Recent developments in the cell and molecular biology of root hairs
... second in transvacuolar strands. These authors also showed that the cessation of streaming caused by the toxin mycalolide B cannot be reversed (mycalolide B causes irreversible disorganisation of actin filaments) in comparison with the effects of cytochalasin B which are reversible. There are many r ...
... second in transvacuolar strands. These authors also showed that the cessation of streaming caused by the toxin mycalolide B cannot be reversed (mycalolide B causes irreversible disorganisation of actin filaments) in comparison with the effects of cytochalasin B which are reversible. There are many r ...
Translocation of proteins across archaeal cytoplasmic membranes
... life under extreme conditions, but have also provided novel insights into bacterial and eukaryotic cellular function. One such cellular process is that of protein translocation. 1.2. Protein translocation The process of protein translocation into or across hydrophobic membranes is essential to all l ...
... life under extreme conditions, but have also provided novel insights into bacterial and eukaryotic cellular function. One such cellular process is that of protein translocation. 1.2. Protein translocation The process of protein translocation into or across hydrophobic membranes is essential to all l ...
AHEART December 46/6 - AJP
... were then examined to determine cell morphology (rod, round, or square) and permeability (blue vs. not blue), and the results were averaged for each group (4). More than 300 cells were examined in each sample. Cells that were not able to exclude trypan blue were considered to have membrane failure a ...
... were then examined to determine cell morphology (rod, round, or square) and permeability (blue vs. not blue), and the results were averaged for each group (4). More than 300 cells were examined in each sample. Cells that were not able to exclude trypan blue were considered to have membrane failure a ...
A Pd8 Tetrafacial Molecular Barrel as Carrier for Water Insoluble
... diagnostics as well as biological studies in order to track dynamics and functions of various cellular processes. The foremost important factors associated with a cell imaging fluorophore are membrane permeability, cell viability, solubility, and stability.15 Various probes have been developed for th ...
... diagnostics as well as biological studies in order to track dynamics and functions of various cellular processes. The foremost important factors associated with a cell imaging fluorophore are membrane permeability, cell viability, solubility, and stability.15 Various probes have been developed for th ...
PDZ proteins retain and regulate membrane transporters in
... (ZO)-1 (30)—are crucial components in both processes (for review, see Refs. 63, 64). In this review, we focus on the role of PDZ proteins in the polarized expression and function of membrane transporters (e.g., ion channels, exchangers, cotransporters) in epithelial cells, mainly of renal origin. In ...
... (ZO)-1 (30)—are crucial components in both processes (for review, see Refs. 63, 64). In this review, we focus on the role of PDZ proteins in the polarized expression and function of membrane transporters (e.g., ion channels, exchangers, cotransporters) in epithelial cells, mainly of renal origin. In ...
Phalloidin
... Please visit www.biotium.com to view our full selection of CF™ dye and R-PE conjugates, including labeled primary and secondary antibodies, streptavidin, Annexin V, a-bungarotoxin, and Mix-n-Stain antibody labeling kits. Biotium also offers a variety of apoptosis and cell viability assays for flow c ...
... Please visit www.biotium.com to view our full selection of CF™ dye and R-PE conjugates, including labeled primary and secondary antibodies, streptavidin, Annexin V, a-bungarotoxin, and Mix-n-Stain antibody labeling kits. Biotium also offers a variety of apoptosis and cell viability assays for flow c ...
Direct Evidence of Active and Rapid Nuclear
... plants (Pennell and Lamb, 1997), also exhibits morphological features closer to necrosis (Fukuda, 1998). Extensive studies about PCD during TE differentiation have been performed using the zinnia (Zinnia elegans) culture system established by Fukuda and Komamine (1980a). In the zinnia cell cultures, ...
... plants (Pennell and Lamb, 1997), also exhibits morphological features closer to necrosis (Fukuda, 1998). Extensive studies about PCD during TE differentiation have been performed using the zinnia (Zinnia elegans) culture system established by Fukuda and Komamine (1980a). In the zinnia cell cultures, ...
Distinct Protease Requirements for Antigen Presentation In Vitro and
... exposed to lysosomal fractions purified from EBV-transformed human B cells (12, 13). Mutagenesis of the three principle AEP cleavage sites in TTCF or suppression of AEP activity inhibited TTCF presentation to T cells in vitro (12–15). AEP is also one of several enzymes able to initiate the processin ...
... exposed to lysosomal fractions purified from EBV-transformed human B cells (12, 13). Mutagenesis of the three principle AEP cleavage sites in TTCF or suppression of AEP activity inhibited TTCF presentation to T cells in vitro (12–15). AEP is also one of several enzymes able to initiate the processin ...
The Sec61p Complex Mediates the Integration of a Membrane
... the hydrophobicity of the TM domain and the length of the polypeptide segment tethering it to the ribosome. Our results suggest a unifying, lipid-partitioning model which can explain the general behavior of hydrophobic topogenic sequences. Introduction Most eukaryotic membrane proteins are cotransla ...
... the hydrophobicity of the TM domain and the length of the polypeptide segment tethering it to the ribosome. Our results suggest a unifying, lipid-partitioning model which can explain the general behavior of hydrophobic topogenic sequences. Introduction Most eukaryotic membrane proteins are cotransla ...
Intraflagellar transport molecules in ciliary and nonciliary cells of the
... raised to murine IFT proteins (Pazour et al., 2002; Baker et al., 2003; Follit et al., 2006). These experiments verified the expression of all five IFT proteins in the murine retina (Fig. S1, A and B). The antibodies to four IFT proteins each recognized a single band of expected size in retinal prot ...
... raised to murine IFT proteins (Pazour et al., 2002; Baker et al., 2003; Follit et al., 2006). These experiments verified the expression of all five IFT proteins in the murine retina (Fig. S1, A and B). The antibodies to four IFT proteins each recognized a single band of expected size in retinal prot ...
Characterization of the Cytosolic Proteins Involved in the Amoeboid
... cytoskeleton and can be broken down into three essential steps (Figure 1): protrusion of the leading edge, adhesion to the substrate, and retraction of the cell body (Mitchison and Cramer, 1996). Protrusion involves localized actin polymerization at the leading edge membrane, which ultimately leads ...
... cytoskeleton and can be broken down into three essential steps (Figure 1): protrusion of the leading edge, adhesion to the substrate, and retraction of the cell body (Mitchison and Cramer, 1996). Protrusion involves localized actin polymerization at the leading edge membrane, which ultimately leads ...
Physical properties of lipid bilayer membranes: relevance to
... Because both oxygen and nitric oxide (NO) are paramagnetic and have similar physical properties, a similar approach can be used to study NO transport within and across the lipid bilayer membrane [42]. Data obtained by us [43, 44] give a solid base for the conclusion that fluid-phase membranes are no ...
... Because both oxygen and nitric oxide (NO) are paramagnetic and have similar physical properties, a similar approach can be used to study NO transport within and across the lipid bilayer membrane [42]. Data obtained by us [43, 44] give a solid base for the conclusion that fluid-phase membranes are no ...
Characterization of the ftsYEX operon of Escherichia coli
... the unicellular organism. Composed of phospholipids, these bilayers are boundaries between "in" and "out", presenting a selective barrier to the potential harm of an external environment while remaining permeable to the elements essential for life also present in that environment. New membranes must ...
... the unicellular organism. Composed of phospholipids, these bilayers are boundaries between "in" and "out", presenting a selective barrier to the potential harm of an external environment while remaining permeable to the elements essential for life also present in that environment. New membranes must ...
Genetic Analysis of Female Gametophyte Development and Function
... Female Gametophyte Mutations Fall into Two Classes The known female gametophyte mutations are listed in Table 2. Many (11/25) of these mutations do not affect the male gametophyte and thus fall into the female gametophyte–specific class ( Table 2). Female gametophyte–specific mutations are potential ...
... Female Gametophyte Mutations Fall into Two Classes The known female gametophyte mutations are listed in Table 2. Many (11/25) of these mutations do not affect the male gametophyte and thus fall into the female gametophyte–specific class ( Table 2). Female gametophyte–specific mutations are potential ...
The transplantation of nuclei from single cultured cells into
... The most normal development promoted by cultured-cell nuclei. The most normal nuclear-transplant embryos are of interest, partly because they place a lower limit on the extent to which it is possible to transplant cultured-cell nuclei without damage, and partly because they test the presence and exp ...
... The most normal development promoted by cultured-cell nuclei. The most normal nuclear-transplant embryos are of interest, partly because they place a lower limit on the extent to which it is possible to transplant cultured-cell nuclei without damage, and partly because they test the presence and exp ...
Title Nanofiltration and reverse osmosis surface topographical
... publication in Journal of Membrane Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for ...
... publication in Journal of Membrane Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for ...
Anti-bacteria and In Vivo Tumor Treatment by Reactive Oxygen
... Fluorescence microscope of the E. Coli The further investigated the anti-bacterial activity of 6-nm MNPs in the presence of H2O2. The dead E. coli was investigated with fluorescence microscope (Leica DMI 6000B). During the experiment, E. coli was incubated to 6 × 108 CFU/mL, and then MNPs (20 g/mL) ...
... Fluorescence microscope of the E. Coli The further investigated the anti-bacterial activity of 6-nm MNPs in the presence of H2O2. The dead E. coli was investigated with fluorescence microscope (Leica DMI 6000B). During the experiment, E. coli was incubated to 6 × 108 CFU/mL, and then MNPs (20 g/mL) ...
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... The most normal development promoted by cultured-cell nuclei. The most normal nuclear-transplant embryos are of interest, partly because they place a lower limit on the extent to which it is possible to transplant cultured-cell nuclei without damage, and partly because they test the presence and exp ...
... The most normal development promoted by cultured-cell nuclei. The most normal nuclear-transplant embryos are of interest, partly because they place a lower limit on the extent to which it is possible to transplant cultured-cell nuclei without damage, and partly because they test the presence and exp ...
Fungi
... and terrestrial habitats – Are defined by the production of sexual spores in saclike asci, which are usually contained in fruiting bodies called ascocarps – Have asexual reproduction by producing enormous numbers of asexual spores called ...
... and terrestrial habitats – Are defined by the production of sexual spores in saclike asci, which are usually contained in fruiting bodies called ascocarps – Have asexual reproduction by producing enormous numbers of asexual spores called ...
Unraveling the complex network of cuticular structure and function
... insects [4,10]. Potentially, the cuticle might also be involved in the generation and distribution of signals in development and in plant pathogen–interactions. Aspects that have been recently reviewed will not been discussed in detail here [1,14,42,43]. The role of the cuticle as a barrier to water ...
... insects [4,10]. Potentially, the cuticle might also be involved in the generation and distribution of signals in development and in plant pathogen–interactions. Aspects that have been recently reviewed will not been discussed in detail here [1,14,42,43]. The role of the cuticle as a barrier to water ...
Numerical simulation of morphogenetic movements in
... therefore a comparison between the two is proposed together with the advantages and the limitations of our study. Finally, we provide some simple examples that validate our model and support the exploited method. The third chapter can be divided into two parts. In the first one, by the parametrical ...
... therefore a comparison between the two is proposed together with the advantages and the limitations of our study. Finally, we provide some simple examples that validate our model and support the exploited method. The third chapter can be divided into two parts. In the first one, by the parametrical ...
Western blot Fast purification Comparative performance
... series, a magazine dedicated to discussing new and emerging trends relevant to the field of life science for our Australian and New Zealand customers. The focus of this edition is advances in technology in Protein Research. We have included articles covering both upstream and downstream processes, a ...
... series, a magazine dedicated to discussing new and emerging trends relevant to the field of life science for our Australian and New Zealand customers. The focus of this edition is advances in technology in Protein Research. We have included articles covering both upstream and downstream processes, a ...
Light-Dependent Intracellular Positioning of Mitochondria in
... detachments. Their movement ranged from small oscillations of <1 µm to a large-scale displacement of >10 µm s−1. Typical examples obtained at the start (Fig. 5) and after 4 h (Fig. 6) of illumination with wBL are presented. From such recordings, the movement of each mitochondrion was traced (Fig. 7) ...
... detachments. Their movement ranged from small oscillations of <1 µm to a large-scale displacement of >10 µm s−1. Typical examples obtained at the start (Fig. 5) and after 4 h (Fig. 6) of illumination with wBL are presented. From such recordings, the movement of each mitochondrion was traced (Fig. 7) ...
A Critical Role for Egr-1 Characterization of CD44 Induction by IL-1:
... (8). Furthermore, three studies have shown that an anti-CD44 Ab, IM7, has antiinflammatory effects in several in vivo models of inflammation (15–17). Finally, binding of low m.w. fragments of hyaluronic acid (HA) to alveolar macrophages via CD44 elicits the expression of a number of proinflammatory ...
... (8). Furthermore, three studies have shown that an anti-CD44 Ab, IM7, has antiinflammatory effects in several in vivo models of inflammation (15–17). Finally, binding of low m.w. fragments of hyaluronic acid (HA) to alveolar macrophages via CD44 elicits the expression of a number of proinflammatory ...
CASK (LIN2) interacts with Cx43 in wounded skin and their
... Cx43, the most ubiquitous connexin, oligomerizes into a hexamer or ‘‘connexon’’ in the trans-Golgi network (TGN) (Musil and Goodenough, 1993) and, after transport to the plasma membrane and association with other connexons, can form a gap junction. Zona Occudens-1 (ZO-1), a member of the membraneass ...
... Cx43, the most ubiquitous connexin, oligomerizes into a hexamer or ‘‘connexon’’ in the trans-Golgi network (TGN) (Musil and Goodenough, 1993) and, after transport to the plasma membrane and association with other connexons, can form a gap junction. Zona Occudens-1 (ZO-1), a member of the membraneass ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.