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What unites these phenomena?
What unites these phenomena?

... Dnmt1 and associated proteins scan newly replicated DNA for hemimethylated sites and methylate the CpG’s on the newly synthesized strands ...
Cloning The Insulin Gene
Cloning The Insulin Gene

Biology Fall 2013 Final Review
Biology Fall 2013 Final Review

... 35. Name the nitrogen bases found in DNA and what they bond to. ...
Introduction to your genome
Introduction to your genome

... 1. Inheritance is determined by “units” (now called genes) 2. An individual inherits one such unit from each parent for each trait 3. A trait my “skip” a generation ...
LG and SC 2017 10 genetics
LG and SC 2017 10 genetics

... LG3 I can describe mutations as changes in DNA or chromosomes and outline the factors that contribute to causing mutations SC19 I can define mutation SC20 I can identify at least 2 factors that cause mutations and the effect these may have on living things LG4 I can understand how the theory of evol ...
Blueprint for life - Siemens Science Day
Blueprint for life - Siemens Science Day

... Tell students that it is a greeting and ask them if they can figure out what it says. Lead students to recognize that you have written the word “hello” in code. Each letter is represented as a number, with a = 1, b = 2, c = 3, and so on. Ask students if they know that the cells in their bodies conta ...
Biology and the Body Final Review 2014
Biology and the Body Final Review 2014

... enough? ...
25Ch03nucleicacids2008
25Ch03nucleicacids2008

... AP Biology ...
recombinant dna
recombinant dna

... example, DNA micro arrays containing sequences from all the genes of E. coli are available commercially), or a DNA array may contain sequences from numerous species. In any case, single strands of fluorescently labeled DNA in a sample washed over an array adhere only to locations on the array where ...
DNA
DNA

...  Then the membrane is placed over standard X-ray film where the radiation emitted from the P-32 gradually exposes the film and shows the DNA bands. This process takes about 10 weeks to complete. ...
Basic Science Notes
Basic Science Notes

Knackstedt, K.A., H.B. Thorpe, C.R. Santangelo, M.A. Balinski, and R
Knackstedt, K.A., H.B. Thorpe, C.R. Santangelo, M.A. Balinski, and R

... genetic analysis, and lends itself to collaborative work within groups, class presentations and formal lab reports. The availability of multiple inbred strains for class use will increase the likelihood that some students will select two strains with different mean values for the assayed trait. The ...
Two Epigenetic Mechanisms
Two Epigenetic Mechanisms

... Same genes, different phenotypes NOVA’s A Tale of Two Mice: Chapter 1 ...
DNA Replication Reading - Lesley Anderson`s Digital Portfolio
DNA Replication Reading - Lesley Anderson`s Digital Portfolio

... the journal article announcing their discovery with this sentence: “It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.” Recall that the bases that connect the strands of DNA will pair only in one way, ...
ch03dwcr
ch03dwcr

... • DNA helices unwind from nucleosomes • Each nucleotide strand serves as a template for building a new complementary strand • DNA polymerase makes the complementary strands • End result: two DNA molecules formed from the original ...
Endosymbiotic Hypothesis (Endosymbiosis)
Endosymbiotic Hypothesis (Endosymbiosis)

... Also, add the 6 pieces of evidence that support this. 1. Mitochondria and Chloroplast replicate (make copies of themselves) like bacteria. 2. Membrane Similarity with bacteria. 3. Mitochondria and Chloroplasts have their own DNA 4. Ribosome size is similar to bacteria. 5. Size of cells is similar to ...
dna & cell division
dna & cell division

...  DNA Polymerase proofreads nucleotides as they are added ...
Genetic mechanisms
Genetic mechanisms

... not use formyl-methionine. Eucaryotic mRNA encodes a single protein, unlike bacterial mRNA which encodes many (operon). Eucaryotic DNA contains introns – intervening sequences of noncoding DNAwhich have to be spliced out of the final mRNA transcript. ...
Mitosis Meiosis
Mitosis Meiosis

... 9. Mitosis has only _____ division step, while meiosis has _____ divisions. 10. The result of mitosis is _____ cells each with genetic material _____ to the parent cell. Meiosis results in _____ gametes, each with only _____ the genetic material of the original cell. ...
DNA WebQuest
DNA WebQuest

... Click Next and then click on each organism until you identify the one that belongs to chromosome #1; continue playing the game with the other two chromosomes, filling in the chart below. Be careful, other teams may get different results. Chromosome # ...
View PDF
View PDF

... 15. Explain what DNA polymerase is by breaking the word into its parts. _______________________________________________________________ 16. Write a short analogy to explain what replication is. _______________________________________________________________ ...
week 13_genetic information
week 13_genetic information

... • They observed that during replication, each parental strands serves as a template for DNA synthesis. • After new strand is formed, it is hydrogen bonded to its parental strand. • Each of the double helix contains one parent DNA strand and one newly synthesized daughter strand. ...
Metzenberg, R.L. and J. Grotelueschen
Metzenberg, R.L. and J. Grotelueschen

... 1985. 82:2067-2071; Metzenberg and Grotelueschen, 1987. Fungal Genetics Newsl. 34:3944). The following data include the previous scorings of two crosses from the 1987 article and contains new data on the same two crosses from our own lab, and from others. As noted in the 1987 article, 38 segregants ...
The Big Picture: an outline of the concepts covered to date
The Big Picture: an outline of the concepts covered to date

Nutrigenomics and nutrigenetics – are they the keys for healthy
Nutrigenomics and nutrigenetics – are they the keys for healthy

... Methylation (folate) ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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