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... genetic crosses. b. determine the actual outcomes of genetic crosses. c. determine which species should be used in genetic crosses. d. decide which organisms are best to use in genetic crosses. ...
EOC Vocab Review Terms
EOC Vocab Review Terms

... 1. ___Part of the experiment that does not contain the variable 2. ___Testable explanation for a problem 3. ___The factor in the experiment to be tested ...
forensic_biology
forensic_biology

... nucleotide "letters" A (adenine), C (cytosine), T (thymine), and G (guanine). SNP variation occurs when a single nucleotide, such as an A, replaces one of the other three nucleotide letters—C, G, or T. Each person's genetic material contains a unique SNP pattern that is made up of many different gen ...
CSC 121 Computers and Scientific Thinking David
CSC 121 Computers and Scientific Thinking David

... e.g., predict the toxicity of a new drug based on a chemical/biological model as opposed to animal testing e.g., study brain trauma using a neural network model ...
Arrays
Arrays

... to detect a unique, complementary DNA or RNA molecule. ...
EDVOTEK 225 DNA Fingerprinting
EDVOTEK 225 DNA Fingerprinting

... recognition sites. – Alleles result in alternative expressions of genetic traits, dominant or recessive – Two copies of gene at given locus, mom & dad – Alleles have differences in base sequences – Mutations and deletions occur which eliminate palindromic site (figure 2) ...
Lecture Slides - Computer Science
Lecture Slides - Computer Science

... Termination DNA ...
Producing the Bovine Growth Hormone
Producing the Bovine Growth Hormone

... including bovine growth hormone, or BGH. This DNA is found in the chromosomes in each cell's nucleus. To clone the BGH gene, DNA is taken from the cow cell's nucleus and cut with a restriction enzyme that leaves “sticky ends” on either side of the BGH gene. “Sticky ends” are so-named because they ar ...
PCR - Polymerase Chain Reaction
PCR - Polymerase Chain Reaction

... more specific, higher temps result in less mismatch – more specific replication ...
Topics covered on this exam include: cellular respiration
Topics covered on this exam include: cellular respiration

... 1. Compare and contrast DNA with RNA. How do they differ structurally? How are their functions different? 2. What are the components of a single nucleotide? Dow we find nucleotides in both RNA and DNA? 3. Be able to go between DNA  DNA, DNA  RNA and RNA  RNA. 4. What are the three types of RNA? W ...
DNA Extraction
DNA Extraction

... resulting DNA can be spooled (wound) on a stirring rod and pulled from the solution at this point. The extraction and purification of DNA are of primary importance to the field of biotechnology and forensics. DNA extraction allows for analysis including the detection of genetic disorders, identifica ...
medical genetics what is medical genetics?
medical genetics what is medical genetics?

... DNA polymerase is one of the key replication enzymes. It travels along the single DNA strand, adding free nucleotides to the 3' end of the new strand.( 3' and 5' referred to no. of the carbon atom in the pentose sugar to which the base is attached). Nucleotides can be added only to this end of the s ...
Chapter 6
Chapter 6

... four nitrogenous bases. DNA is double stranded, with both strands oriented “anti-parallel” to each other (Figure 6.1c). The two DNA strands are held together by hydrogen bonding between complementary bases (called base-pairing). Because A must pair with T, and C must pair with G, the bases on one st ...
Protein Synthesis Reading
Protein Synthesis Reading

... and parts of individual cells. The proteins that are made largely determine how you look. The proteins that will be made for your body are determined by the sequence of DNA in the nucleus. Chromosomes are composed of genes, which is a segment of DNA that codes for a particular protein, which in turn ...
2.5 Genetics - Rocoscience
2.5 Genetics - Rocoscience

... The process of making a protein using the mRNA code a template A haploid sex cell which is capable of fusion The fusion of 2[haploid] gametes to form a [diploid] zygote An alternative form of a gene Has identical alleles [for a trait] Has different alleles [for a trait] The genetic make-up of an ind ...
DNA to RNA
DNA to RNA

... information you need—shorter/simpler Think of it like this: DNA = master copy RNA = blueprints…you don’t need the blueprints for the whole house to build the foundation ...
Applied genetics - questions
Applied genetics - questions

... (a) Show how a plant breeder would cross these varieties to produce a high yielding, short stemmed variety. (b) Explain why this variety would not breed true. 2 Choose from the list of words below, to complete the following sentence. In genetic engineering, a …..A …..from one organism is introduced ...
DNA Discovery
DNA Discovery

... the appropriate machinery (enzymes and ribosomes) for us to produce proteins from a specific gene  insulin •Bacteria have small circular pieces of DNA called plasmids within their cytoplasm ...
PPT# 4 Notes: Mutations and Regulation     ...  Date______________Per._______
PPT# 4 Notes: Mutations and Regulation ... Date______________Per._______

... sun can lead to several types of skin cancer and to premature aging of the skin. The intensity of the ultraviolet rays actually alters and breaks certain sections of the DNA strand; thus mutations occur. To repair the structural damage done by the sun, a group of proteins, ultraviolet radiation A, B ...
CS691K Bioinformatics Kulp Lecture Notes #0 Molecular
CS691K Bioinformatics Kulp Lecture Notes #0 Molecular

... Humans: 23 pairs of chromosomes. Total ~3B “bases” (x2) DNA resides in nucleus in eukaryotes ...
Chapter 3 Practice Tes1
Chapter 3 Practice Tes1

Pre-AP Biology 2009
Pre-AP Biology 2009

... 12. Which part of the DNA molecule carries the genetic instructions that are unique to each individual: the sugarphosphate backbone or the nitrogen-containing bases? 13. In a sample of yeast DNA, 31.5% of the bases are adenine (A). Predict the approximate percentages of C, G, and T. C. DNA Replicati ...
Intermediate Inheritance or Incomplete Dominance
Intermediate Inheritance or Incomplete Dominance

... made up of 2 chains twisted around each other in a helical structure with pairs of nitrogenous bases projecting toward each other • Adenine will bond to thymine • Guanine will bond to Cytosine • Genes are considered to be segments of these molecules with the sequence of bases coding for the amino ac ...
Biology: Exploring Life Resource Pro
Biology: Exploring Life Resource Pro

... to answer this question. In this activity, you will model their experiment. • Examine the structure of the bacteriophage (also called a phage). Note that the phage is composed of only two types of molecules: protein and DNA. Click on the phage to begin. • The genetic material injected by the phage d ...
The Effects of Predictive Genetic Testing on the - Antioch Co-op
The Effects of Predictive Genetic Testing on the - Antioch Co-op

... of the double stranded DNA template into two single stranded molecules Annealing - The oligonucleotide primers anneal to or find their complementary sequences on the two single-stranded template strands of DNA. These act as primers for taq polymerase. All of this is done at 60℃ Extension - Taq polym ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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