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Chapter 25: Molecular Basis of Inheritance
Chapter 25: Molecular Basis of Inheritance

... complementary positions are joined by DNA polymerase. The process is semiconservative because each new double helix is composed of an old strand of nucleotides from the parent molecule and one newly-formed strand. Some cancer treatments are aimed at stopping DNA replication in rapidlydividing cancer ...
Excretion is the process in which _____ is (are) removed from the
Excretion is the process in which _____ is (are) removed from the

... specifically recognizes a foreign antigen. Antibiotic: a chemical substance that can kill or inhibit the growth of a microorganism. Antigen: a molecule, usually a protein or polysaccharide (sugar), that induces the production of specific antibodies against itself. Molecules on the surfaces of viruse ...
Lecture #21 - Faculty Web Sites at the University of Virginia
Lecture #21 - Faculty Web Sites at the University of Virginia

... strains emerge rapidly Therefore, virus production requires a given cell to be simultaneously infected by numerous viruses so mutants can complement each other to generate viral progeny ...
Genetic Engineering - fhs-bio
Genetic Engineering - fhs-bio

... In November 2001, scientists from Advanced Cell Technologies (ACT), a biotechnology company in Massachusetts, announced that they had cloned the first human embryos for the purpose of advancing therapeutic research. To do this, they collected eggs from women's ovaries and then removed the genetic ma ...
Chapter 10 – DNA Replication
Chapter 10 – DNA Replication

... – Starting point of replication • A-T rich regions ...
File - Personal FSU Notes
File - Personal FSU Notes

... • Nucleosomes are condensed several times to form the intact chromatids • The DNA in a human cell would be ~2 meters long if it were “unwound”. • The nucleus is 5-10 mm (5 x 10-6 m) in diameter • Eukaryotic chromosomes are complexed into a nucleoprotein structure called chromatin. • Chromatin is bou ...
Forensic DNA Testing Terminology ABI 310 Genetic Analyzer – a
Forensic DNA Testing Terminology ABI 310 Genetic Analyzer – a

... of their size. Adenine – a purine base; one of the four molecules containing nitrogen present in the nucleic acids DNA and RNA; designated by letter A. Allele – one of two or more alternative forms of a gene. Allele Frequency – the proportion of a particular allele among the chromosomes carried by i ...
Neanderthals get in on the action - Max
Neanderthals get in on the action - Max

... try to prevent contamination at the excavation site and during their subsequent molecular-biological studies  (Fig.  A) , or – if that is not possible or no longer possible – to identify the contamination when analysing the sequencing data. In doing so, they also avail of the fact that, post mortem, ...
DNA & Protein Synthesis
DNA & Protein Synthesis

... 5'3' direction forming an okazaki fragment. Until it reaches the next fragment. • The two fragments are joined by the enzyme DNA ligase • Two, new, identical DNA strands are now formed ...
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A1983QN93000002

... A phenomenon by which genes in bacteria are exchanged and recombined has been discovered. The temperate bacteriophage, PLT-22, during growth has its genome replaced by bacterial genes. Each particle carries about one percent of the bacterial genome. These genes are carried to a new host cell in phag ...
Name: ____________ Pd.: ______ Date: Cells cannot make
Name: ____________ Pd.: ______ Date: Cells cannot make

... 1. Cells cannot make proteins directly from DNA. They must convert the DNA into an intermediate molecule called RNA, or ribonucleic acid. That conversion process is called __________________________________________. 2. The __________________ _________________ of molecular biology states that informa ...
Chapter 01 Lecture PowerPoint
Chapter 01 Lecture PowerPoint

... • Genotype is the combination of alleles found in an organism • Phenotype is the visible expression of the genotype – Wild-type phenotype is the most common or generally accepted standard – Mutant alleles are usually recessive ...
Review 1 - LFHS AP Biology
Review 1 - LFHS AP Biology

... sister in the middle generation is actually heterozygous? ...
The Molecular - MolGen | RuG
The Molecular - MolGen | RuG

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Biosafety and recombinant DNA technology

... are propagated in cell lines that complement the defect. • Stocks of such vectors may be contaminated with replication-competent viruses, generated by rare spontaneous recombination events in the propagating cell lines, or may derive from insufficient purification. • These vectors should be handled ...
Origin of Sexual Reproduction
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Genomics - University of Missouri
Genomics - University of Missouri

... The study of the entire DNA complement of an individual. The term genome refers to all of the DNA contained in one copy of the chromosomes of an organism. It contains both coding (genes) and non-coding DNA sequences. ...
Strawberry DNA extraction lab activity
Strawberry DNA extraction lab activity

... interestingly, strawberries have enormous genomes. They are octoploid, which means they have eight of each type of chromosome (which equals abundant DNA). ...
Microbial Metabolism
Microbial Metabolism

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Chapter 9. Pg 189 DNA: The Genetic Material
Chapter 9. Pg 189 DNA: The Genetic Material

... b. In a nucleic-acid chain, a subunit that consists of a sugar, a phosphate, and a nitrogenous base. c. A characteristic of nucleic acids in which the sequence of bases on one strand is paired to the sequence of bases on the other. d. The spiral staircase structure characteristic of the DNA molecule ...
SPMS Unit 3.1 DNA Profiling File
SPMS Unit 3.1 DNA Profiling File

... a. What do they have in common? b. Describe how they differ? c. How are they each used in forensics? Elaborate on STRs used in DNA analysis. a. STR is an abbreviation for which words? b. Where do you find STRs? c. How many bases are usually found within an STR? d. List several examples of STRs. e. I ...
Recitation 4 - MIT OpenCourseWare
Recitation 4 - MIT OpenCourseWare

... Meiosis has two divisions; meiosis 1 looks different from mitosis, but meiosis II looks similar to mitosis. The result of meiosis is the production of haploid gamete cells such as eggs or sperm, each of which has half the number of chromosomes as a diploid somatic cell. Recombination and crossing ov ...
cancer epigenetics - Experimental oncology
cancer epigenetics - Experimental oncology

Ch 15 Help - Practice Regents Answer Key
Ch 15 Help - Practice Regents Answer Key

... B) half of the genetic information of the new animals is the same as that of the original pets C) the new animals have mutations not found in the original pets D) the new animals have the same genetic information as the original pets 8. A technique used to produce new plants is represented in the di ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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