y. Cell Set. Suppl. ¡1, 1-11 (1989) Printed in
... Mitochondria contain their own genetic system which manufactures most of the mitochondrial RNAs and a few (13 in humans) of the mitochondrial proteins. All the other hundreds of mitochondrial proteins, and probably also several mitochondrial RNAs, are encoded by nuclear genes and imported into the m ...
... Mitochondria contain their own genetic system which manufactures most of the mitochondrial RNAs and a few (13 in humans) of the mitochondrial proteins. All the other hundreds of mitochondrial proteins, and probably also several mitochondrial RNAs, are encoded by nuclear genes and imported into the m ...
Interaction of Graphene Oxide with Proteins and
... The enriched reactive oxygen functional groups of GO should render it a good solid substrate for protein immobilization through covalent binding. Covalent binding of a protein with GO is most commonly based on chemical reactions between the side groups of amino acid residues located on the protein s ...
... The enriched reactive oxygen functional groups of GO should render it a good solid substrate for protein immobilization through covalent binding. Covalent binding of a protein with GO is most commonly based on chemical reactions between the side groups of amino acid residues located on the protein s ...
Plant–pathogen interactions: what is proteomics telling us?
... Plant–bacterium interactions Bacteria rely on diverse secretion pathways in order to overcome plant defences and to establish successful colonization of the host plant. Five secretion systems (types I–V) have been reported in bacteria, which are distinguished by their constituent proteins [14]. The ...
... Plant–bacterium interactions Bacteria rely on diverse secretion pathways in order to overcome plant defences and to establish successful colonization of the host plant. Five secretion systems (types I–V) have been reported in bacteria, which are distinguished by their constituent proteins [14]. The ...
CHAPTER FOUR Pseudomonas aeruginosa INFLUENCED PLANKTONIC AND BIOFILM POPULATIONS BASED UPON
... protein spots in the pH range of 3.0 - 10.0 after silver staining. However, due to their hydrophobic nature, many bacterial outer membrane proteins (OMPs), membrane and cytoplasmic membrane proteins are missing from 2-DE gel proteome maps, thus hindering the analysis of complete proteomes (Molloy et ...
... protein spots in the pH range of 3.0 - 10.0 after silver staining. However, due to their hydrophobic nature, many bacterial outer membrane proteins (OMPs), membrane and cytoplasmic membrane proteins are missing from 2-DE gel proteome maps, thus hindering the analysis of complete proteomes (Molloy et ...
Coarse-Grained Modeling of ProteinDynamics
... and tested computationally on a very general level. This is the case of the studies of chaperonin action. Chaperonin and its protein substrate is a very large protein complex whose dynamic processes are way beyond the reach of classical dynamics simulation models. Over the past 20 years a significan ...
... and tested computationally on a very general level. This is the case of the studies of chaperonin action. Chaperonin and its protein substrate is a very large protein complex whose dynamic processes are way beyond the reach of classical dynamics simulation models. Over the past 20 years a significan ...
Targeting to the T. gondii plastid
... is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis ( ...
... is mediated by an N-terminal bipartite targeting sequence composed of an ER signal sequence followed by a chloroplast transit peptide-like domain (Schwartzbach et al., 1998). Like the chloroplasts of diatoms and euglenoids, the T. gondii apicoplast appears to have arisen by secondary endosymbiosis ( ...
Career of Tom Muir
... ■ Photolysis of protein in a cell ■ Dosable manner with low-intensity UV light ■ Valuable if different protein concentrations trigger different responses ■ Photolysis of small molecule can change function of protein ...
... ■ Photolysis of protein in a cell ■ Dosable manner with low-intensity UV light ■ Valuable if different protein concentrations trigger different responses ■ Photolysis of small molecule can change function of protein ...
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in
... Formation of an Hsp90Ec–DnaK–client protein complex is facilitated by CbpA We previously showed that Hsp90Ec functions synergistically with DnaK and its cochaperones, a J-domain protein (CbpA or DnaJ) and GrpE in client protein reactivation in vitro [26]. In addition, we showed that Hsp90Ec and DnaK ...
... Formation of an Hsp90Ec–DnaK–client protein complex is facilitated by CbpA We previously showed that Hsp90Ec functions synergistically with DnaK and its cochaperones, a J-domain protein (CbpA or DnaJ) and GrpE in client protein reactivation in vitro [26]. In addition, we showed that Hsp90Ec and DnaK ...
NUCLEAR PROTEINS II. Similarity of Nonhistone Proteins in
... resis technique, Comings and Tack (13) showed that in mouse liver nuclei the nuclear sap and n o n h i s t o n e proteins exhibited many similar bands, suggesting that they were not distinct entities. T h e nuclei a p p e a r e d to possess several classes of nonhistone proteins, some of which were ...
... resis technique, Comings and Tack (13) showed that in mouse liver nuclei the nuclear sap and n o n h i s t o n e proteins exhibited many similar bands, suggesting that they were not distinct entities. T h e nuclei a p p e a r e d to possess several classes of nonhistone proteins, some of which were ...
Proteomic Analysis of the Arabidopsis Nucleolus Suggests Novel
... component proteins and other macromolecules, their interactions, and their responses to changes in cellular activity. The availability of complete genome sequences, together with rapid advances in mass spectrometry methods for analyzing complex polypeptide mixtures, means that it is now possible to ...
... component proteins and other macromolecules, their interactions, and their responses to changes in cellular activity. The availability of complete genome sequences, together with rapid advances in mass spectrometry methods for analyzing complex polypeptide mixtures, means that it is now possible to ...
Biochem-EnzymesL
... Induced Fit Theory The enzyme does not actually form a chemical bond with the substrate. After the reaction, the products are released and the enzyme returns to its normal shape. Because the enzyme does not form chemical bonds with the substrate, it remains unchanged. The enzyme molecule can be reu ...
... Induced Fit Theory The enzyme does not actually form a chemical bond with the substrate. After the reaction, the products are released and the enzyme returns to its normal shape. Because the enzyme does not form chemical bonds with the substrate, it remains unchanged. The enzyme molecule can be reu ...
Discovery of Enzymes
... Induced Fit Theory The enzyme does not actually form a chemical bond with the substrate. After the reaction, the products are released and the enzyme returns to its normal shape. Because the enzyme does not form chemical bonds with the substrate, it remains unchanged. The enzyme molecule can be reu ...
... Induced Fit Theory The enzyme does not actually form a chemical bond with the substrate. After the reaction, the products are released and the enzyme returns to its normal shape. Because the enzyme does not form chemical bonds with the substrate, it remains unchanged. The enzyme molecule can be reu ...
Coca Cola
... - The actin and myosin filaments remain the same length - The sarcomere length shortens because the actin and myosin filaments slide relative each other animation 16.8- myosin (compare with picture 43) ...
... - The actin and myosin filaments remain the same length - The sarcomere length shortens because the actin and myosin filaments slide relative each other animation 16.8- myosin (compare with picture 43) ...
Table S2
... Table S2, continued Pds1: Inhibits the onset of anaphase by binding and sequestering the Esp1 protease that cleaves the cohesin complexes that hold sister chromatids together. Binding of Pds1 to Esp1 was reported to depend in Cdc28 phosphorylation[53] Sic1: Inhibitor of Clb-Cdc28. Phosphorylation o ...
... Table S2, continued Pds1: Inhibits the onset of anaphase by binding and sequestering the Esp1 protease that cleaves the cohesin complexes that hold sister chromatids together. Binding of Pds1 to Esp1 was reported to depend in Cdc28 phosphorylation[53] Sic1: Inhibitor of Clb-Cdc28. Phosphorylation o ...
Functional Analysis of Subunit e of the F1Fo
... CN-PAGE analysis of F1Fo-ATP synthase complexes. Mitochondria (200 g of protein) were lysed with 36 l of digitonin buffer (34 mM potassium acetate, 34 mM HEPES-KOH [pH 7.4], 11.4% glycerol, 1 mM phenylmethylsulfonyl fluoride) and various concentrations of digitonin (as indicated) for 30 min on ice ...
... CN-PAGE analysis of F1Fo-ATP synthase complexes. Mitochondria (200 g of protein) were lysed with 36 l of digitonin buffer (34 mM potassium acetate, 34 mM HEPES-KOH [pH 7.4], 11.4% glycerol, 1 mM phenylmethylsulfonyl fluoride) and various concentrations of digitonin (as indicated) for 30 min on ice ...
Analysis of protein carbonylation
... Determination of physiological concentrations, preferably circulating levels, of the oxidized proteins or their ...
... Determination of physiological concentrations, preferably circulating levels, of the oxidized proteins or their ...
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
... found in the periplasm, but the physiological functions of only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the functional expression of a wide variety of recombinant proteins from different sources. However, the periplasmi ...
... found in the periplasm, but the physiological functions of only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the functional expression of a wide variety of recombinant proteins from different sources. However, the periplasmi ...
Functional interaction between a novel protein phosphatase 2A
... 1996). One gene that gives rise to two dierentially spliced mRNAs encodes two B-subunits, PR72 (also called B'') and PR130 (Hendrix et al., 1993a). It is widely believed that the multitude of B subunits plays a role in regulation of phosphatase activity, control of substrate speci®city and targetin ...
... 1996). One gene that gives rise to two dierentially spliced mRNAs encodes two B-subunits, PR72 (also called B'') and PR130 (Hendrix et al., 1993a). It is widely believed that the multitude of B subunits plays a role in regulation of phosphatase activity, control of substrate speci®city and targetin ...
Molecular Components of the Bacterial Cytoskeleton
... protein polymer formation). The core structures of α- and β-tubulin are composed of two β-sheets surrounded by α-helices (Nogales et al. 1998b), making up two functional domains. The N-terminal of the two domains has a Rossmann fold similar to that of many ATPases, and it contains a GTP binding site ...
... protein polymer formation). The core structures of α- and β-tubulin are composed of two β-sheets surrounded by α-helices (Nogales et al. 1998b), making up two functional domains. The N-terminal of the two domains has a Rossmann fold similar to that of many ATPases, and it contains a GTP binding site ...
Adaptation and Protein Quality Control Under Metalloid
... Toxic metals and metalloids are emerging as major environmental pollutants, having ecological consequences as well as being linked to a broad range of degenerative conditions in animals, plants and humans. While the toxicity of several metalloids is well established, the underlying molecular mechani ...
... Toxic metals and metalloids are emerging as major environmental pollutants, having ecological consequences as well as being linked to a broad range of degenerative conditions in animals, plants and humans. While the toxicity of several metalloids is well established, the underlying molecular mechani ...
New insights into regulation of p53 protein degradation
... and that it prevents the degradation of proteins with unstructured regions, such as p53, p73 [33, 41], leads to stabilization of p53 and cellular protection [36]. Protein-protein interactions can protect p53 protein against ubiquitin-independent 20S proteasome pathway action The second proposed mech ...
... and that it prevents the degradation of proteins with unstructured regions, such as p53, p73 [33, 41], leads to stabilization of p53 and cellular protection [36]. Protein-protein interactions can protect p53 protein against ubiquitin-independent 20S proteasome pathway action The second proposed mech ...
A protein domain interaction interface database: InterPare | BMC
... the website. As the threshold gets higher the number of interface residue gets smaller. We used SCOP 1.65 as a domain definition. It contains 54,745 domains from 20,619 PDB Entries (August 2003). InterPare, at the time of this writing, contains 26,999 PDB entries (September 2004). At present, there ...
... the website. As the threshold gets higher the number of interface residue gets smaller. We used SCOP 1.65 as a domain definition. It contains 54,745 domains from 20,619 PDB Entries (August 2003). InterPare, at the time of this writing, contains 26,999 PDB entries (September 2004). At present, there ...
Ion homeostasis, channels, and transporters: an update on cellular
... transport protein vary depending on the cell type in which it is expressed? ...
... transport protein vary depending on the cell type in which it is expressed? ...
Acetylcholine Receptor-associated 43K Protein Contains Covalently
... protease was added and the incubation was continued for an additional 10 h. Gel fragments were removed by centrifugation for 15 min in a microfuge and the supernatant, containing digested 43K protein, was supplemented with 20 I.tl of a 20 mg/ml stock of methyl myristate (Sigma Chemical Co.) in metha ...
... protease was added and the incubation was continued for an additional 10 h. Gel fragments were removed by centrifugation for 15 min in a microfuge and the supernatant, containing digested 43K protein, was supplemented with 20 I.tl of a 20 mg/ml stock of methyl myristate (Sigma Chemical Co.) in metha ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.