LABORATORY 2: LIGATION OF DNA FRAGMENTS
... without insert). This could be prevented by dephosphorylation of the linearized vector but doesn't always work completely. Ligation of DNA in a mixture routinely results in a variety of potential products. 1. Only circularized plasmids will survive in the bacteria. This eliminates all ligation produ ...
... without insert). This could be prevented by dephosphorylation of the linearized vector but doesn't always work completely. Ligation of DNA in a mixture routinely results in a variety of potential products. 1. Only circularized plasmids will survive in the bacteria. This eliminates all ligation produ ...
Chapter 13 Vocabulary Name
... Student answers will vary but may include comparing genomes or studying cellular development. 3. List three ways genetic recombination occurs in bacteria. By joining together and passing genes, by picking up genes carried by viruses, and by taking up pieces of DNA from the surrounding environment. C ...
... Student answers will vary but may include comparing genomes or studying cellular development. 3. List three ways genetic recombination occurs in bacteria. By joining together and passing genes, by picking up genes carried by viruses, and by taking up pieces of DNA from the surrounding environment. C ...
Kretsu Anna PFUR
... Porcine insulin differs from human insulin by replacement of a Cterminus of the B-chain Ala30Thr. Replacement of threonine by alanine is carried out by enzyme-catalyzed cleavage of alanine and protected connection instead of the carboxyl group of threonine residue present in the reaction mixture in ...
... Porcine insulin differs from human insulin by replacement of a Cterminus of the B-chain Ala30Thr. Replacement of threonine by alanine is carried out by enzyme-catalyzed cleavage of alanine and protected connection instead of the carboxyl group of threonine residue present in the reaction mixture in ...
BioSc 231 Exam 5 2003
... _____Genetic engineering of eukaryotic cells is accomplished using A. Direct injection of DNA into fertilized eggs B. Retroviral vectors C. Embryonic stem cells D. Gene targeting E. All of the above _____ Differing sizes of restriction fragments produced from the alleles of a gene constitute A. a s ...
... _____Genetic engineering of eukaryotic cells is accomplished using A. Direct injection of DNA into fertilized eggs B. Retroviral vectors C. Embryonic stem cells D. Gene targeting E. All of the above _____ Differing sizes of restriction fragments produced from the alleles of a gene constitute A. a s ...
Recombinant DNA Technology
... origin sequences needed for replication and preservation in yeast cells. ...
... origin sequences needed for replication and preservation in yeast cells. ...
PCR (BASIC REQUIREMENT, copied from last semester lecture
... are used in recombinant DNA research to transfer genes between cells. There are many types of plasmids, for example: (1) Fertility (F) plasmids which contain tra (transfer)-genes. They are capable of conjugation (transfer of genetic material between bacteria which are touching). (2) Resistance-(R) p ...
... are used in recombinant DNA research to transfer genes between cells. There are many types of plasmids, for example: (1) Fertility (F) plasmids which contain tra (transfer)-genes. They are capable of conjugation (transfer of genetic material between bacteria which are touching). (2) Resistance-(R) p ...
Generation of Highly Site-Specific DNA Double
... DNA double strand breaks (DSBs) are a common and important form of DNA damage that can be generated by exogenous agents such as ionizing radiation, and by endogenous agents such as reactive oxygen species. DSBs are also generated during nucleic acid metabolism. These “physiologic” DSBs play roles in ...
... DNA double strand breaks (DSBs) are a common and important form of DNA damage that can be generated by exogenous agents such as ionizing radiation, and by endogenous agents such as reactive oxygen species. DSBs are also generated during nucleic acid metabolism. These “physiologic” DSBs play roles in ...
Suppressors
... The motivation for most suppressors screens is to find more genes that affect the same biological process as the original gene. Two types of suppression: I. Suppression – a mutation in one gene alleviates the defect of a mutation in another gene—“low-copy” suppressor. II. Suppression – overexpressio ...
... The motivation for most suppressors screens is to find more genes that affect the same biological process as the original gene. Two types of suppression: I. Suppression – a mutation in one gene alleviates the defect of a mutation in another gene—“low-copy” suppressor. II. Suppression – overexpressio ...
Genetics Review Questions March 2013
... 12. Identify the structures of adenine, guanine, thymine, and cytosine. Which nucleotides pair-up? 13. What is the shape of a DNA molecule like? Describe it. Mendel and Genetics 15. Who was the first to demonstrate the basis of heredity? What are his 2 laws? When is the 2nd law not true? 16. What is ...
... 12. Identify the structures of adenine, guanine, thymine, and cytosine. Which nucleotides pair-up? 13. What is the shape of a DNA molecule like? Describe it. Mendel and Genetics 15. Who was the first to demonstrate the basis of heredity? What are his 2 laws? When is the 2nd law not true? 16. What is ...
Survey of the Phyla-ProkaryoteON
... males have a plasmid (sex factor) which is replicated. When a F+ and F- meet, they form a cytoplasmic bridge or pilus. The copied F+ plasmid is transferred to F- making it F+. ...
... males have a plasmid (sex factor) which is replicated. When a F+ and F- meet, they form a cytoplasmic bridge or pilus. The copied F+ plasmid is transferred to F- making it F+. ...
Genetics and Recombinant DNA
... Extrachromosomal, circular small (2-3 kb) DNA in a bacterial cell which can replicate independently but which cannot integrate into the host chromosome. Drug resistance plasmids are not essential for the cell's growth, but confer antibiotic resistance. Plasmids used for molecular cloning ha ...
... Extrachromosomal, circular small (2-3 kb) DNA in a bacterial cell which can replicate independently but which cannot integrate into the host chromosome. Drug resistance plasmids are not essential for the cell's growth, but confer antibiotic resistance. Plasmids used for molecular cloning ha ...
Genetic Mutations & Genetic Engineering
... • EX: Inversions, duplications, deletions & translocations • Some neither help nor harm, some are beneficial for genetic variation, some cause disorders and cancer ...
... • EX: Inversions, duplications, deletions & translocations • Some neither help nor harm, some are beneficial for genetic variation, some cause disorders and cancer ...
tools of genetic engineering
... A selectable marker is usually a gene that confers resistance to an antibiotic that would otherwise kill the cells. Recombinant DNA is introduced into the organism from which the replication sequences were obtained, then the foreign DNA will be replicated along with the host cell's DNA in the transg ...
... A selectable marker is usually a gene that confers resistance to an antibiotic that would otherwise kill the cells. Recombinant DNA is introduced into the organism from which the replication sequences were obtained, then the foreign DNA will be replicated along with the host cell's DNA in the transg ...
BIOTECHNOLOGY
... Generation of DNA fragments using restriction endonucleases Construction of a recombinant DNA molecule Introduction into a host cell Selection Cells that have been successfully transformed must be isolated (usually by antibiotic resistance) The vectors used for cloning usually carry an antibioticres ...
... Generation of DNA fragments using restriction endonucleases Construction of a recombinant DNA molecule Introduction into a host cell Selection Cells that have been successfully transformed must be isolated (usually by antibiotic resistance) The vectors used for cloning usually carry an antibioticres ...
Chapter 16 Review
... complementary to each other, they can be joined together, A. even though the source of the DNA is different B. even though the source of the DNA is the same C. but the “sticky ends” will most likely have to be modified ...
... complementary to each other, they can be joined together, A. even though the source of the DNA is different B. even though the source of the DNA is the same C. but the “sticky ends” will most likely have to be modified ...
DNA TECHNOLOGY
... Forensic uses of DNA such as DNA fingerprinting Agricultural uses such as manipulatingplant genes and making transgenic plants. ...
... Forensic uses of DNA such as DNA fingerprinting Agricultural uses such as manipulatingplant genes and making transgenic plants. ...
... Neurospora transformants were observed with both types of plasmid DNA minipreps (Table I). The alkaline extraction preparations proved to be the most effective for transformation. However, in all cases the number of transformants obtained was substantially lower (one-fifth to one-twentieth) than the ...
Evolution of bacterial resistance to antibiotics during the last three
... the production of a phosphotransferase APH(3’)-I. Transfer of resistance was achieved by electroporation of a competent strain (E. coli DH5α) with the total DNA of H. influenzae, followed by selection with ampicillin and kanamycin. Using electroporation, 180 colonies of E. coli resistant to ampicill ...
... the production of a phosphotransferase APH(3’)-I. Transfer of resistance was achieved by electroporation of a competent strain (E. coli DH5α) with the total DNA of H. influenzae, followed by selection with ampicillin and kanamycin. Using electroporation, 180 colonies of E. coli resistant to ampicill ...
Chapter 20: Biotechnology - Biology E
... 8a. What is the purpose of a genomic library? The complete set of plasmid-containing cell clones, each carrying copies of a particular segment from the initial genome, is referred to as a genomic library. Each “plasmid clone” in the library is like a book containing specific information. Historical ...
... 8a. What is the purpose of a genomic library? The complete set of plasmid-containing cell clones, each carrying copies of a particular segment from the initial genome, is referred to as a genomic library. Each “plasmid clone” in the library is like a book containing specific information. Historical ...
et al.
... are maintained in yeast as circular DNA molecules even the YCp vectors, which possess yeast centromeres. Thus, none of these vectors resembles the normal yeast chromosomes which have a linear structure. The ends of all yeast chromosomes, like those of all other linear eukaryotic chromosomes, have un ...
... are maintained in yeast as circular DNA molecules even the YCp vectors, which possess yeast centromeres. Thus, none of these vectors resembles the normal yeast chromosomes which have a linear structure. The ends of all yeast chromosomes, like those of all other linear eukaryotic chromosomes, have un ...
15.2 Study Workbook
... Recombinant-DNA technology can change the genetic composition of living organisms. Plasmids are circular DNA molecules found in bacteria and yeasts; they are widely used by scientists studying recombinant DNA, because DNA joined to a plasmid can be replicated. A genetic marker is a gene that is used ...
... Recombinant-DNA technology can change the genetic composition of living organisms. Plasmids are circular DNA molecules found in bacteria and yeasts; they are widely used by scientists studying recombinant DNA, because DNA joined to a plasmid can be replicated. A genetic marker is a gene that is used ...
013368718X_CH15_229-246.indd
... Recombinant-DNA technology can change the genetic composition of living organisms. Plasmids are circular DNA molecules found in bacteria and yeasts; they are widely used by scientists studying recombinant DNA, because DNA joined to a plasmid can be replicated. A genetic marker is a gene that is used ...
... Recombinant-DNA technology can change the genetic composition of living organisms. Plasmids are circular DNA molecules found in bacteria and yeasts; they are widely used by scientists studying recombinant DNA, because DNA joined to a plasmid can be replicated. A genetic marker is a gene that is used ...
Quiz 3 Solutions
... Are the genes for size and color linked or unlinked? Use the data above to justify your answer. The genes for size and color are linked. This is apparent from the 1:1 ratio of the F1 generation and the lack of the double recessive (Small White ants) phenotype and the double dominant (Large Red). Nei ...
... Are the genes for size and color linked or unlinked? Use the data above to justify your answer. The genes for size and color are linked. This is apparent from the 1:1 ratio of the F1 generation and the lack of the double recessive (Small White ants) phenotype and the double dominant (Large Red). Nei ...
06MicrobialGenetExamII
... 4.) The single copy F plasmid replicates only once per cell generation. Thus at the time of cell division, there are only two copies of the plasmid per cell. Assuming that the plasmid is sorted randomly, calculate (or draw) how often the plasmid should be lost by chance each generation. (5pts) ...
... 4.) The single copy F plasmid replicates only once per cell generation. Thus at the time of cell division, there are only two copies of the plasmid per cell. Assuming that the plasmid is sorted randomly, calculate (or draw) how often the plasmid should be lost by chance each generation. (5pts) ...
pp Multiple Choice Identify the letter of the choice that best
... c. is possible only between closely related species d. does not cut DNA e. does not involve enzymes 3. Small circular molecules of "extra" DNA in bacteria are called ____. a. plasmids b. desmids c. pilus d. F-particles e. transferins 4. Which is not true of plasmids? a. They are self-reproducing cir ...
... c. is possible only between closely related species d. does not cut DNA e. does not involve enzymes 3. Small circular molecules of "extra" DNA in bacteria are called ____. a. plasmids b. desmids c. pilus d. F-particles e. transferins 4. Which is not true of plasmids? a. They are self-reproducing cir ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.