Download DNA.ELECTROPHORESISREVIEW

DNA/ELECTROPHORESIS REVIEW1. Why is E.coli used in so many biotech labs?
2. Draw a simple graph showing the growth curve of E.Coli. Label it!
3. Why are there different types of media?
4. What is the difference between broth and agar?
5. Define electrophoresis.
6. Explain how fragments move on a gel.
7. What side of the gel box is DNA placed on? Why?
8. Explain why DNA is called a double helix.
9. What makes up the backbone of DNA? The rungs?
10.
Draw and label the parts of a G nucleotide.
11.
Explain what complementarity is. Give an example for DNA and RNA.
12.
Identify the central dogma of Biology.
13.
Transcribe and translate DNA- write out an example.
14.
Identify the product of transcription and where it occurs.
15.
Describe the process of translation and where it occurs.
16.
Define codon and anticodon. Which one determines the amino acid?
17.
List differences between DNA and RNA.
18.
Explain what an RFLP is how it is used to diagnose sickle cell anemia.
19.
Draw a simple sickle cell gel and explain what the bands mean.
20.
What else can RFLP’s be used for.
21.
Describe how the Rice Krispies simulation is an analogy to protein synthesis.
22. Explain what Alu is and where it is located
23. Draw a simple gel of Alu results and identify genotypes based on a gel.
24. Calculate the following allele frequencies
Genotype
# of students
+/+
8
+/-
3
-/-
22
+
Total alleles
25. What are the positive and negative allele frequencies in the above table?
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DNA/ELECTROPHORESIS REVIEW- DO NOT PASTE THIS INTO NOTEBOOK!
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Why is E.coli used in so many biotech labs?
Draw a simple graph showing the growth curve of E.Coli. Label it!
Why are there different types of media?
What is the difference between broth and agar?
Define electrophoresis.
Explain how fragments move on a gel.
What side of the gel box is DNA placed on? Why?
Explain why DNA is called a double helix.
What makes up the backbone of DNA? The rungs?
Draw and label the parts of a G nucleotide.
Explain what complementarity is. Give an example for DNA and RNA.
Identify the central dogma of Biology.
Transcribe and translate DNA- write out an example.
Identify the product of transcription and where it occurs.
Describe the process of translation and where it occurs.
Define codon and anticodon. Which one determines the amino acid?
List differences between DNA and RNA.
Explain what an RFLP is how it is used to diagnose sickle cell anemia.
Describe how the Rice Krispies simulation is an analogy to protein synthesis.
Draw a primary, secondary (alpha and beta) structure. Explain why proteins have different
structures.
21. What is a peptide bond? Draw one.