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Biological Basis for Gene Hunting
Biological Basis for Gene Hunting

... human genome, but they were not easy to detect. Lab techniques such as flourescent in-situ hybridization or FISH (see Section X.X) had been used for some time to detect very specific chromosomal microdeletions. (A microdeletion is a deletion of a large section of DNA, but one too small to see throug ...
pARA and pKAN-R
pARA and pKAN-R

... The plasmid pARA is 4058 base pairs (bp) in size. A “base pair” would be adenine:thymine or guanine:cytosine and is the common method used to express the size of DNA molecules. The plasmid carries the ampr gene, which encodes the protein beta lactamase, an enzyme that destroys the antibiotic ampicil ...
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Exam 2a - web.biosci.utexas.edu
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Computer programs for the analysis and the management of DNA
Computer programs for the analysis and the management of DNA

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of the protein - Lighthouse Christian Academy

... keratinization. As this occurs, the hair cells die. The dead cells and keratin form the shaft of the hair. 3. Fingernails grow about three or four times as quickly as toenails 4. Each hair grows about 1/4 inch/month and grows for up to 6 years. 5. The most expressive muscles are the facial muscles. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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