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1.3. Identity: Molecules and Cells Study Guide
1.3. Identity: Molecules and Cells Study Guide

... DNA stands for deoxyribonucleic acid. Its name comes from the fact that the sugar in it is deoxyribose and it is made up of building blocks of nucleic acids (just like RNA). It is a double-stranded helical molecule that the chromosomes in the nucleus of our cells are made of. DNA makes up genes, whi ...
DNA and RNA
DNA and RNA

... with Thymine  A-T or T-A (2 H bonds)  Cytosine can bond only with Guanine  C-G or G-C (3 H bonds)  This is called the BASE PAIR RULE ...
Microbiology Babylon university 2nd stage pharmacy collage
Microbiology Babylon university 2nd stage pharmacy collage

... Restriction & Other Constraints on Gene Transfer Restriction enzymes (restriction endonucleases) provide bacteria with a mechanism to distinguish between their own DNA and DNA from other biologic sources. These enzymes hydrolyze DNA at restriction sites determined by specific DNA sequences ranging f ...
DNA and RNA ppt
DNA and RNA ppt

... with Thymine  A-T or T-A (2 H bonds)  Cytosine can bond only with Guanine  C-G or G-C (3 H bonds)  This is called the BASE PAIR RULE ...
Unit A Topic 3
Unit A Topic 3

... 7. The arrangement of the four chemicals (G) ________________ , (C) _______________ , (A) _______________________ and (T) _________________________ form a code that cells can read. 8. The __________________________ is based on arranging the four chemical ‘letters’ into instructions that describe how ...
Genomics * Reading What we Can*t See
Genomics * Reading What we Can*t See

Teacher Resource 8: Genetic engineering
Teacher Resource 8: Genetic engineering

... and digital cameras will need to be booked prior to the lesson. A range of art/craft resources to model each stage of genetic engineering will need to be collected and made available for learners. ...
Protein Synthesis - Simon Technology
Protein Synthesis - Simon Technology

... predict the physical characteristics of an organism based on its genetic make up. understand the general pathway by which ribosomes make proteins. explain the causes of gene and chromosomal mutations in multicellular organisms. understand how changes in DNA sequences can cause changes in the protein ...
Protein Synthesis
Protein Synthesis

... predict the physical characteristics of an organism based on its genetic make up. understand the general pathway by which ribosomes make proteins. explain the causes of gene and chromosomal mutations in multicellular organisms. understand how changes in DNA sequences can cause changes in the protein ...
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Jan. 28 Bio II Answer to warm up Protein Synthesis

... Grade is based on accuracy and aesthetic appeal NO LATE PROJECTS ACCEPTED! ...
genetic engineering
genetic engineering

...  vector is a DNA segment that provides input corresponding gene into the cell and its expression  the most suitable vector is just phage DNA  gene can be artificially incorporated by specific enzymes into phage DNA ...
Transcription is the process by which RNA polymerase copies a
Transcription is the process by which RNA polymerase copies a

... summarized on the vocabulary page. In addition to figuring out the structure of DNA these two scientists also noticed that DNA is like a zipper – in it can be split into a two single strands by separating the hydrogen bonds between the base pairs. Check out this video http://www.youtube.com/watch?v= ...
DNA Authorization - Donahue Funeral Home
DNA Authorization - Donahue Funeral Home

... 2. Please visit the DNA Memorial website. 3. Notify us if you wish to consider DNA banking.  Would you like an email reminder? ...
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2nd problem set

... 1. Imagine you are sequencing the DNA molecule shown above. Assume the primer 5’ GATGCCT 3’ is used to initiate DNA synthesis. You have a tube containing template, primer, millions of ACGT nucleotides and millions of dideoxyC nucleotides. (p. 387-393 of your textbook has a good review if you are hav ...
Genetics 3500 winter Test ii_ansers
Genetics 3500 winter Test ii_ansers

Hypercholesterolemia Questions KEY
Hypercholesterolemia Questions KEY

... It is found on chromosome 19 and only one copy must be present for a person to have the disease. Both homozygous dominant as well as heterozygous individuals will have the disease. However, a person that is homozygous dominant will have a worse case of the disease. ...
One copy from each parent Each parent passes on a “mixed copy”
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Prepractical demo_SF_Class_2009

... Can you see anything unusual about these sequences? Why don’t they cleave the bacteria’s DNA?   We want an enzyme that will cleave one allele of the TAS2R38 sequence but not the other - need polymorphism itself to be part of the cleavage site ...
Biotechnology
Biotechnology

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Cow DNA: How DNA Controls the Workings of the Cell
Cow DNA: How DNA Controls the Workings of the Cell

... 5. Diabetes is a disease characterized by the inability to break down sugars. Often a person with diabetes has a defective DNA sequence that codes for the making of the insulin protein. Suppose a person has a mutation in their DNA and the first triplet for the insulin gene reads T A T. The normal ge ...
Aim 24: How does DNA code for the production of proteins through
Aim 24: How does DNA code for the production of proteins through

... Aim 24: How does DNA code for the production of proteins through the process of translation? Recall: Protein synthesis begins with the transcription of DNA to mRNA (The RNA that is synthesized from a DNA molecule). This process occurs in the nucleus, allowing the mRNA strand to leave the nucleus wit ...
Study Guide- 3.3-3.4-3.5-7.1-7.2-7.3-7.4
Study Guide- 3.3-3.4-3.5-7.1-7.2-7.3-7.4

... 3) Draw a nucleotide and label the following parts: base, deoxyribose, phosphate, 5’ end, 3’ end, Hydrogen bond, covalent bond. ...
Document
Document

... Example: When you write a letter, you put together words using different letters of the alphabet. With twenty-six letters you can say anything you want. It is important that the letters go in the right order. This sentence stops making sense whenthaliekrnviserhflker are in the wrong order. When you ...
Biotechnology
Biotechnology

... gene is cloned (more copies are made). ...
Vector Construction II - Department of Plant Sciences
Vector Construction II - Department of Plant Sciences

... Multisite Gateway allows several DNA fragments to be cloned into a single construct ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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