A Patch of Surface-Exposed Residues Mediates
... Protein kinases are frequent points of control in diverse signaling pathways, and their structure and enzymology are well understood (Huse and Kuriyan, 2002). There are four kinase substructures that control ATP binding and orientation, binding of the peptide substrate, and catalytic phosphotransfer ...
... Protein kinases are frequent points of control in diverse signaling pathways, and their structure and enzymology are well understood (Huse and Kuriyan, 2002). There are four kinase substructures that control ATP binding and orientation, binding of the peptide substrate, and catalytic phosphotransfer ...
The dynamics of plant plasma membrane proteins
... substrates for ER-associated protein degradation (ERAD), a ubiquitinmediated process in which misfolded or damaged ER proteins are retrotranslocated into the cytosol and degraded by the proteasome (Meusser et al., 2005). Homologs of yeast and mammalian ERAD components have been identified in the Ara ...
... substrates for ER-associated protein degradation (ERAD), a ubiquitinmediated process in which misfolded or damaged ER proteins are retrotranslocated into the cytosol and degraded by the proteasome (Meusser et al., 2005). Homologs of yeast and mammalian ERAD components have been identified in the Ara ...
Biochemistry 304 2014 Student Edition Gluconeogenesis Lectures
... one or more reactions unique to each pathway. a. Opposing pathways are regulated in a coordinated, reciprocal manner so that the stimulation of the anabolic pathway is accompanied by the inhibition of the catabolic one and vice versa. b. Biosynthetic pathways are typically regulated at an early, exe ...
... one or more reactions unique to each pathway. a. Opposing pathways are regulated in a coordinated, reciprocal manner so that the stimulation of the anabolic pathway is accompanied by the inhibition of the catabolic one and vice versa. b. Biosynthetic pathways are typically regulated at an early, exe ...
The Effect of Chemical Treatments of Albumin and Orosomucoid on
... results of one investigation may relate to a given protein in its monomeric form, and those of another may relate to it in some ill-defined aggregated form. Meaningful comparison of such results is impossible, since two such preparations of the same protein may each be taken up preferentially by a d ...
... results of one investigation may relate to a given protein in its monomeric form, and those of another may relate to it in some ill-defined aggregated form. Meaningful comparison of such results is impossible, since two such preparations of the same protein may each be taken up preferentially by a d ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... Electron microscopy after immunogold labeling with 11S globulin–specific antibodies revealed gold particles on the vesicles as well as on the protein storage vacuoles (Figure 1C), which contain large amounts of 11S globulin, the most abundant storage protein. Immunocytochemistry with 2S albumin–spec ...
... Electron microscopy after immunogold labeling with 11S globulin–specific antibodies revealed gold particles on the vesicles as well as on the protein storage vacuoles (Figure 1C), which contain large amounts of 11S globulin, the most abundant storage protein. Immunocytochemistry with 2S albumin–spec ...
Proteases and proteolytic cleavage of storage
... storage protein reactivation. The storage proteins must be made susceptible to proteolytic attack by appropriate structural changes, protease activation and changes in the localization of various components among various compartmentations. Limited proteolysis is essential for the initiation of stora ...
... storage protein reactivation. The storage proteins must be made susceptible to proteolytic attack by appropriate structural changes, protease activation and changes in the localization of various components among various compartmentations. Limited proteolysis is essential for the initiation of stora ...
Life in the slow lane: molecular mechanisms of estivation
... little de novo protein synthesis and reorganization of metabolism. One of the regulatory mechanisms that fits these requirements is reversible protein phosphorylation and in a variety of studies we have shown that this mechanism plays a key role in the regulation of multiple metabolic functions in e ...
... little de novo protein synthesis and reorganization of metabolism. One of the regulatory mechanisms that fits these requirements is reversible protein phosphorylation and in a variety of studies we have shown that this mechanism plays a key role in the regulation of multiple metabolic functions in e ...
[U-13C]propionate, phenylacetate, and acetaminophen
... urease and b-glucuronidase and then partially purified by ion-exchange chromatography before 13C NMR spectroscopy. Urine samples were adjusted to pH 7.0 and incubated overnight with 10,000 U of urease per sample. The pH was then readjusted to 7.0, and the urine was further incubated for 24 h at 37°C ...
... urease and b-glucuronidase and then partially purified by ion-exchange chromatography before 13C NMR spectroscopy. Urine samples were adjusted to pH 7.0 and incubated overnight with 10,000 U of urease per sample. The pH was then readjusted to 7.0, and the urine was further incubated for 24 h at 37°C ...
Cellular mechanisms regulating protein synthesis and skeletal
... protein synthesis. Currently, the mechanisms proposed in regulation of resistance exercise/contraction-induced compensatory growth include, but are not limited to, the involvement of local growth factors and the signaling events induced by the mechanical stimulus (39, 66, 96, 99). Previous studies h ...
... protein synthesis. Currently, the mechanisms proposed in regulation of resistance exercise/contraction-induced compensatory growth include, but are not limited to, the involvement of local growth factors and the signaling events induced by the mechanical stimulus (39, 66, 96, 99). Previous studies h ...
Linker histone H1 in early mouse embryogenesis
... protozoan, Tetrahymena, has a linker histone which contains only the C-terminal tail (reviewed by Wolffe et al., 1997). The C-terminal region is rich in basic amino acids, and it is likely that this tail domain interacts with negatively charged linker DNA to facilitate chromatin condensation (Ramakr ...
... protozoan, Tetrahymena, has a linker histone which contains only the C-terminal tail (reviewed by Wolffe et al., 1997). The C-terminal region is rich in basic amino acids, and it is likely that this tail domain interacts with negatively charged linker DNA to facilitate chromatin condensation (Ramakr ...
추가8
... Saccharomyces cerevisiae and the Crabtree effect S. cerevisiae is the only yeast that can produce ethanol and CO2 in such large quantities S. cerevisiae ferments carbohydrates efficiently and dominates its environment due to the Crabtree effect The Crabtree Effect Unlike most fermenting organis ...
... Saccharomyces cerevisiae and the Crabtree effect S. cerevisiae is the only yeast that can produce ethanol and CO2 in such large quantities S. cerevisiae ferments carbohydrates efficiently and dominates its environment due to the Crabtree effect The Crabtree Effect Unlike most fermenting organis ...
Adenosine triphosphate - Wikipedia, the free encyclopedia
... is ionized and exists mostly as ATP4−, with a small proportion of ATP3−.[18] As ATP has several negatively charged groups in neutral solution, it can chelate metals with very high affinity. The binding constant for various metal ions are (given as per mole) as Mg2+ (9 554), Na+ (13), Ca2+ (3 722), K ...
... is ionized and exists mostly as ATP4−, with a small proportion of ATP3−.[18] As ATP has several negatively charged groups in neutral solution, it can chelate metals with very high affinity. The binding constant for various metal ions are (given as per mole) as Mg2+ (9 554), Na+ (13), Ca2+ (3 722), K ...
Rheb and mammalian target of rapamycin in mitochondrial
... complex), which has GEF activity towards RagA/B [30,31]. If cells are starved of amino acids, RagA/B is in its GDP-bound state and RagC/D is in its GTP-bound state, which results in cytoplasmic localization of mTORC1. When cells are replenished with amino acids, RagC/D becomes GDP-bound and RagA/B b ...
... complex), which has GEF activity towards RagA/B [30,31]. If cells are starved of amino acids, RagA/B is in its GDP-bound state and RagC/D is in its GTP-bound state, which results in cytoplasmic localization of mTORC1. When cells are replenished with amino acids, RagC/D becomes GDP-bound and RagA/B b ...
Maltose uptake and its regulation in Bacillus subtilis
... glucose and maltose. In the absence of a high-energy phosphate donor, there was no detectable phosphorylation of either sugar. In the case of glucose, both ATP- and PEP-stimulated phosphorylation, demonstrating the presence of both kinase and PTS activity, respectively, for this substrate (Fig. IA). ...
... glucose and maltose. In the absence of a high-energy phosphate donor, there was no detectable phosphorylation of either sugar. In the case of glucose, both ATP- and PEP-stimulated phosphorylation, demonstrating the presence of both kinase and PTS activity, respectively, for this substrate (Fig. IA). ...
Glycolytic Enzymes Associate Dynamically with
... addition of KCN, an inhibitor of complex IV of the mitochondrial respiratory chain (Villani and Attardi, 2007), or increased by the addition of carbonyl cyanide m-chlorophenylhydrazone (CCCP), a proton ionophore that uncouples mitochondrial electron transport from ATP synthesis (Felle and Bentrup, 1 ...
... addition of KCN, an inhibitor of complex IV of the mitochondrial respiratory chain (Villani and Attardi, 2007), or increased by the addition of carbonyl cyanide m-chlorophenylhydrazone (CCCP), a proton ionophore that uncouples mitochondrial electron transport from ATP synthesis (Felle and Bentrup, 1 ...
20 Insulin Secretion and Action
... physiologic and biochemical studies have led to identification of regulatory sequence motifs along the insulin promoter and the binding proteins, such as islet-restricted proteins (BETA2, PDX-1, RIP3b1-Act/ C1) and ubiquitous proteins (E2A, HEB). Their DNAbinding activity and transactivating potency ...
... physiologic and biochemical studies have led to identification of regulatory sequence motifs along the insulin promoter and the binding proteins, such as islet-restricted proteins (BETA2, PDX-1, RIP3b1-Act/ C1) and ubiquitous proteins (E2A, HEB). Their DNAbinding activity and transactivating potency ...
non-RD - Crop Genetics Innovation
... perception in plants are commonly referred to as PTI (Pathogen Associated Molecular Pattern [PAMP] Triggered Immunity) and ETI (Effector Triggered Immunity), respectively [1]. Understanding how microbial signals are converted into PTI or ETI remains a fundamentally important issue. Most plant and an ...
... perception in plants are commonly referred to as PTI (Pathogen Associated Molecular Pattern [PAMP] Triggered Immunity) and ETI (Effector Triggered Immunity), respectively [1]. Understanding how microbial signals are converted into PTI or ETI remains a fundamentally important issue. Most plant and an ...
Mass Spectrometry Based Proteomics
... development and in the adult tissues in which they are co-expressed. Regulate the expression of insulin as well as proteins involved in glucose transport, glycolysis and mitochondrial metabolism, all of which are important in the regulation of insulin secretion . ...
... development and in the adult tissues in which they are co-expressed. Regulate the expression of insulin as well as proteins involved in glucose transport, glycolysis and mitochondrial metabolism, all of which are important in the regulation of insulin secretion . ...
PAI-1 - Trace: Tennessee Research and Creative Exchange
... broth with catabolite repression (SOC) media was added to each tube. This was followed by growth in optimal conditions (37°C and shaking at 250 rpm) for two hours. Tubes were then centrifuged for five minutes at 14,000 x g, and 400 µL of supernatant was removed leaving 100 µL for plating. Cell suspe ...
... broth with catabolite repression (SOC) media was added to each tube. This was followed by growth in optimal conditions (37°C and shaking at 250 rpm) for two hours. Tubes were then centrifuged for five minutes at 14,000 x g, and 400 µL of supernatant was removed leaving 100 µL for plating. Cell suspe ...
Bacillus subtilis serine/threonine protein kinase YabT is involved in
... subsequent substrate phosphorylation has been shown to contribute to spore germination (Shah et al., 2008). PrkD (also known as YbdM) is a soluble cytosolic protein. It was found to phosphorylate a two-component kinase DegS, and activate phosphotransfer from DegS to DegU (Jers et al., 2011). This in ...
... subsequent substrate phosphorylation has been shown to contribute to spore germination (Shah et al., 2008). PrkD (also known as YbdM) is a soluble cytosolic protein. It was found to phosphorylate a two-component kinase DegS, and activate phosphotransfer from DegS to DegU (Jers et al., 2011). This in ...
Chapter 25
... into the mitochondria where they are metabolized by -oxidation 8. Acetate produced in ethanol metabolism can be transformed into acetyl-CoA by the acetyl thiokinase reaction: Acetate + ATP + CoASH acetyl-CoA + AMP + PPi Acetyl-CoA then can enter the citric acid cycle and undergo oxidation to 2 CO ...
... into the mitochondria where they are metabolized by -oxidation 8. Acetate produced in ethanol metabolism can be transformed into acetyl-CoA by the acetyl thiokinase reaction: Acetate + ATP + CoASH acetyl-CoA + AMP + PPi Acetyl-CoA then can enter the citric acid cycle and undergo oxidation to 2 CO ...
Postprandial Serum Fructose Levels in Patients Tested
... Fructose enters hepatocytes where it is metabolized by fructokinase with consumption of ATP lactic acid and uric acid generated ...
... Fructose enters hepatocytes where it is metabolized by fructokinase with consumption of ATP lactic acid and uric acid generated ...
Research Interest
... Fructose enters hepatocytes where it is metabolized by fructokinase with consumption of ATP lactic acid and uric acid generated ...
... Fructose enters hepatocytes where it is metabolized by fructokinase with consumption of ATP lactic acid and uric acid generated ...
SURVEY AND SUMMARY H1 histones
... presented by Fang et al. support the formation of secondary structure, as the carboxy terminus of histone H1 settles into DNA helices, allowing for the formation of the nucleosome stem structure (49). Additionally, the interaction of the CTD with linker DNA has been shown to extend beyond the initia ...
... presented by Fang et al. support the formation of secondary structure, as the carboxy terminus of histone H1 settles into DNA helices, allowing for the formation of the nucleosome stem structure (49). Additionally, the interaction of the CTD with linker DNA has been shown to extend beyond the initia ...
Phosphate Groups Modifying Myelin Basic Proteins Are
... isolated myelin was followed for 1 too. Incorporation was maximal by 1 h, followed by a decay phase with a half-life of approximately 2 wk. However, radioactivity in the acid-soluble precursor pool (which always constituted at least half of the total radioactivity) decayed with a similar half-life, ...
... isolated myelin was followed for 1 too. Incorporation was maximal by 1 h, followed by a decay phase with a half-life of approximately 2 wk. However, radioactivity in the acid-soluble precursor pool (which always constituted at least half of the total radioactivity) decayed with a similar half-life, ...
Phosphorylation
Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Phosphorylation and its counterpart, dephosphorylation, turn many protein enzymes on and off, thereby altering their function and activity. Protein phosphorylation is one type of post-translational modification.Protein phosphorylation in particular plays a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research (as of March 2015, the Medline database returns over 240,000 articles on the subject, largely on protein phosphorylation).