• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Chapter 9
Chapter 9

Two powerful transgenic techniques Addition of genes by nuclear
Two powerful transgenic techniques Addition of genes by nuclear

More on Genetics
More on Genetics

... Mutations are a source of genetic variation DNA extraction-add chemicals that cause DNA to uncoil from histones and burst out of nucleus ________________________________making changes in the DNA code of an organism _________________________________are used to cut DNA into fragments and gel electroph ...
CHNOPS- Simulating Protein Synthesis
CHNOPS- Simulating Protein Synthesis

... tRNAs arrive in turn and give up the amino acids they carry to the growing polypeptide chain. The process by which the information from DNA is transferred into the language of proteins is known as translation. Transcription and translation together comprise the process called protein synthesis or ge ...
2.5.15 Summary - Intermediate School Biology
2.5.15 Summary - Intermediate School Biology

... Chromosome: A thread-like structure found in the nuclei of dividing cells, and composed of a super-coiled arrangement of DNA and protein Chromosome structurThread of DNAWra Chromosome structure • Thread of DNA ...
Chapter 8 Nucleotides and Nucleic acids
Chapter 8 Nucleotides and Nucleic acids

... Can appear in sites of initiation or regulation of DNA replication recombination or strand separation But again in vivo implication unclear Depend on additional base pairing See figure 8-20 both 3-ple and 4-ple So can fuse 3 or 4 strands together These non-Watson-Crick base pairing called Hoogsteen ...
Gene Expression—Transcription
Gene Expression—Transcription

... In eukaryotes the enzyme RNA polymerase joins with several transcription factor proteins at the promoter, which is a special sequence of base pairs on the DNA template strand that signals the beginning of a gene. The transcription factor proteins, along with the RNA polymerase, is called the transcr ...
Chapter 11 - BickfordBiology
Chapter 11 - BickfordBiology

... • A code is used to convert the language of mRNA into proteins • There are 20 different amino acids; however, mRNA contains only 4 types of bases (A-U-G-C) – A group of three nucleotides codes for one ...
Recombinant DNA Answer Key
Recombinant DNA Answer Key

... Copying DNA Genetic engineers can transfer a gene from one organism to another to achieve a goal, but first, individual genes must be identified and separated from DNA. The original method (used by Douglas Prasher) involved several steps: ▶ Determine the amino acid sequence in a protein. ▶ Predict t ...
DNA Profiling - Mrs. Blackmon`s Science Blackboard
DNA Profiling - Mrs. Blackmon`s Science Blackboard

... 1. Cells are isolated from biological evidence such as blood, saliva, urine, semen, and hair 2. The cells are disrupted to release the DNA from proteins and other cell components 3. The DNA can be extracted from the cell nucleus ...
The wrong file for Lecture 8 was posted on the website. I`ve sent the
The wrong file for Lecture 8 was posted on the website. I`ve sent the

... There are a number of different moderately repetitive sequences. Minisatellites are variable number tandem repeats (VNTPs). The sequences are variable in length (10-100 bp), but within a repeat sequence, the individual sequences will be the same. VNTPs create regions of 1000-5000 bp in length ...
Adobe Acrobat Document
Adobe Acrobat Document

... single DNA strand and forms the complementary copy. How transcription works enzyme 1. DNA strand splits, with the help of an _____________ called DNA helicase _______________. *This exposes the active strand ...
DNA replication notes
DNA replication notes

... binds and attaches its amino acid to the first one, with a peptide bond. 2. This continues down the mRNA strand, until the ribosome reaches one of the ...
Mechanism of Surface Stress due to DNA strands on Gold
Mechanism of Surface Stress due to DNA strands on Gold

... • Usually about 20 nucleotides in length • Designed to flank the region to be amplified ...
Marshall Nirenberg and the discovery of the Genetic Code
Marshall Nirenberg and the discovery of the Genetic Code

... Protein biosynthesis • It was known that the synthesis of proteins occurred on ribosomes, microgranules that are present in the cytoplasm, and not in the nucleus • Also, degradation of DNA by an enzyme called DNAase did not stop the synthesis of proteins • So it was concluded that DNA could not dir ...
MATCH
MATCH

... mRNA (mature form) a) ______________________ translated into an amino acid sequence b) ______________________ used in the building of ribosomes c) ______________________ carry specific amino acids to growing polypeptide chain d) ______________________ complex with proteins to form snurps and the spl ...
Mutations (1 of 2)
Mutations (1 of 2)

... Mutation is a change in DNA, the hereditary material of life. An organism’s DNA affects how it looks, how it behaves, and its physiology—all aspects of its life. So a change in an organism’s DNA can cause changes in all aspects of its life. Mutations are random. Mutations can be beneficial, neutral, ...
DNA metabolism
DNA metabolism

... genetic defect causes XP, xeroderma pigmentosa, these individuals are extremely sensitive to sunlight and quickly develop sunlight-induced skin cancer Mismatch repair Hereditary nonpolyposis colon cancer (HNPCC) linked to defects in these genes Recombinational repair Recombination - linear sequence ...
No Slide Title
No Slide Title

... genetic defect causes XP, xeroderma pigmentosa, these individuals are extremely sensitive to sunlight and quickly develop sunlight-induced skin cancer Mismatch repair Hereditary nonpolyposis colon cancer (HNPCC) linked to defects in these genes Recombinational repair Recombination - linear sequence ...
Biology Final Exam Review
Biology Final Exam Review

... three of the cells die, leaving only one viable cell. ...
Troubling and Terrific Technology
Troubling and Terrific Technology

... (the place where it will cut. Restriction fragments - when DNA is exposed to a restriction enzyme, the enzyme cuts it in the same place every time. This leaves the same fragments of DNA every time it is exposed to the restriction enzyme Sticky ends - restriction fragments can be staggered, leaving s ...
Response to Environment_01_Model Checklist
Response to Environment_01_Model Checklist

... 4) In the cytoplasm, ribosomes then translate all of these different mRNAs into all of the different proteins that can then go on to do their jobs in the cell. Show the details of a generic example either in your story or off to the side. [Not only does the cell have to double all of its proteins an ...
Supplementary Material Genomic DNA isolation and bisulfite
Supplementary Material Genomic DNA isolation and bisulfite

... media was then removed from the inserts and replaced with fresh 0.5 mLs of serum-free containing resuspended cells (104 cells). To the lower wells of the plate, 0.75 mLs of Complete Tu medium containing 2% FBS was added. WM1552C/375 cells were additionally transfected with the mIRIDIAN Hairpin Inhib ...
DNA Technology and its Applications
DNA Technology and its Applications

... Using the technology of recombinant DNA, we are able to introduce specific genes from one organism into another. ...
A 1
A 1

... Characters are controlled by pairs of genes which separate during the formation of the reproductive ...
< 1 ... 504 505 506 507 508 509 510 511 512 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report