The AMF-R tubule is a smooth ilimaquinone
... (Benlimame et al., 1995), in IQ treated cells AMF-R labels rough ER tubules as well as tubules which exhibit both rough and smooth portions (Fig. 7). Of particular interest is the fact that while IQ does not affect the rough portion of the tubule, the smooth portion presents a morphology of intercon ...
... (Benlimame et al., 1995), in IQ treated cells AMF-R labels rough ER tubules as well as tubules which exhibit both rough and smooth portions (Fig. 7). Of particular interest is the fact that while IQ does not affect the rough portion of the tubule, the smooth portion presents a morphology of intercon ...
Lenti-X™ Tet-On® Advanced Expression System User
... binding to the TRE in PTight; thus basal expression is often undetectable. • Highly specific. The binding of the Tet-Advanced TetR-derived domains to the tetO target sequences of PTight is highly specific and does not activate off-target cellular genes. This high degree of specificity may be due in ...
... binding to the TRE in PTight; thus basal expression is often undetectable. • Highly specific. The binding of the Tet-Advanced TetR-derived domains to the tetO target sequences of PTight is highly specific and does not activate off-target cellular genes. This high degree of specificity may be due in ...
unresponsive to cell division control by polypeptide mating hormone
... control the differentiation of cell type and one gene, stet, that functions exclusively in MA Ta cells to mediate responsiveness to polypeptide hormone . In most eukaryotic cells for which information exists, division is controlled in G 1, before the initiation of DNA synthesis, whether by hormones, ...
... control the differentiation of cell type and one gene, stet, that functions exclusively in MA Ta cells to mediate responsiveness to polypeptide hormone . In most eukaryotic cells for which information exists, division is controlled in G 1, before the initiation of DNA synthesis, whether by hormones, ...
PDF
... competence states simultaneously, whereas the stepwise model suggests that an RPC adopts a single competence state at a given time to allow the production of a specific cell type. Besides ordered cell genesis, these models make different predictions concerning the stage of cell production of individ ...
... competence states simultaneously, whereas the stepwise model suggests that an RPC adopts a single competence state at a given time to allow the production of a specific cell type. Besides ordered cell genesis, these models make different predictions concerning the stage of cell production of individ ...
Article
... transferred to the ITO (in EB-based cell) or to the conduction band edge of Nc-TiO2 and then to the ITO (in EB:Nc-TiO 2-based cell). In the mean time, holes can be transferred from (EB) + to Eu 2+. The Eu 2+ is recovered at the counter electrode where Eu3+ accepts electrons from the external circuit ...
... transferred to the ITO (in EB-based cell) or to the conduction band edge of Nc-TiO2 and then to the ITO (in EB:Nc-TiO 2-based cell). In the mean time, holes can be transferred from (EB) + to Eu 2+. The Eu 2+ is recovered at the counter electrode where Eu3+ accepts electrons from the external circuit ...
The IML3/ MCM19 gene of Saccharomyces cerevisiae is required
... was designed in the ATG primer (the primer used to amplify the ORF from its ®rst codon), so that the BamHI site of pGBT9 or pGAD424 could be used for fusing the ORF in frame with the binding or activation domains of these plasmids. For CHL4/ MCM17, the plasmid pGK8, carrying the CHL4/MCM17 gene (Roy ...
... was designed in the ATG primer (the primer used to amplify the ORF from its ®rst codon), so that the BamHI site of pGBT9 or pGAD424 could be used for fusing the ORF in frame with the binding or activation domains of these plasmids. For CHL4/ MCM17, the plasmid pGK8, carrying the CHL4/MCM17 gene (Roy ...
Defining retinal progenitor cell competence in
... competence states simultaneously, whereas the stepwise model suggests that an RPC adopts a single competence state at a given time to allow the production of a specific cell type. Besides ordered cell genesis, these models make different predictions concerning the stage of cell production of individ ...
... competence states simultaneously, whereas the stepwise model suggests that an RPC adopts a single competence state at a given time to allow the production of a specific cell type. Besides ordered cell genesis, these models make different predictions concerning the stage of cell production of individ ...
Cell cycle regulation by the bacterial nucleoid
... inherit an intact copy of the genome. Bacteria typically contain a single circular chromosome that is replicated bidirectionally from a single origin of replication (oriC; 08). During replication the newly synthesised sister chromosomes rapidly segregate ‘origin-first’ in opposite directions, before ...
... inherit an intact copy of the genome. Bacteria typically contain a single circular chromosome that is replicated bidirectionally from a single origin of replication (oriC; 08). During replication the newly synthesised sister chromosomes rapidly segregate ‘origin-first’ in opposite directions, before ...
Supporting Online Material for
... direct the synthesis of shRNAs targeting LacZ or Fbxl3, as indicated. In this experiment the shRNA construct 3 to mouse Fbxl3 (see fig. S4A) was used. Cells were asynchronously grown to confluence in a medium containing 10% fetal bovine serum (Asynch.). Asynchronous cells were serum starved for 16 h ...
... direct the synthesis of shRNAs targeting LacZ or Fbxl3, as indicated. In this experiment the shRNA construct 3 to mouse Fbxl3 (see fig. S4A) was used. Cells were asynchronously grown to confluence in a medium containing 10% fetal bovine serum (Asynch.). Asynchronous cells were serum starved for 16 h ...
Methanopyrus (Methanopyrus kandleri)
... usually in the scum that collects on the top of the water. Paramecia are safe from most things that would eat them in the pond scum, and they are able to find things to eat! Paramecia eat bacteria, other unicellular organisms and decaying plant matter. They are able to take in the oxygen (O2) that i ...
... usually in the scum that collects on the top of the water. Paramecia are safe from most things that would eat them in the pond scum, and they are able to find things to eat! Paramecia eat bacteria, other unicellular organisms and decaying plant matter. They are able to take in the oxygen (O2) that i ...
Strategies and New Developments in the Generation of Patient-Specific Pluripotent Stem Cells Yamanaka, REVIEW (2007) Cell Stem Cell 1. July 2007 pp 39- 49.
... embryos with germline transmission. Currently, this method has only been demonstrated with mouse zygotes. However, it does raise the possibility that discarded human IVF embryos could potentially be used as recipients for human ntES cell derivation instead of oocytes and even, hypothetically, that m ...
... embryos with germline transmission. Currently, this method has only been demonstrated with mouse zygotes. However, it does raise the possibility that discarded human IVF embryos could potentially be used as recipients for human ntES cell derivation instead of oocytes and even, hypothetically, that m ...
ochromonas malhamensis
... the cytochemical study . During starvation, the acid hydrolase specific activities were consistently twice those found in cells grown in an osmotrophic complete medium . Ochromonas fed E. coli showed no increase in acid hydrolase specific activity as compared to controls not fed E. coli. The latency ...
... the cytochemical study . During starvation, the acid hydrolase specific activities were consistently twice those found in cells grown in an osmotrophic complete medium . Ochromonas fed E. coli showed no increase in acid hydrolase specific activity as compared to controls not fed E. coli. The latency ...
ID helix-loop-helix proteins - Journal of Cell Science
... Finally, Kondo and Raff (Kondo and Raff, 2000) have recently implicated ID4, which has been less well studied than its relatives, in the ‘molecular clock’ mechanism controlling the timing of oligodendrocyte differentiation. The above studies clearly illustrate the complex role played by ID proteins ...
... Finally, Kondo and Raff (Kondo and Raff, 2000) have recently implicated ID4, which has been less well studied than its relatives, in the ‘molecular clock’ mechanism controlling the timing of oligodendrocyte differentiation. The above studies clearly illustrate the complex role played by ID proteins ...
Metabolic Incorporation of Stable Isotope Labels into Glycans
... during their existence1,3 and virtually all membrane and secreted proteins are glycosylated.2 Glycoprotein glycans often play crucial roles in physiological events such as cell-cell recognition,4-6 signal transduction,7 inflammation8 and tumorigenesis.9-13 Given the important physiological roles of ...
... during their existence1,3 and virtually all membrane and secreted proteins are glycosylated.2 Glycoprotein glycans often play crucial roles in physiological events such as cell-cell recognition,4-6 signal transduction,7 inflammation8 and tumorigenesis.9-13 Given the important physiological roles of ...
PIERGIORGIO PETTAZZONI
... proliferation and differentiation. One micromolar HNE, a dose similar to those detected in normal cells, inhibits proliferation and induces differentiation of HL-60 human leukemic cells. This HNE concentration also induces erythroid differentiation by increasing gamma globin expression in MEL and K5 ...
... proliferation and differentiation. One micromolar HNE, a dose similar to those detected in normal cells, inhibits proliferation and induces differentiation of HL-60 human leukemic cells. This HNE concentration also induces erythroid differentiation by increasing gamma globin expression in MEL and K5 ...
Full article - Journal of Integrative Bioinformatics
... Two-component systems (TCS) are the most prevalent gene regulatory mechanism in bacteria. A typical TCS is comprised of a histidine kinase (HK) and a partner response regulator (RR). Specific environment signals lead to autophosphorylation of different HKs, which in turn act as phosphoryl donors for ...
... Two-component systems (TCS) are the most prevalent gene regulatory mechanism in bacteria. A typical TCS is comprised of a histidine kinase (HK) and a partner response regulator (RR). Specific environment signals lead to autophosphorylation of different HKs, which in turn act as phosphoryl donors for ...
Recurrences in Thom spectra
... Any class α ∈ π∗ S must be represented by some element (plus indeterminacy) on the E 1 -page, and that element is called its root invariant R(α). Many interesting basic calculations of the root invariant are known, and it appears to have “redshifting properties” in the language of chromatic homotopy ...
... Any class α ∈ π∗ S must be represented by some element (plus indeterminacy) on the E 1 -page, and that element is called its root invariant R(α). Many interesting basic calculations of the root invariant are known, and it appears to have “redshifting properties” in the language of chromatic homotopy ...
review - Nature
... PKC-activating phorbol ester treatment to induce Fas-resistance was examined. Phorbol ester produced only partial protection against Fas-mediated apoptosis, whereas the combination of a phorbol ester plus a calcium ionophore brought about virtually complete Fas-resistance, the level of which was as ...
... PKC-activating phorbol ester treatment to induce Fas-resistance was examined. Phorbol ester produced only partial protection against Fas-mediated apoptosis, whereas the combination of a phorbol ester plus a calcium ionophore brought about virtually complete Fas-resistance, the level of which was as ...
Co-Infection with Mycobacterium tuberculosis Impairs HIV
... with HIV and Mycobacterium tuberculosis (MTB) increases disease progression of both diseases[1]. For example, higher HIV viral loads are observed in MTB co-infection and increased HIV replication occurs in MTB infected macrophages [2, 3]. The high levels of inflammation and immune activation, as pre ...
... with HIV and Mycobacterium tuberculosis (MTB) increases disease progression of both diseases[1]. For example, higher HIV viral loads are observed in MTB co-infection and increased HIV replication occurs in MTB infected macrophages [2, 3]. The high levels of inflammation and immune activation, as pre ...
Roles of vaccinia virus EEV-specific proteins in intracellular actin tail
... appeared a little sooner with ∆B5R and ∆A34R, a similar proportion of cells had developed cellular projections by 18 h p.i. ∆A36R forms both IEV and CEV particles ...
... appeared a little sooner with ∆B5R and ∆A34R, a similar proportion of cells had developed cellular projections by 18 h p.i. ∆A36R forms both IEV and CEV particles ...
A Heat Shows of Two Shock-resistant Mutant of Saccharomyces
... isolated at the mutation frequency of 10-7 from a culture treated with ethyl methane sulfonate. Cells of the mutant are approximately 1,000-fold more resistant to lethal heat shock than those of the parental strain. Tetrad analysis indicates that phenotypes revealed by this mutant segregated togethe ...
... isolated at the mutation frequency of 10-7 from a culture treated with ethyl methane sulfonate. Cells of the mutant are approximately 1,000-fold more resistant to lethal heat shock than those of the parental strain. Tetrad analysis indicates that phenotypes revealed by this mutant segregated togethe ...
Review The Role of Laminin in Embryonic Cell Polarization and
... Metazoan embryogenesis comprises a sequence of events that results in the generation of differentiated cells and in the organization of those cells within the organism. One of the fundamental processes involved in both differentiation and organization is the establishment of cell polarity. This cell ...
... Metazoan embryogenesis comprises a sequence of events that results in the generation of differentiated cells and in the organization of those cells within the organism. One of the fundamental processes involved in both differentiation and organization is the establishment of cell polarity. This cell ...
Differential requirement for OBF-1 during antibody
... We examined the cell-surface phenotypes of OBF-1/ and heterozygous control cells in more detail, staining simultaneously for Syndecan-1 and other markers known to change during ASC differentiation. In control cultures, Syndecan-1hi ASC lost the expression of CD25, Fas, and FcRII, but Syndecan-1in ...
... We examined the cell-surface phenotypes of OBF-1/ and heterozygous control cells in more detail, staining simultaneously for Syndecan-1 and other markers known to change during ASC differentiation. In control cultures, Syndecan-1hi ASC lost the expression of CD25, Fas, and FcRII, but Syndecan-1in ...
Examination of the endosomal and lysosomal pathways in
... in the membranes of both the lysosomal and contractile vacuole systems, are altered in both endocytosis and osmoregulation (Bush et al., unpublished data). The transport of material to and from lysosomes requires the proper movement and fusion of membrane vesicles, and the transit of these membrane- ...
... in the membranes of both the lysosomal and contractile vacuole systems, are altered in both endocytosis and osmoregulation (Bush et al., unpublished data). The transport of material to and from lysosomes requires the proper movement and fusion of membrane vesicles, and the transit of these membrane- ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.