• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Study Guide - Flagler Schools
Study Guide - Flagler Schools

... Understand  how  fossil  evidence  provides  support  for  the  theory  of  evolution.       Understand  the  process  of  natural  selection     Know  the  difference  between  the  lysogenic  and  lytic  cycle.       Understand  bacte ...
Exploring the role of TGF-β signaling in Mouse
Exploring the role of TGF-β signaling in Mouse

Designer Genes - Heredity
Designer Genes - Heredity

... chromosomal DNA within a cell as a bacterium When used in genetic engineering – called vectors Several methods to isolate plasmid DNA from bacteria ...
Biology  6 Test 2 Study Guide
Biology 6 Test 2 Study Guide

... a. Recombinant DNA technology – genes mixed from different organisms. i. Create new strains, or produce a product (Fig. 9.1) ii. Restriction enzyme cloning (Fig. 9.2) 1. Restriction enzymes cut DNA at specific sites. Can produce “sticky ends” that can base pair to other sticky ends. (Tab. 9.1) 2. DN ...
mutation and recombination as one nucleotide pair
mutation and recombination as one nucleotide pair

... pages, which opens with a discussion of the relationship between genotype and phenotype for quantitative characters. The author then disposes of the notoriously difficult problem of scales and scaling tests in one page. The partitioning of variation between additive and dominance components using a ...
Step 2
Step 2

... Example* - Pipeline for Analysis of Protein Variation Due to Alternative Splicing and SNPs The alternative splicing pipeline will provide a complete characterization of variations in proteins due to splice variation or SNPs evident in repositiories of contiguous genome sequence data and expressed s ...
Soybean (Glycine max L.) Nuclear DNA Contains
Soybean (Glycine max L.) Nuclear DNA Contains

Into the Flame: The Cremated Bones of Early Roman Period at
Into the Flame: The Cremated Bones of Early Roman Period at

... During the archaeological research in Lobor in 2011, three different time phases of the cemetery burials were defined at the east, outer part of the shrine of Our Lady of the Mountain. The third, youngest and most intriguing burial phase includes individual burials, the graves of five persons which ...
plasmid vector
plasmid vector

... 7. The relaxosome, consisting of Y, M, l and hostencoded lHF bound to the nicked DNA in oriT is shown interacting with the coupling protein, D, which in turn interacts with B. 8. The 5’ end of the nicked strand is shown bound to a tyrosine (Y) in l, and the 3’ end is shown being associated with l in ...
Sem 2 Bio Review Questions
Sem 2 Bio Review Questions

... spots (Y) is dominant over the gene for red spots and the gene for blue skin color (B) is dominant over the gene for green skin color. What is the probability that red spotted, green skinned offspring will be produced in a cross between a parent that has green skin and is heterozygous (hybrid) for y ...
power pack 5 dna replication
power pack 5 dna replication

... 10. In proof reading during DNA replication a. wrong nucleotides are inserted b. wrong nucleotides are taken out c. wrong nucleotides are removed and correct ones are inserted d. mutations are prevented 11. E.coli fully labeled with N15 is allowed to grow in N14 medium. The two strands of DNA molecu ...
Chapter 1 – The nature of science
Chapter 1 – The nature of science

... What is a physical property? Examples of physical properties. What are chemical properties? Examples of physical and chemical changes. The atom is the basic unit of matter The atom o Subatomic particles o The make up of the nucleus of the atom o What are Protons, neutrons and electrons? o Atomic num ...
Lecture 10
Lecture 10

... I hope I have convinced you in the last four lectures that once life arises, evolution will allow it to diversify and become better adapted to its environment. This doesn’t guarantee anything in particular about progress towards intelligence. Indeed, development of intelligence or even complex life ...
The Big Picture: an outline of the concepts covered to date
The Big Picture: an outline of the concepts covered to date

... A. Each diploid individual contains two copies of a given gene B. Each Gene can have different forms called alleles. There are two alleles in a diploid individual The form that is expressed phenotypically in the heterozygote is known as the dominant allele. It is an operational definition C. These c ...
CHAPTER 14
CHAPTER 14

... TFIID and TFIIB would be slightly greater than this length. Therefore, if the DNA was in a nucleosome structure, these proteins would have to be surrounding a nucleosome. It is a little hard to imagine how large proteins such as TFIID, TFIIB, and RNA polymerase II could all be wrapped around a singl ...
Unit 08 Notes - Pierce College
Unit 08 Notes - Pierce College

... except smaller. In humans, each somatic (body) cell has 46 chromosomes, 23 pairs. At the beginning of mitosis, these 46 chromosomes consist of doubled (replicated) DNA molecules, so there are 92 DNA molecules total. Each DNA molecule has supercoiled into chromatids, so there are 92 chromatids, held ...
Genetics - Tour of the Basics
Genetics - Tour of the Basics

... Look at this set of chromosomes. You can see that matching chromosomes have been lined up in pairs – one each from mom and dad. Although the DNA double helix is too small to see, chromosomes can be viewed with a _______________, as in this picture. There are __________ sex chromosomes that determine ...
DNA: The Molecule of Heredity How did scientists discover that
DNA: The Molecule of Heredity How did scientists discover that

... • When cells divide, the DNA must be copied so each daughter cell receives an exact copy. • A cell must: – Replicate its DNA exactly one time before division – Divide after DNA replication – Have energy to do both ...
Chapter 9
Chapter 9

... paternally derived genes are absent or disrupted, the PWS phenotype results. When this same segment is missing from the maternally derived chromosome 15, a completely different disease, Angelman syndrome, arises. This pattern of inheritance when expression of a gene depends on whether it is inherite ...
RNA
RNA

...  rRNA- is a single strand in globular form, rRNA binds with proteins to make up ribosomes which are then used to make the proteins ...
14–3 Human Molecular Genetics
14–3 Human Molecular Genetics

... The results of the Human Genome Project have led to the possibility of using gene therapy to treat genetic disorders. Gene therapy involves replacing a defective or missing gene in a person’s genome. ...
DNA, RNA, and Proteins
DNA, RNA, and Proteins

... b.  Ribosome is free to begin translation again with another mRNA ...
Chapter 3 – Cell Structure and Function
Chapter 3 – Cell Structure and Function

... Transcription: process of making mRNA. RNA is basically a copy of DNA, and this copy is used to make proteins that DNA codes for. RNA Polymerase: enzyme that binds to DNA and makes mRNA. mRNA (messenger RNA) carries code from nucleus down to ribosomes on the Rough ER. rRNA (ribosomal RNA) is the sit ...
Abstract Microbial source tracking (MST) is a powerful emerging
Abstract Microbial source tracking (MST) is a powerful emerging

... Four different approaches to MST methods are currently being developed. The first uses a database or “library” of known sources of fecal bacteria (i.e., humans, livestock, companion animals, wildlife, etc.) that are “fingerprinted” by a variety of biochemical or molecular protocols. Fecal bacteria o ...
Chapters 18, 19, 20, 27) Virus, bacteria, gene expression
Chapters 18, 19, 20, 27) Virus, bacteria, gene expression

... In a process called DNA methylation, methyl groups are added to DNA bases (usually cytosine) Genes are more heavily methylated in cells in which they are not expressed. Some proteins that bind to methylated DNA also interact with histone deacetylation enzymes, reinforcing the transcription repressio ...
< 1 ... 346 347 348 349 350 351 352 353 354 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report