Infectious Salmon Anaemia Virus (ISAV) RNA Binding Protein
... to viral nucleic acids detected by pattern recognition receptors (PRRs) and lead to transcription of interferon stimulated genes (ISGs) [10]. ISG15 is an ubiquitin-like protein modifier that is strongly induced by type I IFN (review in [11]), and it targets viral proteins from various virus families ...
... to viral nucleic acids detected by pattern recognition receptors (PRRs) and lead to transcription of interferon stimulated genes (ISGs) [10]. ISG15 is an ubiquitin-like protein modifier that is strongly induced by type I IFN (review in [11]), and it targets viral proteins from various virus families ...
Chromatin and the genome integrity network
... during the later DNA-processing events of homologous recombination9. The functional importance of these chromatinremodelling events is less clear. In one case in S. cerevisiae, RSC was reported to have a key role in the recruitment of early damage responders, such as the yeast MRX complex (a heterot ...
... during the later DNA-processing events of homologous recombination9. The functional importance of these chromatinremodelling events is less clear. In one case in S. cerevisiae, RSC was reported to have a key role in the recruitment of early damage responders, such as the yeast MRX complex (a heterot ...
2015 – PKc-theta is a novel sc35 splicing factor regulator in
... relevant genes undergo alternative splicing, the role of alternative splicing in T cell memory remains largely unexplored. SC35 (also known as SRSF2) is a well-characterized splicing factor that belongs to the serine/arginine-rich (SR) protein family, an important class of splicing regulators. SR pr ...
... relevant genes undergo alternative splicing, the role of alternative splicing in T cell memory remains largely unexplored. SC35 (also known as SRSF2) is a well-characterized splicing factor that belongs to the serine/arginine-rich (SR) protein family, an important class of splicing regulators. SR pr ...
Regulation of chromatin by histone modifications
... levels of poly-ADP ribosylated histones that have been correlated with a relatively relaxed chromatin state [38]. Presumably, this is a consequence, at least in part, of the negative charge that the modification confers to the histone. In addition, though, it has been reported that the activation of ...
... levels of poly-ADP ribosylated histones that have been correlated with a relatively relaxed chromatin state [38]. Presumably, this is a consequence, at least in part, of the negative charge that the modification confers to the histone. In addition, though, it has been reported that the activation of ...
Quantitative Host Cell Protein Analysis Using Two
... lot-release, it does not provide any information on the profile or properties of HCPs present which may be used for process design improvement. Hence methods that facilitate identification of individual HCPs have gained prominence in recent years, e.g., HCPs present in products produced by different CH ...
... lot-release, it does not provide any information on the profile or properties of HCPs present which may be used for process design improvement. Hence methods that facilitate identification of individual HCPs have gained prominence in recent years, e.g., HCPs present in products produced by different CH ...
selection of antigens for antibody-based proteomics
... susceptible to degradation, and therefore careful handling of samples is required to not lower already weak protein signals further [Falk et al. 2007]. There is no equivalent to the polymerase chain reaction (PCR) for proteins, and without enrichment, the low signals are only detectable by a few sen ...
... susceptible to degradation, and therefore careful handling of samples is required to not lower already weak protein signals further [Falk et al. 2007]. There is no equivalent to the polymerase chain reaction (PCR) for proteins, and without enrichment, the low signals are only detectable by a few sen ...
Stimulatory and Inhibitory Functions of the R Domain
... ∆NEG2-CFTR still contains all 10 PKA phosphorylation sites. The synthetic 22-amino acid NEG2 peptide interacts with the CFTR molecule and exhibits both stimulatory and inhibitory effects on CFTR function (Fig. 4B). Additionally, covalent modification of a cystine residue at position 832, which resid ...
... ∆NEG2-CFTR still contains all 10 PKA phosphorylation sites. The synthetic 22-amino acid NEG2 peptide interacts with the CFTR molecule and exhibits both stimulatory and inhibitory effects on CFTR function (Fig. 4B). Additionally, covalent modification of a cystine residue at position 832, which resid ...
Cellular Respiration and Fermentation
... why many different molecules—including lipids, amino acids, and CO2-end up as radiolabeled when cells are fed glucose with radioactive carbons (14C). Metabolism comprises thousands of different chemical reactions, yet the amounts and identities of molecules inside cells are relatively constant. By r ...
... why many different molecules—including lipids, amino acids, and CO2-end up as radiolabeled when cells are fed glucose with radioactive carbons (14C). Metabolism comprises thousands of different chemical reactions, yet the amounts and identities of molecules inside cells are relatively constant. By r ...
Increased expression of the F1Fo ATP synthase in response to iron
... activity, ATP depletion appears to be related to the increased expression of these F1 subunits. The apoptosis induced by oligomycin in erythroleukemia cells points to a critical role of F1Fo ATP synthase in determining cell fate by reducing ATP levels (13). Similarly, overexpression of the α and β s ...
... activity, ATP depletion appears to be related to the increased expression of these F1 subunits. The apoptosis induced by oligomycin in erythroleukemia cells points to a critical role of F1Fo ATP synthase in determining cell fate by reducing ATP levels (13). Similarly, overexpression of the α and β s ...
Amyloid deposits - Lindquist Lab
... A host of studies involving proteins linked to neurodegenerative diseases and other amyloidogenic proteins have investigated the role of inclusion and plaque formation in pathogenicity. The case is perhaps strongest for Huntington disease, for which it has been postulated that inclusions cause toxic ...
... A host of studies involving proteins linked to neurodegenerative diseases and other amyloidogenic proteins have investigated the role of inclusion and plaque formation in pathogenicity. The case is perhaps strongest for Huntington disease, for which it has been postulated that inclusions cause toxic ...
Molecular mapping of tyrosine-phosphorylated proteins in focal
... plays a pivotal role in the regulation of tissue integrity, cell migration and transmembrane signaling. These adhesions are formed in specialized sub-cellular sites, known as focal adhesions (Burridge and Chrzanowska-Wodnicka, 1996; Geiger et al., 2001) where matrix-attached integrin receptors inter ...
... plays a pivotal role in the regulation of tissue integrity, cell migration and transmembrane signaling. These adhesions are formed in specialized sub-cellular sites, known as focal adhesions (Burridge and Chrzanowska-Wodnicka, 1996; Geiger et al., 2001) where matrix-attached integrin receptors inter ...
PDF
... down HA-tagged PP4 when co-expressed in S2 cells (see Fig. S3A, lanes 2, 5 and 6, top panel in the supplementary material), suggesting that amino acids 626-678 of Smo is responsible for either direct or indirect association between SmoCT and PP4. We reasoned that if PP4 inhibits Smo phosphorylation ...
... down HA-tagged PP4 when co-expressed in S2 cells (see Fig. S3A, lanes 2, 5 and 6, top panel in the supplementary material), suggesting that amino acids 626-678 of Smo is responsible for either direct or indirect association between SmoCT and PP4. We reasoned that if PP4 inhibits Smo phosphorylation ...
Pleiotropy of leptin receptor signalling is defined by distinct roles of
... activation of the RAS ⁄ RAF ⁄ ERK pathway [26,27]. In contrast to Tyr985 and Tyr1138, the role of Tyr1077 in leptin signalling is not yet clear. More recently, it has been shown that AMP-dependent protein kinase (AMPK) appears to be a downstream mediator of leptin signalling. Leptin directly stimula ...
... activation of the RAS ⁄ RAF ⁄ ERK pathway [26,27]. In contrast to Tyr985 and Tyr1138, the role of Tyr1077 in leptin signalling is not yet clear. More recently, it has been shown that AMP-dependent protein kinase (AMPK) appears to be a downstream mediator of leptin signalling. Leptin directly stimula ...
Metabolic rate depression in animals
... becomes intermittent, and kidney filtration rate is reduced. Organisms do not eat so the energetic costs of digestion, nutrient absorption, and peristalsis are eliminated. A substantial part of total energy savings comes from the suppression of these physiological activities. Metabolic rate is also i ...
... becomes intermittent, and kidney filtration rate is reduced. Organisms do not eat so the energetic costs of digestion, nutrient absorption, and peristalsis are eliminated. A substantial part of total energy savings comes from the suppression of these physiological activities. Metabolic rate is also i ...
Metabolic flux profiling of recombinant protein secreting Pichia
... Pichia pastoris is an attractive system for the production of recombinant proteins [1–4]. Moreover, the development of systems biotechnology tools specific for this cell factory [5–10] has opened new opportunities for strain improvement and rational design of culture conditions. Several studies ...
... Pichia pastoris is an attractive system for the production of recombinant proteins [1–4]. Moreover, the development of systems biotechnology tools specific for this cell factory [5–10] has opened new opportunities for strain improvement and rational design of culture conditions. Several studies ...
Identification, Purification, and Molecular Cloning of
... by us can be explained by the application of the doublelayer silicone–oil–filtration–centrifugation technique, which allows assay times of ⵑ1 sec or less. Short assay times are required for transport systems that attain equilibrium rapidly. The shortest assay times used by Schäfer et al. (1977) were ...
... by us can be explained by the application of the doublelayer silicone–oil–filtration–centrifugation technique, which allows assay times of ⵑ1 sec or less. Short assay times are required for transport systems that attain equilibrium rapidly. The shortest assay times used by Schäfer et al. (1977) were ...
as PDF
... ATP depletion in cells, or low blood glucose level, lead to the activation of glycogenolysis and the enhancement of glucose degradation through glycolysis. Glycolysis is a central metabolic pathway of glucose metabolism, starting with glucose-6-phosphate, produced by glycogenolysis or gluconeogenesi ...
... ATP depletion in cells, or low blood glucose level, lead to the activation of glycogenolysis and the enhancement of glucose degradation through glycolysis. Glycolysis is a central metabolic pathway of glucose metabolism, starting with glucose-6-phosphate, produced by glycogenolysis or gluconeogenesi ...
Chapter 8 Cellular Respiration
... of H ions used to produce ATP – Of the 36 molecules of ATP produced from one molecule of glucose ...
... of H ions used to produce ATP – Of the 36 molecules of ATP produced from one molecule of glucose ...
A human homologue of yeast anti-silencing factor has histone
... factor A) functions as a histone chaperone in an ATPindependent manner. Other histone chaperones reported to date have in common acidic regions, which are likely to be involved in both binding to histones and nucleosome assembly activity (Kleinschmidt & Franke 1982; Kawase et al. 1996; Ito et al. 19 ...
... factor A) functions as a histone chaperone in an ATPindependent manner. Other histone chaperones reported to date have in common acidic regions, which are likely to be involved in both binding to histones and nucleosome assembly activity (Kleinschmidt & Franke 1982; Kawase et al. 1996; Ito et al. 19 ...
SUGAR SENSING AND SIGNALING IN PLANTS
... concomitant production of oxygen. Consistent with their importance as the prime carbon and energy sources for most cell types, sugars, in addition, have acquired important regulatory functions early in evolution, controlling metabolism, stress resistance, growth, and development in bacteria, yeasts, ...
... concomitant production of oxygen. Consistent with their importance as the prime carbon and energy sources for most cell types, sugars, in addition, have acquired important regulatory functions early in evolution, controlling metabolism, stress resistance, growth, and development in bacteria, yeasts, ...
In vivo assays to study histone ubiquitylation
... methylation of adjacent lysine residues in the H3 N tail by several SET domain proteins [40–43]. These results are not consistent with a model in which histone ubiquitylation merely serves a structural role to open chromatin. Ubiquitylation of proteins destined for degradation occurs through a conce ...
... methylation of adjacent lysine residues in the H3 N tail by several SET domain proteins [40–43]. These results are not consistent with a model in which histone ubiquitylation merely serves a structural role to open chromatin. Ubiquitylation of proteins destined for degradation occurs through a conce ...
Chapter 9: Pathways that Harvest Chemical
... This is an oxidation-reduction reaction. Glucose (C6H12O6) becomes completely oxidized and six molecules of O2 are reduced to six molecules of water. The energy that is released can be used to do work. The same equation applies to the overall metabolism of glucose in cells. However, in contrast to c ...
... This is an oxidation-reduction reaction. Glucose (C6H12O6) becomes completely oxidized and six molecules of O2 are reduced to six molecules of water. The energy that is released can be used to do work. The same equation applies to the overall metabolism of glucose in cells. However, in contrast to c ...
Functional Anthology of Intrinsic Disorder. 1. Biological Processes
... rank the significance of these correlations. Protein sequence data redundancy and the relationship between protein length and protein structure were taken into consideration to ensure the quality of the statistical inferences. Over 200 000 proteins from the Swiss-Prot database were analyzed using th ...
... rank the significance of these correlations. Protein sequence data redundancy and the relationship between protein length and protein structure were taken into consideration to ensure the quality of the statistical inferences. Over 200 000 proteins from the Swiss-Prot database were analyzed using th ...
Slow Protein Conformational Change, Allostery and
... In this model, the rate of product formation increases along with the substrate concentration [S] with the characteristic hyperbolic relationship, asymptotically approaching its maximum rate Vmax = k[E]t, ([E]t is the total enzyme concentration) attained when all enzymes are bound to substrates .We ...
... In this model, the rate of product formation increases along with the substrate concentration [S] with the characteristic hyperbolic relationship, asymptotically approaching its maximum rate Vmax = k[E]t, ([E]t is the total enzyme concentration) attained when all enzymes are bound to substrates .We ...
TIBS Review - Structural Genomics Consortium
... deregulation in disease. Here we review recent advancements in our understanding of BET proteins, their structural organization and their involvement in disease as well as the translation of their small molecule inhibitors to the clinic. ...
... deregulation in disease. Here we review recent advancements in our understanding of BET proteins, their structural organization and their involvement in disease as well as the translation of their small molecule inhibitors to the clinic. ...
Phosphorylation
Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Phosphorylation and its counterpart, dephosphorylation, turn many protein enzymes on and off, thereby altering their function and activity. Protein phosphorylation is one type of post-translational modification.Protein phosphorylation in particular plays a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research (as of March 2015, the Medline database returns over 240,000 articles on the subject, largely on protein phosphorylation).