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Histone Methylation
Histone Methylation

... Transcriptional - These mechanisms prevent transcription. Posttranscriptional - These mechanisms control or regulate mRNA after it has been produced. Translational - These mechanisms prevent translation. They often involve protein factors needed for translation. Posttranslational - These mechanism ...
DNA (Gene) Mutations
DNA (Gene) Mutations

... Enzymes proofread the DNA and replace incorrect nucleotides with correct nucleotides. The greater the exposure to a mutagen such as UV light, the greater the chance that a mistake will not be corrected. ...
Unit 5 Molecular Genetics Objectives
Unit 5 Molecular Genetics Objectives

... 3 Inducers and repressors are small molecules that interact with regulatory proteins and/or regulatory sequences. 4 Regulatory proteins inhibit gene expression by binding to DNA and blocking transcription (negative control). 5 Regulatory proteins stimulate gene expression by binding to DNA and stimu ...
Distinguishing endogenous versus exogenous DNA
Distinguishing endogenous versus exogenous DNA

... In the late 1990s it was reported that human DNA existed on the Shroud of Turin, and although in a generally degraded state, certain regions were sufficiently intact to clone and sequence three genes from bloodstained fibers: human betaglobin, amelogenin X and amelogenin Y. An unknown variable in su ...
Final Examination
Final Examination

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Student Name: Teacher
Student Name: Teacher

... Break down the nuclear membrane of cells, releasing DNA. Isolate DNA from proteins and sugars in the solution. Protect nucleic acids from enzymes used to destroy proteins. Sterilize the material from which DNA is to be extracted. ...
Recombination is the principal source of variation in asexually
Recombination is the principal source of variation in asexually

... 60. Triploid watermelons are derived from crosses of tetraploid x diploid parents. a) T b) F 61. The cultivated banana is triploid and sterile. Which of the following is the most likely source of genetic variation in this species? a) Mutation b) Recombination 62. The fact that there can be up to fo ...
Evolution - Museums Victoria
Evolution - Museums Victoria

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Advanced Environmental Biotechnology II
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HG06_geneexpression
HG06_geneexpression

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DNA Fingerprinting

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2013 Training Power Point
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Sections 5.3-5.5 - BridgesToLiteracy.com
Sections 5.3-5.5 - BridgesToLiteracy.com

... they serve as a fuel and building material. they both include sugars and the polymers of sugar. -carbohydrates will be seen on CH.6,7,9 and 41 -in Ch. 41, when a person eats too many carbohydrates, the body increased its rate of carbohydrate oxidation (a loss of electrons from a substance involved i ...
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... the sequence of one strand given the other: s→s’ →s bar / AGACGT-TGCAGAACGTCT (always 5-3) replicate: It is precisely this mechanism that allow DNA in a cell to replicate, therefore allowing an organism that starts its life as one cell to grow into billions of other cells, each one carrying copies o ...
Allele: alternative form of a gene, e
Allele: alternative form of a gene, e

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File - EUREKA! Science
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GENERAL ZOOLOGY LECTURE EXAM 2
GENERAL ZOOLOGY LECTURE EXAM 2

... e. An organism that is homozygous for many recessive traits is at a disadvantage. 12. If an organism's 2n number is 12, how many chromosomes will be present in each daughter cell at the end of meiosis II? a. 2 b. 6 c. 12 d. 24 e. 48 13. Can Mendel’s law of independent assortment be expected to hold ...
DNA Mutations
DNA Mutations

...  Genes are removed from the chromosome  This can occur anywhere on the chromosome  Can cause disorders such as Cri Du Chat, or “cry of the cat” syndrome. ...
Predicted Existence of Messenger RNA: The Operon Model Until
Predicted Existence of Messenger RNA: The Operon Model Until

... If all cells have the same DNA content, and the DNA of a cell specifies its activities (what enzymes it makes) and characteristics (what effect these enzymes have), why aren't all cells the same? We know that there are different cell types in our bodies, and that the activities of these cells change ...
Recombinants and Linkage Maps
Recombinants and Linkage Maps

... a linkage map for a particular chromosome are obtained from experimental crosses, such as the cross depicted in Figure 15.6. The distances between genes are expressed as map units (centimorgans), with one map unit equivalent to a 1% recombination frequency. Genes are arranged on the chromosome in th ...
Alveoli - greinerudsd
Alveoli - greinerudsd

... Law of independent assortment: The inheritance of one gene does not influence the inheritance of another. In meiosis, the chromosomes line up randomly on the equator to be separated. If your parents are heterozygous for any traits, this leads to lots of possibilities! What are homologous chromosomes ...
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... In 1968, Dr. Werner Arber at the University of Basel, Switzerland and Dr. Hamilton Smith at the Johns Hopkins University, Baltimore, discovered a group of enzymes in bacteria, which when added to any DNA will result in the breakage [hydrolysis] of the sugarphosphate bond between certain specific nuc ...
Gene Regulation Summary Slide Questions with
Gene Regulation Summary Slide Questions with

... 2. What is the difference between negative and positive regulation? Negative regulation is done by a repressor to impede the gene; positive regulation is done by an activator which enhances the pol/promoter interaction. A corepressor enhances the binding of a repressor, so that it repressors further ...
Lecture 8. DNA AND THE LANGUAGE OF LIFE
Lecture 8. DNA AND THE LANGUAGE OF LIFE

... TO MAKE USEFUL PRODUCTS – The plasmids engage in gene transfer that can spread traits that aid the bacterial cells to survive, such as antibiotic resistance. – The plasmid can be used for good purposes such as gene cloning. • The plasmid is removed from the bacterial cell. • A desired gene from any ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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