• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
gene_expression_info
gene_expression_info

... • Polypeptides are chains of amino acid residues joined by peptide bonds. • Proteins are large polypeptides. • There are 20 different aa and their sequence determines the structure and function of the protein. • The sequence of bases in a DNA molecule determines the sequence of aa. • A gene is a len ...
Principios de Biología Molecular
Principios de Biología Molecular

... • Transcription is highly regulated. Most DNA is in a dense form where it cannot be transcribed. • To begin transcription requires a promoter, a small specific sequence of DNA to which polymerase can bind (~40 base pairs “upstream” of gene) • Finding these promoter regions is a partially solved prob ...
Slide 1
Slide 1

... • Plasmids can transfer genes and act as carriers for introducing DNA from other bacteria or from eukaryotic cells • E. coli cell membrane is weakened using • E. coli cells are then “ • Sterile technique must be used ...
Do-It-Yourself Strawberry DNA
Do-It-Yourself Strawberry DNA

... break open the cell. This can be done by grinding a piece of tissue in a blender. After the cells have broken open, a salt solution such as NaCl and a detergent solution containing the compound SDS (sodiumdodecyl sulfate) is added. These solutions break down and emulsify the fat & proteins that make ...
Lesson Overview
Lesson Overview

... replicated, because each base on one strand pairs with only one base on the opposite strand. Each strand of the double helix has all the information needed to reconstruct the other half by the mechanism of base pairing. Because each strand can be used to make the other strand, the strands are said t ...
Unit 5 Test Review 14-15
Unit 5 Test Review 14-15

... What are the different types of RNA and what is their function: ...
Biological vocabulary glossary, part 1
Biological vocabulary glossary, part 1

... The bases that occur in DNA are C​ytosine​ (C),​ guanine​ (G),​ adenine​ (A), or​ thymine​ (T). Instead of Thymine, Uracil (U) is used in RNA; the other bases stay the same. A​ phosphate group​ links together sugar backbone and bases. Together, these three elements create a ​nucleotide​ - the basic ...
Lesson Plan
Lesson Plan

... amino acids (elaborate on this). Explain the structure of proteins- a basic structure of amino acids. From 20 amino acids coded in the RNA, we get an infinite number of different proteins. 7. How can we manipulate the knowledge we have about DNA and traits? With the help of genetic engineering which ...
7.1 DNA Introduction
7.1 DNA Introduction

CHP13ABIOH - willisworldbio
CHP13ABIOH - willisworldbio

... sheep which produce a ______ that helps lungs inflate and function properly. ...
how to read a pedigree - Doral Academy Preparatory
how to read a pedigree - Doral Academy Preparatory

... cuts that have single stranded ends Attract corresponding base pairs Made by special restriction (cutting) enzymes GGCCATTAC Stick together TACCGG CCGC TAATGATGGC ...
Genetics Test 2
Genetics Test 2

... called a carrier (Cc) of the disease. If the mother is a carrier of the disease and the father is homozygous dominant, what are the chances that their child will be a carrier of cystic fibrosis? ...
DNA Fingerprinting
DNA Fingerprinting

... – They do not form any tissues, they just grow as individual stem cells – Researchers can grow millions and millions of these in the lab to perform studies that may someday save lives and cure diseases ...
Jet-swirl nozzle design for producing nanoscale polymer
Jet-swirl nozzle design for producing nanoscale polymer

... All living things are composed of either: (a) prokaryotic cells - those lacking a nucleus such as bacteria where the genetic information is found in nucleoid matter (b) eukaryotic cells - complex cells having a nucleus similar to the animal cell shown here. ...
What is a pedigree? - River Mill Academy
What is a pedigree? - River Mill Academy

... Recombinant DNA Uses: Grow human hormones in bacteria cultures  Artificial sweeteners using bacteria to make amino acids  Study human diseases by inserting human DNA into mice ...
ppt link
ppt link

... Prepare DNA for complementary base pairing Proteins connect the correct sequences of nucleotides into a continuous new strand of DNA ...
Heidi Sleister
Heidi Sleister

... Chromosomes are made of DNA and are found in the nucleus of a cell ...
Oral topics from the basic requirements
Oral topics from the basic requirements

... Sex-linked inheritance Linkage groups, genetic maps family trees Lac operon Promoters, enhancers, silencers transcription factors Cell type-specific and induced gene expression The structure of chromatin Epigenetics in general Epigenetics: methylation and acetylation of the histone Epigenetics: DNA ...
E1. A. Cytogenetic mapping B. Linkage mapping C. Physical
E1. A. Cytogenetic mapping B. Linkage mapping C. Physical

... E5. The term fixing refers to procedures that chemically freeze cells and prevent degradation. After fixation has occurred, the contents within the cells do not change their morphology. In a sense, they are frozen in place. For a FISH experiment, this keeps all the chromosomes within one cell in the ...
Genes and How They Work
Genes and How They Work

... activator that is turned OFF in the presence of a substrate and a promotor that is turned ON in the presence of a product.  Define a series of gene regulators that would turn a ‘cascade’ on and then off in series  Define, promotor, activator and operator ...
Heredity,Gene Expression, and the
Heredity,Gene Expression, and the

... ● Many code for growth factors & other proteins that stimulate or regulate cell cycle. ● Tumor suppressing genes: when normal, slow & control cell growth & division. DNA Mutation of these genes may result in loss of control over cell cycle. Multiple mutations required for a full-fledged cancer cell: ...
Document
Document

... E5. The term fixing refers to procedures that chemically freeze cells and prevent degradation. After fixation has occurred, the contents within the cells do not change their morphology. In a sense, they are frozen in place. For a FISH experiment, this keeps all the chromosomes within one cell in the ...
8000 - International Commission on Missing Persons
8000 - International Commission on Missing Persons

... The total number of Srebrenica-related sites where human remains have been recoverd is 430. This includes 94 graves, and 336 surface sites. Over 17,000 sets of human remains (bodies and body parts) related to Srebrenica 1995 have been examined in mortuaries by pathologists and anthropologists. ...
DNA replication limits…
DNA replication limits…

... true of so-called spontaneous mutations. "Spontaneous" refers to the fact that the changes occur in the absence of chemical, radiation, or other environmental damage. Rather, they are usually caused by normal chemical reactions that go on in cells, such as hydrolysis. These types of errors include d ...
Biology Final Exam Multiple Choice Identify the choice that best
Biology Final Exam Multiple Choice Identify the choice that best

... a. without an X chromosome. b. with one X chromosome. c. with four X chromosomes. d. with three X chromosomes. ...
< 1 ... 500 501 502 503 504 505 506 507 508 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report