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Genetic Engineering
Genetic Engineering

... • Breed only those plants or animals with desirable traits ...
Exploratorium Presentation
Exploratorium Presentation

... What is bacterial transformation? Transformation is the alteration of cells by the incorporation of foreign DNA into the cell ...
Name: Date: Per:______ DNA Guided Reading There are two types
Name: Date: Per:______ DNA Guided Reading There are two types

... Many genetic disorders are caused by recessive genes. It is a disease or condition that results from mutations. If an offspring receives two recessive alleles from parents, the child inherits the disease. If a person is heterozygous, he/she does not show the symptoms. Sickle cell anemia is one such ...
Name
Name

... The biochemical pathway for the compounds IGP, AA, IN, and TRP based on these data is a.  IGP  AA  IN  TRP b.  AA  IGP  IN  TRP c.  TRP  IN  IGP  AA d.  AA  IN  IGP  TRP 14. E. coli DNA polymerase I has an essential function in DNA replication. Which of the following is that function ...
Press Release - Max Delbrück Center for Molecular Medicine
Press Release - Max Delbrück Center for Molecular Medicine

... The Berlin Institute for Medical Systems Biology (BIMSB) of the Max Delbrück Center for Molecular Medicine (MDC) in Berlin, Germany, will be the first academic research institution in Continental Europe to acquire a novel DNA sequencer enabling the sequencing of single DNA molecules in real time. Th ...
16.2 Biotechnology Products
16.2 Biotechnology Products

of human DNA responsible for metastasis in breast cancer
of human DNA responsible for metastasis in breast cancer

... Metastasis is the process whereby cancer cells spread from their site of origin to distant sites of the body. Most primary tumours, including those of the brcast are thought to arise in a benign form and, at a late stage in development, to acquire multiple genetic altcrdtions that pmmotc disseminati ...
Lecture 14 – 10/5 – Dr. Wormington
Lecture 14 – 10/5 – Dr. Wormington

... already has 7x106 oocytes arrested in Meiotic Prophase I •At birth, this has already declined to ~106 oocytes •Most oocytes undergo apoptosis •Only 1-2 oocytes typically resume meiosis and proceed to Metaphase II during each monthly ovulation between the ages of 12–50 •12–50 years may elapse between ...
Biotechnology and Mutation Quiz key
Biotechnology and Mutation Quiz key

... certain types of diseases and pests. How might these genetically-engineered crops impact the environment? A. They will help the environment by causing pest species to become extinct. B. They will harm the environment by introducing synthetic genes into other plants. C. They will help the environment ...
Laboratory #1 Lecture Guide: Forensic DNA Fingerprinting
Laboratory #1 Lecture Guide: Forensic DNA Fingerprinting

... 2. Why must we always load the DNA on the negative end of the chamber? 3. What is the relationship between the gel’s density and the movement of the DNA ...
The Avery and Hershey-Chase Experiments
The Avery and Hershey-Chase Experiments

... Avery Experiments (cont.) – The properties of the transforming principle resembled those of DNA in several ways: • When the purified principle was analyzed chemically, the array of elements agreed closely with DNA • When spun at high speeds, the transforming principle migrated at the same level (de ...
File
File

... c. Cells provide structure for a body, take in nutrients from food, convert nutrients into energy, and carry out specialised functions. ...
DNA REVIEW SHEET
DNA REVIEW SHEET

... 15. Where is an anticodon located? 16. A codon that has no anticodon match would be called a ___________________. 17. What does DNA polymerase do? 18. Anything ending in –ase would be classified as an ____________________> 19. What 3 things make up DNA? 20. DNA is compared in structure to what? 21. ...
DNA - The Double Helix
DNA - The Double Helix

... sugar is deoxyribose. Color all the phosphates pink (one is labeled with a "p"). Color all the deoxyriboses blue (one is labeled with a "D") . The rungs of the ladder are pairs of 4 types of nitrogen bases. The bases are known by their coded letters A, G, T, C. These bases always bond in a certain w ...
File
File

... 3 types of mutations: • 2. Frameshift mutations: a single base is added or deleted from DNA – This would cause every codon to be wrong from that point on in protein coding – Example: • THE CAT ATE THE FAT RAT • THE ATA TET HEF ATR ATT ...
sdfgdfgdsfgdfgdfg
sdfgdfgdsfgdfgdfg

... The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isolated nuclei. The PI intercalates into the major groove of double-stranded DNA and produces a highly fluorescent adduct that can be excited at 488 nm with a broad emissi ...
II. Transposable Elements in Bacteria Transposable Elements are
II. Transposable Elements in Bacteria Transposable Elements are

... promotion and regulation of their transposition, typically the gene for the so-called transposase enzyme. IS elements are between 700 - 2,000 bp in length and are characterized by short, terminal, inverted repeat sequences with the ORF or ORF's in between. They are normal constituents of many bacter ...
Genetic Engineering
Genetic Engineering

... desirable traits ...
Product Information Sheet - Sigma
Product Information Sheet - Sigma

... produce fragments in a size range of 587 to 831 base pairs. This range has been shown to be the most effective for hybridizations. The material is monitored during sonication by electrophoresis in order to determine the size range. Once sonication is complete, the material is denatured by boiling. T ...
No Slide Title
No Slide Title

... – 2. construct physical maps of all 24 chromosomes (22 autosomes, X and Y) – 3. sequence entire genome by 2005 All goals ahead of schedule – first draft map published in 2001 ...
PowerPoint
PowerPoint

... DNA of another organism.  Recombinant DNA technology is also known as gene splicing. ...
How many fragments of DNA can be assembled in one reaction
How many fragments of DNA can be assembled in one reaction

... NEBuilder® HiFi DNA Assembly: Bridging dsDNA with a ssDNA Oligo Learn how NEBuilder® HiFi DNA Assembly bridges dsDNA with a ssDNA oligo. ...
gene
gene

... What features do Peter and Chris share? How are they different? ...
DNA sequencing File
DNA sequencing File

JF lect 5 12
JF lect 5 12

... (1) Evidence that Genes are located on Chromosomes 1902 – McClung – a particular chromosome (X) determines sex in insects (XO = male; XX = female) 1903 – Sutton and Boveri – chromosomes behave just like the ‘unit factors’ described by Mendel 1910 – Morgan – the “white” eye color gene of Drosophila i ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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