Molecular profiling of single circulating tumor cells with diagnostic
... are lacking for comprehensive molecular characterization of CTCs with diagnostic precision. We therefore combined a workflow for enrichment and isolation of pure CTCs with a non-random whole genome amplification method for single cells and applied it to 510 single CTCs and 189 leukocytes of 66 CTC-p ...
... are lacking for comprehensive molecular characterization of CTCs with diagnostic precision. We therefore combined a workflow for enrichment and isolation of pure CTCs with a non-random whole genome amplification method for single cells and applied it to 510 single CTCs and 189 leukocytes of 66 CTC-p ...
final1-fp7-people-ief-2015-huber-final-report-02
... with the lamellipodial actin network in migrating cells as well as in neuronal growth cones. First results indicate that MTs are surprisingly sensitive to the presence of dense actin meshworks. In summary, the results obtained deliver a deeper understanding of MT growth dynamics as well as the inter ...
... with the lamellipodial actin network in migrating cells as well as in neuronal growth cones. First results indicate that MTs are surprisingly sensitive to the presence of dense actin meshworks. In summary, the results obtained deliver a deeper understanding of MT growth dynamics as well as the inter ...
KIR2DS1-Positive NK Cells Mediate Alloresponse against the C2
... IU/ml IL-2. NK clones were replated into 48-well plates along with additional feeders as described above and allowed to grow until an adequate numbers of cells were reached. Clones were then characterized for receptor phenotype by FACS. BLCLs were either obtained from the International Histocompatib ...
... IU/ml IL-2. NK clones were replated into 48-well plates along with additional feeders as described above and allowed to grow until an adequate numbers of cells were reached. Clones were then characterized for receptor phenotype by FACS. BLCLs were either obtained from the International Histocompatib ...
pdf - Penn State University
... other hemicelluloses are found tightly bound to cellulose, and they may also help anchor the microfibril to the matrix (reviewed in Cosgrove, 1997). Binding studies (Hayashi, 1989) suggest that xyloglucan does not simply bind to the microfibril surface, but much of it may also be entrapped within th ...
... other hemicelluloses are found tightly bound to cellulose, and they may also help anchor the microfibril to the matrix (reviewed in Cosgrove, 1997). Binding studies (Hayashi, 1989) suggest that xyloglucan does not simply bind to the microfibril surface, but much of it may also be entrapped within th ...
Text - Enlighten: Publications
... The protocol developed here for live labeling of root hairs is both simple and convenient, and is outlined in Figure 1. The elimination of fixation, dehydration and embedding is advantageous especially for handling such fragile specimens as root hairs. This methodology also does not require sectioni ...
... The protocol developed here for live labeling of root hairs is both simple and convenient, and is outlined in Figure 1. The elimination of fixation, dehydration and embedding is advantageous especially for handling such fragile specimens as root hairs. This methodology also does not require sectioni ...
Extended Survivability of Prostate Cancer Cells
... liferation, cell survival, and cell death. Cell proliferation is driven and regulated by various peptide growth factors and/or cytokines. Cell survival represents the cell's intrinsic life expectancy in the absence of survival factors, many of which overlap with the growth factors/ cytokines that dr ...
... liferation, cell survival, and cell death. Cell proliferation is driven and regulated by various peptide growth factors and/or cytokines. Cell survival represents the cell's intrinsic life expectancy in the absence of survival factors, many of which overlap with the growth factors/ cytokines that dr ...
dextran by suspension-cultured plant cells
... protoplasts were also incubated in the appropriate medium containing 1 % ethanol only, as above. Following treatment, samples of cells and protoplasts were washed and mounted for fluorescence microscopy. Fluorescent dextrans. Samples of 3- to 9-day-old cell suspensions (5xlO 6 cells ml" 1 ) were tre ...
... protoplasts were also incubated in the appropriate medium containing 1 % ethanol only, as above. Following treatment, samples of cells and protoplasts were washed and mounted for fluorescence microscopy. Fluorescent dextrans. Samples of 3- to 9-day-old cell suspensions (5xlO 6 cells ml" 1 ) were tre ...
Targeting Robo4-dependent slit signaling to survive the cytokine storm in sepsis and influenza. Science Trans Med 2, 1-9.
... immune responses. The marked and abrupt release of multiple cytokines by the immune system, often referred to as hypercytokinemia or cytokine storm, itself has disruptive effects on the host’s physiology. In many infections, components of the resulting cytokine-induced secondary inflammatory injury ...
... immune responses. The marked and abrupt release of multiple cytokines by the immune system, often referred to as hypercytokinemia or cytokine storm, itself has disruptive effects on the host’s physiology. In many infections, components of the resulting cytokine-induced secondary inflammatory injury ...
RNA interference screening in Drosophila primary cells for genes
... obtained from Drosophila gastrulating embryos carrying rp298-lacZ immediately following plating. Cells are stained using DAPI for nuclei (A, and blue in C), and antibodies targeting Dmef2 (B, and green in C,E), β-galactosidase (D, and red in E,F) and Lmd (green in F). (G-I) Primary cells derived fro ...
... obtained from Drosophila gastrulating embryos carrying rp298-lacZ immediately following plating. Cells are stained using DAPI for nuclei (A, and blue in C), and antibodies targeting Dmef2 (B, and green in C,E), β-galactosidase (D, and red in E,F) and Lmd (green in F). (G-I) Primary cells derived fro ...
Golgi complex
... The Golgi complex distributes substances to other parts of the cell. membrane bound structure. The Golgi complex transports nutrients and waste by breaking of part of itself in bubbles .which transports the nutrients and waste to other parts of the cell, and out side of the cell with the bubble even ...
... The Golgi complex distributes substances to other parts of the cell. membrane bound structure. The Golgi complex transports nutrients and waste by breaking of part of itself in bubbles .which transports the nutrients and waste to other parts of the cell, and out side of the cell with the bubble even ...
Actin-based motility of endosomes is linked to the polar tip growth of
... movements of endocytic compartments are accomplished along microtubules, whereas the actin cytoskeleton is responsible for short-distance movements (Goode et al., 2000). Important roles for endocytosis during polarized root hair expansion, which is restricted to the tips of these cells, have been po ...
... movements of endocytic compartments are accomplished along microtubules, whereas the actin cytoskeleton is responsible for short-distance movements (Goode et al., 2000). Important roles for endocytosis during polarized root hair expansion, which is restricted to the tips of these cells, have been po ...
Evolution and Diversity of Plant Cell Walls: From Algae to Flowering
... playing a key role in their environmental interactions. This has resulted in compositional variation that is dependent on developmental stage, cell type, and season. Further variation is evident that has a phylogenetic basis. Plants and algae have a complex phylogenetic history, including acquisitio ...
... playing a key role in their environmental interactions. This has resulted in compositional variation that is dependent on developmental stage, cell type, and season. Further variation is evident that has a phylogenetic basis. Plants and algae have a complex phylogenetic history, including acquisitio ...
Full PDF
... A hallmark of tissue injury and repair is the turnover of extracellular matrix components. This review focuses on the role of the glycosaminoglycan hyaluronan in tissue injury and repair. Both the synthesis and degradation of extracellular matrix are critical contributors to tissue repair and remode ...
... A hallmark of tissue injury and repair is the turnover of extracellular matrix components. This review focuses on the role of the glycosaminoglycan hyaluronan in tissue injury and repair. Both the synthesis and degradation of extracellular matrix are critical contributors to tissue repair and remode ...
Symbiotic Nitrogen Fixation
... from the plant plasmalemma (Figure 1). Because research on legume symbiosis is the most advanced of these three symbiotic systems, in this article we concentrate mainly on this system. The interaction of rhizobia and legumes begins with signal exchange and recognition of the symbiotic partners, foll ...
... from the plant plasmalemma (Figure 1). Because research on legume symbiosis is the most advanced of these three symbiotic systems, in this article we concentrate mainly on this system. The interaction of rhizobia and legumes begins with signal exchange and recognition of the symbiotic partners, foll ...
Cell-surface location of Listeria-specific protein p60
... anti-p60 antiserum. Proteins immunologically related to p60 with a similar molecular mass were detected in all strains except L. grayi and L. murrayi (data not shown). In the case of L. seeligeri and L. welshimeri, however, only a weak reaction was observed. Production of p60 by L. monocytogenes ATC ...
... anti-p60 antiserum. Proteins immunologically related to p60 with a similar molecular mass were detected in all strains except L. grayi and L. murrayi (data not shown). In the case of L. seeligeri and L. welshimeri, however, only a weak reaction was observed. Production of p60 by L. monocytogenes ATC ...
Chromosome Dynamics in Meiotic Prophase I in
... Processes taking place in early meiotic prophase I impose major changes on the appearance and behavior of chromosomes. Thin, thread-like univalent chromosomes present at the beginning of zygotene thicken as a result of progressing chromatin condensation and form bivalents as a result of homologous p ...
... Processes taking place in early meiotic prophase I impose major changes on the appearance and behavior of chromosomes. Thin, thread-like univalent chromosomes present at the beginning of zygotene thicken as a result of progressing chromatin condensation and form bivalents as a result of homologous p ...
Plastid Ontogeny during Petal Development in Arabidopsis
... mature petal (Fig. 2) showed that these two types of epidermal cells each occupied about 50% of the petal length and that the point of transition of epidermal cell shape is quite marked. The elongate morphology of the basal epidermal cells relates to their rapid expansion, which pushes the petal out ...
... mature petal (Fig. 2) showed that these two types of epidermal cells each occupied about 50% of the petal length and that the point of transition of epidermal cell shape is quite marked. The elongate morphology of the basal epidermal cells relates to their rapid expansion, which pushes the petal out ...
Dynamic Organization of DNA Replication in Mammalian Cell Nuclei
... within the nucleus by folding in a specific manner and by contacting the nuclear surface at specific sites (Agard and Sedat, 1983 ; Mathog et al ., 1984; Hochstrasser et al., 1986; Hochstrasser and Sedat, 1987a,b) . Often, the disposition of the nucleolus is characteristic of the cell type . In epit ...
... within the nucleus by folding in a specific manner and by contacting the nuclear surface at specific sites (Agard and Sedat, 1983 ; Mathog et al ., 1984; Hochstrasser et al., 1986; Hochstrasser and Sedat, 1987a,b) . Often, the disposition of the nucleolus is characteristic of the cell type . In epit ...
Ontogeny of erythropoiesis
... switching is regulated by altered transcription factor presence instead of chromatin accessibility [25]. The GATA1 transcription factor plays an essential role in the regulation of erythroid-specific genes in both primitive and definitive erythroid cells and the loss of GATA1 leads to the arrest of ...
... switching is regulated by altered transcription factor presence instead of chromatin accessibility [25]. The GATA1 transcription factor plays an essential role in the regulation of erythroid-specific genes in both primitive and definitive erythroid cells and the loss of GATA1 leads to the arrest of ...
Structural Requirements of Simple Organic
... We have previously reported that the cationic charge of xanthene dyes was critical for their differential accumulation in certain normal versus tumor epithelial cell lines (8-10). It was subsequently shown that the normal epithelial cell line (CV-1) that had reduced accumulation of these cationic xa ...
... We have previously reported that the cationic charge of xanthene dyes was critical for their differential accumulation in certain normal versus tumor epithelial cell lines (8-10). It was subsequently shown that the normal epithelial cell line (CV-1) that had reduced accumulation of these cationic xa ...
Using monoclonal antibodies to label living root hairs: a novel tool
... The protocol developed here for live labeling of root hairs is both simple and convenient, and is outlined in Figure 1. The elimination of fixation, dehydration and embedding is advantageous especially for handling such fragile specimens as root hairs. This methodology also does not require sectioni ...
... The protocol developed here for live labeling of root hairs is both simple and convenient, and is outlined in Figure 1. The elimination of fixation, dehydration and embedding is advantageous especially for handling such fragile specimens as root hairs. This methodology also does not require sectioni ...
The proteasomal deubiquitinating enzyme POH1 promotes the
... Received: 22 May 2012; accepted: 26 July 2012; published online: 21 August 2012 ...
... Received: 22 May 2012; accepted: 26 July 2012; published online: 21 August 2012 ...
14 March 2017 ABI Response to FCA CP17/3: Proposed Handbook
... We are supportive of HM Treasury, the PRA and the FCA’s attempts to create a regulatory framework to attract Insurance Linked Security (ILS) business to the UK and are broadly supportive of the Handbook changes outlined by the FCA in CP17/3. Although supportive, we have some concerns with the Handbo ...
... We are supportive of HM Treasury, the PRA and the FCA’s attempts to create a regulatory framework to attract Insurance Linked Security (ILS) business to the UK and are broadly supportive of the Handbook changes outlined by the FCA in CP17/3. Although supportive, we have some concerns with the Handbo ...
Involvement of reactive oxygen species in lanthanum
... Millipore-filtered deionized water and briefly centrifuging the solution, the contents of Mn, Zn, Cu, Fe, K and Ca were determined using inductively coupled plasma atomic emission spectroscopy (ICP-AES). Each experiment was repeated six times. Statistical analysis At least 20 roots were analysed for ...
... Millipore-filtered deionized water and briefly centrifuging the solution, the contents of Mn, Zn, Cu, Fe, K and Ca were determined using inductively coupled plasma atomic emission spectroscopy (ICP-AES). Each experiment was repeated six times. Statistical analysis At least 20 roots were analysed for ...
Supplement
... For the first three distributions we need to determine either the fixed or initial probability p or the threshold parameter t. We choose parameters for these three models such that the percentage of errors (two neighboring cells becoming SOPs in the same round) is roughly 10%. The resulting paramete ...
... For the first three distributions we need to determine either the fixed or initial probability p or the threshold parameter t. We choose parameters for these three models such that the percentage of errors (two neighboring cells becoming SOPs in the same round) is roughly 10%. The resulting paramete ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.