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Medical Applications of Bioinformatics
Medical Applications of Bioinformatics

... Tools for Finding Motifs • Define a motif from a set of known proteins that share a similar sequence and function. • A pattern is a list of amino acids that can occur at each position in the motif. • A profile is a matrix that assigns a value to every amino acid at every position in the ...
Purification/UV-Vis Analysis Polymerase Chain Reaction (PCR
Purification/UV-Vis Analysis Polymerase Chain Reaction (PCR

... virginianus) as a means to track maternal and paternal breeding history within various populations located in Ohio and Pennsylvania. Thirty-three samples, representing a large variety in terms of age and sex, were procured via parks. DNA was originally obtained from liver tissue, but the experimenta ...
Unit 1 content check list
Unit 1 content check list

... Give examples of each main form of protein shape (fibrous, globular, conjugated) Explain the need for cellular differentiation Describe how plants (meristems) and animals (stem cells) form specialised cells Describe the difference between; pleuripotent, totipotent and differentiated Give examples of ...
4.04 Workfile
4.04 Workfile

... But out of all the methods, the most reliable forensic technique police use is called DNA fingerprinting. As opposed to traditional fingerprinting in which the actual fingerprints are lifted from the crime scene, this type of fingerprinting looks inside the cell of a human being to determine who com ...
Regulation of Bovine Parathyroid Hormone (Pth) Gene Expression
Regulation of Bovine Parathyroid Hormone (Pth) Gene Expression

... hybridisation of latently infected cultures demonstrates the presence of at least 1 copy of HSV DNA per cell, present in a form which lacks genanic termini and characteristic of the latent genane in vivo. The creation of other mutants possessing the B-galactosidase gene has confirmed these findings. ...


... chromosome of >20 Mb interstitially or >10 Mb telomerically (15 and 8 Mb, respectively, for imprinted chromosomes). * Contiguous homozygosity of >8 Mb within multiple chromosomes suggests common descent. These regions of potential recessive allele risk are designated. * A high level of allele homozy ...
THE STUDY OF HERITABLE CHANGES IN GENE FUNCTION THAT
THE STUDY OF HERITABLE CHANGES IN GENE FUNCTION THAT

Activator Proteins
Activator Proteins

... • first level of DNA packing • histone proteins • 8 protein molecules • many positively charged amino acids • bind tightly to negatively charged DNA ...
File - Mr Andrews` Science Space!
File - Mr Andrews` Science Space!

... NCEA information 1.9 • 5 Biological ideas relating to genetic variation are limited to concepts and processes connected with: • • the continuity of life based on the inheritable nature of DNA • • links between DNA and variation in phenotypes • • variation in phenotypes as adaptive features. • 6 Bio ...
Genetic (molecular) Markers and their uses
Genetic (molecular) Markers and their uses

... Stands for random amplifica7on of polymorphic DNA. It is a type of PCR reac6on, but the segments of DNA that are amplified are random. The scien6st performing RAPD creates several arbitrary, short primers (8–12 nucleo6des), then proceeds with the PCR using a large ...
Tinkering with the Biochemistry of Life: Viruses, Prions, and Peptide
Tinkering with the Biochemistry of Life: Viruses, Prions, and Peptide

PATENT PROTECTION FOR GENE SEQUENCES WHAT IS
PATENT PROTECTION FOR GENE SEQUENCES WHAT IS

... • the next step scientists are taking is to study what function each part of a gene performs – if any, because it is recognised that some parts may not have any function. Particularly biotech companies are keen to figure out what effect each part has on the organism. Therefore, as today’s technology ...
File
File

... RNA. The RNA strand detaches from the DNA as it is transcribed, and the DNA zips back together. mRNA: intermediate message that is translated to form a protein; rRNA: forms part of ribosomes; tRNA: brings amino acids from the cytoplasm to a ribosome to help make the growing protein Both occur within ...
Bchem 4200 Part13 - U of L Class Index
Bchem 4200 Part13 - U of L Class Index

... → Leaving the target side might also involve sliding etc. Sliding accelerates target site location: → under optimum conditions it allows for scanning of ~106 bases per binding event. → but it’s a random walk →the effective sliding distance is much shorter ~ 1000 bp → ionic conditions, in particular ...
AMP v. Myriad – Brief History
AMP v. Myriad – Brief History

... WARF argues that claim recites “in vitro cell culture” – clearly not a “product of nature” Expert witness for WARF further testified that the morphology of the hESCs are different from prior art mouse ES cell colonies (hESC colonies are flatter and more compact) CW argues that the claims “merely ide ...
Document
Document

... Fig 12.15 ...
- Flat Rock Community Schools
- Flat Rock Community Schools

... allele (P) produces purple color, and the recessive allele (p) produces yellow color. The diagram below shows an ear of corn produced by crossing two corn plants. The shaded kernels are purple, and the unshaded ones are yellow. What can the yellow kernels best be described as? (Be sure to look at wh ...
Transcription
Transcription

... It is like DNA replication in that a DNA strand is used to synthesize a strand of mRNA. Only one strand of DNA is copied. A single gene may be transcribed thousands of times. After transcription, the DNA strands rejoin. Steps involved in transcription RNA polymerase recognizes a specific base sequen ...
The genetic code and the “central dogma` Genetic information and
The genetic code and the “central dogma` Genetic information and

... biomolecules such as proteins and nucleic acids - The membrane is a phospholip bilayer with embedded proteinic structures - Terrestrial life is characterized by two different types of cells: prokaryotic and eukaryotic ...
Bacterial Nucleic Acids
Bacterial Nucleic Acids

... • DNA---Chromosomes---Genes • Genes –small sequences of DNA • Carries all information for –development and function • Their information is used to make protein with the help of RNA through Transcription...Translation. • The DNA double helix is stabilized by hydrogen bonds between the bases attached ...
Gene Expression
Gene Expression

... Transcription is the process of creating RNA from DNA. Transcription occurs in the cell's nucleus. RNA polymerase is the protein molecule that reads the DNA and creates the RNA intermediary. Transcription requires: DNA, RNA polymerase, ribonucleotides, and some ATP for energy. Uracil (U) is substitu ...
Section 9 – Human therapeutics and forensic uses
Section 9 – Human therapeutics and forensic uses

... evidence proved anything. Samples could be contaminated easily. ...
o"', ,jl w - 'J'
o"', ,jl w - 'J'

... GAII / GAIII / SF/ FC ...
Weldon_McVean - Wellcome Trust Centre for Human Genetics
Weldon_McVean - Wellcome Trust Centre for Human Genetics

... Polymorphic in humans – leads to variation in hotspots and genome instability ...
Decode the following message.
Decode the following message.

... removed from a DNA sequence at single point. • An deletion of one base pair causes a shift in the reading frame = One or more amino acids changed Base Pair Removed ...
< 1 ... 406 407 408 409 410 411 412 413 414 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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