A Late Mitotic Regulatory Network Controlling
... et al., 1998; Shirayama et al., 1998). Little is known about how these various regulatory influences are integrated to provide the correct timing of cyclin destruction. To ensure the proper order of mitotic events, the APC may also be regulated at the level of substrate specificity. APC-dependent ub ...
... et al., 1998; Shirayama et al., 1998). Little is known about how these various regulatory influences are integrated to provide the correct timing of cyclin destruction. To ensure the proper order of mitotic events, the APC may also be regulated at the level of substrate specificity. APC-dependent ub ...
Isolation by Calcium-Dependent Translocation to
... membrane. These proteins were of molecular mass approximately 66, 40, 39, and 35 kD and they have been shown earlier to correspond to annexins.'" Binding to these proteins was to be expected because the cytosol antiserum contains antibodies against annexins I, 11, IV, and VI, as shown by ELISA using ...
... membrane. These proteins were of molecular mass approximately 66, 40, 39, and 35 kD and they have been shown earlier to correspond to annexins.'" Binding to these proteins was to be expected because the cytosol antiserum contains antibodies against annexins I, 11, IV, and VI, as shown by ELISA using ...
Full Text - Molecular Biology and Evolution
... F(2,61)¼ 21.07; P ¼ 1.1107; x2 ¼ 0.149). Filtering for proteins shared by eukaryotes and at least one of the prokaryotic domains produces the results in figure 3B. While prokaryotes are largely unaltered and the difference between unicellular and multicellular eukaryotes remains, the effect become ...
... F(2,61)¼ 21.07; P ¼ 1.1107; x2 ¼ 0.149). Filtering for proteins shared by eukaryotes and at least one of the prokaryotic domains produces the results in figure 3B. While prokaryotes are largely unaltered and the difference between unicellular and multicellular eukaryotes remains, the effect become ...
Biochemical Origins of Alzheimer`s Disease With Treatment
... A production; developers, however, hit a roadblock when complications arose due to the fact that -secretase is also being responsible for cleaving other membrane proteins vital to cellular survival. LY-450139, the first -secretase inhibitor to go through clinical trials was found to decrease plas ...
... A production; developers, however, hit a roadblock when complications arose due to the fact that -secretase is also being responsible for cleaving other membrane proteins vital to cellular survival. LY-450139, the first -secretase inhibitor to go through clinical trials was found to decrease plas ...
Plant mitochondria contain the protein translocase subunits TatB
... that the At5g43680 and related proteins grouped most closely with the TatB proteins from bacteria indicating that At5g43680 is a possible TatB like protein (Figure 2). Although some of the bootstrap values are low we believe the tree is accurate. We also tested Bayesian homology using MrBayes (Ronq ...
... that the At5g43680 and related proteins grouped most closely with the TatB proteins from bacteria indicating that At5g43680 is a possible TatB like protein (Figure 2). Although some of the bootstrap values are low we believe the tree is accurate. We also tested Bayesian homology using MrBayes (Ronq ...
Supplementary information
... are presented in figure Sup. 1. It is clear that not only the nuclear mobility of any p53 variant is lower than this of ECFP-EYFP but also that the kinetics of fluorescence recovery is different. While p53 recovery curves are best described by the two phase exponential association model (with the ex ...
... are presented in figure Sup. 1. It is clear that not only the nuclear mobility of any p53 variant is lower than this of ECFP-EYFP but also that the kinetics of fluorescence recovery is different. While p53 recovery curves are best described by the two phase exponential association model (with the ex ...
Antigenic Properties of Mycoplasma Organisms and Membranes
... Agglutination of whole cell. This was carried out either on glass slides at room temperature or in test-tubes at 52O according to Bailey et al. (1961). Double difliion in agar. The test was done according to Ouchterlony (1964). Noble agar 1% (w/v) was prepared in veronal+HCl buffer (PH = 8.2; ionic ...
... Agglutination of whole cell. This was carried out either on glass slides at room temperature or in test-tubes at 52O according to Bailey et al. (1961). Double difliion in agar. The test was done according to Ouchterlony (1964). Noble agar 1% (w/v) was prepared in veronal+HCl buffer (PH = 8.2; ionic ...
Chp 19
... folding into native conformation §Proteolytic cleavage – common regulatory mechanism §Remove N-terminal methionine and signal peptides §Convert proproteins into their active forms §Preproprotein – inactive precursor with removable signal peptide §Preproinsulin – proinsulin - insulin ...
... folding into native conformation §Proteolytic cleavage – common regulatory mechanism §Remove N-terminal methionine and signal peptides §Convert proproteins into their active forms §Preproprotein – inactive precursor with removable signal peptide §Preproinsulin – proinsulin - insulin ...
Characterizing the O-glycosylation landscape of human plasma
... general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner. Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans w ...
... general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner. Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans w ...
- BioTek Instruments
... The fastest, simplest method to quantify proteins is via ultraviolet (UV) absorbance measurement at 280 nm (A280), whereby light absorbed by the protein directly correlates to protein concentration in accordance with the Beer-Lambert Law. Most proteins have a peak absorbance at this wavelength due t ...
... The fastest, simplest method to quantify proteins is via ultraviolet (UV) absorbance measurement at 280 nm (A280), whereby light absorbed by the protein directly correlates to protein concentration in accordance with the Beer-Lambert Law. Most proteins have a peak absorbance at this wavelength due t ...
The proteome of the infectious bronchitis virus Beau
... the spike (S), envelope (E), membrane (M) and nucleocapsid (N). S, M and E are all found integral to the virion membrane, while N complexes with the RNA genome, packaged inside the virion. The 15 non-structural proteins (nsps) are expressed as two replicase polyproteins that are post-translationally ...
... the spike (S), envelope (E), membrane (M) and nucleocapsid (N). S, M and E are all found integral to the virion membrane, while N complexes with the RNA genome, packaged inside the virion. The 15 non-structural proteins (nsps) are expressed as two replicase polyproteins that are post-translationally ...
Proteomic capacity of recent fluorescent dyes for
... compared from the patterns obtained with different quantities of a total extract of Arabidopsis proteins. In terms of sensitivity, assuming that the whole protein pattern was detected for 4 lg proteins (Fig. 1), SR appeared to be able to evidence a complex pattern down to ca. 0.5 lg, whereas the two ...
... compared from the patterns obtained with different quantities of a total extract of Arabidopsis proteins. In terms of sensitivity, assuming that the whole protein pattern was detected for 4 lg proteins (Fig. 1), SR appeared to be able to evidence a complex pattern down to ca. 0.5 lg, whereas the two ...
The Human Arp2/3 Complex Is Composed of Evolutionarily
... the control of actin assembly in cells, we determined the predicted amino acid sequences of all of the subunits of the human protein complex. Using peptide sequence information (Welch et al., 1997) to search the EMBL/GenBank/DDBJ dbEST we obtained and sequenced full-length human cDNA clones encoding ...
... the control of actin assembly in cells, we determined the predicted amino acid sequences of all of the subunits of the human protein complex. Using peptide sequence information (Welch et al., 1997) to search the EMBL/GenBank/DDBJ dbEST we obtained and sequenced full-length human cDNA clones encoding ...
Ro52: Structure and interactions of constructs of RING and B-box
... The ubiquitination process is vital to maintain the protein homeostasis in the cell. With high specificity it regulates degradation of proteins by tagging them with a small protein called ubiquitin. Four proteins are involved to perform the process and in this thesis one of these proteins is studied ...
... The ubiquitination process is vital to maintain the protein homeostasis in the cell. With high specificity it regulates degradation of proteins by tagging them with a small protein called ubiquitin. Four proteins are involved to perform the process and in this thesis one of these proteins is studied ...
mic.sgmjournals.org
... for the adaptation of Y. pestis to its in vivo life stages. Thirteen proteins that appear to be part of an intact type VI secretion system (T6SS) were identified in membrane fractions of stationaryphase cells grown at 26 6C, but not at 37 6C. The corresponding genes are clustered in the Y. pestis KI ...
... for the adaptation of Y. pestis to its in vivo life stages. Thirteen proteins that appear to be part of an intact type VI secretion system (T6SS) were identified in membrane fractions of stationaryphase cells grown at 26 6C, but not at 37 6C. The corresponding genes are clustered in the Y. pestis KI ...
Peptide inhibitors of the essential cell division protein FtsA
... new targets because they encode essential proteins leading to a lethal phenotype when inhibited. These proteins are highly conserved in bacterial species but absent in eukaryotic cells. They are extremely sensitive to inhibition because the division process depends on recruitment of specific protein ...
... new targets because they encode essential proteins leading to a lethal phenotype when inhibited. These proteins are highly conserved in bacterial species but absent in eukaryotic cells. They are extremely sensitive to inhibition because the division process depends on recruitment of specific protein ...
What Does the Microsporidian E. cuniculi Tell Us About the Origin of
... approaching the size of a minimal eukaryotic cell. To understand the differences between minimal cells of Eukarya and Bacteria, we need to characterize the set of proteins in E. cuniculi that is unique to the eukaryotes but absent from other cellular domains. Previously, we collected a set of protein ...
... approaching the size of a minimal eukaryotic cell. To understand the differences between minimal cells of Eukarya and Bacteria, we need to characterize the set of proteins in E. cuniculi that is unique to the eukaryotes but absent from other cellular domains. Previously, we collected a set of protein ...
669 Salmonella typhimurium - Journal of General Virology
... based on the fact that when unattached tail protein is bound to ceils, it is not found free in the medium until some time has passed, the extent of which depends on the multiplicity of tails added (Israel et al. ~972). The matter is perhaps best put in terms of probability. After each cleavage a tai ...
... based on the fact that when unattached tail protein is bound to ceils, it is not found free in the medium until some time has passed, the extent of which depends on the multiplicity of tails added (Israel et al. ~972). The matter is perhaps best put in terms of probability. After each cleavage a tai ...
enzymes - UniMAP Portal
... • In the 19th century, when studying the fermentation of sugar to alcohol by yeast, Louis Pasteur came to the conclusion that this fermentation was catalyzed by a vital force contained within the yeast cells called "ferments", which were thought to function only within living organisms. • He wrote t ...
... • In the 19th century, when studying the fermentation of sugar to alcohol by yeast, Louis Pasteur came to the conclusion that this fermentation was catalyzed by a vital force contained within the yeast cells called "ferments", which were thought to function only within living organisms. • He wrote t ...
Plant mitochondria contain the protein translocase subunits TatB
... that the At5g43680 and related proteins grouped most closely with the TatB proteins from bacteria indicating that At5g43680 is a possible TatB like protein (Figure 2). Although some of the bootstrap values are low we believe the tree is accurate. We also tested Bayesian homology using MrBayes (Ronq ...
... that the At5g43680 and related proteins grouped most closely with the TatB proteins from bacteria indicating that At5g43680 is a possible TatB like protein (Figure 2). Although some of the bootstrap values are low we believe the tree is accurate. We also tested Bayesian homology using MrBayes (Ronq ...
The YidC/Oxa1/Alb3 protein family Saller, Manfred J.
... Biological membranes of living cells form hydrophobic barriers that separate the intracellular space from the extracellular environment and allow the maintenance of distinct cellular compartments. Many essential cellular processes depend on the function of membrane proteins that are anchored to the ...
... Biological membranes of living cells form hydrophobic barriers that separate the intracellular space from the extracellular environment and allow the maintenance of distinct cellular compartments. Many essential cellular processes depend on the function of membrane proteins that are anchored to the ...
Part 2
... shown. Next, the downward arrows must appear along with for protein separation. These separated text followed by the blue bands on the left and the spots on proteins on the gel are used for further ...
... shown. Next, the downward arrows must appear along with for protein separation. These separated text followed by the blue bands on the left and the spots on proteins on the gel are used for further ...
Mitochondrial protein import: from transport pathways to an
... Small TIM chaperone complexes guide the hydrophobic precursor proteins to the TIM22 complex that mediates Dc-driven membrane insertion. Additionally, Tim54 promotes assembly of the i-AAA protease, linking the translocase to protein quality control. Recently discovered import components are marked in ...
... Small TIM chaperone complexes guide the hydrophobic precursor proteins to the TIM22 complex that mediates Dc-driven membrane insertion. Additionally, Tim54 promotes assembly of the i-AAA protease, linking the translocase to protein quality control. Recently discovered import components are marked in ...
ENZYMES
... • Enzymes are globular proteins. They have a complex tertiary and quaternary structure in which polypeptides are folded around each other to form a roughly spherical or globular shape. The overall 3D shape of an enzyme molecule is very important: if it is altered, the enzyme cannot bind to its subst ...
... • Enzymes are globular proteins. They have a complex tertiary and quaternary structure in which polypeptides are folded around each other to form a roughly spherical or globular shape. The overall 3D shape of an enzyme molecule is very important: if it is altered, the enzyme cannot bind to its subst ...
the unfolded protein response in yeast and mammals Chris
... An as-yet undiscovered mammalian Ire1 substrate mRNA may encode a transcription factor analogous to Hac1p in yeast, but it is also conceivable that Hac1p’s position in the pathway as proximal activator of transcription is filled by another protein whose activation is not regulated by splicing. Mamma ...
... An as-yet undiscovered mammalian Ire1 substrate mRNA may encode a transcription factor analogous to Hac1p in yeast, but it is also conceivable that Hac1p’s position in the pathway as proximal activator of transcription is filled by another protein whose activation is not regulated by splicing. Mamma ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.