid, recombinant DNA, restriction enzyme. • Label the side of the other paper: transgenic organism, vector, gene therapy, human genome, PCR ...
... Do this with 2 sheets of paper. Label the sides of one paper: inbreeding, Clone, genetic engineering, plasmid, recombinant DNA, restriction enzyme. • Label the side of the other paper: transgenic organism, vector, gene therapy, human genome, PCR ...
Bacteria and Viruses Bacterial Cells Bacterial Genome Bacterial
... – Contagious disease pathogens must directly contact a new host. They may cause… • Epidemics (incidence rate exceeds expected number), pandemics (worldwide epidemic), sporadic (“singular”) cases, and endemic (maintained in a population) cases ...
... – Contagious disease pathogens must directly contact a new host. They may cause… • Epidemics (incidence rate exceeds expected number), pandemics (worldwide epidemic), sporadic (“singular”) cases, and endemic (maintained in a population) cases ...
Supplementary Materials: Immobilization of Genetically
... (VPGXG)60, and Xaa was chosen to be Val:Ala:Gly in a 5:3:2 ratio [1]. The gene sequence of the monomer (VPGXG)20 is listed in Table S1. PUC57‐(VPGXG)20 was linearized with PflMI (2 μL PflM I; 3 μL 10 × K buffer; 25 μL PUC57‐ (VPGXG)20 at 37 °C for 3 h), enzymatically dephosphoryla ...
... (VPGXG)60, and Xaa was chosen to be Val:Ala:Gly in a 5:3:2 ratio [1]. The gene sequence of the monomer (VPGXG)20 is listed in Table S1. PUC57‐(VPGXG)20 was linearized with PflMI (2 μL PflM I; 3 μL 10 × K buffer; 25 μL PUC57‐ (VPGXG)20 at 37 °C for 3 h), enzymatically dephosphoryla ...
Genetic Engineering
... DNA is in cytoplasm Plasmids are small circular Extra DNA… We use Plasmids because they are small and easy to manipulate (Called “Vectors”) • Plus, Bacteria replicate quickly! ...
... DNA is in cytoplasm Plasmids are small circular Extra DNA… We use Plasmids because they are small and easy to manipulate (Called “Vectors”) • Plus, Bacteria replicate quickly! ...
Producing a Recombinant Plasmid, pARA-R
... Producing a Recombinant Plasmid, pARA-R during Lab 2 will be ligated, or bonded together, using DNA ligase, making new recombinant plasmids. These newly formed plasmids will represent recombinant DNA molecules because the four restriction fragments have been recombined in different ways to produce n ...
... Producing a Recombinant Plasmid, pARA-R during Lab 2 will be ligated, or bonded together, using DNA ligase, making new recombinant plasmids. These newly formed plasmids will represent recombinant DNA molecules because the four restriction fragments have been recombined in different ways to produce n ...
Recovery and analysis of old/ancient DNA: molecular archaeology
... polyploid species of Chrysanthemum ...
... polyploid species of Chrysanthemum ...
Chapter 9 Genetics Chromosome Genes • DNA RNA Protein Flow of
... undergo a lytic cycle and are capable of transducing any part of the donor’s genetic information THIS IS A RANDOM EVENT ...
... undergo a lytic cycle and are capable of transducing any part of the donor’s genetic information THIS IS A RANDOM EVENT ...
Fact Sheet Describing Recombinant DNA and Elements
... Introduce the DNA into bacterial cells or yeast cells by transformation. ...
... Introduce the DNA into bacterial cells or yeast cells by transformation. ...
Biologists have learned to manipulate DNA
... A. Human genome- map of all humans genes was completed by 2000 1. Other organisms sequenced: fruit fly, yeast, E. coli, or rice plant B. Uses 1. Improve food nutrition 2. Help us understand how our genes work from others 13.2 Biologists can engineer bacteria to make useful products I. Engineering ba ...
... A. Human genome- map of all humans genes was completed by 2000 1. Other organisms sequenced: fruit fly, yeast, E. coli, or rice plant B. Uses 1. Improve food nutrition 2. Help us understand how our genes work from others 13.2 Biologists can engineer bacteria to make useful products I. Engineering ba ...
Bacterial recombination
... F factor (plasmid) carries DNA from “male” to “female” bacterium F factor ...
... F factor (plasmid) carries DNA from “male” to “female” bacterium F factor ...
PLTW Biomedical Competency Profile
... Isolates DNA from cells Separates DNA fragments by gel electrophoresis Analyzes gel electrophoresis results Uses aseptic technique to sample and transfer bacterial cells Uses proper Gram staining techniques to stain and observe bacteria Performs and analyzes antibiotic efficiency testing Analyzes bo ...
... Isolates DNA from cells Separates DNA fragments by gel electrophoresis Analyzes gel electrophoresis results Uses aseptic technique to sample and transfer bacterial cells Uses proper Gram staining techniques to stain and observe bacteria Performs and analyzes antibiotic efficiency testing Analyzes bo ...
Table II Transformation of various derived strains OSU Strain Outcrossed with
... 1984 Neurospora Newsl. 31: 32). Each E. coli clone of the library contains recombinant DNA molecules i.e. fragments of Neursopora DNA inserted into the BamHI site of the cosmid vector. DNA was prepared from all 5000 clones of the library (a recombinant cosmid-pool) and it was purified further on a C ...
... 1984 Neurospora Newsl. 31: 32). Each E. coli clone of the library contains recombinant DNA molecules i.e. fragments of Neursopora DNA inserted into the BamHI site of the cosmid vector. DNA was prepared from all 5000 clones of the library (a recombinant cosmid-pool) and it was purified further on a C ...
Learning objectives
... 1. Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 2. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. 3. Outline the procedures for cloning a eukaryotic gene in a bacte ...
... 1. Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 2. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. 3. Outline the procedures for cloning a eukaryotic gene in a bacte ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.