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chapter08
chapter08

... Conjugation is the direct transfer of plasmids (or chromosomes) between bacteria It is a four-step process Contact between a donor cell (F+) and recipient cell (F-) is mediated by a sex pilus, a tubular structure The plasmid becomes mobilized by an enzyme that cleaves the plasmid One strand of the p ...
RECOMBINATION IN BACTERIA Transfer of Genetic Material in
RECOMBINATION IN BACTERIA Transfer of Genetic Material in

Review packet midterm 2016
Review packet midterm 2016

... 3. Draw and label the following parts of a chromosome: Chromatids and Centromere. ...
power point presentation
power point presentation

PRE-AP Stage 3 – Learning Plan
PRE-AP Stage 3 – Learning Plan

... on the family and their traits given to you. Assessment and Closing: Exit ticket will be the final product of the pedigree chart that was created. Opening: Warm-up to review Pedigrees and Karyotypes Guided Practice: Karyotype Lab-Which disorder do you have based on the karyotype. New Material: DNA f ...
Identify the goal of DNA replication Explain the role of DNA in
Identify the goal of DNA replication Explain the role of DNA in

... Synthesize a Identify the goal of DNA ...
Lecture 4 Linkage and Recombination
Lecture 4 Linkage and Recombination

... ‘A genetic map of the genes affecting adult height. Genetic linkage analysis was used for locating genes affecting stature. This method utilizes genetic markers known to show variation between individuals. The markers are evenly distributed across the entire genome and they are determined from DNA s ...
How We Know What Happened When
How We Know What Happened When

... *This number is for changes that affect the structure of the protein Lesson Plan about Molecular Clocks THEORY Introduction Every human cell has a "second" genome, found in the cell's energy-generating organelle, the mitochondrion. In fact, each mitochondrion has several copies of its own genome, a ...
Genetic Engineering
Genetic Engineering

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What does DNA do?
What does DNA do?

... ___ 3) Follow the base pair rule to fill in the missing base pairs for each strand. ___ 4) Compare your strands. Are they similar? What have you done? Where did this process take place in the cell? Why is this process important? They are identical copies of each other (mirror images). This process t ...
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Comprehension Questions

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... A. Different combinations of alleles are produced. B. Each allele from the parent cell forms a separate gamete. C. Each pair of genes undergoes crossing-over with different genes. D. The two genes are passed on to a daughter cell, resulting in new traits. 9. DNA and RNA are nucleic acids. A characte ...
Lect 4 JF 12
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... ‘A genetic map of the genes affecting adult height. Genetic linkage analysis was used for locating genes affecting stature. This method utilizes genetic markers known to show variation between individuals. The markers are evenly distributed across the entire genome and they are determined from DNA s ...
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Gene Technology

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Genome Sequencing Machine Learning for Big Data Seminar by Guided by

...  Genetic diseases often arise from simple changes to the coding region of a gene-altering the protein made by that gene. The disease arises because the protein does not work as it should do.  Some genomic conditions also affect coding regions. A translocation, for example, can end up fusing genes ...
APBiology 12
APBiology 12

... Bacterial plasmids are widely used as cloning vectors because they can easily be isolated from bacteria, manipulated to form recombinant plasmids by in vitro insertion of foreign DNA, and then reintroduced into bacterial cells. ...
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... RECOMBINANT DNA Recombinant DNA is used for the production of specific proteins  The first chemical produced by this mehtod was human insulin  The human gene is placed into a bacterium which can then use the genetic information to produce the human hormone.  The hormone is refined from the cultu ...
Effects of mutations
Effects of mutations

... Transcription and translation in eucaryotes • Similar to procaryotes except – AUG encodes for a different form of methionine – Transcription and translation are not simultaneous (since eucaryotes have a nucleus----transcription occurs in the nucleus, translation occurs ?) – Eucaryotes must splice o ...
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... • Most mutations are automatically repaired by the organism’s enzymes and therefore have no effect!!! • If not, the mutation can be passed on . . . . . . ...
Genetics of AHC - Alternating Hemiplegia of Childhood Foundation
Genetics of AHC - Alternating Hemiplegia of Childhood Foundation

...  New technology to look at all of the genes in a person’s cells ...
Biotechnology and Recombinant DNA I. Tools of Biotechnology
Biotechnology and Recombinant DNA I. Tools of Biotechnology

... This technology has lead to the development of a number of important tools for the study of life (e.g., Gene cloning (recombination), Hybridization analysis, Nucleotide sequence analysis, mutagenesis, gene expression, DNA fingerprinting). This technology relies on several basic principles • DNA as t ...
Blotting : Southern, Northern and Western techniques
Blotting : Southern, Northern and Western techniques

... broken or fragmented residues of agarose formed during banding and accumulated contaminants. ...
Appendix F - WordPress.com
Appendix F - WordPress.com

... design a forward primer and a reverse primer, each 10 bases long, to amplify a target sequence of the DNA that is at least 100 bp long. Write the sequence of the primers below, with their 3' and 5' ends indicated. Also indicate on the sequence above which strand they are complementary to (will annea ...
Nucleic acids - Haiku Learning
Nucleic acids - Haiku Learning

... together by all types of bonds, primarily through Rgroups Conjugated proteins also include elements and structures called prosthetic groups that are not amino acids (part of quaternary structure) ...
More on Genetics2013
More on Genetics2013

... Cystic Fibrosis(CF)—deletion of usually 3 bases ina gene for a protein called CFTR= Cystic Fibrosis Transmembrane Conductance Regulator….which normally allows Cl- ions to pass cell membrane-Phenylalanine is missing and protein folds improperly and is destroyed…causing multiple tissue malfunction-ser ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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