Chapt 20 DNA Replication I: Basic Mechanism and Enyzmology
... • Describe general features of semi-conservative DNA replication: leading, lagging, strands; requirement for primers; bidirectional, rolling circle • Describe DNA polymerases: general enzymology and comparison of prokaryotes, eukaryotes • Describe major types of DNA damage and repair: ...
... • Describe general features of semi-conservative DNA replication: leading, lagging, strands; requirement for primers; bidirectional, rolling circle • Describe DNA polymerases: general enzymology and comparison of prokaryotes, eukaryotes • Describe major types of DNA damage and repair: ...
DNA Sequencing and Gene Analysis
... When looking across different species, most DNA sequences are not conserved. However, the exons of genes are often highly conserved, because their function is necessary for life. Zoo blot: a Southern blot containing genomic DNA from many species. Probe it with the sequence in question: exons will hy ...
... When looking across different species, most DNA sequences are not conserved. However, the exons of genes are often highly conserved, because their function is necessary for life. Zoo blot: a Southern blot containing genomic DNA from many species. Probe it with the sequence in question: exons will hy ...
Biotechnology Vocabulary
... 1) Selective breeding = The process by which desired traits of certain plants and animals are selected and passed on to their future generations. 2) Human Genome Project = (1988-2003) the mapping and sequencing of all the genes in the human genome 3) Genome is the total DNA in the nucleus of each ce ...
... 1) Selective breeding = The process by which desired traits of certain plants and animals are selected and passed on to their future generations. 2) Human Genome Project = (1988-2003) the mapping and sequencing of all the genes in the human genome 3) Genome is the total DNA in the nucleus of each ce ...
Biotechnology student NOTES
... 1) Selective breeding = The process by which desired traits of certain plants and animals are selected and passed on to their future generations. 2) Human Genome Project = (1988-2003) the mapping and sequencing of all the genes in the human genome 3) Genome is the total DNA in the nucleus of each ce ...
... 1) Selective breeding = The process by which desired traits of certain plants and animals are selected and passed on to their future generations. 2) Human Genome Project = (1988-2003) the mapping and sequencing of all the genes in the human genome 3) Genome is the total DNA in the nucleus of each ce ...
Indezine Template
... • During transcription, one of the two DNA strands called the template strand provides a template for ordering the sequence of nucleotides in an RNA transcript • During translation, the mRNA base triplets, called codons, are read in the 5 to 3 direction • Each codon specifies the order of amino ac ...
... • During transcription, one of the two DNA strands called the template strand provides a template for ordering the sequence of nucleotides in an RNA transcript • During translation, the mRNA base triplets, called codons, are read in the 5 to 3 direction • Each codon specifies the order of amino ac ...
DNA: The molecular basis of mutations
... Little mutations with big effects: Mutations to control genes Mutations are often the victims of bad press — unfairly stereotyped as unimportant or as a cause of genetic disease. While many mutations do indeed have small or negative effects, another sort of mutation gets less airtime. Mutations to c ...
... Little mutations with big effects: Mutations to control genes Mutations are often the victims of bad press — unfairly stereotyped as unimportant or as a cause of genetic disease. While many mutations do indeed have small or negative effects, another sort of mutation gets less airtime. Mutations to c ...
Chapter 13 The Genetics of Viruses and Prokaryotes
... is encoded by a gene designated (in humans) PRNP located on our chromosome 20 ...
... is encoded by a gene designated (in humans) PRNP located on our chromosome 20 ...
Educational Items Section Nomenclature for the description of mutations and
... on cDNA, A in 123 is replaced by G ...
... on cDNA, A in 123 is replaced by G ...
11. Origin and evolution of life (part I)
... ② Formation of complex organic molecules capable of self-replication (RNA, DNA) The next step toward the emergence of life is the formation of complex organic molecules (polymers like RNA consisting of a chain of nucleotides). RNA is similar to DNA but instead of being double-stranded and helical, i ...
... ② Formation of complex organic molecules capable of self-replication (RNA, DNA) The next step toward the emergence of life is the formation of complex organic molecules (polymers like RNA consisting of a chain of nucleotides). RNA is similar to DNA but instead of being double-stranded and helical, i ...
Human Identity Testing
... that carries a “lightbulb.” The lightbulb is an analogy for a radioactive label or fluorescent dye that allows it to be visible. The probe is allowed to bind (aka hybridize) with its complementary section in the medium. Then special procedures are used to wash away any remaining single stranded prob ...
... that carries a “lightbulb.” The lightbulb is an analogy for a radioactive label or fluorescent dye that allows it to be visible. The probe is allowed to bind (aka hybridize) with its complementary section in the medium. Then special procedures are used to wash away any remaining single stranded prob ...
Lindsay Kinyon
... the octahedrally coordinated platinum family analogs, [Pt(N-N)3]4+ & [Pd(N-N)3]4+, most likely due to the difficulty of solvating the high charge density of the platinum (4+) & palladium (4+) ions. Early in the summer of 1996, Dr. Robert Granger developed a new, versatile scheme for the synthesis of ...
... the octahedrally coordinated platinum family analogs, [Pt(N-N)3]4+ & [Pd(N-N)3]4+, most likely due to the difficulty of solvating the high charge density of the platinum (4+) & palladium (4+) ions. Early in the summer of 1996, Dr. Robert Granger developed a new, versatile scheme for the synthesis of ...
Meiosis and Mitosis
... 1. Mitosis occurs during cell division of somatic cells. A diploid parent cell generates two identical diploid daughter cells. Normally, recombination (discussed later) does not take place. 2. Meiosis is the process by which sex cells (gametes) are formed. A diploid progenitor cell generates fo ...
... 1. Mitosis occurs during cell division of somatic cells. A diploid parent cell generates two identical diploid daughter cells. Normally, recombination (discussed later) does not take place. 2. Meiosis is the process by which sex cells (gametes) are formed. A diploid progenitor cell generates fo ...
Genetic Engineering - Valhalla High School
... identify the sequence of bases in a DNA molecule make unlimited copies of DNA ...
... identify the sequence of bases in a DNA molecule make unlimited copies of DNA ...
Chapter 11
... By altering the sequence, a ribozyme can be engineered to cleave any mRNA sequence. ...
... By altering the sequence, a ribozyme can be engineered to cleave any mRNA sequence. ...
Chapter 1
... especially if internal organs such as the liver, lungs, or kidneys have been damaged. One method of treatment involves injecting a blood-clotting factor that has been purified from blood donations. This factor is a protein encoded by a human gene. Suggest a way in which modern genetic technology cou ...
... especially if internal organs such as the liver, lungs, or kidneys have been damaged. One method of treatment involves injecting a blood-clotting factor that has been purified from blood donations. This factor is a protein encoded by a human gene. Suggest a way in which modern genetic technology cou ...
Molecular Biology-restrection enzyme
... • Each species of bacteria has its own set of restriction enzymes. Each enzyme cuts DNA at a specific short base sequence. For instance, EcoR1 cuts the DNA at the sequence GAATTC, and BamH1 cuts at GGATCC. There are hundreds of restriction enzymes known. • Using properly chosen enzymes, the gene you ...
... • Each species of bacteria has its own set of restriction enzymes. Each enzyme cuts DNA at a specific short base sequence. For instance, EcoR1 cuts the DNA at the sequence GAATTC, and BamH1 cuts at GGATCC. There are hundreds of restriction enzymes known. • Using properly chosen enzymes, the gene you ...
Chapter 14 Biotechnology and Genomics
... •The restriction enzyme is used to cut DNA at specific points during production of rDNA. •It is called a restriction enzyme because it restricts growth of viruses but it acts as a molecular scissors to cleave any piece of DNA at a specific site. •Restriction enzymes cleave vector (plasmid) and forei ...
... •The restriction enzyme is used to cut DNA at specific points during production of rDNA. •It is called a restriction enzyme because it restricts growth of viruses but it acts as a molecular scissors to cleave any piece of DNA at a specific site. •Restriction enzymes cleave vector (plasmid) and forei ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.