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The Big Picture: an outline of the concepts covered to date
The Big Picture: an outline of the concepts covered to date

Nutrigenomics and nutrigenetics – are they the keys for healthy
Nutrigenomics and nutrigenetics – are they the keys for healthy

... Methylation (folate) ...
F - cell
F - cell

... Ammonium (NH4+) is used as the sole N source by most microorganisms. Ammonium could be produced from N2 by nitrogen fixation, or from reduction of nitrate and nitrite. ...
File - Mrs. Lucier and Mrs. Magagna Life Science Class
File - Mrs. Lucier and Mrs. Magagna Life Science Class

... Fill in the blank with words found below. 1. ______________________ is a process in which defective genes are replaced ...
What is DNA sequencing
What is DNA sequencing

... Both the Maxam-Gilbert and Sanger-Coulson methods can only produce about 400 bases of sequence at a time. Most genes are larger than this. To sequence a large DNA molecule it is cut up (using two or more different restriction enzymes) into different fragments and each fragment is sequenced in turn 1 ...
Genetics Vocabulary Note-Taking Chart
Genetics Vocabulary Note-Taking Chart

... alleles for a trait occupy the same position on homologous chromosomes and thus govern the same trait. An inherited trait which is present even when inherited only from one parent. the form of the gene that shows up only when inherited from both parents. Requires both alleles to show the likelihood ...
HIV GENOTYPE ASSAY
HIV GENOTYPE ASSAY

... entire region Protease (codon 1-99) and two-thirds RT region (1335). Big Dye Terminator chemistry is used to permit a resolution of 600 bases on the 3100 Genetic Analyzer  Sequence Purification-removes unincorporated Big terminators from samples so they do not interfere with sample sequencing & ana ...
DNA Technology - De Anza College
DNA Technology - De Anza College

... But, what new capability does E. coli have? Produces a ‘new’ protein From that gene segment ...
Lesson 4: Genetic Engineering Worksheet
Lesson 4: Genetic Engineering Worksheet

... Two major techniques in genetic engineering; 1) genes are engineered into organisms such as microbes (bacteria) for mass-production and 2) insertion of select genes into organisms to improve their genotype. For plants and animals we use tissue culturing to increase the number in bulk quantity. 2. Id ...
Document
Document

... Examine how interactions among the different molecules in the cell cause distinct stages of the cell cycle which can also be influenced by other signaling molecules. Explain the role of mitosis in the formation of new cells and its importance in maintaining chromosome number during asexual reproduct ...
JSReviewExam#4
JSReviewExam#4

...  Why are there differences between large and small genomes? polyploidy and amount of repetitive DNA  Definition of polyploidy  Humans have 2 sets of 23 chromosomes/total 46  Transposable elements: "selfish DNA", inserts itself into new positions in genome; about 45% of human DNA  Bacterial geno ...
Preparation of SCRATCHY Hybrid Protein Libraries
Preparation of SCRATCHY Hybrid Protein Libraries

AIMS Review Packet
AIMS Review Packet

... 53) What is Incomplete dominance? Give an example When both alleles are present there is a blending of the phenotype. Example: White flower or Red flowers: Rr=pink flowers ...
14-1 - Fort Bend ISD
14-1 - Fort Bend ISD

... **Chromosome 22 contains as many as 545 different genes, some of which are important for health. One allele may cause a form of leukemia Another may cause neurofibromatosis Long stretches of DNA may be repetitive and not code for proteins -unstable- rearrangements can occur. ...
D.N.A. activity
D.N.A. activity

... a) the length of the total DNA, uncoiled and laid end-to-end, within one human somatic cell. b) The length of the entire amount of DNA in their body (again, assume uncoiled and laid out). c) The closest astronomical object to that distance. d) OPTIONAL: The total number of trips to the sun and back ...
Genetic Modification Regulations and Procedures
Genetic Modification Regulations and Procedures

投影片 1
投影片 1

... Some viruses, nonlytic association with host cell (not kill) is called temperate phages Prophage: integrated into the host cell chromosomes rather than being replicated Lysogeny: Instead of destroying host to produce virus progeny, the viral genome remains within the host cell and replicates with th ...
Exploring DNA Structures
Exploring DNA Structures

... Background Information: DNA is the basic material that contains the information that is responsible for the way all living organisms physically look and instruction on how to carry out the activities of the cell. We are going to explore the different parts of DNA. READ THIS BEFORE MOVING ON: Before ...
DNA Identity
DNA Identity

... DNA can be removed from cells and collected using a process called DNA extraction. The process must first remove DNA from inside cell and nuclear membranes. Once these membranes are destroyed, however, DNA tends to bind to proteins that are also freed and floating in the mixture. A solution of deter ...
Solutions  - MIT OpenCourseWare
Solutions - MIT OpenCourseWare

... Besides a possible difference in base structure, what is the major structural difference between ribo-and deoxyribonucleosides? Solution Deoxyribonucleosides have a hydrogen instead of a hydroxyl group at the C2 position of the sugar. Problem #7 Show that you understand hoe the structural components ...
Document
Document

... nucleotide. Using this technology the presence of one genomic or cDNA sequence in 1,00,000 or more sequences can be screened in a single hybridization. The property of complementary nucleic acid sequences is to specifically pair with each other by forming hydrogen bonds between complementary nucleot ...
Slide 1
Slide 1

... Telomeres are specialized DNA sequences that cap the ends of linear chromosomes and provide protection against gene erosion at cell divisions, chromosomal non-homologous end-joinings and nuclease attacks. ...
Extensions and Exceptions to Mendel`s Laws Sponge
Extensions and Exceptions to Mendel`s Laws Sponge

... expression of many symptoms in a disorder. These symptoms may be variably expressed • Occurs when a single protein affects different parts of the body or participates in the different biochemical processes ...
Tool 1
Tool 1

... similar, the typists may talk of one or two “band-differences” and sometimes not be sure if the isolates are in fact very similar after all). To be sure that identical band patterns represent identical isolates, it’s best to perform the analysis using different restriction enzymes (two, more rarely ...
Non-narrated powerpoint - Southern Adventist University
Non-narrated powerpoint - Southern Adventist University

... To help you understand the information in this presentation, it would be helpful for you to watch these first: • Origins 101 (1-3) • DNA, Chromosomes, and Genes • Mutations 1 • Mutations 2 ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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