Shaping the metaphase chromosome: coordination of cohesion and
... The cohesin complex purified from HeLa cells can bind to double-stranded DNA and induce the formation of large protein±DNA aggregates in vitro.(10) In the presence of topoisomerase II, cohesin stimulates intermolecular catenation of circular DNA molecules. This activity is in striking contrast to th ...
... The cohesin complex purified from HeLa cells can bind to double-stranded DNA and induce the formation of large protein±DNA aggregates in vitro.(10) In the presence of topoisomerase II, cohesin stimulates intermolecular catenation of circular DNA molecules. This activity is in striking contrast to th ...
Gene Section KSR1 (kinase suppressor of ras 1)
... Raf/MEK/ERK kinase cascade. However, there are several published studies that sustain that KSR1 has catalytic activity. KSR1 is recognized as a pseudokinase, since mammalian KSR1 does not possess the lysine responsible for ATP orientation and hydrolysis in the putative kinase domain. This lysine is ...
... Raf/MEK/ERK kinase cascade. However, there are several published studies that sustain that KSR1 has catalytic activity. KSR1 is recognized as a pseudokinase, since mammalian KSR1 does not possess the lysine responsible for ATP orientation and hydrolysis in the putative kinase domain. This lysine is ...
Defining the impact on yeast ATP synthase of two pathogenic
... necessarily mean that mitochondrial ATP synthesis was not compromised. Indeed as previously shown through the analysis of numerous yeast ATP synthase mutants, the activity of this enzyme needs to be decreased by at least 80% to see an obvious respiratory growth defect, which indicates that ATP synth ...
... necessarily mean that mitochondrial ATP synthesis was not compromised. Indeed as previously shown through the analysis of numerous yeast ATP synthase mutants, the activity of this enzyme needs to be decreased by at least 80% to see an obvious respiratory growth defect, which indicates that ATP synth ...
Microsoft Word (manuscript)
... complex and described it in detail, showing that for association of DPM1 with DPM2 24 Cterminal amino acids of DPM1and Phe 21 and Tyr 23 from DPM2 were important. Furthermore, DPM3 alone was able to stabilize DPM1 and DPM synthase had an enzymatic activity without the DPM2 subunit, however, the act ...
... complex and described it in detail, showing that for association of DPM1 with DPM2 24 Cterminal amino acids of DPM1and Phe 21 and Tyr 23 from DPM2 were important. Furthermore, DPM3 alone was able to stabilize DPM1 and DPM synthase had an enzymatic activity without the DPM2 subunit, however, the act ...
Module 3 Metabolism of carbohydrates, lipids
... D. All of the above E. None of the above 17. ATP is a cosubstrate of the enzyme PFK-1. In most species ATP is also an inhibitor of PFK-1 at higher concentrations. This seems to violate Le Chatelier's Principle. Which statement below would provide a suitable explanation? A. PFK-1 must be phosphorylat ...
... D. All of the above E. None of the above 17. ATP is a cosubstrate of the enzyme PFK-1. In most species ATP is also an inhibitor of PFK-1 at higher concentrations. This seems to violate Le Chatelier's Principle. Which statement below would provide a suitable explanation? A. PFK-1 must be phosphorylat ...
Physical interaction between pRb and cdk9/cyclinT2 complex
... 794 ± 829 in the absence of recognition motifs contained between pRb residues 829 ± 928 (Adams et al., 1999). Our data suggest that this is true also for cdk9 complexes. In fact, cdk9/cyclin T2 binds pRb (835 ± 928), phosphorylates the C-terminal domain (793 ± 928), but fails to phosphorylate the tw ...
... 794 ± 829 in the absence of recognition motifs contained between pRb residues 829 ± 928 (Adams et al., 1999). Our data suggest that this is true also for cdk9 complexes. In fact, cdk9/cyclin T2 binds pRb (835 ± 928), phosphorylates the C-terminal domain (793 ± 928), but fails to phosphorylate the tw ...
... investigated whether differences could be observed in the inclusion bodies formed by the respective mutant AHSV-9 NS2 proteins. Transmission electron microscope analysis of recombinant baculovirus-infected Spodoptera frugiperda insect cells indicated that a mutant NS2 protein in which the serine re ...
AAST_2014_09_Project_10
... Anti Polyphosphate Antibody Inhibits Polyphosphate Driven Coagulation ...
... Anti Polyphosphate Antibody Inhibits Polyphosphate Driven Coagulation ...
Glucosamine-Induced Insulin Resistance in Primary Rat
... of the insulin signaling pathway that includes PI3-Kinase and Akt-1 (protein kinase B). Phosphorylation of GSK-3 reduces its kinase activity, thereby resulting in reduced phosphorylation of glycogen synthase and an increase in its enzymatic activity (Frame, Cohen etal. 2001). In glycolysis, one mole ...
... of the insulin signaling pathway that includes PI3-Kinase and Akt-1 (protein kinase B). Phosphorylation of GSK-3 reduces its kinase activity, thereby resulting in reduced phosphorylation of glycogen synthase and an increase in its enzymatic activity (Frame, Cohen etal. 2001). In glycolysis, one mole ...
DNA REPLICATION CHECKPOINT CONTROL 7. Acknowledgments
... itself. Studies on proteins that are part of the replication apparatus are complicated by the fact that they are usually essential. However, spore germination studies have been ...
... itself. Studies on proteins that are part of the replication apparatus are complicated by the fact that they are usually essential. However, spore germination studies have been ...
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in
... when cells carry mutations in Hsp90Ec they grow more slowly at high temperature [20], exhibit a slight increase in aggregated proteins at high temperature [21], lose adaptive immunity conferred by the CRISPR system [22] and show a subtle defect in motility [23]. Additionally, when Hsp90Ec is overexp ...
... when cells carry mutations in Hsp90Ec they grow more slowly at high temperature [20], exhibit a slight increase in aggregated proteins at high temperature [21], lose adaptive immunity conferred by the CRISPR system [22] and show a subtle defect in motility [23]. Additionally, when Hsp90Ec is overexp ...
Diapositive 1
... Figure S2. Potential regulation of Par-4 phosphorylation by Phosphatase PP1 (A) PC-3 cells were treated with the phosphatase inhibitor CalA (20 nM) and, at indicated time points, phosphorylated Par-4 (upward shift) was assessed by western blotting. (B) Cells were treated for 1h with CalA (20 nM) or ...
... Figure S2. Potential regulation of Par-4 phosphorylation by Phosphatase PP1 (A) PC-3 cells were treated with the phosphatase inhibitor CalA (20 nM) and, at indicated time points, phosphorylated Par-4 (upward shift) was assessed by western blotting. (B) Cells were treated for 1h with CalA (20 nM) or ...
In Vitro Reconstitution of SCF Substrate Ubiquitination with Purified
... lacking eukaryotic posttranslational modifications. While baculovirus expression systems may require slightly more expertise and considerably more time, posttranslational modifications may occur during expression in insect cells, potentially eliminating the requirement for pretreating substrates pri ...
... lacking eukaryotic posttranslational modifications. While baculovirus expression systems may require slightly more expertise and considerably more time, posttranslational modifications may occur during expression in insect cells, potentially eliminating the requirement for pretreating substrates pri ...
Insulin action on skeletal muscle protein metabolism during
... and cofactors involved in the proteolytic pathways correlated with results obtained in goats. The ATP-ubiquitin-proteasome dependent is, no doubt, a target for insulin in vivo. This does not preclude that insulin may have an inhibitory effect on muscle proteolysis by alternative mechanisms [24]. ...
... and cofactors involved in the proteolytic pathways correlated with results obtained in goats. The ATP-ubiquitin-proteasome dependent is, no doubt, a target for insulin in vivo. This does not preclude that insulin may have an inhibitory effect on muscle proteolysis by alternative mechanisms [24]. ...
as PDF
... variation of this technique, difference gel electrophoresis (DIGE), proteins from two samples are first labeled with different fluorescent dyes and then mixed, making it possible to compare two different samples on a single gel. Two fluorescence images are recorded and overlayed, and differentially ...
... variation of this technique, difference gel electrophoresis (DIGE), proteins from two samples are first labeled with different fluorescent dyes and then mixed, making it possible to compare two different samples on a single gel. Two fluorescence images are recorded and overlayed, and differentially ...
Basic Science for Clinicians
... phosphofructokinase-1 (PFK-1). PFK-1 acts as a metabolic gatekeeper, regulating the entry of glucose into glycolysis. However, the molecular signaling role of AMP is dramatically extended by activation of the AMPK pathway. Because many more proteins are regulated by AMPK than contain specialized AMP ...
... phosphofructokinase-1 (PFK-1). PFK-1 acts as a metabolic gatekeeper, regulating the entry of glucose into glycolysis. However, the molecular signaling role of AMP is dramatically extended by activation of the AMPK pathway. Because many more proteins are regulated by AMPK than contain specialized AMP ...
Generation of ATP during Cytochrome-linked
... nicotinic acid, I mg; p-amino benzoic acid, 0-1mg; pH 6.5. For growth experiments with glycerol the synthetic medium described by Bauchop & Elsden (1960) was used. Growthlimiting concentrations of glucose or fructose (up to 5,umol/ml), sodium lactate (up to 30 ,umol/ml), or glycerol (up to 10,umol/m ...
... nicotinic acid, I mg; p-amino benzoic acid, 0-1mg; pH 6.5. For growth experiments with glycerol the synthetic medium described by Bauchop & Elsden (1960) was used. Growthlimiting concentrations of glucose or fructose (up to 5,umol/ml), sodium lactate (up to 30 ,umol/ml), or glycerol (up to 10,umol/m ...
Insulin action on skeletal muscle protein metabolism during
... and cofactors involved in the proteolytic pathways correlated with results obtained in goats. The ATP-ubiquitin-proteasome dependent is, no doubt, a target for insulin in vivo. This does not preclude that insulin may have an inhibitory effect on muscle proteolysis by alternative mechanisms [24]. ...
... and cofactors involved in the proteolytic pathways correlated with results obtained in goats. The ATP-ubiquitin-proteasome dependent is, no doubt, a target for insulin in vivo. This does not preclude that insulin may have an inhibitory effect on muscle proteolysis by alternative mechanisms [24]. ...
AA-tRNA - Studentportalen
... I.) 43S complex includes 40S ribosomal subunit plus initiation factors eIF1, eIF1A, eIF3 and the eIF2*GTP*Met-tRNAi complex. It most probably includes eIF5 and eIF5B as well. eIF2 brings MettRNAi into the initiation complex. 2.) Formation of the complex between mRNA and the initiation factor IF4F. e ...
... I.) 43S complex includes 40S ribosomal subunit plus initiation factors eIF1, eIF1A, eIF3 and the eIF2*GTP*Met-tRNAi complex. It most probably includes eIF5 and eIF5B as well. eIF2 brings MettRNAi into the initiation complex. 2.) Formation of the complex between mRNA and the initiation factor IF4F. e ...
Identification and cDNA cloning of a Xenopus nucleolar
... mitosis by cdc2 kinase (Peter et al., 1990). Serine residues in acidic domains at the amino terminus are likely targets for CK II phosphorylation and TPXKK motifs in basic domains at the amino terminus are probable targets for cdc2 kinase phosphorylation. Phosphorylation of nucleolin by cdc2 kinase ...
... mitosis by cdc2 kinase (Peter et al., 1990). Serine residues in acidic domains at the amino terminus are likely targets for CK II phosphorylation and TPXKK motifs in basic domains at the amino terminus are probable targets for cdc2 kinase phosphorylation. Phosphorylation of nucleolin by cdc2 kinase ...
Short-Lived and Phosphorylated Proteins
... 24.2 KBq mL21; 3–8 nm) was added. Incubation was allowed to proceed for an additional 10 min, and then labeling was arrested with 1 mm unlabeled Leu. Cells were harvested by filtration and resuspended in 5 mL of ice-cold 1 m NaCl for 1 h to remove proteins entrapped in or loosely bound to the cell w ...
... 24.2 KBq mL21; 3–8 nm) was added. Incubation was allowed to proceed for an additional 10 min, and then labeling was arrested with 1 mm unlabeled Leu. Cells were harvested by filtration and resuspended in 5 mL of ice-cold 1 m NaCl for 1 h to remove proteins entrapped in or loosely bound to the cell w ...
Protein A Affinity Column for Monoclonal Antibody (MAb) Titer Analysis
... Staphylococcal protein A (SPA) plays an important role in immunology and biochemistry owing to its specific interaction with the Fc part of immunoglobulin G (IgG) from many mammals. SPA is a cell wall associated protein domain exposed on the surface of the Gram-positive bacterium, Staphylococcus aur ...
... Staphylococcal protein A (SPA) plays an important role in immunology and biochemistry owing to its specific interaction with the Fc part of immunoglobulin G (IgG) from many mammals. SPA is a cell wall associated protein domain exposed on the surface of the Gram-positive bacterium, Staphylococcus aur ...
- Wiley Online Library
... germline cells, we analyzed all 49 spots excised from the reference gel (GC gel) and spot 38, which was detected only in the VC gel, by mass spectrometry, and obtained MS/MS spectra for all 50 spots. Using the stringent standard of unused score not <4, we successfully identified proteins from 49 spo ...
... germline cells, we analyzed all 49 spots excised from the reference gel (GC gel) and spot 38, which was detected only in the VC gel, by mass spectrometry, and obtained MS/MS spectra for all 50 spots. Using the stringent standard of unused score not <4, we successfully identified proteins from 49 spo ...
The ErbB/HER receptor protein
... The ratio of protein phosphoserine/phosphothreonine/phosphotyrosine in normal animal cells is about 3000/300/1 [8]. Despite the paucity of protein-phosphotyrosines, they play key roles in signal transduction. Protein-serine/threonine kinases typically phosphorylate exogenous proteins, and this may b ...
... The ratio of protein phosphoserine/phosphothreonine/phosphotyrosine in normal animal cells is about 3000/300/1 [8]. Despite the paucity of protein-phosphotyrosines, they play key roles in signal transduction. Protein-serine/threonine kinases typically phosphorylate exogenous proteins, and this may b ...
The Case of Protein Kinase CK2
... first 20 N-terminal amino acids of the CK2β regulatory subunit are also involved in the interaction with Nopp140, a protein that binds a nuclear localization sequence and shuttles between the nucleus and the cytoplasm (Li et al., 1997). This part of the protein also contains two motifs that have bee ...
... first 20 N-terminal amino acids of the CK2β regulatory subunit are also involved in the interaction with Nopp140, a protein that binds a nuclear localization sequence and shuttles between the nucleus and the cytoplasm (Li et al., 1997). This part of the protein also contains two motifs that have bee ...
Phosphorylation
Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Phosphorylation and its counterpart, dephosphorylation, turn many protein enzymes on and off, thereby altering their function and activity. Protein phosphorylation is one type of post-translational modification.Protein phosphorylation in particular plays a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research (as of March 2015, the Medline database returns over 240,000 articles on the subject, largely on protein phosphorylation).