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... • Wild-type λ DNA contains several target sites for most of the commonly used restriction endonucleases and so is not itself suitable as a vector. • Derivatives of the wild-type phage have therefore been produced that either have a single target site at which foreign DNA can be inserted (insertional ...
Modeling Protein synthesis lab
Modeling Protein synthesis lab

... In a process called transcripaon which takes place in the nucleus of the cell, messenger RNA (mRNA) reads and copies the DNlt's nucleotide sequences in the form of a complementary RNA molecule. Then the mRNA carries this information in the form of a code to the ribosomes, where protein synthesis tak ...
Viruses
Viruses

... What do they need in order to reproduce? ...
Manipulating DNA - Emerald Meadow Stables
Manipulating DNA - Emerald Meadow Stables

Timeline Review - stephen fleenor
Timeline Review - stephen fleenor

... is an organelle that stores DNA, an endoplasmic reticulum, which makes new membranes, and lysosomes, which store and break down waste like a trash can. Just like they did with the chloroplasts, they also swallowed up ATP-producing bacteria and kept them in a membrane, which became an organelle calle ...
Unit 04 Part III - Githens Jaguars
Unit 04 Part III - Githens Jaguars

Recombinant DNA - University of Central Oklahoma
Recombinant DNA - University of Central Oklahoma

... and made pet fish that glow in the dark, when placed under a blacklight. They hit the shelves in 2004 to be sold as pets. ...
LHWHS Biology
LHWHS Biology

... ----Action of Restriction Enzymes----5. When combining human and bacteria DNA, a single stranded end of human DNA is matched up with a single stranded end of bactiria DNA . The ends that combine are called the..... ...
Anatomy and Physiology BIO 137
Anatomy and Physiology BIO 137

... Autosomal dominant means you only need to get the abnormal gene from one parent in order for you to inherit the disease. Autosomal recessive disorder means two copies of an abnormal gene must be present in order for the disease or trait to develop. ...
DNA Analysis
DNA Analysis

... 3.Markov Chains for DNA Sequences • Nucleotides are chained linearly one by one  local dependence between the bases and their neighbors • Markov chains offer computationally effective ways of expressing the various frequencies and local dependencies • Alphabet of bases = {A,T,C,G}  not uniformly ...
13-2 Manipulating DNA
13-2 Manipulating DNA

... assembled using laboratory machines known as DNA synthesizers. "Synthetic" sequences can then be joined to "natural" ones using enzymes that splice DNA together. The same enzymes make it possible to take a gene from ...
18 - cloudfront.net
18 - cloudfront.net

... assembled using laboratory machines known as DNA synthesizers. "Synthetic" sequences can then be joined to "natural" ones using enzymes that splice DNA together. The same enzymes make it possible to take a gene from ...
biology second semester study guide
biology second semester study guide

...  Summarize the events of DNA replication. ...
in Power-Point Format
in Power-Point Format

... from DNA-containing materials (Jeffreys et al., 1986) • Minisatellite DNA - sequence of bases repeated several times, also called DNA fingerprint – Individuals differ in repeats of basic sequence – – Difference large enough that 2 people have only remote chance of exactly same pattern • Other repeat ...
Cellular Metabolism
Cellular Metabolism

... Enzymes that control the reaction rates must also act in a specific sequence Enzymes are positioned in the exact sequence as that of the reaction ...
Ch. 16 – Control of Gene Expression Sample Questions
Ch. 16 – Control of Gene Expression Sample Questions

... A.Have their transcription occurring in the cytoplasm and translation in the nucleus. B.Have their transcription occurring in the nucleus and translation in the cytoplasm. C.Have only operons to assist in gene expression. D.Carry out protein synthesis only in the presence of the cAMP molecule. E.Use ...
a5_1_1-1_done
a5_1_1-1_done

O - morescience
O - morescience

... Genetically transformed bacteria that are: 1. Resistant (or shielded) from the effects of ampicillin 2. Marked with a Fluorescent Protein ...
Central Dogma of Biology - Marengo Community Middle School
Central Dogma of Biology - Marengo Community Middle School

... Transcription and translation are the two main processes linking gene to protein: an overview • Genes provide the instructions for making specific proteins. • The bridge between DNA and protein synthesis is RNA. • RNA is chemically similar to DNA, except that it contains ribose as its sugar and sub ...
Recombinant DNA Technology (Lecture 13)
Recombinant DNA Technology (Lecture 13)

... 2. erythropoietin (used to treat anemia by stimulating red blood cell production); 3. human growth hormone (somatotropin; used to treat growth deficiencies); 4. factor VIII (used to treat hemophilia); 5. alpha, beta, and gamma interferons (used against certain cancers and viral infections and as an ...
Slide 1
Slide 1

... would check whether their son, who was born in November, also has the mutation [for Parkinson’s], though he will not be able to donate his DNA in the usual way — putting saliva in small tubes, as 23andMe has promoted at celebrity-studded “spit parties.” “Babies can’t spit into a tube,” Mr. Brin said ...
genetic ppt melanie - IB
genetic ppt melanie - IB

... allowing annealing of the primers to the single-stranded DNA template. Stable DNADNA hydrogen bonds are only formed when the primer sequence very closely matches the template sequence. The polymerase binds to the primer-template hybrid and begins DNA formation. Extension/elongation step: commonly a ...
Functional Protein detection for DNA Mismatch Repair: A Novel Nano
Functional Protein detection for DNA Mismatch Repair: A Novel Nano

... Cancer currently stands as the second-leading cause of death worldwide. Studies reveal colorectal cancer (CRC) to be the 4th leading cause of mortality due to cancer. It is estimated that about 30% of CRC cases are hereditary, of which 5% are attributed by known syndromes, particularly Lynch Syndrom ...
Protein Synthesis
Protein Synthesis

... amino acids into a polypeptide chain (protein) Performed in nucleus by mRNA mRNA “reads” single DNA strand and forms the complementary copy ...
Molecular motors: DNA takes control
Molecular motors: DNA takes control

... which are important in many transport and trafficking processes in cells. Furthermore, DNA self-assembly techniques such as DNA origami5 have been used to create larger and more complex multiple motor ensembles. In particular, DNA origami has been used to connect different types of motor, which coul ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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