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Summary of sixth lesson
Summary of sixth lesson

... – Our sample is either 10 or 20 – Calculate whether each new sample is characterized by new alleles ...
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Genetics exam 4

... _____The genes coding for histones are repeated several times throughout the eukaryotic genome. These genes would be described as A. Highly repetitive DNA B. Unique sequences C. Heterochromatin D. Transposons E. Middle repetitive DNA _____ A chromosome with its centromere in the middle is a A. Subm ...
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No Slide Title

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... to produce offspring with similar traits • Possible problems: “bad” genes are also passed to the new generation ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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