51:64-72 - Boston College
... electroporation protocol to specifically study the wiring of Xenopus embryonic brains between stages 21–40. Depending upon the region of interest, commonly either brain or eye, embryos are positioned in electroporation chambers tailored to fit their developmental stage. For eye-targeted delivery, a so ...
... electroporation protocol to specifically study the wiring of Xenopus embryonic brains between stages 21–40. Depending upon the region of interest, commonly either brain or eye, embryos are positioned in electroporation chambers tailored to fit their developmental stage. For eye-targeted delivery, a so ...
Monitoring Growth of Beer Brewing Strains of
... each overnight stock culture was diluted to 7.5 mL with fresh 1X YPD media. The diluted cells were then plated as needed into Corning 3598 flat-bottomed clear plates. Measurements were made every 2 minutes with continuous orbital shaking mode employed. Shaking speed was set to slow and frequency set ...
... each overnight stock culture was diluted to 7.5 mL with fresh 1X YPD media. The diluted cells were then plated as needed into Corning 3598 flat-bottomed clear plates. Measurements were made every 2 minutes with continuous orbital shaking mode employed. Shaking speed was set to slow and frequency set ...
The location ofthe third cleavage plane of Xenopus embryos
... inversion and 020 immersion increased AVCR (like Jlg-treatment) and cold shock reduced A VCR (like hypergravity treatment), the primary cause of AVCR changes was postulated to be an alteration in the distribution of dense cytoplasmic components such as yolk platelets and the rearrangement of microtu ...
... inversion and 020 immersion increased AVCR (like Jlg-treatment) and cold shock reduced A VCR (like hypergravity treatment), the primary cause of AVCR changes was postulated to be an alteration in the distribution of dense cytoplasmic components such as yolk platelets and the rearrangement of microtu ...
Slits affect the timely migration of neural crest cells via robo receptor
... Slit1 (A), Slit2 (B), Slit3 (C), Robo1 (D), and Robo2 (E) anti-sense probes. HH12–13 chicken embryos showed expression of Slit ligands in dorsal neural tube (arrows in H–K sections for Slit1 and Slit2). Robo1 receptor is expressed in the medial somites (red arrow in D) but also in the neural tube at ...
... Slit1 (A), Slit2 (B), Slit3 (C), Robo1 (D), and Robo2 (E) anti-sense probes. HH12–13 chicken embryos showed expression of Slit ligands in dorsal neural tube (arrows in H–K sections for Slit1 and Slit2). Robo1 receptor is expressed in the medial somites (red arrow in D) but also in the neural tube at ...
Brief Report - The Journal of Cell Biology
... (Kirkegaard & Perry Laboratories, Inc.) at 1:40 in 0.5% BSA/PBS for 30 min at 378C, slides were washed three times with PBS and mounted. For cell invasion assays, z105 HepG2 cells were seeded in eight chamber slides and grown to semiconfluency. Sporozoites (z15,000/well) were added, incubated 2 h at ...
... (Kirkegaard & Perry Laboratories, Inc.) at 1:40 in 0.5% BSA/PBS for 30 min at 378C, slides were washed three times with PBS and mounted. For cell invasion assays, z105 HepG2 cells were seeded in eight chamber slides and grown to semiconfluency. Sporozoites (z15,000/well) were added, incubated 2 h at ...
Effect of ABA signalling in primary cell wall Minor Thesis Report
... a heterogeneous group of polysaccharides formed by different biosynthetic routes from cellulose. Most of hemicelluloses are functioning like cellulose, to support the material within the plant primary cell wall which together forming complex and dynamic structure controlling cell shape and plant mor ...
... a heterogeneous group of polysaccharides formed by different biosynthetic routes from cellulose. Most of hemicelluloses are functioning like cellulose, to support the material within the plant primary cell wall which together forming complex and dynamic structure controlling cell shape and plant mor ...
Conditioning of Roots with Hypoxia Increases
... Aluminum (Al) is prevalent in soils, but Al toxicity is manifested only under acid conditions. It causes severe damages to the root system. Short-term waterlogging stress can occur simultaneously with Al toxicity in areas with high rainfall or an inappropriate irrigation pattern. Barley (Hordeum vul ...
... Aluminum (Al) is prevalent in soils, but Al toxicity is manifested only under acid conditions. It causes severe damages to the root system. Short-term waterlogging stress can occur simultaneously with Al toxicity in areas with high rainfall or an inappropriate irrigation pattern. Barley (Hordeum vul ...
In tobacco leaf epidermal cells, the integrity of protein export from
... Trafficking of secretory proteins between the endoplasmic reticulum (ER) and the Golgi apparatus depends on coat protein complexes I (COPI) and II (COPII) machineries. To date, full characterization of the distribution and dynamics of these machineries in plant cells remains elusive. Furthermore, ex ...
... Trafficking of secretory proteins between the endoplasmic reticulum (ER) and the Golgi apparatus depends on coat protein complexes I (COPI) and II (COPII) machineries. To date, full characterization of the distribution and dynamics of these machineries in plant cells remains elusive. Furthermore, ex ...
OPTIMISATION OF CHO CELL GROWTH AND
... linoleic acid, was able to restore cell growth in low BSA (1 mg/ml) cultures. However, JFN-y production was significantly reduced and the extent of IFN-y glycosylation also changed. These effects were related to: (1) BSA concentration, (2) BSA type, and ultimately, (3) lipid composition of the cultu ...
... linoleic acid, was able to restore cell growth in low BSA (1 mg/ml) cultures. However, JFN-y production was significantly reduced and the extent of IFN-y glycosylation also changed. These effects were related to: (1) BSA concentration, (2) BSA type, and ultimately, (3) lipid composition of the cultu ...
In tobacco leaf epidermal cells, the integrity of protein export from
... Trafficking of secretory proteins between the endoplasmic reticulum (ER) and the Golgi apparatus depends on coat protein complexes I (COPI) and II (COPII) machineries. To date, full characterization of the distribution and dynamics of these machineries in plant cells remains elusive. Furthermore, ex ...
... Trafficking of secretory proteins between the endoplasmic reticulum (ER) and the Golgi apparatus depends on coat protein complexes I (COPI) and II (COPII) machineries. To date, full characterization of the distribution and dynamics of these machineries in plant cells remains elusive. Furthermore, ex ...
CHAPTER 5 THE MULTIPLIER 5.1 INTRODUCTION
... together M partial products. Each partial product is generated by multiplying the multiplicand with a bit of the multiplier – which, essentially, is an AND operation – and by shifting the result in the basis of the multiplier bit’s position. Similar to the familiar long hand decimal multiplication, ...
... together M partial products. Each partial product is generated by multiplying the multiplicand with a bit of the multiplier – which, essentially, is an AND operation – and by shifting the result in the basis of the multiplier bit’s position. Similar to the familiar long hand decimal multiplication, ...
The GDP-bound form of Arf6 is located at the plasma membrane
... implicated in endocytosis and actin rearrangements at the cell periphery (Chavrier and Goud, 1999) but its precise function remains to be discovered. Two mutants of Arf6, Q67L and T27N, are considered to mimic the GTP- and GDP-bound forms, respectively, and have been used extensively to apprehend th ...
... implicated in endocytosis and actin rearrangements at the cell periphery (Chavrier and Goud, 1999) but its precise function remains to be discovered. Two mutants of Arf6, Q67L and T27N, are considered to mimic the GTP- and GDP-bound forms, respectively, and have been used extensively to apprehend th ...
hematopoietic stem cells: to be or notch to be
... generate a network of biochemical interactions and are ultimately translated into precise instructions at the nuclear level. The Notch pathway has been evolutionary preserved not only biochemically but also functionally, being involved in establishing cell diversification from equipotent adjacent ce ...
... generate a network of biochemical interactions and are ultimately translated into precise instructions at the nuclear level. The Notch pathway has been evolutionary preserved not only biochemically but also functionally, being involved in establishing cell diversification from equipotent adjacent ce ...
- Wiley Online Library
... observed transmit EIX-induced signaling, as analysis of the movies with and without EIX treatment leads to the observation that only a subpopulation of endosomes are directional in response to EIX, while many endosomes remain random in movement, and yet additional endosomes have very little motility ...
... observed transmit EIX-induced signaling, as analysis of the movies with and without EIX treatment leads to the observation that only a subpopulation of endosomes are directional in response to EIX, while many endosomes remain random in movement, and yet additional endosomes have very little motility ...
Keystone Standards
... BIO.A.4. 1. Identify and describe the cell structures involved in transport of materials into, out of, and throughout a cell. BIO.A.4. 1.1. Describe how the structure of the plasma membrane allows it to function as a regulatory structure and/or protective barrier for a cell. BIO.A.4. 1.2. Compare th ...
... BIO.A.4. 1. Identify and describe the cell structures involved in transport of materials into, out of, and throughout a cell. BIO.A.4. 1.1. Describe how the structure of the plasma membrane allows it to function as a regulatory structure and/or protective barrier for a cell. BIO.A.4. 1.2. Compare th ...
Arabinogalactan proteins are involved in root hair development in
... recognizing specific epitopes associated with the carbohydrate moieties have been used (Nguema-Ona et al., 2012). The role of AGPs during root development and morphogenesis was shown using the active form of Yariv reagent. Exposure of the Arabidopsis thaliana root to βGlcY suppresses the elongation ...
... recognizing specific epitopes associated with the carbohydrate moieties have been used (Nguema-Ona et al., 2012). The role of AGPs during root development and morphogenesis was shown using the active form of Yariv reagent. Exposure of the Arabidopsis thaliana root to βGlcY suppresses the elongation ...
Transcriptionally repressed germ cells lack a
... One possibility is that newly transcribed mRNAs are rapidly ...
... One possibility is that newly transcribed mRNAs are rapidly ...
M6PRs are found in a subset of PC12 cell ISGs
... The M6PRs and furin are found in TGN membranes, endosomes and the plasma membrane, and appear to cycle through these compartments (for review see Le Borgne and Hoflack, 1998 and Molloy et al., 1999). The cytoplasmic domains of M6PR and furin interact with either AP-1 in the TGN, or AP-2 at the plasm ...
... The M6PRs and furin are found in TGN membranes, endosomes and the plasma membrane, and appear to cycle through these compartments (for review see Le Borgne and Hoflack, 1998 and Molloy et al., 1999). The cytoplasmic domains of M6PR and furin interact with either AP-1 in the TGN, or AP-2 at the plasm ...
Cytokinin–auxin crosstalk
... most-studied auxin transporters are the PIN genes, which form a small family of eight members, most of which appear to facilitate cellular auxin efflux [28] (Figure 1). Members of the PIN protein family are homologous and functionally redundant, as indicated by the increasingly severe phenotypes of ...
... most-studied auxin transporters are the PIN genes, which form a small family of eight members, most of which appear to facilitate cellular auxin efflux [28] (Figure 1). Members of the PIN protein family are homologous and functionally redundant, as indicated by the increasingly severe phenotypes of ...
Transcriptionally repressed germ cells lack a subpopulation of
... One possibility is that newly transcribed mRNAs are rapidly ...
... One possibility is that newly transcribed mRNAs are rapidly ...
Skeletal Muscle
... in the unit on the physiology of muscle contraction. The cytosol is the cytoplasm of the muscle cell. The sarcoplasmic reticulum is the endoplasmic reticulum of the muscle cell. There are sac-like regions of the sarcoplasmic reticulum known as terminal cisternae. The terminal cisternae act as calciu ...
... in the unit on the physiology of muscle contraction. The cytosol is the cytoplasm of the muscle cell. The sarcoplasmic reticulum is the endoplasmic reticulum of the muscle cell. There are sac-like regions of the sarcoplasmic reticulum known as terminal cisternae. The terminal cisternae act as calciu ...
Notochord vacuoles are lysosome-related organelles that function in
... the embryo before bone formation. This axial support is crucial for locomotion in embryos that develop externally, such as fish and amphibians, to evade predation. The pressure exerted from the inflating notochord vacuoles on the constricting perinotochordal basement membrane gives this structure it ...
... the embryo before bone formation. This axial support is crucial for locomotion in embryos that develop externally, such as fish and amphibians, to evade predation. The pressure exerted from the inflating notochord vacuoles on the constricting perinotochordal basement membrane gives this structure it ...
Notochord vacuoles are lysosome-related organelles that function in
... the embryo before bone formation. This axial support is crucial for locomotion in embryos that develop externally, such as fish and amphibians, to evade predation. The pressure exerted from the inflating notochord vacuoles on the constricting perinotochordal basement membrane gives this structure it ...
... the embryo before bone formation. This axial support is crucial for locomotion in embryos that develop externally, such as fish and amphibians, to evade predation. The pressure exerted from the inflating notochord vacuoles on the constricting perinotochordal basement membrane gives this structure it ...
O-GlcNAc transferase inhibitors: current tools and
... Biochemical Society Transactions (2016) Volume 44, part 1 ...
... Biochemical Society Transactions (2016) Volume 44, part 1 ...
Structure and mechanism of ATP-dependent phospholipid transporters
... Biological membranes are the basis for highly defined and separated functional units. The cell membrane, or plasma membrane, defines the external boundary of every cell separating the cytoplasm from the surrounding environment. Eukaryotic cells contain in addition numerous subcellular membranes that d ...
... Biological membranes are the basis for highly defined and separated functional units. The cell membrane, or plasma membrane, defines the external boundary of every cell separating the cytoplasm from the surrounding environment. Eukaryotic cells contain in addition numerous subcellular membranes that d ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.