Download Name Bozeman Transformation Lab http://www.youtube.com/watch

Name ___________________________
Bozeman Transformation Lab
http://www.youtube.com/watch?v=OZyFX9megs8&feature=youtu.be
Watch the video by Paul Anderson describing our lab in class on cloning a gene
using the transformation technique. Answer the questions below.
1. Define transformation.
2. Which plasmid (circular DNA) are we using in our lab? Where does it come
from?
3. What does the technique gel electrophoresis allow us to do?
4. Why are plasmids present in bacteria?
5. Describe the different regions on the plasmid we are using? Specifiy the sites
labeled “ori”, “amp”, “GFP”, and “araC”.
6. Describe the four different plates used in this lab. Define what is present in
each plate and what we are placing on each plate.
7. What is the function of the transforming liquid CaCl2 in this lab?
8. Predict what will happen to each of the conditions of plating shown below by
drawing a picture in each circle as well as describing the results in words.
a. E. coli (non-transformed) grown on food media called Luria Broth
b. E. coli (non-transformed) grown on food media called Luria Broth where
the antibiotic ampicillin has been added.
c.
E. coli (transformed with plasmid) grown on food media called Luria
Broth
d. E. coli (transformed with plasmid) grown on food media called Luria
Broth where the antibiotic ampicillin has been added.
9. In gel electrophoresis, which end of the electric field does the DNA migrate
to?
10. How are the DNA samples sorted in the gel? Which pieces migrate farthest?
11. What do restriction enzymes do? How do they work?
12. If you cut DNA with different restriction enzymes, what would be the result
after you ran the gel?
13. What is a DNA ladder used for?