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Diffusion and Osmosis Investigations
Diffusion and Osmosis Investigations

... solution on the other side of the membrane; water will move down its concentration gradient into the other solution. Isotonic solutions have equal water potentials. In nonwalled cells, such as animal cells, the movement of water into and out of a cell is affected by the relative solute concentration ...
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PDF

... Finally, in a third experiment of this series, either the 0 teloblast or its sister teloblast P was injected as usual with HRP and fast green dye in 34 stage 6c embryos. At stage 7, the injected teloblast could still be identified by its green color, at which time the uninjected ipsilateral P or 0 t ...
Cell crawling mediates collective cell migration to
Cell crawling mediates collective cell migration to

... the emergence of lamellipodium surrounding the gap (Fig. 2B). According to previous studies, it was suggested that purse-string contraction repaired small epithelial wounds (4, 5), whereas larger wounds induced also cell crawling with formation of lamellipodia (6, 7, 23). Surprisingly, the presence ...
Protein import into the nucleus - Universitätsklinikum des Saarlandes
Protein import into the nucleus - Universitätsklinikum des Saarlandes

Chapter 2 Role of the synthase domain of Ags1p in cell wall α
Chapter 2 Role of the synthase domain of Ags1p in cell wall α

... Distinct plasma membrane-localised synthases are responsible for the production of structural polysaccharides in the fungal cell wall, mostly (1,3)-β-glucan, chitin, and α-glucan. (1,3)-β-Glucan and chitin synthases were identified first in the budding yeast Saccharomyces cerevisiae (Douglas et al., 1 ...
A Cell Type-specific Constitutive Point Mutant of the
A Cell Type-specific Constitutive Point Mutant of the

... module; PCR, polymerase chain reaction; kb, kilobase pair(s); wt, wild type; HSV, herpes simplex virus. This paper is available on line at http://www.jbc.org ...
Structural development and bioactive content of red bulb plant
Structural development and bioactive content of red bulb plant

... significantly different. Cell elongation is a part of cell extension in which growth tends to occur towards one direction, usually along the plant axis (Fosket 1994). The number of stomata in lower epidermis differed significantly only between T1 (177.78/mm2) and T2 (262.22/mm2), while later observa ...
DNA Methylation Profiles Define Stem Cell Identity and
DNA Methylation Profiles Define Stem Cell Identity and

... profiles identify a cohort of developmentally regulated sequence elements, such as orphan CpG islands, that will be most valuable to uncover novel transcriptional regulators and pivotal ‘‘gatekeeper’’ genes in pluripotency and lineage differentiation. STEM CELLS 2012;30:2732–2745 ...
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... Although there is ever-increasing data to support meniscus allograft transplantation and synthetic meniscus scaffold implantation, a successful outcome for these surgical procedures is not universal. Although allograft material may contain some endogenous cells, the implantation of synthetic scaffol ...
Simple microwave field imaging technique using hot atomic vapor
Simple microwave field imaging technique using hot atomic vapor

Microtechnologies for Cell Microenvironment Control and
Microtechnologies for Cell Microenvironment Control and

... During the last two decades, we have witnessed a number of key developments in the area of the microtechnologies, which allows introducing control and complexity over a full range of environmental factor at the microscale level. For example, technologies for the accurate structuration of surfaces fo ...
Inflammation near the Nerve Cell Body
Inflammation near the Nerve Cell Body

... sensoryaxons (McQuarrie et al., 1977; Richardson and Verge, 1987). The present observations suggestthat similar reactions can be elicited by more direct stimulation of the nerve cell body. Injection into the DRG of either macrophagesor the inflammatory agent C. parvum significantly enhancesaxonal re ...
Isolation and Characterization of Conditional-Lethal Mutations in the TUB1 alpha-Tubulin Gene of the Yeast Saccharomyces cerevisiae .
Isolation and Characterization of Conditional-Lethal Mutations in the TUB1 alpha-Tubulin Gene of the Yeast Saccharomyces cerevisiae .

... demonstrated the role of microtubules in chromothat are components of yeast microtubules (SCHATZet some separation on spindles and in nuclear movemental. 1986). T h e functional differences between these during mitoticgrowthandmating (DELGADOand two genes have been examined through the construcCONDE ...
BIOL 1230 Biology I Course Master Syllabus July 23 2012
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... proportion of elements found in living things. Describe the properties of carbon and the basic ways organic molecules are constructed 3. Describe the basic chemical and physical properties of water that make it essential for life 4. Name and describe the principle properties of lipids, proteins, ca ...
Enzymes
Enzymes

... • Denatured ...
Single-Cell Isolation and Gene Analysis: Pitfalls and
Single-Cell Isolation and Gene Analysis: Pitfalls and

... PCR in real-time (i.e., at each cycle), however, it became possible to calculate the starting amount of the DNA template based on the exponential phase of the PCR curve. Additionally, using the exponential phase, rather than the plateau phase, increased the dynamic range. Currently, real-time PCR an ...
Cell Proliferation Kinetics and Drug Sensitivity of
Cell Proliferation Kinetics and Drug Sensitivity of

... exponentially with a generation time of about 12 hr. Expo nential growth was maintained until a cell density of 4 x 10@ In 1966, Bruce et al. (8) compared the drug sensitivity of cells/mI was reached; stationary I cultures had 6 x 10@ cells/mI, and stationary II cultures had about 8 x 10@to 106 rapi ...
Heat Shock Response and Protein Degradation
Heat Shock Response and Protein Degradation

The Role of Lipid Domains in Bacterial Cell Processes
The Role of Lipid Domains in Bacterial Cell Processes

... membrane localization and its signal was diffusely distributed throughout the cytoplasm. This failure of MinD to localize to the membrane correlated with loss of MinCD’s function as a cell division inhibitor. The PG helical domains may also serve as cues for the localization of other proteins with s ...
Cell Energetics
Cell Energetics

... Cell Energetic Respiration is a process used by plants and animals to create energy for the cells in the body to use. In respiration, glucose in the cells is broken down into smaller units of energy called ATP. The ATP acts as a battery that can plug into the cell and provide it with energy. There a ...
Autophagic Components Contribute to Hypersensitive Cell Death in
Autophagic Components Contribute to Hypersensitive Cell Death in

How migration occurs
How migration occurs

... Cycle between a GDP-bound, inactive form GTP-bound, active form. The cycle is regulated by ...


... physiological functions of ubiquitin-mediated proteolysis, Hershko et al. devised an immunochemical method for isolating ubiquitin-protein conjugates from intact cells (Hershko et al., 1982). Cells were first pulse-labelled with tryptophan, an amino acid which is missing in ubiquitin. Using an antib ...
Life: The Science of Biology, 9e
Life: The Science of Biology, 9e

... Mammalian cells take in cholesterol by receptor-mediated endocytosis. In the liver, cholesterol is packaged into low-density lipoprotein, or LDL, and secreted to the bloodstream. Cells that need cholesterol have receptors for the LDLs in clathrin-coated pits. ...
Gumbo JR and Cloete TE
Gumbo JR and Cloete TE

... cell death upon exposure to B. mycoides i.e. cell membrane lysis and shadowing of algal cells leading to photo-inhibition. There were ultra-structural changes that occurred in bacteria treated M. aeruginosa cells. SEM images showed swollen M. aeruginosa cells due to cell membrane damage and increase ...
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Mitosis



Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.
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