Recombinant DNA and Genetic Engineering
... Golden Rice or Frankenfood? • Scientists transferred daffodil genes into rice • Rice with beta-carotene may help prevent vitamin A deficiencies • Opponents fear unforeseen consequences of creating genetically modified organisms ...
... Golden Rice or Frankenfood? • Scientists transferred daffodil genes into rice • Rice with beta-carotene may help prevent vitamin A deficiencies • Opponents fear unforeseen consequences of creating genetically modified organisms ...
Genetic Engineering
... Steps for Plasmid Use 1. Get the DNA for the trait 2. Insert DNA into the plasmid 3. Bacterial transformation 4. Identification of the new trait *Fig 20.4, page 399 ...
... Steps for Plasmid Use 1. Get the DNA for the trait 2. Insert DNA into the plasmid 3. Bacterial transformation 4. Identification of the new trait *Fig 20.4, page 399 ...
LAB EXERCISE: Genetic Transformation
... and glow in the dark. Following the transformation procedure, the bacteria express their newly acquired jellyfish gene and produce the fluorescent protein, which causes them to glow a brilliant green color under ultraviolet light. In addition to one large chromosome, bacteria naturally contain one o ...
... and glow in the dark. Following the transformation procedure, the bacteria express their newly acquired jellyfish gene and produce the fluorescent protein, which causes them to glow a brilliant green color under ultraviolet light. In addition to one large chromosome, bacteria naturally contain one o ...
Lesson 2 Transformation Laboratory
... and glow in the dark. Following the transformation procedure, the bacteria express their newly acquired jellyfish gene and produce the fluorescent protein, which causes them to glow a brilliant green color under ultraviolet light. In addition to one large chromosome, bacteria naturally contain one o ...
... and glow in the dark. Following the transformation procedure, the bacteria express their newly acquired jellyfish gene and produce the fluorescent protein, which causes them to glow a brilliant green color under ultraviolet light. In addition to one large chromosome, bacteria naturally contain one o ...
Name_______________________ Period___________ Chapter
... What are genetically modified organisms, and why are they controversial? An organism that has acquired one or more genes by artificial means; also known as a transgenic organism. Advocates of cautious approach toward GM crops fear that transgenic plants might pass their new genes to close relatives ...
... What are genetically modified organisms, and why are they controversial? An organism that has acquired one or more genes by artificial means; also known as a transgenic organism. Advocates of cautious approach toward GM crops fear that transgenic plants might pass their new genes to close relatives ...
Chapter 20: Biotechnology - Staff Web Sites @ BBHCSD
... What are genetically modified organisms, and why are they controversial? An organism that has acquired one or more genes by artificial means; also known as a transgenic organism. Advocates of cautious approach toward GM crops fear that transgenic plants might pass their new genes to close relatives ...
... What are genetically modified organisms, and why are they controversial? An organism that has acquired one or more genes by artificial means; also known as a transgenic organism. Advocates of cautious approach toward GM crops fear that transgenic plants might pass their new genes to close relatives ...
10529_2013_1416_MOESM1_ESM
... containing 50 μg mL-1 apramycin, 25 μg mL-1 nalidixic acid for 5 days to confirm resistance. To eliminate autonomously replicating plasmid pTG2003, the exconjugants were incubated in YEME culture containing 20 µg mL-1 apramycin, 25 μg mL-1 nalidixic acid for 3 days. The strains of exconjugants were ...
... containing 50 μg mL-1 apramycin, 25 μg mL-1 nalidixic acid for 5 days to confirm resistance. To eliminate autonomously replicating plasmid pTG2003, the exconjugants were incubated in YEME culture containing 20 µg mL-1 apramycin, 25 μg mL-1 nalidixic acid for 3 days. The strains of exconjugants were ...
Gene Cloning
... □ Transformation of ligated product into E. coli cells □ Look up information on culturing bacteria and create a presentation on it □ Pre-lab cell culturing portion of the lab ...
... □ Transformation of ligated product into E. coli cells □ Look up information on culturing bacteria and create a presentation on it □ Pre-lab cell culturing portion of the lab ...
Restriction mapping
... The discovery of restriction enzymes, or restriction endonucleases (REs), was pivotal to the development of molecular cloning. REs occur naturally in bacteria, where they specifically recognize short stretches of nucleotides in DNA and catalyze double-strand breaks at or near the recognition site (a ...
... The discovery of restriction enzymes, or restriction endonucleases (REs), was pivotal to the development of molecular cloning. REs occur naturally in bacteria, where they specifically recognize short stretches of nucleotides in DNA and catalyze double-strand breaks at or near the recognition site (a ...
BIO113H - willisworldbio
... A ________ marker is a gene that makes it possible to distinguish bacteria that carry the plasmid (and the foreign DNA) from those that don’t. These markers allows us to distinguish that plasmid from other cells that does not have the __________ DNA. After transformation the cells are treated with ...
... A ________ marker is a gene that makes it possible to distinguish bacteria that carry the plasmid (and the foreign DNA) from those that don’t. These markers allows us to distinguish that plasmid from other cells that does not have the __________ DNA. After transformation the cells are treated with ...
The Effects of varying pH on Plasmid Transfer
... an example of an episome. The F plasmid is not only conjugative but it can also integrate itself into the recipient’s chromosome. Strains of bacteria that possess an integrated F plasmid are called Hfr, which stands for high frequency of recombination. This allows the recipient’s chromosome to be co ...
... an example of an episome. The F plasmid is not only conjugative but it can also integrate itself into the recipient’s chromosome. Strains of bacteria that possess an integrated F plasmid are called Hfr, which stands for high frequency of recombination. This allows the recipient’s chromosome to be co ...
DNA CLONING
... Do not kill its bacterial host: About 1000 phage particles are produced per cell generation and released into the medium Formas plaques on a lawn of bacteria which is due to the slower growth of bacteria wher infected by the phage, not cell lysis ...
... Do not kill its bacterial host: About 1000 phage particles are produced per cell generation and released into the medium Formas plaques on a lawn of bacteria which is due to the slower growth of bacteria wher infected by the phage, not cell lysis ...
transformation
... is a bacterium. Once the gene is inserted into the bacterium, the bacterium is grown in a culture medium to produce many copies of the bacterium. The gene is activated and the bacterium begins to produce the protein that the gene codes for. One real-life application of this is the production of huma ...
... is a bacterium. Once the gene is inserted into the bacterium, the bacterium is grown in a culture medium to produce many copies of the bacterium. The gene is activated and the bacterium begins to produce the protein that the gene codes for. One real-life application of this is the production of huma ...
Chapter 20
... APPLICATION Genes conferring useful traits, such as pest resistance, herbicide resistance, delayed ripening, and increased nutritional value, can be transferred from one plant variety or species to another using the Ti plasmid as a vector. ...
... APPLICATION Genes conferring useful traits, such as pest resistance, herbicide resistance, delayed ripening, and increased nutritional value, can be transferred from one plant variety or species to another using the Ti plasmid as a vector. ...
Transformations, Cloning
... Transforming Bacteria Transforming is the process of introducing foreign DNA (plasmid) into host cells Competent bacterial cells are those that are able to take in foreign DNA E. coli is not naturally competent, and is made competent using calcium chloride and heat shock treatment ...
... Transforming Bacteria Transforming is the process of introducing foreign DNA (plasmid) into host cells Competent bacterial cells are those that are able to take in foreign DNA E. coli is not naturally competent, and is made competent using calcium chloride and heat shock treatment ...
Microbial Metabolism
... • Disinfection is the process of limiting or inhibiting microbial growth on a surface • Antisepsis is the process of reducing or limiting microorganisms on a living tissue • Sepsis is bacterial contamination • -cide = to kill • -stat = to inhibit ...
... • Disinfection is the process of limiting or inhibiting microbial growth on a surface • Antisepsis is the process of reducing or limiting microorganisms on a living tissue • Sepsis is bacterial contamination • -cide = to kill • -stat = to inhibit ...
Ch6 XC Test - cloudfront.net
... •Students understand that all the information required by organisms to maintain life is encoded in the arrangement of nucleotides in their DNA. •Students understand that the coding and decoding of DNA is the same among all organisms, which makes possible the expression of a human gene by bacteria. • ...
... •Students understand that all the information required by organisms to maintain life is encoded in the arrangement of nucleotides in their DNA. •Students understand that the coding and decoding of DNA is the same among all organisms, which makes possible the expression of a human gene by bacteria. • ...
+ – DNA
... • Why is each person’s DNA pattern different? – sections of “junk” DNA • doesn’t code for proteins • made up of repeated patterns ...
... • Why is each person’s DNA pattern different? – sections of “junk” DNA • doesn’t code for proteins • made up of repeated patterns ...
Lambda Vectors and their replication
... • Double stranded DNA molecule • 5' twelve-base-pair sticky ends (cos sites) • It is used as a cloning vector, accommodating fragments of DNA up to 15 kilobase pairs long. For larger pieces, the cosmid or YAC’s are used. • Will accept foreign DNA and still complete their life cycle. • Distinguish ce ...
... • Double stranded DNA molecule • 5' twelve-base-pair sticky ends (cos sites) • It is used as a cloning vector, accommodating fragments of DNA up to 15 kilobase pairs long. For larger pieces, the cosmid or YAC’s are used. • Will accept foreign DNA and still complete their life cycle. • Distinguish ce ...
Scientific Writing
... adenovirus, to proliferate. They are capable of infecting both dividing & non dividing cells, & in the absence of a helper virus integrate into a specific point of the host genome (19q 13-qter) at a high frequency. When used as a vector, the rep & cap genes are replaced by the transgene & its associ ...
... adenovirus, to proliferate. They are capable of infecting both dividing & non dividing cells, & in the absence of a helper virus integrate into a specific point of the host genome (19q 13-qter) at a high frequency. When used as a vector, the rep & cap genes are replaced by the transgene & its associ ...
Bacterial Genetics 2
... genome, lambda can only incorporate into a specific site, called attλ. The gal gene is on one side of attλ and the bio gene (biotin synthesis) is on the other side. • Sometimes when lambda come out of the chromosome at the end of the lysogenic phase, it crosses over at the wrong point. This is very ...
... genome, lambda can only incorporate into a specific site, called attλ. The gal gene is on one side of attλ and the bio gene (biotin synthesis) is on the other side. • Sometimes when lambda come out of the chromosome at the end of the lysogenic phase, it crosses over at the wrong point. This is very ...
Cloning of genes from genomic DNA Parts 4 and 5: Ligation and
... the cut PCR product and the cut vector together, thereby cloning our gene. The “sticky” ends (5’ overhangs) created from the restriction enzyme digestions will allow the XbaI end of the plasmid to basepair with the XbaI end of the PCR product. The HinDIII ends will also basepair to each other. Then ...
... the cut PCR product and the cut vector together, thereby cloning our gene. The “sticky” ends (5’ overhangs) created from the restriction enzyme digestions will allow the XbaI end of the plasmid to basepair with the XbaI end of the PCR product. The HinDIII ends will also basepair to each other. Then ...
news and views feature
... transfers are ordinarily discovered during the construction of a phylogenetic tree of an individual protein. When two sequences from otherwise distantly related organisms are found to be more similar to each other than pairs of sequences from known closer relatives, horizontal gene transfer is suspe ...
... transfers are ordinarily discovered during the construction of a phylogenetic tree of an individual protein. When two sequences from otherwise distantly related organisms are found to be more similar to each other than pairs of sequences from known closer relatives, horizontal gene transfer is suspe ...
Slide 1
... plasmid) which contain genetic information necessary for their own replication 2. Single circular double stranded DNA molecule ...
... plasmid) which contain genetic information necessary for their own replication 2. Single circular double stranded DNA molecule ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.