SpyTag and SpyCatcher - Department of Biochemistry
... [22,23]. We generated SpyTag and SpyCatcher by splitting the CnaB2 domain from the fibronectin-binding protein FbaB from Streptococcus pyogenes (Spy) [24,25]. CnaB2 was split into a 13 residue peptide (SpyTag) and the 116 residue complementary domain (SpyCatcher) (Figure 1a). These two parts spontan ...
... [22,23]. We generated SpyTag and SpyCatcher by splitting the CnaB2 domain from the fibronectin-binding protein FbaB from Streptococcus pyogenes (Spy) [24,25]. CnaB2 was split into a 13 residue peptide (SpyTag) and the 116 residue complementary domain (SpyCatcher) (Figure 1a). These two parts spontan ...
Enzyme Substrate Interactions Identification of Enzyme Catalytic Site
... • The favored model of enzyme substrate interaction is known as the induced fit model. • This model proposes that the initial interaction between enzyme and substrate is relatively weak, – These weak interactions rapidly induce conformational changes in the enzyme that strengthen binding and bring c ...
... • The favored model of enzyme substrate interaction is known as the induced fit model. • This model proposes that the initial interaction between enzyme and substrate is relatively weak, – These weak interactions rapidly induce conformational changes in the enzyme that strengthen binding and bring c ...
cytoplasm nucleus and the A specific subset of
... 1997). These include nucleolar proteins, such as NO38, nucleolin, and Nopp140 (Borer et al. 1989; Meier and Blobel 1992), steroid hormone receptors, such as the progesterone receptor (Guiochon-Mantel et al. 1991), the U1 snRNP-specific polypeptide U1A (Kambach and Mattaj 1992), heat shock-related pr ...
... 1997). These include nucleolar proteins, such as NO38, nucleolin, and Nopp140 (Borer et al. 1989; Meier and Blobel 1992), steroid hormone receptors, such as the progesterone receptor (Guiochon-Mantel et al. 1991), the U1 snRNP-specific polypeptide U1A (Kambach and Mattaj 1992), heat shock-related pr ...
Selenium incorporation using recombinant techniques
... Hendrickson first reported the possibility of using selenomethionine (SeMet) as a phasing tool in 1990 and the ease of incorporating selenium in recombinant systems has led to selenium being the first choice for multiple-wavelength (and single-wavelength) anomalous dispersion experiments (Figs. 1a a ...
... Hendrickson first reported the possibility of using selenomethionine (SeMet) as a phasing tool in 1990 and the ease of incorporating selenium in recombinant systems has led to selenium being the first choice for multiple-wavelength (and single-wavelength) anomalous dispersion experiments (Figs. 1a a ...
Purification and Characterization of the Secondary
... assimilated through the glyoxylate cycle. The C, moiety is assimilated through the cellular reduced C , pool (Coleman & Perry, 1984). Little is known about the enzymes responsible for the series of reactions that convert propane to acetate in this organism. There have been reports on the intermediar ...
... assimilated through the glyoxylate cycle. The C, moiety is assimilated through the cellular reduced C , pool (Coleman & Perry, 1984). Little is known about the enzymes responsible for the series of reactions that convert propane to acetate in this organism. There have been reports on the intermediar ...
GPCR–G fusion proteins
... Basic properties of GPCR–Ga fusion proteins Construction of fusion protein DNAs and structural properties of fusion proteins Fusion proteins are generated by linking the GPCR C-terminus, which is located intracellularly, to the Nterminus of Ga (Refs 1–6, 9–13, 18). This is achieved by fusing the ope ...
... Basic properties of GPCR–Ga fusion proteins Construction of fusion protein DNAs and structural properties of fusion proteins Fusion proteins are generated by linking the GPCR C-terminus, which is located intracellularly, to the Nterminus of Ga (Refs 1–6, 9–13, 18). This is achieved by fusing the ope ...
Protein phosphatases and the regulation of mitosis
... Structural studies have shown two different mechanisms for substrate recognition by PP1, through the binding of regulatory subunits. In the case of spinophilin, the binding site for PP1 is unstructured in solution, but on binding to PP1 it adopts an ordered conformation, which coordinates the cataly ...
... Structural studies have shown two different mechanisms for substrate recognition by PP1, through the binding of regulatory subunits. In the case of spinophilin, the binding site for PP1 is unstructured in solution, but on binding to PP1 it adopts an ordered conformation, which coordinates the cataly ...
Protein Folding at the Exit Tunnel
... Since Christian Anfinsen’s pioneering article on the relation between protein sequence and structure in 1954 (1) and his formulation of the thermodynamic hypothesis of protein folding in 1962, thousands of articles have been written on how proteins travel through energy landscapes and reach their na ...
... Since Christian Anfinsen’s pioneering article on the relation between protein sequence and structure in 1954 (1) and his formulation of the thermodynamic hypothesis of protein folding in 1962, thousands of articles have been written on how proteins travel through energy landscapes and reach their na ...
The Localization and Secretion of a Proteolytic
... rather lower yields on casein hydrolysate. Proteolytic enzymes were found in the culture filtrate when the organism was growing on casein but not when casein hydrolysate was present in the medium. The enzymes appeared first at the beginning of the exponential phase of growth and steadily increased u ...
... rather lower yields on casein hydrolysate. Proteolytic enzymes were found in the culture filtrate when the organism was growing on casein but not when casein hydrolysate was present in the medium. The enzymes appeared first at the beginning of the exponential phase of growth and steadily increased u ...
Assembly of AO and DHAS - Journal of Cell Science
... were quickly lysed and subjected to a single centrifugation to separate cytosol (and light membranes, the SUP fraction) from peroxisomes (and other organelles, the PEL fraction). Fig. 1B demonstrates that this step results in the release of 94% of a cytosolic marker, formaldehyde dehydrogenase, a me ...
... were quickly lysed and subjected to a single centrifugation to separate cytosol (and light membranes, the SUP fraction) from peroxisomes (and other organelles, the PEL fraction). Fig. 1B demonstrates that this step results in the release of 94% of a cytosolic marker, formaldehyde dehydrogenase, a me ...
BIOCHEMICAL ADAPTATION
... 2. Temperature alters the strength of the weak bonds that are critical to the conformation of macromolecules and to the conformational changes that occur during ligand binding and catalysis in enzymes. A decrease in temperature increases the stability of hydrogen bonds, van der Waals interactions, a ...
... 2. Temperature alters the strength of the weak bonds that are critical to the conformation of macromolecules and to the conformational changes that occur during ligand binding and catalysis in enzymes. A decrease in temperature increases the stability of hydrogen bonds, van der Waals interactions, a ...
Role of cryo-ET in membrane bioenergetics research
... distribution of protein complexes. Proteins on the unstacked membrane surfaces were larger and more dispersed than those on the stacked membranes. Through a combination of immunolabelling and biochemical methods, it was concluded that PSII was located primarily on the stacked membranes, whereas PSI ...
... distribution of protein complexes. Proteins on the unstacked membrane surfaces were larger and more dispersed than those on the stacked membranes. Through a combination of immunolabelling and biochemical methods, it was concluded that PSII was located primarily on the stacked membranes, whereas PSI ...
Delivering of Proteins to the Plant Vacuole—An Update
... PSV [4,17]. Contrarily to the initial observations, direct ER-to-Vacuole transport is not limited to storage proteins being already described for tonoplast aquaporins and processing enzymes [18,19]. Transport to the lytic vacuole occurs after recognition of the sorting signal by the receptor BP-80 ( ...
... PSV [4,17]. Contrarily to the initial observations, direct ER-to-Vacuole transport is not limited to storage proteins being already described for tonoplast aquaporins and processing enzymes [18,19]. Transport to the lytic vacuole occurs after recognition of the sorting signal by the receptor BP-80 ( ...
Choosing the Best Kinase Assay to Meet Your Research Needs
... Protein kinases are enzymes capable of transferring the γphosphate group from ATP to a serine, threonine or tyrosine residue in specific substrate proteins. These phosphorylation events modulate the activity of a vast number of proteins, including ion channels, transcription factors, phosphatases an ...
... Protein kinases are enzymes capable of transferring the γphosphate group from ATP to a serine, threonine or tyrosine residue in specific substrate proteins. These phosphorylation events modulate the activity of a vast number of proteins, including ion channels, transcription factors, phosphatases an ...
Journal of Medical Microbiology
... Sigma (from swine gut mucosal tissue) was coupled to horseradish peroxidase (POD-HS) as follows. POD was incubated with 0.1 M sodium periodate for 20 min at room temperature and dialysed overnight against 1 mM ...
... Sigma (from swine gut mucosal tissue) was coupled to horseradish peroxidase (POD-HS) as follows. POD was incubated with 0.1 M sodium periodate for 20 min at room temperature and dialysed overnight against 1 mM ...
Interaction between G proteins and accessory в subunits in
... regulate their biophysical properties and have also been suggested to antagonise the G protein inhibition of N-type (á1B), PÏQ-type (á1A) and á1E channels. Here we have examined the voltage-dependent involvement of the four neuronal isoforms (â1b, â2a, â3 and â4) in the process of G protein modulati ...
... regulate their biophysical properties and have also been suggested to antagonise the G protein inhibition of N-type (á1B), PÏQ-type (á1A) and á1E channels. Here we have examined the voltage-dependent involvement of the four neuronal isoforms (â1b, â2a, â3 and â4) in the process of G protein modulati ...
Package `PPInfer`
... Description Interactions between proteins occur in many, if not most, biological processes. Most proteins perform their functions in networks associated with other proteins and other biomolecules. This fact has motivated the development of a variety of experimental methods for the identification of ...
... Description Interactions between proteins occur in many, if not most, biological processes. Most proteins perform their functions in networks associated with other proteins and other biomolecules. This fact has motivated the development of a variety of experimental methods for the identification of ...
Dual Location of the Mitochondrial Preprotein
... also detected the upper band of Tim17:23 complex, but not complex I (Figure 2B). To confirm further the dual locations of both Tim23-2 and B14.7, in vitro protein uptake assays of radiolabeled proteins were performed and analyzed by BN-PAGE. The uptake of radiolabeled Tim23-2 into the Tim17:23 comple ...
... also detected the upper band of Tim17:23 complex, but not complex I (Figure 2B). To confirm further the dual locations of both Tim23-2 and B14.7, in vitro protein uptake assays of radiolabeled proteins were performed and analyzed by BN-PAGE. The uptake of radiolabeled Tim23-2 into the Tim17:23 comple ...
Protein S deficiency
... C4b-BP has a molecular weight of 570 kD and is composed of either six or seven identical α-chains (70 kDa) and either one (C4b-BPβ+) or no (C4b-BPα) β-chains (45 kDa), resulting in a spider-like structure held together by disulphide bonds. A binding site for C4b is located on each of the α-chains, w ...
... C4b-BP has a molecular weight of 570 kD and is composed of either six or seven identical α-chains (70 kDa) and either one (C4b-BPβ+) or no (C4b-BPα) β-chains (45 kDa), resulting in a spider-like structure held together by disulphide bonds. A binding site for C4b is located on each of the α-chains, w ...
Atomic contacts in protein structures. A detailed analysis of atomic
... Billions of years of evolution optimized proteins to fulfill their functions efficiently. Regardless whether the protein functions as enzyme, molecular motor, transport protein, or receptor, a prerequisite for optimal function is a fine-tuned structural and dynamical framework, either directly or in ...
... Billions of years of evolution optimized proteins to fulfill their functions efficiently. Regardless whether the protein functions as enzyme, molecular motor, transport protein, or receptor, a prerequisite for optimal function is a fine-tuned structural and dynamical framework, either directly or in ...
Vertebrate hairy and Enhancer of split related proteins
... hairy-like and E(spl)-like proteins have been named HES (hairy and enhancer of split) or her (hairy and enhancer of split related) in mammals and zebra®sh respectively, and numbered by the order of isolation. Hey proteins have also been named HRT or Hesr, or in the case of Hey2, Gridlock or CHF1, wh ...
... hairy-like and E(spl)-like proteins have been named HES (hairy and enhancer of split) or her (hairy and enhancer of split related) in mammals and zebra®sh respectively, and numbered by the order of isolation. Hey proteins have also been named HRT or Hesr, or in the case of Hey2, Gridlock or CHF1, wh ...
Human Dcp2: a catalytically active mRNA decapping enzyme
... and poorly conserved C-terminal extensions that make them much larger than their yeast counterpart (63±68 versus 26 kDa) (Figure 1A). While the similarity between hDcp1, hDcp1b and yeast Dcp1 is relatively low (20% identity shared between the yeast protein and either hDcp1 or hDcp1b in the conserved ...
... and poorly conserved C-terminal extensions that make them much larger than their yeast counterpart (63±68 versus 26 kDa) (Figure 1A). While the similarity between hDcp1, hDcp1b and yeast Dcp1 is relatively low (20% identity shared between the yeast protein and either hDcp1 or hDcp1b in the conserved ...
The TACC proteins: TACC-ling microtubule dynamics and
... the TACC domain. It is interesting to note that truncated proteins lacking the TACC domain do not localize to centrosomes or microtubules in Drosophila or Xenopus [12,42]. Interestingly, some data suggest that TACC proteins bind to the ends of microtubules. On the one hand, the high degree of accumu ...
... the TACC domain. It is interesting to note that truncated proteins lacking the TACC domain do not localize to centrosomes or microtubules in Drosophila or Xenopus [12,42]. Interestingly, some data suggest that TACC proteins bind to the ends of microtubules. On the one hand, the high degree of accumu ...
The Salmonella Effector Protein SopA Modulates Innate Immune
... The role of TRIM56 in the stimulation of innate immune responses is well documented [33, 34]. In contrast, TRIM65 has been reported to be a repressor of microRNA-guided mRNA silencing and its potential involvement in innate immune responses is uncertain [35, 36]. To better understand the potential s ...
... The role of TRIM56 in the stimulation of innate immune responses is well documented [33, 34]. In contrast, TRIM65 has been reported to be a repressor of microRNA-guided mRNA silencing and its potential involvement in innate immune responses is uncertain [35, 36]. To better understand the potential s ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.