The Heat-Shock Proteins
... antigen, MethA, has recently been identified as hsp90 (215). Although small portion of the protein is foundon the cell surface in this tumorline, it is postulated that it arrives there secondarily, by deposition of protein fromlysed cells. The ERprotein is larger than the cytosolic protein, with an ...
... antigen, MethA, has recently been identified as hsp90 (215). Although small portion of the protein is foundon the cell surface in this tumorline, it is postulated that it arrives there secondarily, by deposition of protein fromlysed cells. The ERprotein is larger than the cytosolic protein, with an ...
A Stable Serine Protease, Wrightin, from the Latex of the Plant
... and 25% glycerol was loaded on each gel and electrophoresed at a constant current of 2 mA per rod for 4 h. After the run, tube gels were fixed in fixing solution (30 mL methanol, 70 mL of distilled water, 3.45 g of sulfosalicylic acid, and 11.5 g of trichloroacetic acid in 100 mL of distilled water) ...
... and 25% glycerol was loaded on each gel and electrophoresed at a constant current of 2 mA per rod for 4 h. After the run, tube gels were fixed in fixing solution (30 mL methanol, 70 mL of distilled water, 3.45 g of sulfosalicylic acid, and 11.5 g of trichloroacetic acid in 100 mL of distilled water) ...
Peptides and proteins Chapter 36:
... are incorporated into peptides and proteins via the genetic code. Individually, these amino acids in isolation have no specific intrinsic biological activity. Within cells, they exist in a pool from which they can be called upon to make peptides and proteins or, sometimes, biogenic amines, such as s ...
... are incorporated into peptides and proteins via the genetic code. Individually, these amino acids in isolation have no specific intrinsic biological activity. Within cells, they exist in a pool from which they can be called upon to make peptides and proteins or, sometimes, biogenic amines, such as s ...
PDF
... rendered the enzyme soluble but had little impact on its catalytic parameters, indicating that CAAD’s function is mostly structural (Olmedo-Verd et al., 2011). Based on these data, it was proposed that in the other species where ValRS was also observed to contain CAAD, the enzyme would localize in t ...
... rendered the enzyme soluble but had little impact on its catalytic parameters, indicating that CAAD’s function is mostly structural (Olmedo-Verd et al., 2011). Based on these data, it was proposed that in the other species where ValRS was also observed to contain CAAD, the enzyme would localize in t ...
Application of a bacterial two-hybrid system for the
... adds alanine or serine to the stem peptide in cooperation with FibA (MurM) to form an Ala2 or Ala–Ser side chain (Filipe & Tomasz, 2000; Weber et al., 2000). FibB proved to be able to form homodimers, but not heterodimers with FibA, and FibA homodimerization was also not observed (data not shown). T ...
... adds alanine or serine to the stem peptide in cooperation with FibA (MurM) to form an Ala2 or Ala–Ser side chain (Filipe & Tomasz, 2000; Weber et al., 2000). FibB proved to be able to form homodimers, but not heterodimers with FibA, and FibA homodimerization was also not observed (data not shown). T ...
Price List - MitoSciences
... mAbs reacting specifically with the core protein 1, the Rieske protein, and a 10 kDa Complex III subunit of complex III respectively, along with a mAb against the subunit of Complex V (ATP synthase F1) included as a control. This mAb mixture can be used to probe the isolated complex III for integ ...
... mAbs reacting specifically with the core protein 1, the Rieske protein, and a 10 kDa Complex III subunit of complex III respectively, along with a mAb against the subunit of Complex V (ATP synthase F1) included as a control. This mAb mixture can be used to probe the isolated complex III for integ ...
Autophagy in the Eukaryotic Cell - CiteSeerX
... ubiquitin-like (UBL) systems that are essential for vesicle biogenesis (84). Ubiquitin is first activated by binding to a ubiquitin-activating enzyme (also called E1). Then it is transferred to a ubiquitin-conjugating enzyme (also called E2). Then a ubiquitin protein ligase enzyme (also called E3) c ...
... ubiquitin-like (UBL) systems that are essential for vesicle biogenesis (84). Ubiquitin is first activated by binding to a ubiquitin-activating enzyme (also called E1). Then it is transferred to a ubiquitin-conjugating enzyme (also called E2). Then a ubiquitin protein ligase enzyme (also called E3) c ...
AKAP MEDIATED SIGNAL TRANSDUCTION Jennifer J. Carlisle
... Regulation of PKA by A-Kinase Anchoring Proteins Discrete localization of type II PKA within the cell is due chiefly to association of the regulatory subunit with nonenzymatic scaffolding proteins called A-kinase anchoring proteins (AKAPs) (reviewed in 19–22). The first AKAPs, MAP2 and AKAP75, were ...
... Regulation of PKA by A-Kinase Anchoring Proteins Discrete localization of type II PKA within the cell is due chiefly to association of the regulatory subunit with nonenzymatic scaffolding proteins called A-kinase anchoring proteins (AKAPs) (reviewed in 19–22). The first AKAPs, MAP2 and AKAP75, were ...
Current Topics Intrinsic Disorder and Protein Function†
... proteins (3). Tight regulation of intracellular protease activity can also be understood as a mechanism to protect proteasesensitive sites (5, 12). Additional mechanisms help disordered proteins avoid proteolysis in vivo. Some disordered regions are inaccessible to proteases due to steric factors; o ...
... proteins (3). Tight regulation of intracellular protease activity can also be understood as a mechanism to protect proteasesensitive sites (5, 12). Additional mechanisms help disordered proteins avoid proteolysis in vivo. Some disordered regions are inaccessible to proteases due to steric factors; o ...
Overexpression of yeast karyopherin Pse1p/Kap121p stimulates the
... Fig. 4. PSE1 or PSE1-t overexpression improves the mitochondrial delivery of the ABC transporter Atm1p. A. Mitochondrial proteins used in this study are distributed according to their mesohydrophobicity values (which is an average regional hydrophobicity value determined over a 60±80 residue window) ...
... Fig. 4. PSE1 or PSE1-t overexpression improves the mitochondrial delivery of the ABC transporter Atm1p. A. Mitochondrial proteins used in this study are distributed according to their mesohydrophobicity values (which is an average regional hydrophobicity value determined over a 60±80 residue window) ...
Comparison of Protein Active Site Structures for
... features of active sites by using descriptors mimicking important properties of amino acids. Instead, the CPASS database is composed of ligand-defined protein active site structures culled from the protein data bank (PDB). A total of 34,000 X-ray and NMR structures that are currently available in t ...
... features of active sites by using descriptors mimicking important properties of amino acids. Instead, the CPASS database is composed of ligand-defined protein active site structures culled from the protein data bank (PDB). A total of 34,000 X-ray and NMR structures that are currently available in t ...
Proteolytic Enzymes from Extremely Halophilic Bacteria
... for the extracellular enzyme. Furthermore, the cell-bound proteinase was completely inhibited by addition of 0.04 mg. poly-L-proline per ml., a compound which inhibits some proteolytic enzymes, (Sela & Katchalski 1959). No such inhibition was observed with the extracellular enzyme, and none of the e ...
... for the extracellular enzyme. Furthermore, the cell-bound proteinase was completely inhibited by addition of 0.04 mg. poly-L-proline per ml., a compound which inhibits some proteolytic enzymes, (Sela & Katchalski 1959). No such inhibition was observed with the extracellular enzyme, and none of the e ...
Inhibition of Protein Kinases by Balanol: Specificity within the Serine
... nearly two orders of magnitude greater than that for balanol itself. The effects of these derivatives on PKG are not identical with their effects on PKA: toward PKG, 140-decarboxylation and 100-dehydroxylation increase the Ki by about one order of magnitude, and removal of the seven-membered ring sh ...
... nearly two orders of magnitude greater than that for balanol itself. The effects of these derivatives on PKG are not identical with their effects on PKA: toward PKG, 140-decarboxylation and 100-dehydroxylation increase the Ki by about one order of magnitude, and removal of the seven-membered ring sh ...
Ribosome-tethered molecular chaperones
... associate specifically with ribosomes and bind to polypeptide chains that have just emerged from the tunnel. In addition, non-ribosome-bound chaperones act on longer nascent chains, either during the process of translation, or after they have been released from the ribosome. Although chaperones carr ...
... associate specifically with ribosomes and bind to polypeptide chains that have just emerged from the tunnel. In addition, non-ribosome-bound chaperones act on longer nascent chains, either during the process of translation, or after they have been released from the ribosome. Although chaperones carr ...
PDF + SI - GenScript
... complex exists, we embarked on independent purification methods. We prepared 0- to 12-h embryo extracts from Drosophila expressing a functional, FLAG-tagged version of Mcm6 (32). A single-step affinity purification of FLAG-Mcm6 from these flies captures mainly FLAG-Mcm6 in complex with Mcm2, Mcm4, a ...
... complex exists, we embarked on independent purification methods. We prepared 0- to 12-h embryo extracts from Drosophila expressing a functional, FLAG-tagged version of Mcm6 (32). A single-step affinity purification of FLAG-Mcm6 from these flies captures mainly FLAG-Mcm6 in complex with Mcm2, Mcm4, a ...
Endoplasmic Reticulum-Associated Degradation of Ricin A Chain
... Proteins that fail to fold in the endoplasmic reticulum (ER) or cannot find a pattern for assembly are often disposed of by a process named ER-associated degradation (ERAD), which involves transport of the substrate protein across the ER membrane (dislocation) followed by rapid proteasome-mediated p ...
... Proteins that fail to fold in the endoplasmic reticulum (ER) or cannot find a pattern for assembly are often disposed of by a process named ER-associated degradation (ERAD), which involves transport of the substrate protein across the ER membrane (dislocation) followed by rapid proteasome-mediated p ...
Enzymes - africangreyparrott.com
... A. This enzyme works best at a temp. of 35 C and a pH of 8. B. This enzyme works best at a temp. of 50 C and a pH of 12. C. Temp. and pH have no influence on the activity of this enzyme. D. This enzyme works best at a temp. above 50 C and a pH above ...
... A. This enzyme works best at a temp. of 35 C and a pH of 8. B. This enzyme works best at a temp. of 50 C and a pH of 12. C. Temp. and pH have no influence on the activity of this enzyme. D. This enzyme works best at a temp. above 50 C and a pH above ...
Plant serine/arginine-rich proteins and their role in pre
... one of these proteins (UBP) in protoplasts enhanced the splicing of pre-mRNAs that are otherwise inefficiently processed. The importance of the GC content in exons in efficient splicing, and an AG-rich exonic element in promoting downstream 5 0 splice site selection, has also been reported in some c ...
... one of these proteins (UBP) in protoplasts enhanced the splicing of pre-mRNAs that are otherwise inefficiently processed. The importance of the GC content in exons in efficient splicing, and an AG-rich exonic element in promoting downstream 5 0 splice site selection, has also been reported in some c ...
Protein Targeting to the Nuclear Pore. What Can
... biochemical pathways typical of the various types of organelles requires selective targeting apparatuses. Because each type of organelle contains its own targeting apparatus, proteins destined for a particular organelle must contain the proper targeting signal(s) for entry. These signaldependent tar ...
... biochemical pathways typical of the various types of organelles requires selective targeting apparatuses. Because each type of organelle contains its own targeting apparatus, proteins destined for a particular organelle must contain the proper targeting signal(s) for entry. These signaldependent tar ...
of Pseudomonas aeruginosa - BMC Microbiology
... Penicillin-Binding Proteins (PBPs) are acyl-serin transferases, initially identified by their ability to form covalent bonds with penicillin [10]. They are located on the external face of the inner membrane and share common D,D-peptidase activities (D,D-transpeptidase, D,D-carboxypeptidase or D,D-en ...
... Penicillin-Binding Proteins (PBPs) are acyl-serin transferases, initially identified by their ability to form covalent bonds with penicillin [10]. They are located on the external face of the inner membrane and share common D,D-peptidase activities (D,D-transpeptidase, D,D-carboxypeptidase or D,D-en ...
Electron microscopy in structural studies of Photosystem II
... 1. Projection contours of the particles associated with EFs, PFs and ESs planes obtained by analysis of EM images of 2-D particle arrays from freeze-fracture and freeze-etching experiments: (a) 2-D array of EFs particles from spinach (Simpson 1978). (b) 2-D array of EFs particles from Arabidopsis (T ...
... 1. Projection contours of the particles associated with EFs, PFs and ESs planes obtained by analysis of EM images of 2-D particle arrays from freeze-fracture and freeze-etching experiments: (a) 2-D array of EFs particles from spinach (Simpson 1978). (b) 2-D array of EFs particles from Arabidopsis (T ...
Protein dynamics and proteolysis in plant vacuoles
... cleaved from the precursor and it enters the biosynthetic pathway (also called the secretion pathway). Large segments of the precursor correspond to the mature vacuolar protein. Precursors can contain more than one subunit of the mature protein (e.g. 2S and 11S storage proteins; Müntz, 1998) or eve ...
... cleaved from the precursor and it enters the biosynthetic pathway (also called the secretion pathway). Large segments of the precursor correspond to the mature vacuolar protein. Precursors can contain more than one subunit of the mature protein (e.g. 2S and 11S storage proteins; Müntz, 1998) or eve ...
Detection of plum pox potyviral protein–protein interactions in planta
... which are important for the assembly of particles. However, neither the core nor the C-terminal region was determined to be necessary for the physical self-interaction of the CPs in SMV-G7H (Kang et al., 2006) and PPV. This may indicate that the aforementioned amino acid motifs are not essential for ...
... which are important for the assembly of particles. However, neither the core nor the C-terminal region was determined to be necessary for the physical self-interaction of the CPs in SMV-G7H (Kang et al., 2006) and PPV. This may indicate that the aforementioned amino acid motifs are not essential for ...
Structure-activity Relationships in Flexible Protein Domains
... physiological role of the interaction of RhoGDI with the GTP-bound form of a GTPase remains to be established. Overall, when exogenously introduced into cells the GDIs behave as negative regulators by maintaining rho GTPases as inactive cytosolic forms which are unable to effectively interact with G ...
... physiological role of the interaction of RhoGDI with the GTP-bound form of a GTPase remains to be established. Overall, when exogenously introduced into cells the GDIs behave as negative regulators by maintaining rho GTPases as inactive cytosolic forms which are unable to effectively interact with G ...
Protein conformational changes induced by adsorption onto material
... unfolded. The biological role of nonfolded protein domains is also the focus of considerable work in structural biology [25]. The classic view of Langmuir isotherm for protein adsorption on material surfaces assumes that some interaction energy is reversibly available to form a protein-material comp ...
... unfolded. The biological role of nonfolded protein domains is also the focus of considerable work in structural biology [25]. The classic view of Langmuir isotherm for protein adsorption on material surfaces assumes that some interaction energy is reversibly available to form a protein-material comp ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.