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A fluxsensing mechanism could regulate the switch between
A fluxsensing mechanism could regulate the switch between

... as transcription factors) whose activity is modulated by their specific ligands. While for certain substrate molecules, we know such sensory systems [in yeast, e.g. the transmembrane receptors Snf3p and Rgt2p (Johnston & Kim, 2005)], or the intracellular receptor Gal3p (Sellick & Reece, 2005; Campbe ...
bioinformatics - Noble Research Lab
bioinformatics - Noble Research Lab

... These methods include the yeast two-hybrid screen and methods based on mass spectrometry (see von Mering et al., 2002 and references therein). The data obtained by these methods are partial: each experimental assay can identify only a subset of the interactions, and it has been estimated that for th ...
Insights into Chromatin Structure and Dynamics in Plants
Insights into Chromatin Structure and Dynamics in Plants

... 2.2.1. Histone Acetylation The best characterized post-translational modification regulating chromatin structure is the acetylation of one or more of the lysine residues in the histone N-terminal tails. Its regulatory role was proposed about 50 years ago [60]. Histone acetylation is thought to facil ...
Protective effects of sphingosine-1
Protective effects of sphingosine-1

... Aims Several experimental studies have demonstrated protection against cardiac ischaemia–reperfusion injury achieved by pre-treatment with exogenous sphingosine-1-phosphate (S1P). We tested the hypothesis that pharmacological S1P receptor agonists improve recovery of function when applied with reper ...
Characterization of the mineral phosphate solubilizing activity of
Characterization of the mineral phosphate solubilizing activity of

... Traditional production of P fertilizers is based on chemical processing of insoluble mineral phosphate high-grade ore, which includes an energy intensive treatment with sulfuric acid at high temperature. This process has become an environmentally undesirable and costly aVair (Vassilev et al. 2006). ...
Posttranslational Modification of MDM2
Posttranslational Modification of MDM2

... STAT5, and Ras/ERK pathways). In contrast, however, nuclear c-Abl plays a pivotal role in mediating apoptosis (39) and is phosphorylated and activated by the ATM protein kinase in response to genotoxic agents (40, 41). Part of the mechanism(s) by which nuclear c-Abl can induce apoptosis involves pho ...
Solubility-enhancing proteins MBP and NusA play a passive role in
Solubility-enhancing proteins MBP and NusA play a passive role in

... fusion proteins by TEV protease, GFP and DHFR, have biological activities that can be measured. We, therefore, sought to perform a more quantitative assessment of folding eYciency in these cases. To this end, the Xuorescence intensity of soluble extracts prepared from an equal number of cells produc ...
EFFECTS OF INSULIN DEFICIENCY ON EXERCISE
EFFECTS OF INSULIN DEFICIENCY ON EXERCISE

... the main nutrients. Its main function is to stimulate glucose uptake and disposal or utilization by the cells and thus to decrease blood glucose concentration. However, it also inhibits breakdown of proteins and lipids and promotes their synthesis. Type 1 diabetes is a disease in which insulin secre ...
Calcium, a signaling molecule in the endoplasmic reticulum?
Calcium, a signaling molecule in the endoplasmic reticulum?

... Ca21-dependent dynamics in the lumen of the ER. This diagram shows a model of events occurring in response to agonist-activated Ca21 fluctuations in the lumen of the endoplasmic reticulum (ER). These events affect the interactions between and the function of chaperones. (a) During agonist activation ...
Conservation of PHO pathway in ascomycetes and the role of Pho84
Conservation of PHO pathway in ascomycetes and the role of Pho84

... This degradation of low-affinity transporters, upon phosphate scarcity, serves as a positive feedback loop for upregulation of PHO pathway by further decreasing phosphate uptake by low-affinity transporters. However, the exact reason for this down-regulation of low-affinity transporters is not known ...
The structural biology of the amyloid precursor protein
The structural biology of the amyloid precursor protein

... 488      I. Coburger et al.: Multi-domain structure of APP Considering the function of the entire APP-protein and its involvement in the aetiology of AD, it is essential to know how these structural and functional segments form and act within the context of the entire protein and within the cellula ...
Ro52: Structure and interactions of constructs of RING and B-box
Ro52: Structure and interactions of constructs of RING and B-box

... The ubiquitination process is vital to maintain the protein homeostasis in the cell. With high specificity it regulates degradation of proteins by tagging them with a small protein called ubiquitin. Four proteins are involved to perform the process and in this thesis one of these proteins is studied ...
Functional Analysis of Subunit e of the F1Fo
Functional Analysis of Subunit e of the F1Fo

... the Su e⌬C50HA and Su e⌬C60HA derivatives, respectively (Fig. 1B). Thus, the most extreme deletion, the Su e⌬C60HA derivative, is composed of only the initial 36 amino acid residues of Su e, a region which encompasses the N-terminal hydrophobic membrane anchor. The truncated derivatives were express ...
Control de la estabilidad de ciclinas de G1 por nutrientes Sara
Control de la estabilidad de ciclinas de G1 por nutrientes Sara

... hen environmental conditions change, Saccharomyces cerevisiae, like other organisms, takes several coordinated decisions about growth, cell division, and maintaining homeostasis. Nutrient status is among the most important environmental conditions that must be accurately sensed and responded to in o ...
Cell signalling - The Open University
Cell signalling - The Open University

... pathways in different cell types;  the signal can be amplified (or damped down) as it travels along the signalling pathway;  it can switch on multiple pathways, leading to several cellular responses in diverse regions of the cell;  information can be processed from several different receptors at ...
M6PRs are found in a subset of PC12 cell ISGs
M6PRs are found in a subset of PC12 cell ISGs

... 1981). Subsequent studies have added to the morphological definition of this compartment in a variety of cell types (for review see Tooze, 1991; Arvan and Castle, 1998). The consensus from morphological and more recent biochemical ...
Lecture 9 – Cellular Respiration
Lecture 9 – Cellular Respiration

... breakdown of all carbohydrates to disaccharides • Maltases, lactases, and sucrases break down disaccharides into monosaccharides • Glucose is brought to all the cells in the body through the circulatory system NSCC BIOL211 ...
A brief history of ATP
A brief history of ATP

... motors of life spinning around in our bodies.’’ The spinning ATP synthase enzyme that coins ATP from its precursor, ADP, is found in rod-like structures called mitochondria that lurk in our cells. Every mitochondrion is studded with hundreds if not thousands of the enzymes. Unlock the secrets of ATP ...
Cellular Respiration
Cellular Respiration

... http://get.adobe.com/flashplayer. ...
Changes in Typical Organelles in Developing Cotyledons of Soybean
Changes in Typical Organelles in Developing Cotyledons of Soybean

... were also recognized (Figs. 3C, 30) . These organelles were distinctly stained by PAS, CBB and osmium and could also be distinguished from other structures by their spherical shape . Their number and size increased around 30 OAF. Until they are better understood, we are calling these organelles prot ...
Protein Folding at the Exit Tunnel
Protein Folding at the Exit Tunnel

... unfolded state (see below), molecular chaperones, the ribosome, a highly crowded medium (200300 mg/ml total protein concentration), co-factors such as heme, NADH and others, intracellular processes such as posttranslational modifications and quality-control processes such as protein degradation. In ...
- Opus
- Opus

... Skeletal  muscle  glycogen  provides  a  rapid  and  efficient  (energy  yield  per  unit  oxygen)  fuel  source for energy expenditure, such that when skeletal muscle glycogen stores are depleted, the rate  of energy production is severely compromised. Clear support for the important role of glycog ...
Understanding the acetylome: translating targeted
Understanding the acetylome: translating targeted

... protein acetylation became strikingly apparent following the recent characterization of the “acetylome” by Matthias Mann’s group (12). Utilizing acetylation-based immunoprecipitation to enrich acetylated peptides from MV4-11 cells (a human acute myeloid leukemia cell line), followed by high-resoluti ...
Plant and Soil
Plant and Soil

... fumarate (Table 5). The bacteroids of the ineffective C4-dicarboxylic acid utilization mutant P121S21 did not oxidize any of the carbon sources tested. The respective plant cytosol fractions, stimulated the respiration of P121 bacteroids but had not any effect on P121S21 bacteroids. Our results are ...
The Generic Nature of Protein Folding and Misfolding
The Generic Nature of Protein Folding and Misfolding

... core of the folding nucleus; in essence, these interactions force the chain to adopt a rudimentary native-like architecture (Vendruscolo et al., 2001, Lindorff-Larsen et al., 2004; Vendruscolo and Dobson, 2005). Once this topology has been achieved, the native structure is almost invariably generate ...
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Phosphorylation



Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Phosphorylation and its counterpart, dephosphorylation, turn many protein enzymes on and off, thereby altering their function and activity. Protein phosphorylation is one type of post-translational modification.Protein phosphorylation in particular plays a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research (as of March 2015, the Medline database returns over 240,000 articles on the subject, largely on protein phosphorylation).
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