Actin-based motility of endosomes is linked to the polar tip growth of
... In both yeast and mammals, endosomes recruit FYVE-domain proteins due to high levels of PI(3)P when the FYVE sequence acts as a PI(3)P-binding module (Gillooly et al., 2000; Jensen et al., 2001). This is mediated via specific recruitment of PI3Ks, supporting local production of PI(3)P to these membra ...
... In both yeast and mammals, endosomes recruit FYVE-domain proteins due to high levels of PI(3)P when the FYVE sequence acts as a PI(3)P-binding module (Gillooly et al., 2000; Jensen et al., 2001). This is mediated via specific recruitment of PI3Ks, supporting local production of PI(3)P to these membra ...
The Expression of MHC Class II Genes in Macrophages Is Cell
... could also affect the translational apparatus and regulate in a CCD way the translation of several genes (7, 8). Therefore, there are genes induced in a CCD fashion that are not involved in cell cycle progression. This is the case of genes that control some cellular activities. For example, it has b ...
... could also affect the translational apparatus and regulate in a CCD way the translation of several genes (7, 8). Therefore, there are genes induced in a CCD fashion that are not involved in cell cycle progression. This is the case of genes that control some cellular activities. For example, it has b ...
The ~53 Proto-Oncogene Can Act as a Suppressor of Transformation
... of the cellular retinoblastoma (Rb) gene (DeCaprio et al., 1988; Whyte et al., 1988). Deletion or inactivation of the Rb gene predisposes individuals to the development of human malignant retinoblastoma (Friend et al., 1986; Lee et al., 1987), and abnormalities in the Rb locus have recently been det ...
... of the cellular retinoblastoma (Rb) gene (DeCaprio et al., 1988; Whyte et al., 1988). Deletion or inactivation of the Rb gene predisposes individuals to the development of human malignant retinoblastoma (Friend et al., 1986; Lee et al., 1987), and abnormalities in the Rb locus have recently been det ...
PDF - Eleanor Maine Research Lab
... The C. elegans germline provides an excellent model for analyzing the regulation of stem cell activity and the decision to differentiate and undergo meiotic development. The distal end of the adult hermaphrodite germline contains the proliferative zone, which includes a population of mitotically cyc ...
... The C. elegans germline provides an excellent model for analyzing the regulation of stem cell activity and the decision to differentiate and undergo meiotic development. The distal end of the adult hermaphrodite germline contains the proliferative zone, which includes a population of mitotically cyc ...
Labeling Proteins with Small Molecules by Site
... Western blotting showed that only the target proteins in the cell lysate were labeled with biotin, suggesting the high specificity of the labeling reactions.9 Chasing the reaction mixture with 3H-acetyl CoA showed that the labeling reaction is more than 95% complete after 30 min, suggesting the high ...
... Western blotting showed that only the target proteins in the cell lysate were labeled with biotin, suggesting the high specificity of the labeling reactions.9 Chasing the reaction mixture with 3H-acetyl CoA showed that the labeling reaction is more than 95% complete after 30 min, suggesting the high ...
Growth Control and Cell Wall Signaling in Plants
... as potential cross-linking sites (105). Arabidopsis has 20 highly similar extensin genes (30). Mutant analysis shows an essential role for certain extensins in primary cell wall assembly. For instance, AtEXT3 is present in cell plates and mature walls, and loss-of-function mutants for the correspond ...
... as potential cross-linking sites (105). Arabidopsis has 20 highly similar extensin genes (30). Mutant analysis shows an essential role for certain extensins in primary cell wall assembly. For instance, AtEXT3 is present in cell plates and mature walls, and loss-of-function mutants for the correspond ...
Overexpression of a truncated cyclin B gene arrests Dictyostelium
... part of the G2 phase also have a preference to become prestalk cells (Maeda et al., 1989). A variety of low molecular mass factors have also been shown to influence the formation of both stalk and spore cells, and these molecules have been proposed as morphogens responsible for generating and mainta ...
... part of the G2 phase also have a preference to become prestalk cells (Maeda et al., 1989). A variety of low molecular mass factors have also been shown to influence the formation of both stalk and spore cells, and these molecules have been proposed as morphogens responsible for generating and mainta ...
viability. Bacterial plasmolysis as a physical
... reinoculating flow cells containing heat-killed cells with log-phase S. enteritidis cells. Similarly, biofilms containing a mixture of viable and formalin-killed cells were prepared by reinoculating flow cells containing formalin-killed cells with log-phase S. enteritidis cells while the nutrient fl ...
... reinoculating flow cells containing heat-killed cells with log-phase S. enteritidis cells. Similarly, biofilms containing a mixture of viable and formalin-killed cells were prepared by reinoculating flow cells containing formalin-killed cells with log-phase S. enteritidis cells while the nutrient fl ...
full lab details and projects
... Our long-term goal is to understand the mechanisms regulating cilia assembly and why defects in these processes cause photoreceptor degeneration. Vertebrate photoreceptor outer segments form from the connecting cilium, which is anchored by a basal body at the apical inner segment. The connecting cil ...
... Our long-term goal is to understand the mechanisms regulating cilia assembly and why defects in these processes cause photoreceptor degeneration. Vertebrate photoreceptor outer segments form from the connecting cilium, which is anchored by a basal body at the apical inner segment. The connecting cil ...
STAINING
... method uses heat as part of the staining process, whereas the K method is a ―cold‖ stain. In both protocols, the bacterial smear may be prepared in a drop of serum to help the ―slippery‖ The waxy wall .acidfast cells adhere to the slide. The two methods provide comparable results of acid-fast cells ...
... method uses heat as part of the staining process, whereas the K method is a ―cold‖ stain. In both protocols, the bacterial smear may be prepared in a drop of serum to help the ―slippery‖ The waxy wall .acidfast cells adhere to the slide. The two methods provide comparable results of acid-fast cells ...
Tracheary Element Differentiation
... Annu. Rev. Plant Biol. 2007.58:407-433. Downloaded from arjournals.annualreviews.org by Swedish University of Agricultural Sciences on 09/03/07. For personal use only. ...
... Annu. Rev. Plant Biol. 2007.58:407-433. Downloaded from arjournals.annualreviews.org by Swedish University of Agricultural Sciences on 09/03/07. For personal use only. ...
Chap2_110718_textbook
... Meiosis, like mitosis, is also a necessary part of cell division. However, in meiosis not only do sister chromatids separate from each other, homologous chromosomes also separate from each other. This extra, reductional step of meiosis is essential to sexual reproduction. Without meiosis, the chromo ...
... Meiosis, like mitosis, is also a necessary part of cell division. However, in meiosis not only do sister chromatids separate from each other, homologous chromosomes also separate from each other. This extra, reductional step of meiosis is essential to sexual reproduction. Without meiosis, the chromo ...
Platelet-Derived Growth Factor Receptor Activation Promotes the
... bind polypeptide ligands, mainly growth factors. Among the 58 RTK family members, epidermal growth factor receptor, plateletderived growth factor (PDGF) receptor (PDGFR), fibroblast growth factor receptor, vascular endothelial growth factor receptor, hepatocyte growth factor receptor (c-Met), and st ...
... bind polypeptide ligands, mainly growth factors. Among the 58 RTK family members, epidermal growth factor receptor, plateletderived growth factor (PDGF) receptor (PDGFR), fibroblast growth factor receptor, vascular endothelial growth factor receptor, hepatocyte growth factor receptor (c-Met), and st ...
Built to rebuild: in search of organizing principles in plant
... shoot meristem are patterned before the establishment of an active stem cell niche, as in the root tip excision experiments. Neither the shoot nor the root tip regeneration experiments rule out the existence of a central organizer but they suggest that at least part of meristem repatterning can be i ...
... shoot meristem are patterned before the establishment of an active stem cell niche, as in the root tip excision experiments. Neither the shoot nor the root tip regeneration experiments rule out the existence of a central organizer but they suggest that at least part of meristem repatterning can be i ...
Tolerance of Immobilized Yeast Cells in Imidazolium
... imidazolium-based ILs are biocompatible with yeast cells. The activity of yeast cells decreases with the increase of R-chain. [BMim][PF6] possessed the best biocompatibility compared to the other ILs. The relative activity retention of yeast cells pretreated with [BMim][PF6] for 12 h reached up to 7 ...
... imidazolium-based ILs are biocompatible with yeast cells. The activity of yeast cells decreases with the increase of R-chain. [BMim][PF6] possessed the best biocompatibility compared to the other ILs. The relative activity retention of yeast cells pretreated with [BMim][PF6] for 12 h reached up to 7 ...
Translocation and Clustering of Endosomes and
... Movement of fluorescently labeled organelles was visualized in vivo by VEFM. The following combinations of filter sets were used for fluorescence microscopy: the N2.1 filter set for rhodamine (BP 515-560, RKP 580, LP 580) and the L2 filter set for fluorescein ~ P 450-500, RKP 510, BP 515560). To avo ...
... Movement of fluorescently labeled organelles was visualized in vivo by VEFM. The following combinations of filter sets were used for fluorescence microscopy: the N2.1 filter set for rhodamine (BP 515-560, RKP 580, LP 580) and the L2 filter set for fluorescein ~ P 450-500, RKP 510, BP 515560). To avo ...
Geranylgeranylated Proteins are Involved in the Regulation of
... RhoA have been implicated in the regulation of cell cycle progression through G1 phase of the cell cycle. Constitutively activated mutants of Rac-1, Cdc42, and RhoA caused G1 progression and stimulation of DNA synthesis in fibroblasts (20 – 22). Furthermore, expression of active forms of Rac-1, Cdc4 ...
... RhoA have been implicated in the regulation of cell cycle progression through G1 phase of the cell cycle. Constitutively activated mutants of Rac-1, Cdc42, and RhoA caused G1 progression and stimulation of DNA synthesis in fibroblasts (20 – 22). Furthermore, expression of active forms of Rac-1, Cdc4 ...
Molecular cloning, sequence analysis, and function of the intestinal
... cDNA ends (RACE) primers (Table 1) were then designed based on the contiged sequence. The 5´ and 3´ ends were obtained using the SMART RACE Technique, as previously described (Rajalingam et al., 2001). The resulting 5´ and 3´ end sequences were jointed with the previous partial sequences to construc ...
... cDNA ends (RACE) primers (Table 1) were then designed based on the contiged sequence. The 5´ and 3´ ends were obtained using the SMART RACE Technique, as previously described (Rajalingam et al., 2001). The resulting 5´ and 3´ end sequences were jointed with the previous partial sequences to construc ...
PDF
... random and phasic models require RPCs to possess multiple competence states simultaneously, whereas the stepwise model suggests that an RPC adopts a single competence state at a given time to allow the production of a specific cell type. Besides ordered cell genesis, these models make different pred ...
... random and phasic models require RPCs to possess multiple competence states simultaneously, whereas the stepwise model suggests that an RPC adopts a single competence state at a given time to allow the production of a specific cell type. Besides ordered cell genesis, these models make different pred ...
Sample preparation and analytical strategies for
... avoid the release of cell surface components. Proteins present in the cell culture media could be co-isolated (e.g. serum proteins) if cells have not been properly washed prior to cell lysis and protein extraction. Care must be taken during cell harvesting to avoid triggering phosphorylation events ...
... avoid the release of cell surface components. Proteins present in the cell culture media could be co-isolated (e.g. serum proteins) if cells have not been properly washed prior to cell lysis and protein extraction. Care must be taken during cell harvesting to avoid triggering phosphorylation events ...
Diatoms
... Most of the time, diatoms reproduce asexually by mitotic cell divisions. Each daughter cell receives half the cell wall of the original diatom and ...
... Most of the time, diatoms reproduce asexually by mitotic cell divisions. Each daughter cell receives half the cell wall of the original diatom and ...
Defining retinal progenitor cell competence in
... random and phasic models require RPCs to possess multiple competence states simultaneously, whereas the stepwise model suggests that an RPC adopts a single competence state at a given time to allow the production of a specific cell type. Besides ordered cell genesis, these models make different pred ...
... random and phasic models require RPCs to possess multiple competence states simultaneously, whereas the stepwise model suggests that an RPC adopts a single competence state at a given time to allow the production of a specific cell type. Besides ordered cell genesis, these models make different pred ...
floppy_disk_drive 1615914
... effect of cross-talk between tracks and minimize the errors induced by minor run out problems on the diskette or diskette drive. Head alignment: Alignment is the process of placement of the heads with respect to the track that they must read and write. Head alignment can be checked only against some ...
... effect of cross-talk between tracks and minimize the errors induced by minor run out problems on the diskette or diskette drive. Head alignment: Alignment is the process of placement of the heads with respect to the track that they must read and write. Head alignment can be checked only against some ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.