Design and application of stimulus
... peptides and thus trigger peptide aggregation (Table I). This has been achieved either through the design of ionic selfcomplementary peptides containing alternating hydrophobic and charged amino acids that assemble into macromolecular structures such as hydrogels and membranes upon salt exposure (Zh ...
... peptides and thus trigger peptide aggregation (Table I). This has been achieved either through the design of ionic selfcomplementary peptides containing alternating hydrophobic and charged amino acids that assemble into macromolecular structures such as hydrogels and membranes upon salt exposure (Zh ...
SPA Enzyme Assay Design
... protein). In the case of crude enzyme, competing activities may also be present in the cell lysate that could affect the assay performance. The use of specific substrates may overcome this issue, or it may be necessary to add inhibitors to block the competing activities. In the case of pure enzymes, ...
... protein). In the case of crude enzyme, competing activities may also be present in the cell lysate that could affect the assay performance. The use of specific substrates may overcome this issue, or it may be necessary to add inhibitors to block the competing activities. In the case of pure enzymes, ...
Article
... Another strategy for determining membrane topology involves tagging a protein with an enzyme or epitope. Tagging is often used in conjunction with other studies such as glycosylation or protease assays to give a more complete picture of membrane topology. In one study, the C‑terminal end of each of ...
... Another strategy for determining membrane topology involves tagging a protein with an enzyme or epitope. Tagging is often used in conjunction with other studies such as glycosylation or protease assays to give a more complete picture of membrane topology. In one study, the C‑terminal end of each of ...
as a PDF
... regulates the activity of these enzymes. Crystallographic data indicates that some RPTPs form dimers in which each monomer is precluded from binding substrate due to the insertion of a helix-turn-helix segment of the opposing monomer into the active site. I introduced “tagged” RPTP constructs into D ...
... regulates the activity of these enzymes. Crystallographic data indicates that some RPTPs form dimers in which each monomer is precluded from binding substrate due to the insertion of a helix-turn-helix segment of the opposing monomer into the active site. I introduced “tagged” RPTP constructs into D ...
pMAL FAQs
... structure for the protein. Some proteins, like MBP itself, can fold properly either in the cytoplasm or when exported to the periplasm. However, the normal folding pathway for some proteins is incompatible with passage through the membrane. In these cases, the fusion protein gets stuck in the membra ...
... structure for the protein. Some proteins, like MBP itself, can fold properly either in the cytoplasm or when exported to the periplasm. However, the normal folding pathway for some proteins is incompatible with passage through the membrane. In these cases, the fusion protein gets stuck in the membra ...
Systematic Analysis of Arabidopsis Organelles
... ordered assembly of more than one molecular function such as regulation of transcription. The cellular component term describes the macromolecular subcellular constituents of the cell such as the nucleus or the plasma membrane. With each category, terms are organized into a hierarchy where general t ...
... ordered assembly of more than one molecular function such as regulation of transcription. The cellular component term describes the macromolecular subcellular constituents of the cell such as the nucleus or the plasma membrane. With each category, terms are organized into a hierarchy where general t ...
Initiation of Innate Immune Responses in the
... from foreign "non-self" substances. During evolution, two general immune systems have developed to detect foreign substances namely innate (natural) immunity and adaptive (acquired) immunity. The innate immune system is phylogenetically a more ancient defense mechanism and can be found in all multic ...
... from foreign "non-self" substances. During evolution, two general immune systems have developed to detect foreign substances namely innate (natural) immunity and adaptive (acquired) immunity. The innate immune system is phylogenetically a more ancient defense mechanism and can be found in all multic ...
Engineering and Identifying Supercharged Proteins
... Most recently, we have demonstrated that these delivery properties are not unique to engineered cationic supercharged proteins but are instead present in a diverse class of naturally occurring supercharged human proteins (Cronican, 2011). We analyzed the human proteome for proteins with unusually hi ...
... Most recently, we have demonstrated that these delivery properties are not unique to engineered cationic supercharged proteins but are instead present in a diverse class of naturally occurring supercharged human proteins (Cronican, 2011). We analyzed the human proteome for proteins with unusually hi ...
Full-Text PDF
... cell homeostasis, and reproduction among others. Recently, the identification of an increasing number of secreted signal peptide-lacking proteins, also called leaderless secretory proteins, revealed the existence of unconventional protein secretion (UPS) pathways where these proteins bypass intermed ...
... cell homeostasis, and reproduction among others. Recently, the identification of an increasing number of secreted signal peptide-lacking proteins, also called leaderless secretory proteins, revealed the existence of unconventional protein secretion (UPS) pathways where these proteins bypass intermed ...
A Genomic Screen in Yeast Reveals Novel Aspects of
... his3-nonstop mutation: Nonstop mRNA decay is not an essential process in yeast (van Hoof et al. 2002). In addition, mutations that inactivate nonstop mRNA decay partially suppress a his3-nonstop allele. Specifically, van Hoof et al. (2002) showed that a wild-type strain containing a his3-nonstop all ...
... his3-nonstop mutation: Nonstop mRNA decay is not an essential process in yeast (van Hoof et al. 2002). In addition, mutations that inactivate nonstop mRNA decay partially suppress a his3-nonstop allele. Specifically, van Hoof et al. (2002) showed that a wild-type strain containing a his3-nonstop all ...
The Plant Cell - College of Computer, Mathematical, and Natural
... cells, the activity of V-ATPase is thought to be paramount to that of the V-PPase. Certainly, inorganic phosphate (PPi) is an important energy source for plant cells, and the V-PPase is a ubiquitous and active H1 pump (Rea and Poole, 1993; Zhen et al., 1997). Nevertheless, it is unclear how the two ...
... cells, the activity of V-ATPase is thought to be paramount to that of the V-PPase. Certainly, inorganic phosphate (PPi) is an important energy source for plant cells, and the V-PPase is a ubiquitous and active H1 pump (Rea and Poole, 1993; Zhen et al., 1997). Nevertheless, it is unclear how the two ...
Defining the tree of life: Archaea`s place in the evolutionary
... demonstrated that there exists a group of prokaryotic single celled organisms that, “appear to be no more related to the typical bacteria than they are to eukaryotic cytoplasm.” Based on comparisons of the 16S (18S in eukaryotes) ribosomal RNA of these organisms with that of bacteria and eukaryotes, ...
... demonstrated that there exists a group of prokaryotic single celled organisms that, “appear to be no more related to the typical bacteria than they are to eukaryotic cytoplasm.” Based on comparisons of the 16S (18S in eukaryotes) ribosomal RNA of these organisms with that of bacteria and eukaryotes, ...
9/12
... Lophotrichous = tuft at one or both ends Peritrichous = spread evenly over the entire surface Arrangement can be useful in identification ...
... Lophotrichous = tuft at one or both ends Peritrichous = spread evenly over the entire surface Arrangement can be useful in identification ...
Modeling studies of potato nucleoside triphosphate diphosphohydro
... The catalytic mechanism of ATP diphosphohydrolases is unclear. These enzymes, like potato apyrase (NTPDase1), hydrolyze ATP and ADP with similar velocities. The Ksh value, which is the ratio of ATP to ADP hydrolysis velocities, is between 1 and 0.5 (Komoszyński & Wojtczak, 1996; Kukulski & Komoszyńs ...
... The catalytic mechanism of ATP diphosphohydrolases is unclear. These enzymes, like potato apyrase (NTPDase1), hydrolyze ATP and ADP with similar velocities. The Ksh value, which is the ratio of ATP to ADP hydrolysis velocities, is between 1 and 0.5 (Komoszyński & Wojtczak, 1996; Kukulski & Komoszyńs ...
Identification and characterization of novel interaction partners of
... proapoptotic proteins Reaper Hid and Grim (RHG). Structurally DIAP1 has two BIRdomains, which mediate binding to caspases and also to RHG proteins. Additionally, DIAP1 contains a C-terminal RING E3 ubiquitin ligase domain. Although DIAP1 is a key anti-apoptotic protein in Drosophila, little is known ...
... proapoptotic proteins Reaper Hid and Grim (RHG). Structurally DIAP1 has two BIRdomains, which mediate binding to caspases and also to RHG proteins. Additionally, DIAP1 contains a C-terminal RING E3 ubiquitin ligase domain. Although DIAP1 is a key anti-apoptotic protein in Drosophila, little is known ...
thebacterialflagellum
... folded flagellin protein, except domain D2, are small enough to pass through the hollow tube through the hook and the filament. This suggests that the flagellin subunit is not completely folded when the export system inserts it into the central channel. This cavity seems to have the right size to al ...
... folded flagellin protein, except domain D2, are small enough to pass through the hollow tube through the hook and the filament. This suggests that the flagellin subunit is not completely folded when the export system inserts it into the central channel. This cavity seems to have the right size to al ...
Plant mitochondria contain the protein translocase subunits TatB
... The twin-arginine translocation (Tat) pathway differs from most other protein translocating systems in that it transports fully folded proteins (Lee et al., 2006). It is named after its targeting signal, which contains a pair of adjacent arginine residues (the twin arginines). Identified in all doma ...
... The twin-arginine translocation (Tat) pathway differs from most other protein translocating systems in that it transports fully folded proteins (Lee et al., 2006). It is named after its targeting signal, which contains a pair of adjacent arginine residues (the twin arginines). Identified in all doma ...
Somatic cancer mutations in the MLL1 histone methyltransferase
... interaction of the RA heterodimer with MLL1, it has been found that MLL1 requires all the three complex partners (WDR5, ASH2L, RBBP5) to exhibit the maximal methyl transferase activity (Cao et al., 2010; Li et al., 2016; Southall et al., 2009). This is in contrast to the MLL1 homologs MLL2, MLL3, an ...
... interaction of the RA heterodimer with MLL1, it has been found that MLL1 requires all the three complex partners (WDR5, ASH2L, RBBP5) to exhibit the maximal methyl transferase activity (Cao et al., 2010; Li et al., 2016; Southall et al., 2009). This is in contrast to the MLL1 homologs MLL2, MLL3, an ...
The RNA-binding protein repertoire of embryonic
... RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-link ...
... RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and yet annotation of RBPs is limited mainly to those with known RNA-binding domains. To systematically identify the RBPs of embryonic stem cells (ESCs), we here employ interactome capture, which combines UV cross-link ...
ARF1 and SAR1 GTPases in Endomembrane Trafficking in Plants
... mammalian system. It was also established in the same system that COPI proteins are involved in transport along the endocytic pathway [19,20]. During the selective transport of vesicles, the coat proteins must distinguish between cargo and resident proteins of the donor organelle. In intracellular t ...
... mammalian system. It was also established in the same system that COPI proteins are involved in transport along the endocytic pathway [19,20]. During the selective transport of vesicles, the coat proteins must distinguish between cargo and resident proteins of the donor organelle. In intracellular t ...
eIF-3 - Universidad Autónoma de San Luis Potosí
... initiation complex at the 5’ end. The poly(A)-binding protein (PAB1P in yeast) is required for this effect. Pab1p binds to eIF-4G, which in turn is bound to eIF4E. This implies that the mRNA must (transiently) have a circular organization, with both the 5’ and 3’ ends held in this complex. ...
... initiation complex at the 5’ end. The poly(A)-binding protein (PAB1P in yeast) is required for this effect. Pab1p binds to eIF-4G, which in turn is bound to eIF4E. This implies that the mRNA must (transiently) have a circular organization, with both the 5’ and 3’ ends held in this complex. ...
Genetically encoded phenyl azide photochemistry drives
... variant has an essentially identical structure to mCherry with both the chromophore and residues at 143 occupying similar positions and planes (Fig. 2c). The packing around the chromophore was also very similar (Fig. S3‡). Thus, inserting a phenyl azide moiety into the core of the protein did not si ...
... variant has an essentially identical structure to mCherry with both the chromophore and residues at 143 occupying similar positions and planes (Fig. 2c). The packing around the chromophore was also very similar (Fig. S3‡). Thus, inserting a phenyl azide moiety into the core of the protein did not si ...
Initial characterization of ayrRABC
... general secretory pathway requires its proteolytic activity to release proteins from their membrane-bound N-terminal leader sequences after translocation across the cytoplasmic membrane. Here, we identify the Staphylococcus aureus operon ayrRABC (SA0337 to SA0340) and show that once released from re ...
... general secretory pathway requires its proteolytic activity to release proteins from their membrane-bound N-terminal leader sequences after translocation across the cytoplasmic membrane. Here, we identify the Staphylococcus aureus operon ayrRABC (SA0337 to SA0340) and show that once released from re ...
Expression and purification of proteins using Strep
... The Twin-Strep-tag® and Strep-tag®II expression vectors pASK-IBA/pASG-IBA carry the promoter/operator region from the tetA resistance gene and are the state-of-the-art solution for such an inducible expression system [4, 5]. The strength of the tetA promoter is comparable with that of the lac-UV5 pr ...
... The Twin-Strep-tag® and Strep-tag®II expression vectors pASK-IBA/pASG-IBA carry the promoter/operator region from the tetA resistance gene and are the state-of-the-art solution for such an inducible expression system [4, 5]. The strength of the tetA promoter is comparable with that of the lac-UV5 pr ...
The structural basis of substrate translocation by the
... sequence, to another organic phosphate/inorganic phosphate antiporter, UhpT from E. coli [29,31], which carries out the electroneutral exchange of glucose-6-phosphate for Pi [32]. Extensive mutagenesis, biochemical and functional characterization has been carried out for UhpT, mostly by the Maloney ...
... sequence, to another organic phosphate/inorganic phosphate antiporter, UhpT from E. coli [29,31], which carries out the electroneutral exchange of glucose-6-phosphate for Pi [32]. Extensive mutagenesis, biochemical and functional characterization has been carried out for UhpT, mostly by the Maloney ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.