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WLHS / AP Bio / Monson
WLHS / AP Bio / Monson

... RECOMMENDED: Try (or at least look over) the “Self-Quiz” multiple choice questions for each chapter! ALSO, review all study questions from CH 16-18. REVIEW QUESTIONS: (some may done on a separate sheet of paper and attached) 1) Explain (or use a sketch/diagram) how Hershey & Chase used radioactively ...
Biotechnology and Gel Electrophoresis
Biotechnology and Gel Electrophoresis

... In DNA Fingerprinting, the DNA of an organism is cut up into fragments using restriction enzymes producing a large number of fragments of DNA Because no two individuals have identical DNA, no two individuals will have the same length fragments This technique allows us to identify families because th ...
• Double helix -- twisted ladder shape of DNA, like spiral staircase
• Double helix -- twisted ladder shape of DNA, like spiral staircase

... Which letters bind with which? A - T, G - C ...
Interaction of β-Cyclodextrin with DNA-Bases
Interaction of β-Cyclodextrin with DNA-Bases

... DNA strand so they can traverse the pore one at a time. The different nucleotides then bind to cyclodextrin attached to the inside of the pore [1]. The different bases are discriminated by the change they induce in the amplitude of the current carried by aqueous ions passing through the pore. Severa ...
Evolution process by which species change over time
Evolution process by which species change over time

... DNA Evidence of Evolution • DNA is an organisms genetic material that is responsible for its characteristics and traits • Scientists have found common DNA sequencing or DNA strands in many species indicating they came from a common ancestor • Humans and Chimps have 99% similar DNA, alike in genetic ...
Target-triggered DNA three-way junction superstructure and
Target-triggered DNA three-way junction superstructure and

... in urgent need [1]. We herein report a sensor design that integrates DNA three-way junction and catalytic activities of deoxyribozyme (DNAzyme) for sensitive and specific identification of dual target sequences [genes encoding hemagglutinin (HA) and neuraminidase (NA)] simultaneously. By implementat ...
consumer perceptions of food biotechnology
consumer perceptions of food biotechnology

... Molecular biology Study of genes and gene replication, mutation and expression Genome is the collection of all base pairs within the cell Human Genome project started in 1980s ...
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Genetics EQ

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DNA Barcoding
DNA Barcoding

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Recombinant DNA.
Recombinant DNA.

... 3. Hydrogen bonding between bases ...
I.
I.

... I. Questions (50%) 1. What would happen if the different tRNAs in cells could bind to just any amino acid? How does the specificity of tRNA for particular amino acids maintain the integrity of the genetic information? (10%) ...
Biotechnology
Biotechnology

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Leq: what is cloning and how is it done?
Leq: what is cloning and how is it done?

... improve tools for data analysis, transfer related technologies to the private sector, and address the ethical, legal, and social issues (ELSI) that may arise from the project. Though the HGP is finished, analyses of the data will continue for many years http://www.ornl.gov/sci/techresources/Human_Ge ...
7 October 2015 The Royal Swedish Academy of Sciences has
7 October 2015 The Royal Swedish Academy of Sciences has

... information. Their work has provided fundamental knowledge of how a living cell functions and is, for instance, used for the development of new cancer treatments. Each day our DNA is damaged by UV radiation, free radicals and other carcinogenic substances, but even without such external attacks, a D ...
Tibor Readings - Molecular Neuroscience Part I.
Tibor Readings - Molecular Neuroscience Part I.

Pretest and Post Test Questions
Pretest and Post Test Questions

... C) 44 D) 56 E) 72 Answer: A 8) Where does DNA replication occur in eukaryotes? A) cytoplasm B) ribosome C) nucleus D) vacuole E) rough endoplasmic reticulum Answer: C 9) Where does DNA replication occur in prokaryotes? A) cytoplasm B) ribosome C) nucleus D) vacuole E) rough endoplasmic reticulum Ans ...
Chapter 30 DNA replication, repair and recombination
Chapter 30 DNA replication, repair and recombination

Document
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... A certain Drosophila protein-coding gene has one intron. If a large sample of null alleles of the gene is examined, will any of the mutant sites be EXPECTED: First, what is a null mutation?! A null mutation is one that results in complete absence of function of the geneResults in no protein functio ...
What are multiple alleles
What are multiple alleles

... from around the developing fetus is extracted using a syringe. Fetal cells from the fluid are analyzed to see if there are any chromosomal abnormalities. ...
starter - Dunlap CUSD #323
starter - Dunlap CUSD #323

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Ch. 16 - Harford Community College
Ch. 16 - Harford Community College

... • In conjugation, an F factor-containing "male" cell transfers DNA to an F- cell. • F+ cells transfer only the F plasmid. • The F factor of an Hfr cell, which is integrated into the bacterial chromosome, brings some chromosomal DNA along with it when it transfers to an F- cell. • R plasmids confer ...
Previously in Bio308
Previously in Bio308

... How does RNA polymerase work and what does it make? How does it know where to start and stop? How does a ribosome work and what does it make? How does it know where to start and stop? If the DNA in every cell in your body is the same why don't your adipose (fat) cells secrete epinephrine? If the DNA ...
The DNA Song
The DNA Song

... made of a sugar (deoxyribose), a phosphate group, and one of 4 bases: cytosine, guanine, thymine, and adenine. These nucleotides link together by covalent (strong) bonds between the sugars and phosphate groups to form strands. Two of the strands link together at the bases with hydrogen (weak) bonds. ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... -----------------------------------------------------------------------------------------------------------------This exam consists of 40 multiple choice questions worth 2.5 points each. On the separate testing form, please fill-in the single best choice for each question. Be sure to fill-out your s ...
Final
Final

... Which of the following is characteristic of a plasmid? Circle all that apply a. b. c. d. ...
< 1 ... 690 691 692 693 694 695 696 697 698 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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