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Goal 3.05 Examine the Theory of Evolution by Natural
Goal 3.05 Examine the Theory of Evolution by Natural

... HEARTIER /LARGER-STRONGER. 5. This figure shows a RESTRICTION ENZYME cutting a DNA strand, which is one of the first steps in producing RECOMBINANT DNA. ...
Final
Final

... Which of the following is characteristic of a plasmid? Circle all that apply a. b. c. d. ...
code sequence practice
code sequence practice

... Old DNA strand: A G C T A T G A C New DNA strand: Transcription – making mRNA from DNA 2. If this is your original DNA strand, what is the mRNA sequence that is synthesized? DNA Strand: C A G T G C A T T mRNA strand: 3. Now go backwards, if you are given the following mRNA strand, write the DNA stra ...
Biology 4.15 PCR
Biology 4.15 PCR

... Using the technique called polymerase chain reaction (PCR), researchers are able to create vast quantities of DNA identical to trace samples. This process is also known as DNA amplification. ...
This examination paper consists of 4 pages
This examination paper consists of 4 pages

... make up more than 40% of the human genome can be as short as 100 bp occur in prokaryotes and in eukaryotes transpose conservatively code for a transposase enzyme ...
DNA and Protein Synthesis Concept Questions
DNA and Protein Synthesis Concept Questions

... 1. What are nucleotides? Describe their structure. 2. Describe how the work of Hershey and Chase, Chargaff, and Wilkins and Franklin contributed to the discovery by Watson and Crick of the double helix. 3. While you’re trying to enjoy your lunch one day, your friend says she is working on an art pro ...
Introduction to the biology and technology of DNA microarrays
Introduction to the biology and technology of DNA microarrays

... • Nucleus: membrane enclosed structure which contains chromosomes, i.e., DNA molecules carrying genes essential to cellular function. • Cytoplasm: the material between the nuclear and cell membranes; includes fluid (cytosol), organelles, and various membranes. • Ribosome: small particles composed of ...
Section 2
Section 2

... The bases in the DNA molecule can be thought of in much the same way as the LETTERS in the alphabet. From the letters in the alphabet it is possible to make hundreds of thousands of different combinations that are called _”WORDS”_. The four nitrogen bases (A, T, C, G) do the same thing; they can com ...
DNA, genes and chromosomes
DNA, genes and chromosomes

... causative agent of cholera, has two circular chromosomes.) The chromosome - together with ribosomes and proteins associated with gene expression - is located in a region of the cell cytoplasm known as the nucleoid. The genomes of prokaryotes are compact compared with those of eukaryotes, as they lac ...
Unit 10: Cell Biology, Molecular Biology, DNA NGSS Priority
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... 1. How can the structure and function of plasmids be described? 2. How are restriction enzymes used to create designer plasmids? 3. How can a plasmid map be created and analyzed? 4. What are current uses of transgenic organisms? 5. What steps are required to transform E.coli using the pGLO plasmid? ...
Biology 340 Molecular Biology
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... Another technical concern in construction of knockouts: Growth of ES cells and insertion of genes: 1. ES cells are derived from mouse embryos; they are not from established cell lines, thus are technically difficult to grow. 2. ES cells are subcultured on a layer of feeder cells, cells that assist t ...
DNA fingerprinting
DNA fingerprinting

... • Among these hypervariable regions are variable number tandem repeat sequences (VNTR) • The number of the VNTRs can vary significantly from individual to individual • In humans such sequences are often bordered by restriction endonuclease sites. • The fragment sizes resulting from digestion depend ...
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You Light Up My Life

... • What is the transforming material? • Cell extracts treated with proteindigesting enzymes could still transform ...
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... around I5 times lower than those in crosses homozygous for the recessive eallele. The recombination-3 gene doer not control recombination a+ the hirtidine-1 locus which is linked to aminotion on chromosome V or at the hirtidine-3 locus on chromorome I (Jho 1967 Genetics 57:365), indicating that its ...
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... forms  words  and  sentences  that  help  you  understand  the  author’s  meaning.   The  alphabet  of  your  DNA  only  has  4  letters:  A,C,G,  and  T!  These  letters  aren’t   the  same  as  the  letters  of  our  alphabet;  th ...
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... DNA of another organism.  Recombinant DNA technology is also known as gene splicing. ...
6.2 Recombinant DNA Technology
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... Steps (2)  Fragments of human DNA and plasmid mixed together and join  Plasmids enter the bacterial cells, copy themselves, carry recombinant DNA into bacteria  Bacteria express gene, synthesize the human protein, can be used for treatments, vaccines, or other purposes ...
Biotechnology
Biotechnology

... DNA Cloning • Cloning employs plasmids, small circles of DNA found in prokaryotic cells that are supplemental to the bacterial cells main DNA • Plasmids are removed from host cells and cut with restriction enzymes. The gene to be copied is mixed with the cut plasmids and complimentary ends align. D ...
PRE-AP Stage 3 – Learning Plan
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... SCAFFOLD: Students will identify the components of DNA and describe how genetic information is carried in DNA. After identifying the components of the structure of DNA, students will explain how DNA is transcribed and translated into amino acids to make proteins. ACCELERATE: PREAP – purines, pyrimid ...
Topic 4: Genetics - wfs
Topic 4: Genetics - wfs

... 30000 genes. Not only did the project strive to find the total genes but it attempted to find each gene’s location and each gene’s base sequence. 6. Benefits of the Human Genome Project include the ability to study how genes influence human development, the easier identification of genetic diseases, ...
Endocrinology 3
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... Protooncogenes = Normal gene precursors of oncogenes I V Mutational Agents I V Oncogenes = Gene associated with abnormal cell growth ...
Unit 5 Review
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... _______ Daughter strands are formed using complementary base pairing. _______ DNA unwinds. _______ The DNA of the daughter strands winds with together with its parent strand. 14. Show the complimentary base pairing that would occur in the replication of this short DNA molecule. Use a colored pencil ...
9.1 Manipulating DNA
9.1 Manipulating DNA

... Biotechnology relies on cutting DNA at specific places. ...
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9.1 Manipulating DNA KEY CONCEPT Biotechnology relies on cutting DNA at specific places.

... Biotechnology relies on cutting DNA at specific places. ...
DNA_Project - Berkeley Cosmology Group
DNA_Project - Berkeley Cosmology Group

... from phosphate, a sugar, and one of four nitrogenous bases. The four nitrogenous bases are adenine, thymine, cytosine, and guanine. Based on this cytosine bonds with guanine, and thymine binds with guanine to form bonds between the nucleotides thus creating a strand of DNA. DNA is used in a cell to ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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