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KNOW THESE GOALS FOR UNDERSTANDING BEFORE COMING!
•Students understand that all the information required by organisms to
maintain life is encoded in the arrangement of nucleotides in their DNA.
•Students understand that the coding and decoding of DNA is the same
among all organisms, which makes possible the expression of a human gene by bacteria.
•Students understand how the processes of transcription and translation
facilitate the transfer of information from DNA to proteins.
•Students recognize that the traits of organisms are determined by the
expression of specific genes in their DNA.
•Students understand how gene expression is regulated.
•Students understand how protein functions are responsible for the traits of an organism.
•Students recognize that a change in the DNA sequence can alter the function
of a protein and can change the traits of an organism.
•Students know how bacteria can be genetically modified to make new products.
•Students understand the reciprocal relationship between basic science research and
technology development: They understand that the discoveries of plasmids, restriction
enzymes, and ligases during basic research have generated the tools and techniques
of biotechnology; in turn, the tools and techniques of biotechnology are enabling scientists
to reach deeper understandings aboutgenes and the function of their products in a cell.
•Students understand the purpose of using controls in scientific investigations
1. Go onto http://www.amgenbiotechexperience.com/curriculum
2. Download Student Guide to Abridged Genetic Engineering Sequence
3. Open Student Guide
4. Read story of diabetes and know the correlation of diabetes to genetic engineering today!
5. BEFORE coming to school on Jan 7, PRINT OUT these questions:
Ch1 Questions, p. 32
Ch 2a Questions, p. 51
Ch 4a Questions, p. 67
Ch 5a Questions, p.84
Ch 6 Questions, p.101
6. PRINT OUT or COPY & Complete ALL the BEFORE THE LAB Questions prior to the
lab that is scheduled for that day(see the expected lab schedule below).
AMGEN SCHEDULE ’13-14
TUESDAY, JAN 7
Set up 37o C water bath for plasmid incubation
1. Lab 1—Pipetting Ex2--Intro to Microvolumetrics and pipetting, using unknowns & running gel
2. Lab 2a—Para-R Restriction Digest using BamH I and Hind III to cut plasmids, incubate at
least 60min at 37o C
3. Paper Plasmid Cloning Lab
NUTRITION
4. *DNA Extraction Lab—Strawberry, Kiwi or Wheat Germ or
Ch6 XC Test
5. Lab 4a—Confirmation of pARA-R Restriction Digest using Gel Electrophoresis
LUNCH
6. *LAB 4—PAPER CHROMATOGRAPHY & PHOTOSYNTHESIS
Setup Culture of Red Colonies into LB tube for Purification
WEDNESDAY, JAN 8
1. *Lab 6— CELL RESPIRATION
NUTRITION
2. Transforming E.coli with a Recombinant Plasmid using competent cells @ -80 C. NEEDS TO
INCUBATE @ 37o C at least overnite for colony growth
3. *LAB 5—Factors Affecting Photosynthetic Rate
o
LUNCH
THURSDAY, JAN 9
1. Lab 6—Preparing an Overnight Culture of E. coli--demo
2. Lab 8—Amplification of the tPA Locus using the Polymerase Chain Reaction
FRIDAY, JAN 10
1. Lab 7—Purification of GFP from an Overnight Culture using column chromatography
2. Lab 8 (cont)—running of gel from PCR results
*NOT a part of the AMGEN BioTech Experience Sequence
OTHER LABS TO BE INCORPORATED INTO THIS 4-DAY TIMEFRAME:
PAPER PLASMID LAB
LAB 6—CELL RESPIRATION
LAB 5—PAPER CHROMATOGRAPHY & PHOTOSYNTHESIS
LAB 9—TRANSPIRATION
DNA EXTRACTION—USING STRAWBERRIES, KIWIS &/OR WHEAT GERM
PROTEIN PURIFICATION