Cloning in bacteria other than Escherichia coli
... used. The reasons for this are threefold. First, it is relatively simple to do, particularly now that competent cells are commercially available. Secondly, it can be very efficient. Efficiencies of 108–109 transformants/ µg plasmid DNA are readily achievable and are more than adequate for most appli ...
... used. The reasons for this are threefold. First, it is relatively simple to do, particularly now that competent cells are commercially available. Secondly, it can be very efficient. Efficiencies of 108–109 transformants/ µg plasmid DNA are readily achievable and are more than adequate for most appli ...
Restriction Enzymes
... several different methods all involving perturbing the bacterial membrane: Tbl 6.1 ...
... several different methods all involving perturbing the bacterial membrane: Tbl 6.1 ...
Chapter 13 DNA Technology
... Genomic Library – The set of 1000’s of DNA pieces from a genome produced from the use of restriction enzymes. Several libraries can be made from the same genome, depending on types of restriction enzymes used. Some of the DNA pieces will contain specific genes that can be transferred, if desired. Re ...
... Genomic Library – The set of 1000’s of DNA pieces from a genome produced from the use of restriction enzymes. Several libraries can be made from the same genome, depending on types of restriction enzymes used. Some of the DNA pieces will contain specific genes that can be transferred, if desired. Re ...
DNA Technology - 2 What are plasmids?
... material from the 400 year old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds. The most useful method for increasing the amount of dodo DNA available for testing is __________ 5. Why is golden rice pale yellow in color? a It is rich in chl ...
... material from the 400 year old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds. The most useful method for increasing the amount of dodo DNA available for testing is __________ 5. Why is golden rice pale yellow in color? a It is rich in chl ...
Prokaryotes, Viruses, and Protistans
... • Bacteria are the smallest living organisms • Viruses are smaller but are not alive ...
... • Bacteria are the smallest living organisms • Viruses are smaller but are not alive ...
Biology Test Chapters 13 Name and Honor Code: 1. The insertion of
... c. vectors d. transgenic organisms 6. The process by which desired traits of certain plants & animals are selected and passed on to their future generations: a. karyotype b. selective breeding c. human genome d. gene therapy 7. Bacterial proteins that have the ability to cut both strands of the DNA ...
... c. vectors d. transgenic organisms 6. The process by which desired traits of certain plants & animals are selected and passed on to their future generations: a. karyotype b. selective breeding c. human genome d. gene therapy 7. Bacterial proteins that have the ability to cut both strands of the DNA ...
File
... • Wild-type λ DNA contains several target sites for most of the commonly used restriction endonucleases and so is not itself suitable as a vector. • Derivatives of the wild-type phage have therefore been produced that either have a single target site at which foreign DNA can be inserted (insertional ...
... • Wild-type λ DNA contains several target sites for most of the commonly used restriction endonucleases and so is not itself suitable as a vector. • Derivatives of the wild-type phage have therefore been produced that either have a single target site at which foreign DNA can be inserted (insertional ...
09/06
... linear and have sticky ends (in this case). By complementary base pairing, the sticky ends can hybridize. The result is a recombinant DNA molecule. ...
... linear and have sticky ends (in this case). By complementary base pairing, the sticky ends can hybridize. The result is a recombinant DNA molecule. ...
Virus - DavidThompsonMercy
... transfer of genetic material between two bacterial cells. Bacterial "sex”. Used to map genetic sequences in bacteria. ...
... transfer of genetic material between two bacterial cells. Bacterial "sex”. Used to map genetic sequences in bacteria. ...
Class details
... How does Dr. K “look” at figures? Figure out the experimental rationale, design Make predictions Look at figures – what are the results? Predictions VS Results -> Do I believe it? Missing information? Data? Controls? Each individual figure <-> Main purpose ...
... How does Dr. K “look” at figures? Figure out the experimental rationale, design Make predictions Look at figures – what are the results? Predictions VS Results -> Do I believe it? Missing information? Data? Controls? Each individual figure <-> Main purpose ...
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.
... Three classes of recombinants between plasmids and chromosomes: Strain DBY93 1 (ura3-50 leu2) was transformed with plasmid pRB3O or pRB29 (both plasmids carry ura3-3; see Table 3) selecting Leu'. Independent Ura+ recombinants that arose were selected and subcloned, maintaining selection for the Ura' ...
... Three classes of recombinants between plasmids and chromosomes: Strain DBY93 1 (ura3-50 leu2) was transformed with plasmid pRB3O or pRB29 (both plasmids carry ura3-3; see Table 3) selecting Leu'. Independent Ura+ recombinants that arose were selected and subcloned, maintaining selection for the Ura' ...
Genetics of Viruses and Bacteria
... Conjugation • Direct transfer of genetic material (usually plasmid DNA) from two bacterial cells that are temporarily joined by a sex pili. • Plasmid genes are not required for survival, but they tend to code for genes that increase fitness (ex. antibiotic resistance) video ...
... Conjugation • Direct transfer of genetic material (usually plasmid DNA) from two bacterial cells that are temporarily joined by a sex pili. • Plasmid genes are not required for survival, but they tend to code for genes that increase fitness (ex. antibiotic resistance) video ...
Zinc finger nucleases
... of targetedcellswillcontain the desired genetic modification, a frequency too low to be useful for gene therapy[14]. However, proof‐of‐principle experiments involving the meganuclease I‐SceI, which binds to an 18‐bp recognition site, demonstrated that the insertion of a DSB in the target locus stimu ...
... of targetedcellswillcontain the desired genetic modification, a frequency too low to be useful for gene therapy[14]. However, proof‐of‐principle experiments involving the meganuclease I‐SceI, which binds to an 18‐bp recognition site, demonstrated that the insertion of a DSB in the target locus stimu ...
Journal of Biotechnology 84:
... a metabolic load in the host microorganism (Glick, 1995b). In this study, the growth of transconjugants with increased mps activity was evaluated in NB medium, in comparison with the strains without the recombinant plasmid. The growth rate and final cell density of the transconjugants were similar t ...
... a metabolic load in the host microorganism (Glick, 1995b). In this study, the growth of transconjugants with increased mps activity was evaluated in NB medium, in comparison with the strains without the recombinant plasmid. The growth rate and final cell density of the transconjugants were similar t ...
Supplementary Methods
... his5+ genes was amplified from the plasmid pFA6a-link-yEGFP-SpHIS532, and the product was integrated into the CRN1 locus in yeast by homologous recombination and selection on SD–His plates; in-frame fusion of the GFP moiety after codon 400 of CRN1 was confirmed by PCR. Using genomic DNA from this s ...
... his5+ genes was amplified from the plasmid pFA6a-link-yEGFP-SpHIS532, and the product was integrated into the CRN1 locus in yeast by homologous recombination and selection on SD–His plates; in-frame fusion of the GFP moiety after codon 400 of CRN1 was confirmed by PCR. Using genomic DNA from this s ...
slides
... • Bacterial genomes are streamlined, genes are closely packed with relatively little spacer DNA and few introns • Mammalian genome contains enormous amounts of nongene DNA including introns, spacer DNA between genes and various types of repetitive DNA sequences with similarities to transposons. Also ...
... • Bacterial genomes are streamlined, genes are closely packed with relatively little spacer DNA and few introns • Mammalian genome contains enormous amounts of nongene DNA including introns, spacer DNA between genes and various types of repetitive DNA sequences with similarities to transposons. Also ...
Chapter 13 - Angelfire
... • Called sticky ends because they want to bond with things due to their “open” end ...
... • Called sticky ends because they want to bond with things due to their “open” end ...
Recombinant DNA Technology
... genetics Nonpathogenic Can process mRNA and proteins Easy to grow ...
... genetics Nonpathogenic Can process mRNA and proteins Easy to grow ...
DNA cloning
... lac l, basically IPTG is not needed. Therefore, DH5α allows easy selection of recombinant DNA with X-Gal when constructing gene library or subcloning recombinant plasmid. ...
... lac l, basically IPTG is not needed. Therefore, DH5α allows easy selection of recombinant DNA with X-Gal when constructing gene library or subcloning recombinant plasmid. ...
Simulation of Gene Splicing (Genetic Engineering
... Terry's biology class had been studying human inheritance and were now discussing genetic defects. When Terry heard about dwarfs and midgets, he began to think about his sister, Julie, who had always been shorter than normal for her age. He remembered the concern of his parents when Julie was younge ...
... Terry's biology class had been studying human inheritance and were now discussing genetic defects. When Terry heard about dwarfs and midgets, he began to think about his sister, Julie, who had always been shorter than normal for her age. He remembered the concern of his parents when Julie was younge ...
Recombinant and Synthetic Nucleic Acid Activity Registration
... Consist entirely of DNA segments from a single nonchromosomal or viral DNA source, though one or more of the segments may be a synthetic equivalent. Consist entirely of DNA from a prokaryotic host including its indigenous plasmids or viruses when propagated only in that host (or a closely related st ...
... Consist entirely of DNA segments from a single nonchromosomal or viral DNA source, though one or more of the segments may be a synthetic equivalent. Consist entirely of DNA from a prokaryotic host including its indigenous plasmids or viruses when propagated only in that host (or a closely related st ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.