Mechanistic insights into the regulation of metabolic enzymes by
... In addition to regulating proteasome-dependent degradation, acetylation can also regulate the degradation of metabolic enzymes by lysosomes. Once thought to function primarily in the wholesale breaking down of foreign material such as viruses, bacteria, and worn-out cellular organelles (micro- and m ...
... In addition to regulating proteasome-dependent degradation, acetylation can also regulate the degradation of metabolic enzymes by lysosomes. Once thought to function primarily in the wholesale breaking down of foreign material such as viruses, bacteria, and worn-out cellular organelles (micro- and m ...
Homer1a and Fragile X 1 Disrupted mGluR5
... 10% SDS gel and blotted for Mab1b, actin and αCamKII. The membrane was cut between 75 and 100 kDa. F) Samples were loaded into a 10% SDS gel and blotted for Arc and ERK. ...
... 10% SDS gel and blotted for Mab1b, actin and αCamKII. The membrane was cut between 75 and 100 kDa. F) Samples were loaded into a 10% SDS gel and blotted for Arc and ERK. ...
Articulins and epiplasmins - Journal of Cell Science
... SDS-denatured epiplasm proteins of P. dubius was used (Peck et al., 1991). For the affinity selection of articulin VPV-repeat motif-specific antibodies the central VPV-repeat motif domain of P. dubius articulin p60 was expressed as a fusion protein in E. coli BL 21 cells (see below). 8-9 slots (slot ...
... SDS-denatured epiplasm proteins of P. dubius was used (Peck et al., 1991). For the affinity selection of articulin VPV-repeat motif-specific antibodies the central VPV-repeat motif domain of P. dubius articulin p60 was expressed as a fusion protein in E. coli BL 21 cells (see below). 8-9 slots (slot ...
Life and death of transcriptional co
... Phosphorylation of p300 occurs abundantly during cell proliferation and differentiation.30-33 Cyclin-dependent kinases are directly or indirectly involved in the regulation of p300 and CBP during cell cycle progression.34-36 Site specific serine-threonine phosphorylation have been identified in p300 ...
... Phosphorylation of p300 occurs abundantly during cell proliferation and differentiation.30-33 Cyclin-dependent kinases are directly or indirectly involved in the regulation of p300 and CBP during cell cycle progression.34-36 Site specific serine-threonine phosphorylation have been identified in p300 ...
Role of histone deacetylase 2 and its
... involved in the regulation of the amount, function, and localization of many proteins. For example, phosphorylation is a critical modification for enzymatic activation (5), binding to DNA (6), forming complexes (7), and subcellular localization (8). Polyubiquitination, a continued link of ubiquitin ...
... involved in the regulation of the amount, function, and localization of many proteins. For example, phosphorylation is a critical modification for enzymatic activation (5), binding to DNA (6), forming complexes (7), and subcellular localization (8). Polyubiquitination, a continued link of ubiquitin ...
Hepatocyte Growth Factor Induces Wnt
... Hepatocyte growth factor (HGF) and Wnt signaling pathways have been shown to be important in embryogenesis and carcinogenesis. The aim of this study was to elucidate the mechanism of functional similarities observed in the two pathways. We used normal rat liver, primary hepatocyte cultures and a dom ...
... Hepatocyte growth factor (HGF) and Wnt signaling pathways have been shown to be important in embryogenesis and carcinogenesis. The aim of this study was to elucidate the mechanism of functional similarities observed in the two pathways. We used normal rat liver, primary hepatocyte cultures and a dom ...
Evidence That Ternary Complex (eIF2-GTP-tRNAi
... most of the cytoplasmic TIA-1 and TIAR to accumulate at SGs in these cells (Figure 1, A, B, D–F; top, green). In paired views of the same fields, antibodies specific for small ribosomal subunit proteins S3/3a and S19 (Figure 1, A and B; middle, red) reveal that a significant fraction of the small ri ...
... most of the cytoplasmic TIA-1 and TIAR to accumulate at SGs in these cells (Figure 1, A, B, D–F; top, green). In paired views of the same fields, antibodies specific for small ribosomal subunit proteins S3/3a and S19 (Figure 1, A and B; middle, red) reveal that a significant fraction of the small ri ...
Biology, 7e (Campbell) Chapter 9: Cellular Respiration: Harvesting
... compound pyruvate can be catabolized in the citric acid cycle. First, however, the pyruvate 1) loses a carbon, which is given off as a molecule of CO2, 2) is oxidized to form a two-carbon compound called acetate, and 3) is bonded to coenzyme A. These three steps result in the formation of A) acetyl ...
... compound pyruvate can be catabolized in the citric acid cycle. First, however, the pyruvate 1) loses a carbon, which is given off as a molecule of CO2, 2) is oxidized to form a two-carbon compound called acetate, and 3) is bonded to coenzyme A. These three steps result in the formation of A) acetyl ...
Interactions of liposomes and lipid-based carrier systems with blood
... binding (400–1100 g protein / mol total lipid) was observed. These results are not unexpected since the majority of plasma proteins carry a net negative charge at physiological pH. Anionic lipids have been incorporated into liposomes to increase the trapped volume and improve the loading of various ...
... binding (400–1100 g protein / mol total lipid) was observed. These results are not unexpected since the majority of plasma proteins carry a net negative charge at physiological pH. Anionic lipids have been incorporated into liposomes to increase the trapped volume and improve the loading of various ...
Quantitative determinants of aerobic glycolysis identify flux through
... Effect (the ratio of flux to lactate over that entering the mitochondria) ranged from less than one (primarily oxidative metabolism) to over 95% of glucose being converted to lactate. The dynamic range over which the Warburg Effect was observed in our model allowed us to investigate to other variabl ...
... Effect (the ratio of flux to lactate over that entering the mitochondria) ranged from less than one (primarily oxidative metabolism) to over 95% of glucose being converted to lactate. The dynamic range over which the Warburg Effect was observed in our model allowed us to investigate to other variabl ...
Boundless Study Slides
... • complex A structure consisting of a central atom, molecule, or protein weakly connected to surrounding atoms, molecules, or proteins. • deamination The removal of an amino group from a compound. • disaccharide A sugar, such as sucrose, maltose, or lactose, consisting of two monosaccharides combine ...
... • complex A structure consisting of a central atom, molecule, or protein weakly connected to surrounding atoms, molecules, or proteins. • deamination The removal of an amino group from a compound. • disaccharide A sugar, such as sucrose, maltose, or lactose, consisting of two monosaccharides combine ...
PFK - ePrints USM
... phosphofructokinase (PFK) (ATP-PFK; EC 2.7.1.11) enzyme at 5 oC in the complex medium. The cells of the isolate were ovoid in shape and showed asexual reproduction by budding. L.antarcticum produces intracellular PFK activity in the late exponential growth phase (about 32x108 CFU/mL) after 15 days o ...
... phosphofructokinase (PFK) (ATP-PFK; EC 2.7.1.11) enzyme at 5 oC in the complex medium. The cells of the isolate were ovoid in shape and showed asexual reproduction by budding. L.antarcticum produces intracellular PFK activity in the late exponential growth phase (about 32x108 CFU/mL) after 15 days o ...
Arachidonic Acid Mediates Interleukin-1 and Tumor
... Effect of arachidonic acid on JNK protein kinases. Our recent studies suggested that a signal transduction pathway involving a tyrosine kinase cascade mediated arachidonic acid-induced c-jun transcription.” To further elucidate the components of the signaling route to c-jun transcription by arachido ...
... Effect of arachidonic acid on JNK protein kinases. Our recent studies suggested that a signal transduction pathway involving a tyrosine kinase cascade mediated arachidonic acid-induced c-jun transcription.” To further elucidate the components of the signaling route to c-jun transcription by arachido ...
You can answer the question Yourself with a few
... BCAAs, which include the three amino acids leucine, isoleucine, and valine. These bad boys have been shown to be absolutely key for muscle growth and even for energy during workouts. Whey protein also contains biologically active protein microfractions, such as alpha-lactalbumin, beta-lactoglobulin, ...
... BCAAs, which include the three amino acids leucine, isoleucine, and valine. These bad boys have been shown to be absolutely key for muscle growth and even for energy during workouts. Whey protein also contains biologically active protein microfractions, such as alpha-lactalbumin, beta-lactoglobulin, ...
Secured cutting: controlling separase at the metaphase to anaphase
... DNA is damaged, anaphase onset is delayed to allow repair before sister sequences are segregated from each other. In budding yeast, DNA damage elicits a response pathway which uses two routes that act together to prevent anaphase (Cohen-Fix and Koshland, 1997; Gardner et al., 1999; Sanchez et al., 1 ...
... DNA is damaged, anaphase onset is delayed to allow repair before sister sequences are segregated from each other. In budding yeast, DNA damage elicits a response pathway which uses two routes that act together to prevent anaphase (Cohen-Fix and Koshland, 1997; Gardner et al., 1999; Sanchez et al., 1 ...
A CBS domain-containing pyrophosphatase of Moorella
... Consequently, disruption of PPi metabolism can lead to a variety of pathological conditions [2]. PPi is mainly hydrolysed to Pi (orthophosphate) by PPase (inorganic pyrophosphatase) (EC 3.6.1.1), an enzyme that is essential for life [3–6]. There are two known types of PPase: soluble and integral mem ...
... Consequently, disruption of PPi metabolism can lead to a variety of pathological conditions [2]. PPi is mainly hydrolysed to Pi (orthophosphate) by PPase (inorganic pyrophosphatase) (EC 3.6.1.1), an enzyme that is essential for life [3–6]. There are two known types of PPase: soluble and integral mem ...
Cellular functions of the BRCA tumour
... of BRCA1 at Ser1423 is important for its role in the G2 /M checkpoint, but not for its role in the S-phase checkpoint, as mutation of this site compromised its G2 function [26]. Current evidence suggests that BRCA1 functions as a checkpoint mediator, similar to Mdc1, 53BP1 and claspin, that collecti ...
... of BRCA1 at Ser1423 is important for its role in the G2 /M checkpoint, but not for its role in the S-phase checkpoint, as mutation of this site compromised its G2 function [26]. Current evidence suggests that BRCA1 functions as a checkpoint mediator, similar to Mdc1, 53BP1 and claspin, that collecti ...
Module 1. General principles of metabolism. Metabolism of
... 42. They use diamination reaction for quantitative determination of α-amino acids. What are you use reagent for quantitative determination amino groups in amino acids? A. NaCl. B. KOH. C. NH4NO3. D. * HNO2. E. HCl. 43. Total amount of albumins and globulins in blood plasma for healthy people is: A. ...
... 42. They use diamination reaction for quantitative determination of α-amino acids. What are you use reagent for quantitative determination amino groups in amino acids? A. NaCl. B. KOH. C. NH4NO3. D. * HNO2. E. HCl. 43. Total amount of albumins and globulins in blood plasma for healthy people is: A. ...
Processing Synthesis of Cellulase from Ripening
... Gel Electrophoresis, Protein Blotting, and Immunodetection. Proteins were analyzed by SDS-PAGE using 16 cm 10% polyacrylamide gels and the buffer system of Laemmli (24). For immunodetection of cellulase, proteins were electroblotted to nitrocellulose (Bio-Rad) essentially as described by Burnette (7 ...
... Gel Electrophoresis, Protein Blotting, and Immunodetection. Proteins were analyzed by SDS-PAGE using 16 cm 10% polyacrylamide gels and the buffer system of Laemmli (24). For immunodetection of cellulase, proteins were electroblotted to nitrocellulose (Bio-Rad) essentially as described by Burnette (7 ...
Module 2 General principles of metabolism. Мetabolism of carbohy
... 38. In a ping-pong reaction which does not occur? A. One product is released before a second substrate is bound. B. The enzyme covalently binds a portion of the first substrate. C. * The enzyme is permanently converted to an altered form by the first substrate. D. A group is transferred from one su ...
... 38. In a ping-pong reaction which does not occur? A. One product is released before a second substrate is bound. B. The enzyme covalently binds a portion of the first substrate. C. * The enzyme is permanently converted to an altered form by the first substrate. D. A group is transferred from one su ...
Engineering Cytosolic Acetyl-CoA Metabolism in Saccharomyces
... biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic acetyl-CoA for lipid biosynthesis. Its growth inability on glucose could be restored through directed evolution, which was explained by an in-frame internal deletion in MTH1 (MTH1-∆T). T ...
... biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic acetyl-CoA for lipid biosynthesis. Its growth inability on glucose could be restored through directed evolution, which was explained by an in-frame internal deletion in MTH1 (MTH1-∆T). T ...
2.2.3 Enzymes
... substrate. Some enzymes are involved in the chemical break down of substances into smaller components. Amylase, pepsin or catalase as examples of enzymes involved in chemical breakdown. Other enzymes, such as potato phosphorylase, are involved in synthesis i.e. chemically combining small substances ...
... substrate. Some enzymes are involved in the chemical break down of substances into smaller components. Amylase, pepsin or catalase as examples of enzymes involved in chemical breakdown. Other enzymes, such as potato phosphorylase, are involved in synthesis i.e. chemically combining small substances ...
Development of prokaryotic cell-free systems for synthetic
... Separately, work from the Kim (HJ) lab in 2005 identified strains with the overexpression of molecular chaperones capable of reducing aggregation and improve solubility of eukaryotic proteins such as human erythropoietin (Kang et al., 2005). The work inserted plasmids to overexpress chaperone and he ...
... Separately, work from the Kim (HJ) lab in 2005 identified strains with the overexpression of molecular chaperones capable of reducing aggregation and improve solubility of eukaryotic proteins such as human erythropoietin (Kang et al., 2005). The work inserted plasmids to overexpress chaperone and he ...
GLYCOGENOLYSIS AND GLYCOLYSIS IN MUSCLE
... amounts of starch are very useful to us as foodstuffs. Plants also link glucose molecules together in a complex pattern to form cellulose for structural purposes, but humans lack the enzymes necessary to digest this glucose polymer. There are numerous dietary sources of glucose, including starches, ...
... amounts of starch are very useful to us as foodstuffs. Plants also link glucose molecules together in a complex pattern to form cellulose for structural purposes, but humans lack the enzymes necessary to digest this glucose polymer. There are numerous dietary sources of glucose, including starches, ...
A pervasive role of histone acetyltransferases and deacetylases in
... The NF-kB family of transcription factors is present in most vertebrate cell types as homo- and heterodimers of five structurally related Rel and NF-kB proteins, namely p65 (also called RelA), RelB, c-Rel, NF-kB1 (p50 and its precursor p105) and NF-kB2 (p52 and its precursor p100) [1,2]. The transac ...
... The NF-kB family of transcription factors is present in most vertebrate cell types as homo- and heterodimers of five structurally related Rel and NF-kB proteins, namely p65 (also called RelA), RelB, c-Rel, NF-kB1 (p50 and its precursor p105) and NF-kB2 (p52 and its precursor p100) [1,2]. The transac ...
Phosphorylation
Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Phosphorylation and its counterpart, dephosphorylation, turn many protein enzymes on and off, thereby altering their function and activity. Protein phosphorylation is one type of post-translational modification.Protein phosphorylation in particular plays a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research (as of March 2015, the Medline database returns over 240,000 articles on the subject, largely on protein phosphorylation).