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... the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the same restriction enzyme used to cut out the human gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by g ...
... the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the same restriction enzyme used to cut out the human gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by g ...
32 Fungal Genetics Newsletter Ursula Kües , Michaela J. Klaus
... Multiple cotransformation of an A-null strain. In another set of experiments, using the tryptophan auxotrophic C. cinereus strain NA2 ( A B6 ade-8 trp-1.1,1.6) and plasmid pCc1001 for selection, we studied uptake of up to four different plasmids in transformation. Strain NA2 is an A-null mutant whos ...
... Multiple cotransformation of an A-null strain. In another set of experiments, using the tryptophan auxotrophic C. cinereus strain NA2 ( A B6 ade-8 trp-1.1,1.6) and plasmid pCc1001 for selection, we studied uptake of up to four different plasmids in transformation. Strain NA2 is an A-null mutant whos ...
Chapter 8 Bacterial Genetics
... • Direct selection: cells inoculated onto medium that supports growth of mutant but not parent • for example, antibiotic-resistant mutants exposed to ...
... • Direct selection: cells inoculated onto medium that supports growth of mutant but not parent • for example, antibiotic-resistant mutants exposed to ...
Part 1: Genetic Engineering
... 2. Explain the significance of “sticky ends” and why they were given that name. Vectors: 3. Diagram a typical designed plasmid vector. Label and define each of the following parts: a. The ori b. The multiple cloning sequence (you might need the internet) c. Selectable markers--give two examples of g ...
... 2. Explain the significance of “sticky ends” and why they were given that name. Vectors: 3. Diagram a typical designed plasmid vector. Label and define each of the following parts: a. The ori b. The multiple cloning sequence (you might need the internet) c. Selectable markers--give two examples of g ...
Unit 4: Viruses Intro Video Anatomy of a Virus
... • Capsids built from large numbers of protein subunits called CAPSOMERES • Most complex capsids found in viruses that infect bacteria – BACTERIOPHAGES (T1T7). They have protein tail piece with tail fibers that attach to the bacterium ...
... • Capsids built from large numbers of protein subunits called CAPSOMERES • Most complex capsids found in viruses that infect bacteria – BACTERIOPHAGES (T1T7). They have protein tail piece with tail fibers that attach to the bacterium ...
Physical map of the aromatic amine and m-toluate
... present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in leng ...
... present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in leng ...
Construction of recombinant plasmid carrying the λ DNA fragment
... Other confirmation for recombinant plasmid insertion into chromosome of bacteria was obtained from specialized transduction experiments. A transducing phages were prepared from appropriate lysogens by heat induction. Lft lysates were used to infect E.coli C600 and Ap r C 6 1 i m m transductants were ...
... Other confirmation for recombinant plasmid insertion into chromosome of bacteria was obtained from specialized transduction experiments. A transducing phages were prepared from appropriate lysogens by heat induction. Lft lysates were used to infect E.coli C600 and Ap r C 6 1 i m m transductants were ...
Presented By: Chantille Haynes, Hilary Price, and Richard Dalton
... -Mutants are isolated by washing the filter. -The washed cells must be plated on a range of different media, each supplemented with one specific chemical. -This will aid in determining the specific requirements of any given auxotroph. What results might we expect? ...
... -Mutants are isolated by washing the filter. -The washed cells must be plated on a range of different media, each supplemented with one specific chemical. -This will aid in determining the specific requirements of any given auxotroph. What results might we expect? ...
Chapter 9 – DNA-Based Information Technologies
... overhang of ssDNA on each strand blunt ends – cut directly across both strands leaving all dsDNA ...
... overhang of ssDNA on each strand blunt ends – cut directly across both strands leaving all dsDNA ...
This is a test - DNALC::Protocols
... A vector is a DNA molecule that is used as a vehicle to carry foreign DNA. We are using the simplest bacterial vectors, plasmids, which are small rings of DNA that exist independently of the main bacterial chromosome. They can be replicated and passed on to offspring. Plasmids can be used as tools t ...
... A vector is a DNA molecule that is used as a vehicle to carry foreign DNA. We are using the simplest bacterial vectors, plasmids, which are small rings of DNA that exist independently of the main bacterial chromosome. They can be replicated and passed on to offspring. Plasmids can be used as tools t ...
Cloning Genes
... Note: practical limit on the size of DNA cloned into a vectors (plasmid: 5-10 kbp, phage: 45 kbp) ...
... Note: practical limit on the size of DNA cloned into a vectors (plasmid: 5-10 kbp, phage: 45 kbp) ...
Spr01Exam II Answer Key
... 18.) Which mutations belong to the same complement group(s). At least how many genes or complementation groups do you suspect are involved in the red (red, mucoid) pathway? (10pts) If the two mutations can restore the red mucoid phenotype, then these mutations are likely to be in separate genes. Mut ...
... 18.) Which mutations belong to the same complement group(s). At least how many genes or complementation groups do you suspect are involved in the red (red, mucoid) pathway? (10pts) If the two mutations can restore the red mucoid phenotype, then these mutations are likely to be in separate genes. Mut ...
Lateral gene transfer in prokaryotic genomes: which genes
... protects from related phages and sometimes confers advantages (toxin genes in phages of C. diphteriae). The phage can later be induced to exit the chromosome and replicate (lytic cycle). Rarely the phage packages neighboring host genes, leaving some of its DNA behind. Thus, a phage can shuttle DNA b ...
... protects from related phages and sometimes confers advantages (toxin genes in phages of C. diphteriae). The phage can later be induced to exit the chromosome and replicate (lytic cycle). Rarely the phage packages neighboring host genes, leaving some of its DNA behind. Thus, a phage can shuttle DNA b ...
Name Period ______ Ms Foglia • AP Biology Date LAB: CLONING
... and A whenever it encounters the six-base sequence AAGCTT. 4. Examine the DNA sequence for the plasmid and the jellyfish gene. Which restriction enzyme should you use to cut the plasmid? The jellyfish gene? Remember, when you cut each gene, you need to retain the start and stop sequences. Should you ...
... and A whenever it encounters the six-base sequence AAGCTT. 4. Examine the DNA sequence for the plasmid and the jellyfish gene. Which restriction enzyme should you use to cut the plasmid? The jellyfish gene? Remember, when you cut each gene, you need to retain the start and stop sequences. Should you ...
pGLO
... protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, you will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small circular pie ...
... protein which causes them to glow a brilliant green color under ultraviolet light. In this activity, you will learn about the process of moving genes from one organism to another with the aid of a plasmid. In addition to one large chromosome, bacteria naturally contain one or more small circular pie ...
Prokaryotes - Nicholls State University
... cells. One set of genes encodes proteins that form a pilus, or tube, between F+ cells and F- cells. The completed tube is called a conjugation bridge. ...
... cells. One set of genes encodes proteins that form a pilus, or tube, between F+ cells and F- cells. The completed tube is called a conjugation bridge. ...
Biotechnology - University of California, Los Angeles
... 1. Circular piece of DNA that replicates independently of the chromosome(s) 2. Protein that can “cut” DNA at precise spots 3. Purposeful manipulation of DNA 4. Overhanging sequence of bases left after DNA is “cut” 5. Substance that can be used to identify cells with our gene of interest ...
... 1. Circular piece of DNA that replicates independently of the chromosome(s) 2. Protein that can “cut” DNA at precise spots 3. Purposeful manipulation of DNA 4. Overhanging sequence of bases left after DNA is “cut” 5. Substance that can be used to identify cells with our gene of interest ...
Curriculum Walk Through
... Basic content, lab techniques, and skills used by researchers in the biotechnology field Choice of lab sequences All specialized equipment provided including consumables at no cost Teachers provide limited, commonplace equipment (beakers, etc) ...
... Basic content, lab techniques, and skills used by researchers in the biotechnology field Choice of lab sequences All specialized equipment provided including consumables at no cost Teachers provide limited, commonplace equipment (beakers, etc) ...
prokaryotic cell
... ral gene transfer, is a process in which an org anism transfers genetic material to anoth er cell that is not its offspring. The ability of Bacteria and Archaea to adapt to new environm ents as a part of bacterial evolution, most freque ntly results from the acquisition of new genes thr ough horizon ...
... ral gene transfer, is a process in which an org anism transfers genetic material to anoth er cell that is not its offspring. The ability of Bacteria and Archaea to adapt to new environm ents as a part of bacterial evolution, most freque ntly results from the acquisition of new genes thr ough horizon ...
L27- Cloning
... How do we know which of all these colonies came from a cell that took up a plasmid carrying RPE65? ...
... How do we know which of all these colonies came from a cell that took up a plasmid carrying RPE65? ...
Purification and Characterization of a DNA Plasmid Part A
... Midiprep resin. Mix by swirling. This allows the DNA to bind to the resin in batch mode. Discard the pellet. 5. Place the column tip (labeled with your initials) into the vacuum manifold. Pour the DNAresin slurry into the column. Apply vacuum to pack the slurry into the column. Once the "flow-throug ...
... Midiprep resin. Mix by swirling. This allows the DNA to bind to the resin in batch mode. Discard the pellet. 5. Place the column tip (labeled with your initials) into the vacuum manifold. Pour the DNAresin slurry into the column. Apply vacuum to pack the slurry into the column. Once the "flow-throug ...
Molecular Cloning
... vector or plasmid. The plasmid carrying genes for antibiotic resistance, and a DNA strand, which contains the gene of interest, are both cut with the same restriction endonuclease. The plasmid is opened up and the gene is freed from its parent DNA strand. They have complementary "sticky ends." The o ...
... vector or plasmid. The plasmid carrying genes for antibiotic resistance, and a DNA strand, which contains the gene of interest, are both cut with the same restriction endonuclease. The plasmid is opened up and the gene is freed from its parent DNA strand. They have complementary "sticky ends." The o ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.