Multicellular trichomes in Arabidopsis - Development
... and co-workers (Hemerly et al., 1995) introduced a dominantnegative mutant of a mitotic cyclin-dependent kinase gene into transgenic tobacco plants, which resulted in plants with essentially normal morphology, but fewer and larger cells. In another study, overexpression of a mitotic B-cyclin in Arab ...
... and co-workers (Hemerly et al., 1995) introduced a dominantnegative mutant of a mitotic cyclin-dependent kinase gene into transgenic tobacco plants, which resulted in plants with essentially normal morphology, but fewer and larger cells. In another study, overexpression of a mitotic B-cyclin in Arab ...
Effects of various oxidants and antioxidants on the p38
... We investigated the effects of different antioxidants such as L-ascorbic acid, catalase, and superoxide dismutase (SOD), on the p38-MAPK activation induced by oxidative stress in the isolated perfused amphibian heart. Oxidative stress was exemplified by perfusing hearts with 30 μM H2 O2 for 5 min or ...
... We investigated the effects of different antioxidants such as L-ascorbic acid, catalase, and superoxide dismutase (SOD), on the p38-MAPK activation induced by oxidative stress in the isolated perfused amphibian heart. Oxidative stress was exemplified by perfusing hearts with 30 μM H2 O2 for 5 min or ...
The novel mouse mutant, chuzhoi, has disruption of Ptk7
... mutations in only eleven genes leads to craniorachischisis, which makes the identification of novel mutants with this disorder of high scientific and clinical interest. Strikingly, amongst those mutants with craniorachischisis, almost all genes affect the planar cell polarity (PCP) signalling pathwa ...
... mutations in only eleven genes leads to craniorachischisis, which makes the identification of novel mutants with this disorder of high scientific and clinical interest. Strikingly, amongst those mutants with craniorachischisis, almost all genes affect the planar cell polarity (PCP) signalling pathwa ...
Ion Trap/Ion Mobility/Quadrupole/Time-of
... with a Q/TOF spectrometer. We have previously reported an IMSTOF approach;4 substantial differences in the time scale required for each separation (milliseconds in the high-pressure drift tube, compared to microseconds in the evacuated TOF instrument) allow mobilities and m/z ratios for mixtures of ...
... with a Q/TOF spectrometer. We have previously reported an IMSTOF approach;4 substantial differences in the time scale required for each separation (milliseconds in the high-pressure drift tube, compared to microseconds in the evacuated TOF instrument) allow mobilities and m/z ratios for mixtures of ...
Calretinin
... EF Hands • The Ca2+ ion binds to the loop connecting the two helices. • In this loop, there are amino acid residues with negatively charged oxygen atoms, which attract the positively charged calcium ion • When Ca2+ enters the cell Calretinin binds about 99% of it. ...
... EF Hands • The Ca2+ ion binds to the loop connecting the two helices. • In this loop, there are amino acid residues with negatively charged oxygen atoms, which attract the positively charged calcium ion • When Ca2+ enters the cell Calretinin binds about 99% of it. ...
Bacterial tail anchors can target to the mitochondrial outer
... If conversion of endosymbiont to mitochondria were the rare and essential event ...
... If conversion of endosymbiont to mitochondria were the rare and essential event ...
PDF - Potter Lab
... To determine whether Tsc1 affected cell and organ size during development, we generated large Tsc1 mutant clones in the eye and wing discs and found that mosaic discs were two to three times larger than wild-type controls (Figures 2A and 2B, data not shown). Phalloidin staining revealed that the Tsc ...
... To determine whether Tsc1 affected cell and organ size during development, we generated large Tsc1 mutant clones in the eye and wing discs and found that mosaic discs were two to three times larger than wild-type controls (Figures 2A and 2B, data not shown). Phalloidin staining revealed that the Tsc ...
The lysosome as a command-and-control center for cellular
... that housed a pool of soluble hydrolases capable of degrading proteins, nucleic acids, carbohydrates, lipids, and cellular debris. Because of these easily detectable activities, the lysosome quickly earned its reputation as the cell’s “trash can” or “recycle bin.” There are multiple routes via which ...
... that housed a pool of soluble hydrolases capable of degrading proteins, nucleic acids, carbohydrates, lipids, and cellular debris. Because of these easily detectable activities, the lysosome quickly earned its reputation as the cell’s “trash can” or “recycle bin.” There are multiple routes via which ...
ASH1 by Puf6p–Fun12p/eIF5B interaction and released by CK2 phosphorylation Yingfeng Deng,
... al. 2000), which contains putative Puf6p-binding sites at the 3⬘UTR (Gu et al. 2004). IST2 mRNA was found diffusely distributed within the bud in both fun12 and puf6 mutants (Supplemental Fig. 4), similar to the pattern observed for ASH1 mRNA. Both the N-terminal region and PUF domain of Puf6p are r ...
... al. 2000), which contains putative Puf6p-binding sites at the 3⬘UTR (Gu et al. 2004). IST2 mRNA was found diffusely distributed within the bud in both fun12 and puf6 mutants (Supplemental Fig. 4), similar to the pattern observed for ASH1 mRNA. Both the N-terminal region and PUF domain of Puf6p are r ...
COMPLEX FORMATION AND PROTEIN INTERACTION IN THE
... called terminal complexes, in cells of higher plants through freeze-fracture electron microscopy (Mueller et al. 1976). These terminal complexes were later identified as forming a hexameric “rosette” structure in the plasma membrane and named the Cellulose Synthesis Complex (CSC) (Mueller & Brown 19 ...
... called terminal complexes, in cells of higher plants through freeze-fracture electron microscopy (Mueller et al. 1976). These terminal complexes were later identified as forming a hexameric “rosette” structure in the plasma membrane and named the Cellulose Synthesis Complex (CSC) (Mueller & Brown 19 ...
Characterization of Lipid Rafts from Medicago
... was also performed from Arabidopsis (Arabidopsis thaliana) callus microsomes (Borner et al., 2005). Other studies have identified proteins present in DIMs of Arabidopsis leaves and the tobacco (Nicotiana tabacum) Bright-Yellow2 (BY2) cell line (Peskan et al., 2000; Shahollari et al., 2004; Morel et ...
... was also performed from Arabidopsis (Arabidopsis thaliana) callus microsomes (Borner et al., 2005). Other studies have identified proteins present in DIMs of Arabidopsis leaves and the tobacco (Nicotiana tabacum) Bright-Yellow2 (BY2) cell line (Peskan et al., 2000; Shahollari et al., 2004; Morel et ...
Ubiquitin ligases and beyond EDITORIAL Open Access Ivan Dikic
... determines the substrate specificity of ubiquitination, and the diversity of the cellular functions of ubiquitination is reflected in the existence of some hundreds of different mammalian E3s, compared with a few dozen E2s and two E1s. The E2 conjugating enzymes have special significance in determin ...
... determines the substrate specificity of ubiquitination, and the diversity of the cellular functions of ubiquitination is reflected in the existence of some hundreds of different mammalian E3s, compared with a few dozen E2s and two E1s. The E2 conjugating enzymes have special significance in determin ...
The lysosome as a command-and-control center for cellular
... that housed a pool of soluble hydrolases capable of degrading proteins, nucleic acids, carbohydrates, lipids, and cellular debris. Because of these easily detectable activities, the lysosome quickly earned its reputation as the cell’s “trash can” or “recycle bin.” There are multiple routes via which ...
... that housed a pool of soluble hydrolases capable of degrading proteins, nucleic acids, carbohydrates, lipids, and cellular debris. Because of these easily detectable activities, the lysosome quickly earned its reputation as the cell’s “trash can” or “recycle bin.” There are multiple routes via which ...
Ribosylation of bovine serum albumin induces ROS accumulation
... (0–40 lM) for 24 h. The culture medium was then changed to DMEM containing 10 % fetal bovine serum. MTT (final concentration 0.5 mg/ml; 50 ll) was added after adding the glycated protein. Plates were incubated at 37 °C for 4 h, then the assay was stopped by replacement of the MTT-containing medium w ...
... (0–40 lM) for 24 h. The culture medium was then changed to DMEM containing 10 % fetal bovine serum. MTT (final concentration 0.5 mg/ml; 50 ll) was added after adding the glycated protein. Plates were incubated at 37 °C for 4 h, then the assay was stopped by replacement of the MTT-containing medium w ...
Arbuscular mycorrhiza: the mother of plant root endosymbioses
... is currently much interest in the molecular identification of fungal signalling molecules that induce symbiosis-specific responses in the host root (collectively called Myc factors). The existence of such molecules became apparent in experiments in which direct contact between the fungus and plant w ...
... is currently much interest in the molecular identification of fungal signalling molecules that induce symbiosis-specific responses in the host root (collectively called Myc factors). The existence of such molecules became apparent in experiments in which direct contact between the fungus and plant w ...
Actin Filaments Play a Critical Role in Vacuolar
... suggests that transiently expressed actin2[D13K] and actin2[D13Q] disrupt endogenous actin filaments. Approximately 46% of the protoplasts transformed with actin2[D13K] showed a diffuse staining pattern of GFP:talin (Figure 3B). The expression of the introduced actin2 proteins was confirmed by prote ...
... suggests that transiently expressed actin2[D13K] and actin2[D13Q] disrupt endogenous actin filaments. Approximately 46% of the protoplasts transformed with actin2[D13K] showed a diffuse staining pattern of GFP:talin (Figure 3B). The expression of the introduced actin2 proteins was confirmed by prote ...
+Tec - Blood Journal
... [‘*P]orthophosphate labeling, 32D cells (1 X IO’lexperiment) were cultured in phosphate-free RPM1 1640 medium (GIBCO) supplemented with 37 MBqlmL of [”Plorthophosphate (Amersham) without IL-3 or FCS for 2 hours and then stimulated with IL-3 for 5 minutes. Tec was immunoprecipitated from the lysates ...
... [‘*P]orthophosphate labeling, 32D cells (1 X IO’lexperiment) were cultured in phosphate-free RPM1 1640 medium (GIBCO) supplemented with 37 MBqlmL of [”Plorthophosphate (Amersham) without IL-3 or FCS for 2 hours and then stimulated with IL-3 for 5 minutes. Tec was immunoprecipitated from the lysates ...
atlas of dengue viruses morphology and morphogenesis
... Electron microscopy has been used as a very efficient tool to study virus particle morphology (Ackermann & Berthiaume, 1995; Doane & Anderson, 1987; Madeley & Field, 1988), as the unique technique for direct visualization of morphological structures. Virus infected tissues can also be analyzed by th ...
... Electron microscopy has been used as a very efficient tool to study virus particle morphology (Ackermann & Berthiaume, 1995; Doane & Anderson, 1987; Madeley & Field, 1988), as the unique technique for direct visualization of morphological structures. Virus infected tissues can also be analyzed by th ...
Sample pages 1 PDF
... barbed end (see Chapter). The individual class of myosin moves unidirectionally along an actin filament, and the most of myosin classes thus far move toward the barbed end, with an exception of myosin VI moving toward the pointed end [110]. Similarly to myosin II and myosin V, the tobacco 175-kDa my ...
... barbed end (see Chapter). The individual class of myosin moves unidirectionally along an actin filament, and the most of myosin classes thus far move toward the barbed end, with an exception of myosin VI moving toward the pointed end [110]. Similarly to myosin II and myosin V, the tobacco 175-kDa my ...
EGF receptor signaling pathway Drosophila activation
... motif (SAM) and conserved region in Cnk (CRIC) domains, promotes Raf activation downstream of Ras, while its C-terminal region, which contains a Raf-binding domain, plays an inhibitory role downstream of Raf (Therrien et al. 1999; Douziech et al. 2003). It has been proposed that Src42 binding antago ...
... motif (SAM) and conserved region in Cnk (CRIC) domains, promotes Raf activation downstream of Ras, while its C-terminal region, which contains a Raf-binding domain, plays an inhibitory role downstream of Raf (Therrien et al. 1999; Douziech et al. 2003). It has been proposed that Src42 binding antago ...
Full Text - Molecular Cancer Research
... entry from G1 to S and intra-S progression, whereas all 3 CDC25 genes function in G2–M progression (16). The developmental roles of Cdc25 have been partially defined. In mice, Cdc25A is essential for embryonic development. Cdc25A/ embryos are resorbed at around E6.5 due to widespread apoptosis (17) ...
... entry from G1 to S and intra-S progression, whereas all 3 CDC25 genes function in G2–M progression (16). The developmental roles of Cdc25 have been partially defined. In mice, Cdc25A is essential for embryonic development. Cdc25A/ embryos are resorbed at around E6.5 due to widespread apoptosis (17) ...
DNA REPLICATION CHECKPOINT CONTROL 7. Acknowledgments
... importantly, the integrity of the genome must be ensured before it is passed to daughter cells. In unicellular organisms such as yeast, loss of genetic material may lead to cell death. However, in higher eukaryotes genomic instability is causally related with tumorigenesis. To combat this instabilit ...
... importantly, the integrity of the genome must be ensured before it is passed to daughter cells. In unicellular organisms such as yeast, loss of genetic material may lead to cell death. However, in higher eukaryotes genomic instability is causally related with tumorigenesis. To combat this instabilit ...
PDF
... development during oogenesis. The most striking phenotype observed for all three genes is a loss of apicalbasal polarity and accumulation of follicular epithelial cells in multiple layers around the oocyte. The spatiotemporal onset of this adenoma-like phenotype correlates with the ...
... development during oogenesis. The most striking phenotype observed for all three genes is a loss of apicalbasal polarity and accumulation of follicular epithelial cells in multiple layers around the oocyte. The spatiotemporal onset of this adenoma-like phenotype correlates with the ...
Lessons on longevity from budding yeast
... able to retain most of the age-associated damage3,4. In the chronological model, by contrast, damage accumulates over time within a nondividing cell until a threshold is reached beyond which the cell is no longer able to re-enter the cell cycle (Fig. 1). Despite these differences, there are some int ...
... able to retain most of the age-associated damage3,4. In the chronological model, by contrast, damage accumulates over time within a nondividing cell until a threshold is reached beyond which the cell is no longer able to re-enter the cell cycle (Fig. 1). Despite these differences, there are some int ...
Plant tropisms - Semantic Scholar
... elongating at a greater rate in one region of the responding organ relative to an opposing position within that same organ. As most work in the area of tropic response has shown, curvature can only be properly manifested through the coordinated activity of hormones. Small fluctuations in the cellula ...
... elongating at a greater rate in one region of the responding organ relative to an opposing position within that same organ. As most work in the area of tropic response has shown, curvature can only be properly manifested through the coordinated activity of hormones. Small fluctuations in the cellula ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.