COMMUNICATION Redefining the phenotype of Heat shock protein 90
... Herein we show that the phenotype of Hsp90 knockdown cells using CRISPR technology is significantly different to that generated when cells are treated with classical inhibitors. We reveal that drugs acting via mechanisms unrelated to Hsp90 induce a similar phenotype to that produced by classical Hsp ...
... Herein we show that the phenotype of Hsp90 knockdown cells using CRISPR technology is significantly different to that generated when cells are treated with classical inhibitors. We reveal that drugs acting via mechanisms unrelated to Hsp90 induce a similar phenotype to that produced by classical Hsp ...
(Hsp90) inhibitors
... Herein we show that the phenotype of Hsp90 knockdown cells using CRISPR technology is significantly different to that generated when cells are treated with classical inhibitors. We reveal that drugs acting via mechanisms unrelated to Hsp90 induce a similar phenotype to that produced by classical Hsp ...
... Herein we show that the phenotype of Hsp90 knockdown cells using CRISPR technology is significantly different to that generated when cells are treated with classical inhibitors. We reveal that drugs acting via mechanisms unrelated to Hsp90 induce a similar phenotype to that produced by classical Hsp ...
DETERMINANTS: What`s in a Name?
... NIP1 protein from Rhynchosporium secalis (97, 160) all elicit a necrotic response that is macroscopically similar to that induced by most HSTs when introduced into the leaves of a genetically appropriate plant. Thus, absent the context of their primary role in disease, and based on the induced pheno ...
... NIP1 protein from Rhynchosporium secalis (97, 160) all elicit a necrotic response that is macroscopically similar to that induced by most HSTs when introduced into the leaves of a genetically appropriate plant. Thus, absent the context of their primary role in disease, and based on the induced pheno ...
Cell regulation by the Apc protein Apc as master regulator of epithelia
... The transcriptional changes induced by mutant Apc provide the background against which the other cellular functions of Apc become particularly important in the digestive tract epithelium. In addition to its function in the b-catenin destruction complex, Apc can also directly contribute to the regula ...
... The transcriptional changes induced by mutant Apc provide the background against which the other cellular functions of Apc become particularly important in the digestive tract epithelium. In addition to its function in the b-catenin destruction complex, Apc can also directly contribute to the regula ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... proteins of pumpkin, namely, 11S globulin and 2S albumin, are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessar ...
... proteins of pumpkin, namely, 11S globulin and 2S albumin, are not glycosylated. Recently, we isolated a cDNA of the third major storage protein of pumpkin, the 51-kD protein. The cDNA encodes the 100-kD precursor with no possible N-linked glycosylation site (data not shown). Thus, it is not necessar ...
this PDF file
... The unique ladderane lipids in anammox bacterial anammoxosome are fundamentally different from those of cytoplasmic membrane of bacteria [10,12], implying that the distinctive origins and biosynthetic pathways of this kind of lipids. Because the special chemical constitutes of ladderane lipids is mu ...
... The unique ladderane lipids in anammox bacterial anammoxosome are fundamentally different from those of cytoplasmic membrane of bacteria [10,12], implying that the distinctive origins and biosynthetic pathways of this kind of lipids. Because the special chemical constitutes of ladderane lipids is mu ...
In the prevailing view Conducitn is usually considered to act as a
... but, surprisingly, abolished puncta formation by Dvl2 (Fig. 3C). The M3 mutation impairs the head interaction surface of the axin DIX domain preventing its homopolymerisation, but leaves the tail interaction surface intact for interaction with Dvl2 (Fiedler et al., 2011). We propose that this result ...
... but, surprisingly, abolished puncta formation by Dvl2 (Fig. 3C). The M3 mutation impairs the head interaction surface of the axin DIX domain preventing its homopolymerisation, but leaves the tail interaction surface intact for interaction with Dvl2 (Fiedler et al., 2011). We propose that this result ...
Membrane immersion allows rhomboid proteases to achieve
... Site-2 proteases are metalloenzymes that release transcription factors from the membrane to regulate membrane biogenesis and stress responses in diverse organisms from bacterial pathogens to man (Rawson et al., 1997; Makinoshima and Glickman, 2005). Lastly, rhomboid proteases act as prime regulators ...
... Site-2 proteases are metalloenzymes that release transcription factors from the membrane to regulate membrane biogenesis and stress responses in diverse organisms from bacterial pathogens to man (Rawson et al., 1997; Makinoshima and Glickman, 2005). Lastly, rhomboid proteases act as prime regulators ...
VASCULAR TISSUE DIFFERENTIATION AND
... the plant hormones auxin and cytokinin are important for vascular differentiation (3, 77). A number of proteins and genes involved in different stages of tracheary element formation such as secondary wall thickening and cell death have been characterized using biochemical and subtractive hybridizati ...
... the plant hormones auxin and cytokinin are important for vascular differentiation (3, 77). A number of proteins and genes involved in different stages of tracheary element formation such as secondary wall thickening and cell death have been characterized using biochemical and subtractive hybridizati ...
Caspary T, Garc a-Garc a MJ, Eggenschwiler JR, Wyler MR, Huangfu D, Rakeman AS, Lee JD, Alcorn HL, Anderson KV. Curr Biol. 2002 Sep 17;12(18):1628-32. Mouse Dispatched homologue1 is required for long-range, but not juxtacrine, Hh signaling.
... loop in Disp1 contains six cysteines, including C829, which are conserved in all dispatched family members (Figure 5). The cysteine-to-phenylalanine change in the icb mutation is likely to disrupt a disulfide bond and cause destabilization of the loop or the protein. Because this mutation should dis ...
... loop in Disp1 contains six cysteines, including C829, which are conserved in all dispatched family members (Figure 5). The cysteine-to-phenylalanine change in the icb mutation is likely to disrupt a disulfide bond and cause destabilization of the loop or the protein. Because this mutation should dis ...
Translocation of Globin Fusion Proteins across the Endoplasmic
... preceding the signal sequence can be as large as that which follows it. While, in general, cell-free systems were found to faithfully reflect translocation events in vivo, our results suggest that a mechanism for clearance of signal peptides after cleavage is present in intact cells that is not reco ...
... preceding the signal sequence can be as large as that which follows it. While, in general, cell-free systems were found to faithfully reflect translocation events in vivo, our results suggest that a mechanism for clearance of signal peptides after cleavage is present in intact cells that is not reco ...
PDF - ScienceAsia
... acetate, hexane, or acetone) to re-precipitate to obtain a solid which then was filtered, dried, weighed, and subjected to further analysis. In parallel, solid-liquid extraction was investigated in comparison. A suspension of dry cells (0.50 g) in 10 ml of ethanol was shaken (130 rpm) at 30 °C for 1 ...
... acetate, hexane, or acetone) to re-precipitate to obtain a solid which then was filtered, dried, weighed, and subjected to further analysis. In parallel, solid-liquid extraction was investigated in comparison. A suspension of dry cells (0.50 g) in 10 ml of ethanol was shaken (130 rpm) at 30 °C for 1 ...
Photoreceptor Membrane Proteins, Phototransduction, and Retinal
... the capture of light and its conversion to an electrical signal. This membrane-rich organelle undergoes a continual renewal process in which newly synthesized membrane is added at the base of the outer segment, whereas packets of outer segment membrane are phagocytized at the distal end by the RPE c ...
... the capture of light and its conversion to an electrical signal. This membrane-rich organelle undergoes a continual renewal process in which newly synthesized membrane is added at the base of the outer segment, whereas packets of outer segment membrane are phagocytized at the distal end by the RPE c ...
Failsafe Mechanisms Coordinate Cell Division and the Initiation of
... the time it takes for chromosome replication to complete (40 minutes) [4, 5]. To solve this dilemma, bacteria employ multifork replication where initiations begin before the previous round of replication is ...
... the time it takes for chromosome replication to complete (40 minutes) [4, 5]. To solve this dilemma, bacteria employ multifork replication where initiations begin before the previous round of replication is ...
Here - Muscle Health Research Centre
... WHILE THE ABILITY for skeletal muscle to grow, adapt, and regenerate is well known, the numerous factors governing this ability are less well defined. Furthermore, satellite cells, the primary stem cell of skeletal muscle, are critical to these capacities of skeletal muscle and the factors governing ...
... WHILE THE ABILITY for skeletal muscle to grow, adapt, and regenerate is well known, the numerous factors governing this ability are less well defined. Furthermore, satellite cells, the primary stem cell of skeletal muscle, are critical to these capacities of skeletal muscle and the factors governing ...
Plant pathogen defense: Signalling, resistance and cell
... animals lack adaptive cells and rely on the innate immunity of each plant cell. There is however no doubt in the effectiveness of the plant immune system as most plants are healthy most of the time. The plant immune system consists of two main tiers of defense responses; the MAMP triggered immunity ...
... animals lack adaptive cells and rely on the innate immunity of each plant cell. There is however no doubt in the effectiveness of the plant immune system as most plants are healthy most of the time. The plant immune system consists of two main tiers of defense responses; the MAMP triggered immunity ...
HIV-1 Evolves into a Nonsyncytium-Inducing Virus upon Prolonged
... peak of infection. Unlike the LAI virus, the variant did not trigger the formation of syncytia. Our combined results suggest that the HIV-1 variant allows the infected host cell to survive longer, thus producing more viral progeny. The intricate virus-cell interaction results in a balance between op ...
... peak of infection. Unlike the LAI virus, the variant did not trigger the formation of syncytia. Our combined results suggest that the HIV-1 variant allows the infected host cell to survive longer, thus producing more viral progeny. The intricate virus-cell interaction results in a balance between op ...
Redox regulation of cysteine
... MnSOD (SOD2; localized to mitochondria) and CuZnSOD, with 2 types of CuZnSOD that are found either intracellularly (SOD1) or extracellularly (SOD3). These enzymes are responsible for the conversion of superoxide to H2O2, although this occurs slowly and spontaneously as well. Although superoxide is a ...
... MnSOD (SOD2; localized to mitochondria) and CuZnSOD, with 2 types of CuZnSOD that are found either intracellularly (SOD1) or extracellularly (SOD3). These enzymes are responsible for the conversion of superoxide to H2O2, although this occurs slowly and spontaneously as well. Although superoxide is a ...
TBX5 is required for embryonic cardiac cell cycle - UNC
... abnormalities include atrial and ventricular septal defects, as well as conductivity defects and aberrant chamber formation (Basson et al., 1999; Benson et al., 1996; Newbury-Ecob et al., 1996). A role for Tbx5 in HOS is supported by the observation that mice heterozygous for mutations in Tbx5 displ ...
... abnormalities include atrial and ventricular septal defects, as well as conductivity defects and aberrant chamber formation (Basson et al., 1999; Benson et al., 1996; Newbury-Ecob et al., 1996). A role for Tbx5 in HOS is supported by the observation that mice heterozygous for mutations in Tbx5 displ ...
Characterization of the Enzymatic Component of the ADP
... ADP-ribosylating toxin. The toxin consists of the binding component CDTb, which mediates receptor binding and cellular uptake, and the enzyme component CDTa. Here we studied the enzyme component (CDTa) of the toxin using the binding component of Clostridium perfringens iota toxin (Ib), which is inte ...
... ADP-ribosylating toxin. The toxin consists of the binding component CDTb, which mediates receptor binding and cellular uptake, and the enzyme component CDTa. Here we studied the enzyme component (CDTa) of the toxin using the binding component of Clostridium perfringens iota toxin (Ib), which is inte ...
Pre-operative HBO treatment led to enhanced expression of
... nuclei (35) suggests that the nucleus may have some capacity of this kind as well. Keeping in mind that GM3 precursors ceramide and sphingosine accumulated in nuclei may induce the apoptosis of hepatocytes in vivo, enhanced GM3 expression around the nuclei could have important implications. Namely, ...
... nuclei (35) suggests that the nucleus may have some capacity of this kind as well. Keeping in mind that GM3 precursors ceramide and sphingosine accumulated in nuclei may induce the apoptosis of hepatocytes in vivo, enhanced GM3 expression around the nuclei could have important implications. Namely, ...
phosphatases and differentiation of the golgi apparatus
... central region subjacent to the apex proper is composed of cap initial cells which divide to give rise to additional cap cells. In this region there are none of the large, Golgi apparatus secretory vesicles (Fig. 2 A). Towards the outer cap there is a progressive build-up of secretory-vesicle produc ...
... central region subjacent to the apex proper is composed of cap initial cells which divide to give rise to additional cap cells. In this region there are none of the large, Golgi apparatus secretory vesicles (Fig. 2 A). Towards the outer cap there is a progressive build-up of secretory-vesicle produc ...
FasL gene knock-down therapy enhances the
... effect on the in vitro growth characteristics of the cloned cell lines (data not shown). The effects of FasL knock-down on Jurkat cell death was examined in the glioma:Jurkat cell cocultures. The magnitude of Jurkat cell protection in cocultures using FasL knockdown cells was essentially identical t ...
... effect on the in vitro growth characteristics of the cloned cell lines (data not shown). The effects of FasL knock-down on Jurkat cell death was examined in the glioma:Jurkat cell cocultures. The magnitude of Jurkat cell protection in cocultures using FasL knockdown cells was essentially identical t ...
The Plant Actin Cytoskeleton Responds to Signals from Microbe
... actin cytoskeleton in Arabidopsis epidermal cells represent a surveillance mechanism to external threats [1]; however, it is still unclear whether there are direct links between actin and PTI- or effector-mediated signal transduction cascades or which actinbinding proteins are involved. Significantl ...
... actin cytoskeleton in Arabidopsis epidermal cells represent a surveillance mechanism to external threats [1]; however, it is still unclear whether there are direct links between actin and PTI- or effector-mediated signal transduction cascades or which actinbinding proteins are involved. Significantl ...
Starvation Induces Vacuolar Targeting and Degradation of the
... amino acid permeases consists of mainly high affinity transporters that are specific for single amino acids or a small set of structurally related amino acids. These permeases, including the histidine permease, HIP1 (Tanaka and Fink, 1985), and the tryptophan permease, TAT2 (Schmidt et al., 1994), i ...
... amino acid permeases consists of mainly high affinity transporters that are specific for single amino acids or a small set of structurally related amino acids. These permeases, including the histidine permease, HIP1 (Tanaka and Fink, 1985), and the tryptophan permease, TAT2 (Schmidt et al., 1994), i ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.