Phosphoproteomic analysis of Arabidopsis thaliana Hanna Klang Årstrand
... 3.2 Plant growth and protein preparation ......................................................................... 9 3.2.1 Isolation of ribosomes by ultracentrifugation ...................................................... 9 3.3 Proteomics and protein phosphorylation ............................... ...
... 3.2 Plant growth and protein preparation ......................................................................... 9 3.2.1 Isolation of ribosomes by ultracentrifugation ...................................................... 9 3.3 Proteomics and protein phosphorylation ............................... ...
intact-protein based sample preparation strategies for
... because albumin was concentrated within the membranes between pH 5.6 and 6.1, the acidic and basic chambers were both free of albumin; that method resulted in an increase in the number of highly acidic and basic proteins in the fractionated sample compared to whole plasma. Pedersen applied the same ...
... because albumin was concentrated within the membranes between pH 5.6 and 6.1, the acidic and basic chambers were both free of albumin; that method resulted in an increase in the number of highly acidic and basic proteins in the fractionated sample compared to whole plasma. Pedersen applied the same ...
The protein import apparatus of chloroplasts
... cross I ink product localized in the inner envelope membrane protein of 30 kDa. (The molecular mass data mentioned above are estimates after subtraction of the molecular mass of the cross linked precursor). (2) Upon precursor binding to spinach chloroplasts a specific increase in the ATP dependent p ...
... cross I ink product localized in the inner envelope membrane protein of 30 kDa. (The molecular mass data mentioned above are estimates after subtraction of the molecular mass of the cross linked precursor). (2) Upon precursor binding to spinach chloroplasts a specific increase in the ATP dependent p ...
Solubilization of Membrane Proteins into Functional Lipid‐Bilayer
... downshifted upon solubilization by moderate DIBMA concentrations (Figure 3 d). This suggests much less perturbation of lipid packing by DIBMA compared with SMA(3:1), the stronger effect of which is thought to result from intrusion of its phenyl rings into the bilayer core.[19, 20] With DIBMALPs, sim ...
... downshifted upon solubilization by moderate DIBMA concentrations (Figure 3 d). This suggests much less perturbation of lipid packing by DIBMA compared with SMA(3:1), the stronger effect of which is thought to result from intrusion of its phenyl rings into the bilayer core.[19, 20] With DIBMALPs, sim ...
Scavenging of 14-3-3 proteins reveals their involvement in the cell
... only affected the cell-surface transport of KATP channels when Arg-based signals are present in the channel complex. For these experiments, we used a SUR1-Kir6.2 fusion protein, in which the C-terminus of SUR1 was linked to the N-terminus of Kir6.2 (Fig. 3). This was necessary because mutation of th ...
... only affected the cell-surface transport of KATP channels when Arg-based signals are present in the channel complex. For these experiments, we used a SUR1-Kir6.2 fusion protein, in which the C-terminus of SUR1 was linked to the N-terminus of Kir6.2 (Fig. 3). This was necessary because mutation of th ...
MINI REVIEW Lectin-like proteins in model organisms: implications
... The lectins that contain CRDs listed in the Table I fall broadly in two categories. Lectins that contain CRDs in the first three structural groups are located mostly intracellularly, in luminal compartments. They function in the trafficking, sorting, and targetting of glycoproteins in the secretory ...
... The lectins that contain CRDs listed in the Table I fall broadly in two categories. Lectins that contain CRDs in the first three structural groups are located mostly intracellularly, in luminal compartments. They function in the trafficking, sorting, and targetting of glycoproteins in the secretory ...
Phagosome maturation in unicellular eukaryote Paramecium: the
... uring phagocytosis cells internalise large particles that are subsequently degraded in lysosomes that comprise the final stage of the endocytic pathways and intracellular traffic (Kornfeld and Mellman 1989; Gruenberg 2001; Luzio et al. 2003). Digestion capability of phagosomes is acquired in the pro ...
... uring phagocytosis cells internalise large particles that are subsequently degraded in lysosomes that comprise the final stage of the endocytic pathways and intracellular traffic (Kornfeld and Mellman 1989; Gruenberg 2001; Luzio et al. 2003). Digestion capability of phagosomes is acquired in the pro ...
An ADP/ATP-Specific Mitochondrial Carrier Protein
... import, but none of the other potential substrates produced more than a slight reduction of nucleotide transport (Fig. 5a), indicating a high specificity. ATP transport activity and inhibition of the A. locustae MCF protein To determine the kinetic properties of the A. locustae MCF protein, we analy ...
... import, but none of the other potential substrates produced more than a slight reduction of nucleotide transport (Fig. 5a), indicating a high specificity. ATP transport activity and inhibition of the A. locustae MCF protein To determine the kinetic properties of the A. locustae MCF protein, we analy ...
Protein methylation at the surface and buried deep
... are central to the recognition of trimethyllysine residues by a novel histone H3 binding module [40]. The 56 members of the SET-domain family encoded by the human genome can be divided into ten classes by their amino acid sequences [4]. Nine of these classes, with 53 members in total, were initially ...
... are central to the recognition of trimethyllysine residues by a novel histone H3 binding module [40]. The 56 members of the SET-domain family encoded by the human genome can be divided into ten classes by their amino acid sequences [4]. Nine of these classes, with 53 members in total, were initially ...
STRUCTURE AND ALLERGENICITY OF WHEAT GLUTEN PROTEINS
... A different structural model exists for the highest molecular weight gliadins. Omega-gliadins contain short, distinct C and N terminal sequences, separated by a region of repeatable sequences similar to γ gliadins. The sequence differs from that of γ gliadins, however, in having additional glutamine ...
... A different structural model exists for the highest molecular weight gliadins. Omega-gliadins contain short, distinct C and N terminal sequences, separated by a region of repeatable sequences similar to γ gliadins. The sequence differs from that of γ gliadins, however, in having additional glutamine ...
Intrinsic Disorder in Cell-signaling and Cancer
... (Figure 1). The same ranking was observed whether the results were presented as percentages of proteins (Figure 1(a)) or as percentages of residues (Figure 1(b)). The percentages of proteins (^ two standard errors) with 30 or more consecutive residues predicted to be disordered were 79(^ 5)% for HCA ...
... (Figure 1). The same ranking was observed whether the results were presented as percentages of proteins (Figure 1(a)) or as percentages of residues (Figure 1(b)). The percentages of proteins (^ two standard errors) with 30 or more consecutive residues predicted to be disordered were 79(^ 5)% for HCA ...
Small-angle scattering studies of intrinsically disordered proteins
... traditional Kratky representation, I(s)s2 vs s, which qualitatively report on the compactness of biomolecules in solution, directly account for the ensemble nature of disordered proteins. In order to fully exploit the structural and dynamic information encoded in SAS data, it is necessary to use re ...
... traditional Kratky representation, I(s)s2 vs s, which qualitatively report on the compactness of biomolecules in solution, directly account for the ensemble nature of disordered proteins. In order to fully exploit the structural and dynamic information encoded in SAS data, it is necessary to use re ...
Document
... host cells. Propulsion is thought to be driven by actin polymerization at the bacterial cell surface and moving bacteria leave in their wake a tail of actin filaments. Actin assembly by L. monocytogenes requires the bacterial surface protein ActA and protein components present in host cell cytoplasm ...
... host cells. Propulsion is thought to be driven by actin polymerization at the bacterial cell surface and moving bacteria leave in their wake a tail of actin filaments. Actin assembly by L. monocytogenes requires the bacterial surface protein ActA and protein components present in host cell cytoplasm ...
Enzymes
... molecule produced by living organisms that can speed up a specific chemical reaction without itself being destroyed or changed in any way. They are produced by the living organism and are usually present in only very small amounts in various cells. ...
... molecule produced by living organisms that can speed up a specific chemical reaction without itself being destroyed or changed in any way. They are produced by the living organism and are usually present in only very small amounts in various cells. ...
Nucleolar targeting of BN46/51 - Journal of Cell Science
... polypeptide of 263 amino acids with a predicted size of 30.1 kDa that co-migrated with the 51 kDa subunit of BN46/51 when expressed in yeast. JG4.1 encoded a polypeptide of 249 amino acids with a predicted size of 28.8 kDa that comigrated with the 46 kDa subunit of BN46/51. JG4.1 was identical to JG ...
... polypeptide of 263 amino acids with a predicted size of 30.1 kDa that co-migrated with the 51 kDa subunit of BN46/51 when expressed in yeast. JG4.1 encoded a polypeptide of 249 amino acids with a predicted size of 28.8 kDa that comigrated with the 46 kDa subunit of BN46/51. JG4.1 was identical to JG ...
The epidermal intermediate filament proteins of
... nematode C. elegans. Not only the 8 C. elegans IF proteins earlier reported (Dodemont et al., 1994), but also the additional 3 proteins, available through the completed genome project, have the long coil 1b version. Since no sequences are available for the echinoderms, the precise origin of the shor ...
... nematode C. elegans. Not only the 8 C. elegans IF proteins earlier reported (Dodemont et al., 1994), but also the additional 3 proteins, available through the completed genome project, have the long coil 1b version. Since no sequences are available for the echinoderms, the precise origin of the shor ...
Proteomic Strategies to Analyze Cell
... The use of Proteomic strategies in the discovery process is imperative since post-transcriptional modification can produce dramatic changes in protein levels and activity that are invisible to DNA arrays. The introduction of new and improved proteomics solutions with increased sensitivity, specifici ...
... The use of Proteomic strategies in the discovery process is imperative since post-transcriptional modification can produce dramatic changes in protein levels and activity that are invisible to DNA arrays. The introduction of new and improved proteomics solutions with increased sensitivity, specifici ...
Human Signaling Protein 14-3-3 Interacts With
... bleeding disorder Bernard-Soulier syndrome, in which mutations involving three subunits have been described.2,3 Each GPIb subunit contains one or more 24–amino acid tandem repeats rich in leucine that have been demonstrated to participate in protein-protein interactions in other cells.1 Tyrosine pho ...
... bleeding disorder Bernard-Soulier syndrome, in which mutations involving three subunits have been described.2,3 Each GPIb subunit contains one or more 24–amino acid tandem repeats rich in leucine that have been demonstrated to participate in protein-protein interactions in other cells.1 Tyrosine pho ...
binding domains demonstrated in a plant split
... 2006). In Arabidopsis, the heteromeric Toc core complex contains a b-barrel protein-conducting channel (atToc75) and two GTPases (atToc33 and atToc159). AtToc33 and atToc159 confer import specificity by the recognition and binding of the transit peptide and therefore represent the import receptors a ...
... 2006). In Arabidopsis, the heteromeric Toc core complex contains a b-barrel protein-conducting channel (atToc75) and two GTPases (atToc33 and atToc159). AtToc33 and atToc159 confer import specificity by the recognition and binding of the transit peptide and therefore represent the import receptors a ...
PROTEIN SYNTHESIS: TRANSLATION AND
... subunits, each of which contains RNA and many proteins. With one exception, each protein is present in a single copy per ribosome, as is each RNA species. The composition of major ribosome types is shown in Table 17.1, and characteristics of their RNAs are given in Table 16.1. Ribosome architecture ...
... subunits, each of which contains RNA and many proteins. With one exception, each protein is present in a single copy per ribosome, as is each RNA species. The composition of major ribosome types is shown in Table 17.1, and characteristics of their RNAs are given in Table 16.1. Ribosome architecture ...
Chapter 8
... • The class 1 release factors respond to specific termination codons and hydrolyze the polypeptide-tRNA linkage. • The class 1 release factors are assisted by class 2 release factors (such as RF3) that depend on GTP. • The mechanism is similar in: – bacteria (which have two types of class 1 release ...
... • The class 1 release factors respond to specific termination codons and hydrolyze the polypeptide-tRNA linkage. • The class 1 release factors are assisted by class 2 release factors (such as RF3) that depend on GTP. • The mechanism is similar in: – bacteria (which have two types of class 1 release ...
MagneHis™ Protein Purification System Technical Manual
... for long term or at 4°C for up to one week. 7. Incubate with shaking for 10–20 minutes at room temperature on a rotary mixer or shaking platform. Method 2: Direct Lysis of Bacterial Cell Cultures See Section 3.A for preparation of cells before starting the experiment. 1. Add 110µl of FastBreak™ Cell ...
... for long term or at 4°C for up to one week. 7. Incubate with shaking for 10–20 minutes at room temperature on a rotary mixer or shaking platform. Method 2: Direct Lysis of Bacterial Cell Cultures See Section 3.A for preparation of cells before starting the experiment. 1. Add 110µl of FastBreak™ Cell ...
Characterization of proteins secreted from a Type III secretion
... and eseD genes in the wild-type strain as described growth of bacteria in the macrophages (Okuda et al. previously (Tan et al. 2005) with a slight modification 2006). However, only scant information is available on using the suicide vector plasmid, pRE112 (Okuda et al. the pathogenicity of E. tarda. ...
... and eseD genes in the wild-type strain as described growth of bacteria in the macrophages (Okuda et al. previously (Tan et al. 2005) with a slight modification 2006). However, only scant information is available on using the suicide vector plasmid, pRE112 (Okuda et al. the pathogenicity of E. tarda. ...
Membrane Transport - Austin Publishing Group
... they transport, whether they are always open or gated (open only when stimulated), and most importantly, whether a source of free energy is required for operation. ...
... they transport, whether they are always open or gated (open only when stimulated), and most importantly, whether a source of free energy is required for operation. ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.