Genetic Engineering
... from a female of the same species (known as the "egg donor"). In the lab, a scientist extracts and discards the nucleus of the egg cell, which is the part of the cell that contains the egg donor's genes. The scientist then inserts the somatic cell from the genetic donor into the egg and "fuses" the ...
... from a female of the same species (known as the "egg donor"). In the lab, a scientist extracts and discards the nucleus of the egg cell, which is the part of the cell that contains the egg donor's genes. The scientist then inserts the somatic cell from the genetic donor into the egg and "fuses" the ...
Biotechnological Methods and Products
... •May not be able to modify or export complex mammalian proteins ...
... •May not be able to modify or export complex mammalian proteins ...
GENE MUTATION = POINT MUTATION at the DNA level: at the level
... Spontaneous mutations: a mutation that occurs in the absence of known mutagens • uncorrected errors* that occur during DNA replication, repair or recombination • spontaneous lesions that occur to the DNA molecule under normal physiological conditions and that are not repaired by the cell’s DNA exci ...
... Spontaneous mutations: a mutation that occurs in the absence of known mutagens • uncorrected errors* that occur during DNA replication, repair or recombination • spontaneous lesions that occur to the DNA molecule under normal physiological conditions and that are not repaired by the cell’s DNA exci ...
“Ins and Outs” of Restrictions Enzymes
... – First letter from the genus – Second two letters from the species – Numbers indicate the order from which they were isolated from single strains ...
... – First letter from the genus – Second two letters from the species – Numbers indicate the order from which they were isolated from single strains ...
Hershey-Chase Experiment
... Early genetics had several basic problems to solve, and chief among them was to determine what exactly was the genetic material inside cells. This was solved by two scientists, Alfred Hershey and Martha Chase. This discovery pointed the way for several subsequent studies that opened a new generation ...
... Early genetics had several basic problems to solve, and chief among them was to determine what exactly was the genetic material inside cells. This was solved by two scientists, Alfred Hershey and Martha Chase. This discovery pointed the way for several subsequent studies that opened a new generation ...
Principles of genetic engineering
... What is genetic engineering • Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype. • Various kinds of genetic modification are possible: inserting a foreign gene from one sp ...
... What is genetic engineering • Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype. • Various kinds of genetic modification are possible: inserting a foreign gene from one sp ...
Genetic engineering – stepping stones
... Draw a path across the stepping stones in the correct order for each process. 1) Dolly the sheep – cloning technique ...
... Draw a path across the stepping stones in the correct order for each process. 1) Dolly the sheep – cloning technique ...
24 Applied genetics
... (a) Show how a plant breeder would cross these varieties to produce a high yielding, short stemmed variety. (b) Explain why this variety would not breed true. 2 Choose from the list of words below, to complete the following sentence. In genetic engineering, a …..A …..from one organism is introduced ...
... (a) Show how a plant breeder would cross these varieties to produce a high yielding, short stemmed variety. (b) Explain why this variety would not breed true. 2 Choose from the list of words below, to complete the following sentence. In genetic engineering, a …..A …..from one organism is introduced ...
Objective - Central Magnet School
... • Why is Taq polymerase used in PCR instead of human polymerase? ...
... • Why is Taq polymerase used in PCR instead of human polymerase? ...
key
... 1. A critical feature of cloning plasmids is the presence of a selectable marker such as antibiotic (or ampicillin or …) resistance. 2. Northern blotting is a technique in which RNA is fractionated on a gel, and transferred to a membrane. The RNA attached to the membrane is incubated with a labeled ...
... 1. A critical feature of cloning plasmids is the presence of a selectable marker such as antibiotic (or ampicillin or …) resistance. 2. Northern blotting is a technique in which RNA is fractionated on a gel, and transferred to a membrane. The RNA attached to the membrane is incubated with a labeled ...
File - Intermediate School Biology
... 3. Diagnostic test for changed genes 4. (a) Shields the –ve DNA from the +ve proteins causing the DNA to clump. (b) Inactivates any enzymes not denatured.(c) removes cellular debris ( cell walls and membranes) (d) removes the protein associated with DNA. (e) DNA is insoluble in ice cold ethanol and ...
... 3. Diagnostic test for changed genes 4. (a) Shields the –ve DNA from the +ve proteins causing the DNA to clump. (b) Inactivates any enzymes not denatured.(c) removes cellular debris ( cell walls and membranes) (d) removes the protein associated with DNA. (e) DNA is insoluble in ice cold ethanol and ...
organization of chromatin and the fate of a cell
... not well understood. We now know that the fate of a cell is not just decided by the sequence of the DNA but also by the “state” of its chromatin. Chromatin is a 3-dimensional active assembly of DNA bound by many proteins (a set of biopolymer molecules). Chromatin can be assembled in multiple ways. A ...
... not well understood. We now know that the fate of a cell is not just decided by the sequence of the DNA but also by the “state” of its chromatin. Chromatin is a 3-dimensional active assembly of DNA bound by many proteins (a set of biopolymer molecules). Chromatin can be assembled in multiple ways. A ...
Translation RNA Single stranded Does not contain thymine but has
... that results from uncontrolled, abnormal cell division Benign – a tumor that remains within a mass Malignant tumor- uncontrolled dividing cells that invade and destroy healthy tissue elsewhere in the body Metastasis – spread of cancer cells beyond ...
... that results from uncontrolled, abnormal cell division Benign – a tumor that remains within a mass Malignant tumor- uncontrolled dividing cells that invade and destroy healthy tissue elsewhere in the body Metastasis – spread of cancer cells beyond ...
Biotechnology - BeautyinScience.com
... Selective breeding has been used by humans for thousands of years to increase the incidence of desirable traits from a variable population and produce domestic animals and crop plants. Dog breed characteristics are maintained by inbreeding between dogs of the same characters. Excessive inbreeding al ...
... Selective breeding has been used by humans for thousands of years to increase the incidence of desirable traits from a variable population and produce domestic animals and crop plants. Dog breed characteristics are maintained by inbreeding between dogs of the same characters. Excessive inbreeding al ...
Notes: Meiosis
... Why is variation necessary? 1. To increase the chance of an individual’s survival and, therefore, whole populations of that species. 2. Create challenges for natural predators of that species. Sources of Variation: 1. Recombinant DNA = DNA in sperm + DNA in egg = new combination of DNA in zygot ...
... Why is variation necessary? 1. To increase the chance of an individual’s survival and, therefore, whole populations of that species. 2. Create challenges for natural predators of that species. Sources of Variation: 1. Recombinant DNA = DNA in sperm + DNA in egg = new combination of DNA in zygot ...
DNA
... • Similar to DNA, but lets list the differences: • RNA is single stranded and is made up bases marked with letters- C-A-U-G • The T in DNA is substituted for a U in RNA. • This is because Thymine is only found in DNA, while Uracil is only found in RNA. ...
... • Similar to DNA, but lets list the differences: • RNA is single stranded and is made up bases marked with letters- C-A-U-G • The T in DNA is substituted for a U in RNA. • This is because Thymine is only found in DNA, while Uracil is only found in RNA. ...
What is the most likely path of inheritance?
... the possible blood phenotypes for Bernie? Genotypes for all? ...
... the possible blood phenotypes for Bernie? Genotypes for all? ...
Biotechnology webquest
... 7. Make 3 sketches; a) Before DNA is cut b) After it is cut, and c) after it is pasted together. (Include nitrogen bases and which type of enzyme is used at each stage.) a) b) c) ...
... 7. Make 3 sketches; a) Before DNA is cut b) After it is cut, and c) after it is pasted together. (Include nitrogen bases and which type of enzyme is used at each stage.) a) b) c) ...
Chapter 13
... Many copies of a desired gene can be cloned and its product harvested This is accomplished by using a cloning vector – an organism that contains the desired gene and can multiply rapidly – this organism is usually a bacterium Vector = carrier http://www.bioteach.ubc.ca/TeachingResources/Appl ication ...
... Many copies of a desired gene can be cloned and its product harvested This is accomplished by using a cloning vector – an organism that contains the desired gene and can multiply rapidly – this organism is usually a bacterium Vector = carrier http://www.bioteach.ubc.ca/TeachingResources/Appl ication ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.